• Title/Summary/Keyword: OsCam1-1

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Calcium Signaling-mediated and Differential Induction of Calmodulin Gene Expression by Stress in Oryza sativa L.

  • Phean-o-pas, Srivilai;Punteeranurak, Pornpimon;Buaboocha, Teerapong
    • BMB Reports
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    • v.38 no.4
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    • pp.432-439
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    • 2005
  • $Ca^{2+}$/calmodulin transduction pathways have been implicated in mediating stress response and tolerance in plants. Here, three genes encoding calmodulin (Cam) members of the EF-hand family of $Ca^{2+}$-binding proteins were identified from Oryza sativa L. databases. Complementary DNA for each of the calmodulin genes, OsCam1, OsCam2, and OsCam3 were sequenced. OsCam1 and OsCam2 encode a conventional 148-amino acid calmodulin protein that contains four characteristic $Ca^{2+}$-binding motifs. OsCam3 encode a similar protein with a 38-amino-acid extension containing a putative prenylation site (CVIL) at the carboxyl terminus. RT-PCR showed that each of the genes is expressed in leaves and roots of 2-week old rice seedlings. By RNA gel blot analysis, OsCam1 mRNA levels strongly increased in response to NaCl, mannitol and wounding treatments. In contrast, OsCam2 mRNA levels were relatively unchanged under all conditions investigated. NaCl treatment and wounding also increased the OsCam3 mRNA level, but in a more transient manner. Our results indicate that although the expression of genes encoding different calmodulin isoforms is ubiquitous, they are differentially regulated by various stress signals. In addition, we have demonstrated that the calcium-channel blocker lanthanum chloride inhibited the induction of OsCam1 gene expression by both NaCl and mannitol treatments. These results suggest that osmotic stress induced expression of OsCam1 gene requires the $[Ca^{2+}]_{cyt}$ elevation that is known to occur in response to these stimuli.

CAD/CAM on factory automation (II) (F.A에 있어서의 CAD/CAM (II))

  • Lee, Bong-Jin
    • Journal of the Korean Society for Precision Engineering
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    • v.4 no.1
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    • pp.21-26
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    • 1987
  • CAD/CAM Software는 operating system(OS), 도형표시 회화처리, 형상정의, 형상조작, DBMS, 응용등으로 되어 있다. 그림 9에 이들 관계를 표시하고 있다. (1) operating system(OS) OS는 특히 CAD/CAM 특유의 것은 없고, 컴퓨터를 보다 효율있게 이용하기 위한 기본적인 software이다. OS 기능으로는 이하와 같은 것이 있다. .task 관리: CAD/CAM시스템은 일반적으로 복수의 단말에서 부터 다른 응용을 동시에 처리, 또는 back ground 로서 ploter를 움직인다. 이와같은 동질처리 (multitash)의 관리를 행한다. . Data 관리: 외부의 기억장치와의 data 수수를 행하기 위한 기본 software. .통신제어: 타의 컴퓨터와 통신회선으로 연결되어 있을 때, 이들의 data 수수를 행한다. 특히, LAN의 1 node로서 CAD/CAM 시스템이 연결되는 일이 많다. 이 경우, 다른 단말과의 communication 기능이 중요하다. .기타: FORTRAN등의 언어 processor, program 또는 data 관리와 같은 utilities가 포함되어 있다.

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Structure and expression analysis of the OsCam1-1 calmodulin gene from Oryza sativa L.

  • Phean-o-pas, Srivilai;Limpaseni, Tipaporn;Buaboocha, Teerapong
    • BMB Reports
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    • v.41 no.11
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    • pp.771-777
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    • 2008
  • Calmodulin (CaM) proteins, members of the EF-hand family of $Ca^{2+}$-binding proteins, represent important relays in plant calcium signals. Here, OsCam1-1 was isolated by PCR amplification from the rice genome. The gene contains an ORF of 450 base pairs with a single intron at the same position found in other plant Cam genes. A promoter region with a TATA box at position-26 was predicted and fused to a gus reporter gene, and this construct was used to produce transgenic rice by Agrobacterium-mediated transformation. GUS activity was observed in all organs examined and throughout tissues in cross-sections, but activity was strongest in the vascular bundles of leaves and the vascular cylinders of roots. To examine the properties of OsCaM1-1, the encoding cDNA was expressed in Escherichia coli. The electrophoretic mobility shift when incubated with $Ca^{2+}$ indicates that recombinant OsCaM1-1 is a functional $Ca^{2+}$-binding protein. In addition, OsCaM1-1 bound the CaMKII target peptide confirming its likely functionality as a calmodulin.

MIRACLEstation 20

  • 이문한
    • Computational Structural Engineering
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    • v.4 no.3
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    • pp.51-54
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    • 1991
  • 금성사는 뉴미디어 서비스, 컴퓨터와 종합정보 통신망의 결합 등 2000년대 신시장에 대한 적극적인 도전과 국내시장 개방에 대한 대처는 물론 주요 고기능 시스템을 운영할 수 있는 첨단 소프트웨어의 자체개발력 구축을 통한 국제 경쟁력을 강화하기 위하여 국산 워크스테이션을 개발하게 되었다. 썬사의 스팍스테이션 1과 100% 호환성을 갖는 MIRACLEstation 20은 스팍 RISC칩을 장착했고 운영체제는 썬 OS4.1, 또한 스팍스테이션에서 운용되는 모든 소프트웨어를 수정없이 사용할 수 있어 CAD/CAM/CAE, 금융, Software Engineering, Simulation, 국방, GIS 등의 분야에 3,300여종의 소프트웨어가 지원되는 워크스테이션이다. 이 제품은 207MB 하드디스크 1개와 1.44MB 플로피디스크 1개를 내장했고, 이더넷, TCP/IP, ONC/NFS를 기본 사양으로 LAN 및 WAN 환경에서 동기종 뿐만 아니라 타기종과 인터페이스를 용이하게 함으로써 워크스테이션의 필수요건인 네트워킹을 완벽하게 구현한다. 특히 국내 최초의 양산에 성공한 썬 클론 제품으로서 가격대비 성능면에서 뛰어난 제품이다.

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A Study on u-CCTV Fire Prevention System Development of System and Fire Judgement (u-CCTV 화재 감시 시스템 개발을 위한 시스템 및 화재 판별 기술 연구)

  • Kim, Young-Hyuk;Lim, Il-Kwon;Li, Qigui;Park, So-A;Kim, Myung-Jin;Lee, Jae-Kwang
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2010.05a
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    • pp.463-466
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    • 2010
  • In this paper, CCTV based fire surveillance system should aim to development. Advantages and Disadvantages analyzed of Existing sensor-based fire surveillance system and video-based fire surveillance system. To national support U-City, U-Home, U-Campus, etc, spread the ubiquitous environment appropriate to fire surveillance system model and a fire judgement technology. For this study, Microsoft LifeCam VX-1000 using through the capturing images and analyzed for apple and tomato, Finally we used H.264. The client uses the Linux OS with ARM9 S3C2440 board was manufactured, the client's role is passed to the server to processed capturing image. Client and the server is basically a 1:1 video communications. So to multiple receive to video multicast support will be a specification. Is fire surveillance system designed for multiple video communication. Video data from the RGB format to YUV format and transfer and fire detection for Y value. Y value is know movement data. The red color of the fire is determined to detect and calculate the value of Y at the fire continues to detect the movement of flame.

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