• BMB Reports
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• v.57 no.10
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• pp.441-446
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• 2024

The vascular system in plants facilitates long-distance transportation of water and nutrients through the xylem and phloem, while also providing mechanical support for vertical growth. Although many genes that regulate vascular development in rice have been identified, the mechanism by which epigenetic regulators control vascular development remains unclear. This study found that Rolled Fine Striped (RFS), a Chromodomain Helicase DNA-binding 3 (CHD3)/Mi-2 subfamily protein, regulates vascular development in rice by affecting the initiation and development of primordia. The rfs mutant was found to affect auxin-related genes, as revealed by RNA sequencing and reverse transcription-quantitative PCR analysis. The transcript levels of OsPIN1 and NAL1 genes were downregulated in rfs mutant, compared to the wild-type plant. The chromatin immunoprecipitation assays showed lower levels of H3K4me3 in the OsPIN1a and NAL1 genes in rfs mutant. Furthermore, exogenous auxin treatment partially rescued the reduced adventitious root vascular development in rfs mutant. Subsequently, exogenous treatments with auxin or an auxin-transport inhibitor revealed that the expression of OsPIN1a and NAL1 is mainly affected by auxin. These results provide strong evidence that RFS plays an important role in vascular development and root formation through the auxin signaling pathway in rice.

• 전자공학회논문지 IE
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• v.43 no.1
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• pp.46-51
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• 2006

Recently, smart card system which is known as easy to portable and also safe from physical, electrical, and software attack is observed to manage information that becomes the target of security in safety. And java card graft upon java technology to smart card platform is having very good advantage with object-oriented techniques and also, java card have the open type OS that can show the same action in different hardware characteristic which allows various application programs. In this paper, we introduced independent execution characteristic of java platform because being set to each smart card was uncomfortable till now and we designed access control member card that allows several administrators in different access privilege by single card using java card. Several administrators can approach to various information of file type that is included on issued card to user by using different PIN. In the proposed method, confirmation of personal information, administration contents update, demand by contents, is possible by single card. At this moment, wish to do safer user certification that improve security limitation which is from PIN, used for user certification, and signature data. In the proposed method, as design and implementation of utilization technology of java card, biometrics, user certification which uses multi PIN, provide that more safety and conveniently.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.89-96
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• 2017

The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgRNA design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritable-targeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgRNA, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species.