• Environmental Analysis Health and Toxicology
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• v.24 no.2
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• pp.107-117
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• 2009

Metal pollution of aquatic ecosystems is a problem of economic and health importance. Information regarding molecular responses to metal exposure is sorely needed in order to identify potential biomarkers. To determine the effects of heavy metals on chironomids, the full-length cDNA of alcohol dehydrogenase (ADH3) from Chironomus riparius was determined through molecular cloning and rapid amplification of cDNA ends (RACE). The expression of ADH3 was analyzed under various cadmium and copper concentrations. A comparative and phylogenetic study among different orders of insects and vertebrates was carried out through analysis of sequence databases. The complete cDNA sequence of the ADH3 gene was 1134 bp in length. The sequence of C. riparius ADH3 shows a low degree of amino acid identity (around 70%) with homologous sequences in other insects. After exposure of C. riparius to various concentrations of copper, ADH3 gene expression significantly decreased within 1 hour. The ADH3 gene expression was also suppressed in C. riparius after cadmium exposure for 24 hour. However, the effect of cadmium on ADH3 gene expression was transient in C. riparius. The results show that the suppression of ADH3 gene by copper exposure could be used as a possible biomarker in aquatic environmental monitoring and imply differential toxicity to copper and cadmium in C. riparius larvae.

• Archives of Pharmacal Research
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• v.27 no.4
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• pp.422-428
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• 2004

Expressed sequence tags (ESTs) were generated from two 3'-directed CDNA libraries constructed from quiescent and activated rat hepatic stellate cell (HSC) to analyze the expression profiles of active genes in both cells. From quiescent and activated HSC, 694 ESTs and 779 ESTs, respectively, were obtained after excluding those having shorter than 30 bp. Amonq ESTs obtained from quiescent and activated HSC, 68 and 73 kinds of ESTs (186 clones and 236 clones), respectively, appeared more than once, implying that their genes are expressed highly in each cell type. 52 among 73 ESTs appeared only in the activated HSC 47 amonq 68 ESTs only in the normal HSC, and 21 in both cells. The genes of these 52 ESTs were assumed to be expressed more highly in the activated HSC. To confirm the high expression of genes of which the ESTs appeared more than twice in the activated HSC, northern hybridization was carried out with RNAs derived from rat normal and fibrotic liver using each of 18 EST DNAs as probe. 13 ESTs showed more intense bands with RNA isolated from the fibrotic liver than normal liver. From these results, we confirm the positive correlation between abundance of transcript in activated HSCs and the expression level in fibrotic liver, The expression profile of the transcripts serves as an important tool in understanding the biological properties of HSC.

• 제어로봇시스템학회:학술대회논문집
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• 2000.10a
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• pp.470-470
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• 2000

This paper describes a new method of identifcation of backlash nonlinear systems by use of M-sequence correlation method. In this method, we can obtain not only Volterra kernels of up to 3rd order of the nonlinear system, but also the width of the backlash element from observing the crosscorrelation between the input and the output. Here strictly speaking, a multi-valued nonlinear system such as backlash element can not be expressed by Volterra kernel representation mathematically. But in practice, we encounter many cases where it is difficult to treat them mathematically but they can be controlled from experience. So we here dare to suppose that backlash nonlinear system can be approximated by Volterra kernel representation. Simulations are carried out on a nonlinear system consisting of linear part plus backlash element. And Volterra kernels are measured. The output calculated from the observed Volterra kernels is in good agreement wi th the actual output. And we show that we can obtain the width of backlash element, which is one of the most important parameters, by observing the maximum value of crosscorrelation function between the input M-sequence and the output.

• Korean Journal of Veterinary Research
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• v.60 no.3
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• pp.109-116
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• 2020

This study aimed to develop a semi-nested polymerase chain reaction assay for the direct detection of Mycoplasma synoviae (M. synoviae) from clinical samples using three newly designed oligonucleotide primers specific to the variable lipoprotein haemagglutinin (vlhA) gene and differentiate M. synoviae field strains based on a nucleotide deletion or the insertion of the proline-rich repeat (PRR) region of the vlhA gene. The developed semi-nested polymerase chain reaction (PCR) assay revealed positive results in 12 out of 100 clinical samples collected from chickens showing lameness and joint swelling. Six positive samples were selected randomly for sequencing, and sequence analysis revealed 96.3-100% nucleotide identities compared to the reference sequences. Phylogenetic analysis showed that sequences of the strains in this study were closely related to WVU1853 (Spain), CK.MS.UDL.PK.2014.2 (Pakistan), and F10-2AS (USA) strains, but they were distinct from the M. synoviae-H vaccine strain sequence. M. synoviae obtained from these samples were identified as types A and C with a length of 38 and 32 amino acids, respectively. These results indicated that the specific and sensitive semi-nested PCR could be a useful diagnostic tool for the direct identification of clinical samples, and the sequence analysis of the partial vlhA gene can be useful for typing M. Synoviae.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2011.11a
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• pp.16-18
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• 2011

This study aims to identify the degree of safety when mariners take their actions in several different situations. We have carried out many experiments in order to observe mariners' behavior, and then measured the safety level that is based on their actions to avoid dangerous collision situations. One of the most important actions that mariners have to take, either as their daily routine or when they are in a collision situation and then want to avoid that situation is the lookout. In this paper, behaviors on the lookout have been defined as a standard sequence of three steps that are "time of first detection", "time of recognition as risky vessel" and "time of starting avoiding action", and the suitability and applicability of the definition have been shown. And also we propose the risk assessment on the collision and the recommendation for reducing the collision at sea. Some analyzing results and the application of the results are reported. The sequence of lookout is also understood. By combining these knowledge and some systematic studies, we propose the risk assessment on the collision and the recommendation for reducing the collision at sea.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2005.09a
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• pp.388-391
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• 2005

The Protein Structural and Functional Conservation need a common language for data definition. With the help of common language provided by Protein Ontology the high level of sequence and functional conservation can be extended to all organisms with the likelihood that proteins that carry out core biological processes will again be probable orthologues. The structural and functional conservation in these proteins presents both opportunities and challenges. The main opportunity lies in the possibility of automated transfer of protein data annotations from experimentally traceable model organisms to a less traceable organism based on protein sequence similarity. Such information can be used to improve human health or agriculture. The challenge lies in using a common language to transfer protein data annotations among different species of organisms. First step in achieving this huge challenge is producing a structured, precisely defined common vocabulary using Protein Ontology. The Protein Ontology described in this paper covers the sequence, structure and biological roles of Protein Complexes in any organism.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.9 no.1
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• pp.242-259
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• 2015

Three-dimensional video coding is one of the main challenges restricting the widespread applications of 3D video and free viewpoint video. In this paper, a novel fractal coding algorithm with motion-vector-field-based motion estimation for depth map sequence is proposed. We firstly add pre-search restriction to rule the improper domain blocks out of the matching search process so that the number of blocks involved in the search process can be restricted to a smaller size. Some improvements for motion estimation including initial search point prediction, threshold transition condition and early termination condition are made based on the feature of fractal coding. The motion-vector-field-based adaptive hexagon search algorithm on the basis of center-biased distribution characteristics of depth motion vector is proposed to accelerate the search. Experimental results show that the proposed algorithm can reach optimum levels of quality and save the coding time. The PSNR of synthesized view is increased by 0.56 dB with 36.97% bit rate decrease on average compared with H.264 Full Search. And the depth encoding time is saved by up to 66.47%. Moreover, the proposed fractal depth map sequence codec outperforms the recent alternative codecs by improving the H.264/AVC, especially in much bitrate saving and encoding time reduction.

• Animal cells and systems
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• v.13 no.4
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• pp.381-389
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• 2009

Using the DDRT-PCR, a series of differentially expressed genes in human primary cervical cancer was isolated. Among the 250 PCR amplimers, 88 gene fragments were confirmed by reverse Northern hybridization. Homology searches indicated that 26 out of 88 were previously known genes including calmodulin, human BBC1, histone H3.3, a series of ribosomal proteins (RPL19, RPS19, and RPS12), translation initiation factor (eIF-4AI), lactoferrin, integrin ${\alpha}6$, cell-surface antigens (CD9 and CD59), transcription factor (mbp-1), and mitochondrial proteins. Several unknown clones showed sequence homology with known genes. Furthermore, six of the unknown genes showed identical sequence with expressed sequence tags (EST) of unknown function. Differential expression patterns of identified genes were further examined and confirmed with multiple pairs of cervical cancer samples using Northern hybridization. Our profiling of differentially expressed genes may provide useful information about the underlying genetic alterations in human cervical carcinoma and diagnostic markers for this disease. The precise roles of these genes in cancer development remain to be elucidated.

• The Journal of the Korea Contents Association
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• v.11 no.3
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• pp.65-72
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• 2011

We stand at real world that some practical use method of gene information appears in succession by entrance on the stage of advanced techonlogy. As a lot of studies and development are achieved based on analysis of bio data, necessity of a tool that can help correct interpretation of data is required more and more in a lot of targets of bioinformatics to search new relation and information are established. In this paper, we are offered in existing I wish to offer user a more convenient study tool developing system that can supplement shortcomings of various tools for data analysis. So we've designed to offer in united environment that is not environment that is parted ORF driving out, bio information retrieval and work of similarity comparison lamp to work for bio data analysis and offers lacking consecutiveness in existing analysis system.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.23 no.6
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• pp.546-552
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• 2013

A cantilevered piezoelectric energy harvester(PEH) and an auxiliary mass-spring unit can be integrated into a novel two-degrees-of-freedom PEH where its lowest eigenmode is not an in-phase modes but an out-of-phase mode. This typical behavior was shown to enhance output power considerably compared with its stand-alone counterpart. The objective of this study is to newly develop a continuum-based mathematical model suitable for efficient analysis of the mode-sequence reversed PEH. Once such a mathematical model is available, various physical behaviors can be analytically investigated for better designs. After a new mathematical model is developed, its validity is checked by using ANSYS results, in terms of resonant frequency, open-circuit voltage, and output power with a specified external resistance.