• Title/Summary/Keyword: Original Shape

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RAPD Analysis on the Species of Pinelliae Tuber (RAPD 방법을 이용한 반하류 한약재의 감별 연구)

  • 배명효;김규열;정유헌;최호영
    • The Journal of Korean Medicine
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    • v.20 no.4
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    • pp.16-22
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    • 2000
  • This study intends to report the significance of several experimental results obtained from analysing the genes extracted from the plants and herbal medicine such as P. temalta (Thunb.) Breit, A. amurense var serratum Nakai, A. erubescens (Wall.) Schott, Pinelliae Tuber and Arisaematis Tuber, mainly by the method of RAPD(randomly amplified polymorphic DNA) and the method of RFLP(restriction fragment length polymorphism) on ITS(internal transcribed spacer) region. Genomic DNA could be extracted from both original plants and dried materials. DNA fragments of P. temata kind and A. amurense kind showed the same aspect separately within the same species under the method of RAPD using random primer, while various aspects(polymorphism) were discovered among different species. In RAPD analysis by uniprimer, common bands were extracted from all types of P. temata in the case of uniprimer #4, which were distinguished from the kind of A. amurense. Other polymorphic bands appeared in between different A. amurense species as well. In the case of uniprimer #11, particular band came out in the kind of P. temata. On the other hand, in the case of uniprimer #5, #6, and #8, various bands(polymorhism) were revealed in both kinds of P. temata and A. amurense. Although further study is needed to ascertain whether these results are due to the differences of species, kinds, or growing place, the results could be used as a scientific method of identifying the substitutes for A. amurense genus. The author believes that as if P. temata class of plants used in this experiment are different among themselves in terms of the shape, size and property, those are clearly a class of P. temata or belong to the same genus.

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Conservation and Analysis of Inner Materials of a Stoneware Bottle from Incheon Ongjingun Yeongheungdo Shipwreck (인천 옹진군 영흥도선 출수 도기병의 보존처리와 내용물 분석)

  • Kim, Hyoyun;Kim, Seojin
    • Journal of Conservation Science
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    • v.32 no.4
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    • pp.449-457
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    • 2016
  • A stoneware bottle was recovered from the Incheon Yeongheungdo Shipwreck in 2013. Primary observations showed that this object had a sealed opening and was covered with a foreign material. After it was cleaned via mechanical and chemical methods, wave patterns were observed on the outer surface of the bottle and a yellowish-brown transparent material was found within. In this paper, the process of conserving the stoneware bottle and the analysis conducted on the unknown material found within is explained. The conservation process included steps such as cleaning, desalination and restoration. After of the missing area located at the rim, the original shape of the bottle was made clear. In addition, the unknown inner material was analyzed using FT-IR and GC-MS. Results showed that the material is similar to golden lacquer. It is speculated that the Yeongheungdo Ship had wrecked during the Unified Silla period while carrying a bottle loaded with golden lacquer.

Improved Methodology for Identification of Cryptomonads: Combining Light Microscopy and PCR Amplification

  • Xia, Shuang;Cheng, Yingyin;Zhu, Huan;Liu, Guoxiang;Hu, Zhengyu
    • Journal of Microbiology and Biotechnology
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    • v.23 no.3
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    • pp.289-296
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    • 2013
  • Cryptomonads are unicellular, biflagellate algae. Generally, cryptomonad cells cannot be preserved well because of their fragile nature, and an improved methodology should be developed to identify cryptomonads from natural habitats. In this study, we tried using several cytological fixatives, including glutaraldehyde, formaldehyde, and their combinations to preserve field samples collected from various waters, and the currently used fixative, Lugol's solution was tested for comparison. Results showed that among the fixatives tested, glutaraldehyde preserved the samples best, and the optimal concentration of glutaraldehyde was 2%. The cell morphology was well preserved by glutaraldehyde. Cells kept their original color, volume, and shape, and important taxonomic features such as furrow/gullet complex, ejectosomes, as well as flagella could be observed clearly, whereas these organelles frequently disappeared in Lugol's solution preserved samples. The osmotic adjustments and buffers tested could not preserve cell density significantly higher. Statistical calculation showed the cell density in the samples preserved by 2% glutaraldehyde remained stable after 43 days of the fixation procedure. In addition, DNA was extracted from glutaraldehyde preserved samples by grinding with liquid nitrogen and the 18S rDNA sequence was amplified by PCR. The sequence was virtually identical to the reference sequence, and phylogenetic analyses showed very close relationship between it and sequences from the same organism. To sum up, the present study demonstrated that 2% unbuffered glutaraldehyde, without osmotic adjustments, can preserve cryptomonads cells for identification, in terms of both light microscopy and phylogenetic analyses based on DNA sequences.

A Nonuniform Sampling Technique and Its Application to Speech Coding (비균등 표본화 기법과 음성 부호화로의 응용)

  • Iem, Byeong-Gwan
    • Journal of the Korean Institute of Intelligent Systems
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    • v.24 no.1
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    • pp.28-32
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    • 2014
  • For a signal such as speech showing piece-wise linear shape in a very short time period, a nonuniform sampling method based on the inflection point detection (IPD) is proposed to reduce data rate. The method exploits the geometrical characteristics of signal further than the existing local maxima/minima detection (MMD) based sampling method. As results, the reconstructed signal by the interpolation of the IPD based sampled data resembles the original speech more. Computer simulation shows that the proposed IPD based method produces about 9~23 dB improvement over the existing MMD method. To show the usefulness of the IPD technique, it is applied to speech coding, and compared to the continuously variable slope delta modulation (CVSD). The nonuniformly sampled data is binary coded with one bit flag set "1". Noninflection samples are not sent, but only flag bits set 0 are sent. The method shows 0.3 ~ 9 dB SNR and 0.5 ~ 1.3 mean opinion score (MOS) improvements over the CVSD.

A Study on the Characteristics of Building the Japanese Castle at the Period of Japanese Invasion of Korea in 1592 and in 1597 (임진왜란과 정유재란시기 왜성 축조방법에 대한 연구)

  • Lee, Hyung-Jae
    • Journal of architectural history
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    • v.18 no.1
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    • pp.31-49
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    • 2009
  • Periodically, the Japanese Castle was created in the domestic of Japan and then 2 Invasions into Chosun was started. The Japanese Castle in the domestic of Japan was repaired several times by the building boom of castle before & after 2 invasions and so the initially-built type of castles was changed. Accordingly, there are much difficulties to understand the original shape of Japanese Castle. Through the Japanese Castle within Korea called as the fossil of Japanese Castle, I would like to examine & consider its building period and characteristics. The terminology called as [Two Side Stone's Wall] is that of castle which is not acknowledged in the Japanese Academic Circles. However, it means the two-fold wall of Japanese Castle which was widely applied to the fortification way in the Age of Japan Edo. The terminology of [Sori] says the stonework curve in the corner of Japanese Castle which is indicated best in the Japanese Castle. It calls the curve as like the fan frame. [Curb Stone's Wall] says the type of castle wall constructed with over 1 face in wall body of Japanese Castle. (1) About classifying the construction period of Japanese Castle, the curb stone's wall and the castle having no two side stone's wall must consider the building period as that of Japanese Invasion of Korea in 1592. If there was [Sori], the two-side stone's wall was used and the place which supported the documentary data, in particular, the place having the record of contraction is considered to be confirmed as the castle constructed in the period of Japanese Invasion of Korea in 1597. (2) The two-side-type stone's wall shown in the Modern Japanese Stone Castle is difficultly considered to be generated from the Japanese Castle at the period of Japanese Invasion of Korea in 1592 and in 1597. (3) The beautiful [Sori] shown in the Modern Japanese Stone Castle was started from the Japanese Castle of Korea at the period of Japanese Invasion of Korea in 1597. It is difficultly considered which its indication was firstly generated by the Chaesung-Folded Segment Structure.

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Comparison of Time-Domain Imaging Algorithms for Ultra-Wideband Radar with One-Dimensional Synthetic Aperture (1차원 합성 개구면을 가진 초광대역 레이더의 시영역 기반 영상화 기법 비교)

  • Kim, Dae-Man;Hong, Jin-Young;Kim, Kang-Wook
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.10
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    • pp.1175-1184
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    • 2008
  • Delay-sum back projection(DSBP) algorithm and the time reversal algorithm based on the finite-difference time-domain method are compared. The two algorithms, which operate in the time domain, can process the ultra-wideband (UWB) radar data to generate images that are close to the original location and shape of the target. For the experiment, the UWB radar consists of a network analyzer, a resistive V dipole antenna, a scanner, and a control computer. The radar aperture is synthesized by linearly scanning the antenna. A calibration procedure is applied to the measured data to remove signal distortion and clutter. The two algorithms are applied to the same data on the same platform. It is shown that the DSBP algorithm produces better images but takes longer time to produce the images than the FDTD-TR algorithm.

Formulation of Generalized Hoek-Brown Model and Development of Rounded Hoek-Brown Model (일반화된 Hoek-Brown 모델의 정식화 및 Rounded Hoek-Brown 모델의 개발)

  • Kim Bum-Sang;Kwon O-Soon;Jang In-Sung
    • Journal of the Korean Geotechnical Society
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    • v.21 no.8
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    • pp.37-43
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    • 2005
  • Hoek-Brown model, which was developed in order to predict the behavior of rock mass, has widely been utilized and revised by many researchers to solve various problems encountered in tunnelling and slope stability analysis. However, there is no schematic investigation on the application of the Hoek-Brown model to numerical analysis including finite element simulations. In this paper the Hoek-Brown model was formulated as a constitutive model according to the procedure of generalized plasticity theory, and a Rounded Hoek-Brown model, which could overcome the numerical difficulties by modifying the edge part of the yield surface as a curve shape, was newly proposed. The new model could satisfy the requirements as an elasto-plastic constitutive soil model and follow the yield surface of the original Hoek-Brown model in the compression mode. The constitutive equation for the proposed model herein was established and presented to be applicable to the generalized nonlinear finite element analysis.

Interactive G$^1$ Splines with Tangent Specification Method (접선 지정법을 이용한 대화형 G$^1$스플라인)

  • Ju, U-Seok;Park, Gyeong-Hui;Lee, Hui-Seung
    • The Transactions of the Korea Information Processing Society
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    • v.1 no.4
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    • pp.531-540
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    • 1994
  • Spline curve scheme is the most valuable tool for the CAD of industrial products. Hence, the development of a new, effective curve scheme can have immediate impact on the current design industries. This paper develops and implements a new methodology for the implementation of the visually continuous class of splines which can produce a more flexible and diverse curve shapes. This class of splines has advantaged over existing splines in that it can accommodate wider range of shapes while maintaining the interpolators property of the ordinary cardinal splines. Most importantly, we avoid using the previous method of implementing G$^1$ curves, where users must specify scalar values for the control of curve shapes. We derive and implement an easy-to -use transformation between the user-specified graphical tangent vectors and the actual parameters for the resulting curve. Since the resulting curve shape reflects original tangential direction faithfully, CAD users can simply represent approximate curve shapes with proper tangents. Consequently, a simple user interface device such as a mouse can effectively produce a various spline curves using the proposed spline tool.

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Multiscale Analysis on Vibration of the Photo Responsive Polymer (광변형 고분자의 동적 진동에 관한 멀티스케일 해석)

  • Yun, Jung-Hoon;Li, Chenzhe;Chung, Hayoung;Choi, Joonmyung;Cho, Maenghyo
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.29 no.6
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    • pp.571-575
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    • 2016
  • Photo responsive polymer(PRP) is well known for its photo deformation under UV light, and goes back to its original shape in visible light due to the photoisomerization of the azobenzene inside the PRP. In this paper, dynamic study of the vibration in PRP is discussed. In order to predict photo-deformation of the PRP a multiscale modeling is introduced which covers quantum level photo excitation, microscopic morphology, and macroscopic deformation of the PRP. A simple 1D beam model is introduced to model dynamic bending behavior of the PRP. Through fast Fourious transformation analysis, we identify that vibration frequency of the PRP can be controlled by light polarization angle.

The Analysis of Landscape Ecological Effect of Forest by Trail-Building (등산로 개설에 의한 산림의 경관생태학적 영향 분석)

  • Lee, Woo-Sung;Park, Kyung-Hun;Kim, Dong-Pil
    • Korean Journal of Environment and Ecology
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    • v.22 no.2
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    • pp.128-137
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    • 2008
  • The purpose of this study is to analyze the effect of hiking trails on forest landscape's fragmentation, based on which also to assess the landscape-ecology-based integrity by small drainage area by selecting Bukhansan National Park as a survey target. The results of effect analysis are as follow; size of forest patch decreased; the density of patch and edge increased; patch shape became complicated; the dimensions of core area noticeably decreased and proximity degree between patch increased after trail-building. In addotion, the assessment results of overall landscape ecology-based integrity by small drainage basin showed that the Bukhansan catchment area was highest making 3.7 point, while Gugi catchment area was rated the lowest making 1.6 point. Putting the above results together, it is necessary to prohibit the opening up of unnecessary trails and to make room for ecological restoration of damaged and disturbance area to their original state as nature goes for landscape-ecology-based conservation and management of forests.