• Journal of Forest and Environmental Science
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• v.34 no.3
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• pp.224-234
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• 2018

An efficient method to select drought tolerant Korean native plants using in vitro culture system was established in this study. While the plant growths and root inductions of each plant were proportionately affected by concentrations of mannitol on in vitro culturing seven plant species to test tolerance to osmotic stress, growth index (GI) and number of root induction of Chrysanthemi zawadskii var. latilobum and Dianthus chinensis var. semperflorens plantlets were higher than the others in 125mM mannitol. In test with polyethylene glycol (PEG), plantlets of C. zawadskii var. latilobum and D. chinensis var. semperflorens showed higher GI and number of root induction than the others in 33.3mM. On testing whether the well grown plants under osmotic stress are tolerant to virtual drought stress, there were significant differences in the withering rates of C. zawadskii var. latilobum and D. chinensis and those of were Aster yomena and Centaurea cyanus after 12 days without watering. It was found that significantly lower stomata numbers were shown in both drought tolerant plants than the sensitive plants. Averages of the stomata circumferences and the stomata area in the plantlets of the tolerant species were larger than those of the sensitive plants D. chinensis var. semperflorens showed the lowest transpiration level per unit area. The highest stomatal area per unit area was found in C. zawadskii, followed by D. chinensis var. semperflorens, Aster yomena and C. cyanus. In conclusion, C. zawadskii var. latilobum and D. chinensis var. semperflorens were more tolerant to drought than other two species. Furthermore in vitro selection was successfully used to screen drought tolerance species of native plant species.

• Plant Resources
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• v.8 no.3
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• pp.202-208
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• 2005

Osmotic stress is one of major limiting factors in crop production. In particular, seasonal drought often causes the secondary disease in the field, resulting in severe reduction in both quality and productivity. Recent efforts have revealed that many genes encoding protein kinases play important roles in osmotic stress signal transduction pathways. Previously, the AtSIK (Arabidopsis thaliana Stress Inducible Kinase) mutants have shown to enhance tolerance to abiotic stresses, accompanying with higher expression of abiotic stress-related genes than did the wild-type plants. In this study, we have transformed potato (cv. Taedong Valley) with the AtSIK expression cassette. Both PCR and RT-PCR using AtSIK-specific primers showed stable integration and expression of the AtSIK gene in individual transgenic lines, respectively. Foliar application of herbicide ($Basta^{(R)}$) at commercial application rate (0.3% (v/v)) revealed another evidence of stable gene introduction of T-DNA which includes the bar gene for herbicide resistance. Overexpression of the AtSIK gene under dual CaMV35S promoter increased sensitivity to salt stress (300 mM NaCl), which was demonstrated by the reduction rate of chlorophyll contents in leaves of transgenic potato lines. These results suggest that possible increase of osmotic tolerance in potato plants may be achieved by antisense expression of AtSIK gene.

• Korean Journal of Organic Agriculture
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• v.24 no.2
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• pp.273-287
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• 2016

Accumulation of excessive salt in Reclaimed coastal tidelands can reduce crop yields, reduce the effectiveness of irrigation, degradation of soil structure, and affect other soil properties. These salts has shown to cause specific ions in the plant over a period of time leads to ion toxicity or ion imbalance and a continuous osmotic phase that prevents water uptake by plants due to osmotic pressure of saline soil solution. This review focuses on the characteristics of salt-affected soils, mechanisms of salt-tolerance plants, desalinization technology, and soil management to maintain sustainable agro-ecosystem in salt-affected soils.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.3
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• pp.390-399
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• 2010

Non-photochemical quenching (NPQ) values for utilizing them to detect osmotic tolerance in plants were examined with two different soybean cultivars, an osmotic tolerant soybean (Shinpaldalkong 2) and a control soybean (Taekwangkong). Two different stresses were applied to the cultivars as the restricted irrigations of 200 and 50 ml water $pot^{-1}\;d^{-1}$ for 5 days for a control and a drought stress, respectively, and a sodium chloride solution of 200 mmol for 6 days for a salt stress. The intact leaves of the two cultivars after treatment were used to measure chlorophyll fluorescence parameters, maximum efficiencies of photosystem II photochemistry (Fv/Fm), efficiencies of photosystem II photochemistry (${\Phi}_{PSII}$), $CO_2$ assimilation rate ($P_N$), and NPQ. Leaf water potentials of the two cultivars decreased from - 0.2 to - 0.8MPa by a drought treatment and from - 0.7 to - 1.7MPa by a salt treatment. Leaf water content of Shinpaldalkong 2 after a salt treatment was less decreased than that of Taekwangkong. $F_v/F_m$ values of both cultivars were not changed, while ${\Phi}_{PSII}$ and $P_N$ were decreased proportionally to leaf water potential decrease. The response of NPQ was occurred in Shinpaldalkong 2 under the drought and salt stresses. With Taekwangkong cultivar, only drought stress referred NPQ response. The cultivar differences on chlorophyll fluorescence parameters were found in the relationships between ${\Phi}_{PSII}$ and $P_N$, and between NPQ and ${\Phi}_{PSII}$. Although the positive relationships between ${\Phi}_{PSII}$ and $P_N$ were established on all treatments of both cultivars, the decreasing rate of ${\Phi}_{PSII}$ to $P_N$ was smaller in Shinpaldalkong 2 than Taekwangkong. The NPQ was increased according to the decrease of ${\Phi}_{PSII}$ by osmotic treatments in Shinpaldalkong 2. The complementary relationships between NPQ and ${\Phi}_{PSII}$ were well maintained at all treatments in Shinpaldalkong 2, while these relationships were lost at a salt treatment in Taekwangkong. Taken together, the results suggest that analysis of complementary relationships between ${\Phi}_{PSII}$ and NPQ could be more valuable and applicable for determining osmotic tolerance than single analysis of each parameter such as $F_v/F_m$, ${\Phi}_{PSII}$ and NPQ.

• Membrane Journal
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• v.2 no.1
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• pp.33-48
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• 1992

OSRO process was developed and it was confirmed more effective in ethanol concentrating process comparing to reverse osmosis process. It may be industrialized if more effective membrane for OSRO and reverse osmosis, which indicate the value greater than zero, it was shown that OSRO process was more effective than reverse osmosis for the ethanol concentration process. The decrease of feed concentration and flow rate and the increase of applied pressure made more effective operating conditions in OSRO process to concentrate ethanol. From the numerical esults for the multi-plates, theoretical DC values of reverse osmosis and OSRO process was increased as the umber of stages increased. DC values were increased with the increase of applied pressure in same number of stages. The theoretical values of DC by numerical calculation were corresponded to the experimental values within 15% tolerance. DC value was increased proportional to applied pressure and osmotic sink solution flow rate but it was decreased proportional to feed concentration and flow rate. The numerical calculation over the wide ranges inclading experimental condition was proposed in this study.

• Journal of Korean Society of Forest Science
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• v.80 no.2
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• pp.210-219
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• 1991

This study is to diagnose the drought tolerance of twenty broad-leaved tree species by the pressure-volume(P-V) curves. As for the diagnosis of drought tolerance, the valuable water relations parameters obtained from P-V curves are the osmotic potential at full turgor, ${\Psi}_0{^{sat}}$, osmotic potential at incipient plasmolysis, ${\Psi}_0{^{tlp}}$, maximum bulk modulus of elasticity, $E_{max}$, and relative water content at incipient plasmolysis, $RWC^{tlp}$. Also, the figures related to the diagnosis of drought tolerance are the free water content (FWC) versus leaf water potential(${\Psi}_L$), volume-averaged turgor pressure ($P_{vat}$) versus leaf water potential (${\Psi}_L$), and H$\ddot{o}$fler diagram. In this study, the relatively high drought tolerant species are Fraxinus rhynchophylla, Quercus acutissima, Quercus serrata, Quercus aliena, and Populus alba${\times}$glandulosa ; the relatively low drought tolerant species are Fraxinus mandshurica, Betula platyphylla var. japonica, Populus euramericana, Kalopanax pictum, Carpinus loxiflora, Carpinus cordata, Prunus sargentii, Prunus leveilleana, and Cornus controversa ; medium species are Quercus mongolica, Acer mono, Acer triflorum, Acer pseudo-sieboldianum, Ulmus davidiana, and Zelkova serrata.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.81-84
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• 2000

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.127-127
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• 2005

• Journal of Life Science
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• v.9 no.1
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• pp.22-25
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• 1999

In order to study the mechanism for the adaptation to salt stress, we mutagenized budding yeast Saccharomyces cerevisiae with Ethylmethane sulfonate, and isolated salt-tolerant mutants. Among the salt-tolerant mutants, two strains exhibit additional temperature sensitive phenotype. Here, we report that these two salt-tolerant mutants are specific to {TEX}$Na^{+}${/TEX} rather than general osmotic stress. These mutant strains may contain mutations in the genes involved in {TEX}$Na^{+}${/TEX} home-ostasis.

• Mycobiology
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• v.39 no.1
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• pp.33-39
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• 2011

Wild yeasts on the surface of various fruits including grapes were surveyed to obtain yeast strains suitable for fermenting a novel wine with higher alcohol content and supplemented with rice starch. We considered selected characteristics, such as tolerance to alcohol and osmotic pressure, capability of utilizing maltose, and starch hydrolysis. Among 637 putative yeast isolates, 115 strains exhibiting better growth in yeast-peptone-dextrose broth containing 30% dextrose, 7% alcohol, or 2% maltose were selected, as well as five ${\alpha}$-amylase producers. Nucleotide sequence analysis of the 26S rDNA gene classified the strains into 13 species belonging to five genera; Pichia anomala was the most prevalent (41.7%), followed by Wickerhamomyces anomalus (19.2%), P. guilliermondii (15%), Candida spp. (5.8%), Kodamaea ohmeri (2.5%), and Metschnikowia spp. (2.5%). All of the ${\alpha}$-amylase producers were Aureobasidium pullulans. Only one isolate (NK28) was identified as Saccharomyces cerevisiae. NK28 had all of the desired properties for the purpose of this study, except ${\alpha}$-amylase production, and fermented alcohol better than commercial wine yeasts.