• 대한본초학회지
• /
• 제23권2호
• /
• pp.1-8
• /
• 2008

Objectives : The purpose of this study was to evaluate on the antimicrobial effect on the periodontal pathogens and anti-inflammatory effect of Artemisiae Iwayomogii Herba. Artemisiae Iwayomogii Herba has been used for treating as Artemisiae Capilaris Herba in Korea. Methods : Artemisiae Iwayomogii Herba was prepared by extracting medicinal herb with water. We investigated antimicrobial activity by the minimun inhibitory concentration (MIC) test. We also investigated inhibition of IL-$1{\beta}$-induced collagenase-l(MMP-l), stromelysin-1(MMP-3), interleukin-6 gene expression in human gingival fibroblasts. Results : The antimicrobial effect of Artemisiae Iwayomogii Herba was evaluated with MIC against periodontopathogens; Porphyromonas gingivalis 2561, W50, A7A1-28, 9-14K-1, Prevotella intermedia28, and Actinobacillus actinomycetemcomitans Y4, MICs of Artemisiae Iwayomogii Herba were 0.156 mg/ml, 0.625 mg/ml, 0.313 mg/ml, 1.25 mg/ml, 10 mg/ml and 10 mg/ml. The anti-inflammatory effect of Artemisiae Iwayomogii Herba was evaluated with Influence of herbs on the IL-$1{\beta}$-induced expression of MMP-1, MMP-3, interleukin-6, IL-$1{\beta}$ increased MMP-1, MMP-3, interleukin-6 mRNA levels. Artemisiae Iwayomogii Herba significantly inhibited IL-$1{\beta}$-induced MMP-1, MMP-3, interleukin-6 gene expressions in a dose-dependent manner. Conclusions : These results suggested that Artemisiae Iwayomogii Herba might reduce the excessive proteolytic capacity of the gingival fibroblast during inflammation and could be developed a new drug in periodontitis.

• The Korean Journal of Physiology
• /
• 제15권1호
• /
• pp.9-18
• /
• 1981

For centuries, ginseng has been used for the therapeutic purpose in oriental herb medicine. Several studies have been conducted in the past to evaluate the effect of ginseng on erythropoiesis. However the results were controversial. We therefore attempted in the present studies to evaluate the effect of ginseng on the erythropoietic activity. In one series of experiments, the recovery pattern of peripheral blood(red cell count, hemoglobin content, hematocrit and reticulocyte count) was studied in posthemorrhagic anemic rabbits. After animals were maintained with normal(control group) or 1 gm% ginseng (experimental group) diet for 2 weeks, hemorrhagic anemia was induced by withdrawing blood equivalent to 25% of the total blood volume and then changes in peripheral blood were followed for following 30 days. In other series of experiments, we studied effect of ginseng on erythrokinetics using $^{59}Fe$. $^{59}Fe(10{\sim}40\;{\mu}Ci/animal)$ was injected intravenously after animals were fed with normal (control group) or 1 gm% ginseng(experimental group) diet for 2 weeks. And radioactivities in the blood compartments were measured at appropriate intervals for 15 days. Front these various erythrokinetic parameters were estimated. Results are summarized as follows: 1) Reticulocyte count was higher in the experimental group than in the control group after 2 weeks of administration of experimental diet. During the posthemorrhagic period, the reticulocyte count increased in both the control and experimental groups, but the increase appeared much earlier in the experimental group. 2) The posthemorrhagic recoveries of hematocrit, hemoglobin content and red cell count appeared to be faster in the experimental group as compaired with the control group. 3) The half life$(T_{1/2})$ of $^{59}Fe$ in the plasma was significantly(P<0.05) shorter in the experimental group(82.6 min, N=8) than in the control group(121 min, N=6). Plasma iron turnover (PIT) of the experimental group (1.78 mg/dl/24 hr.) was approximately 4 times greater than that of the control group(0.45 mg/dl/24 hr.). 4) The maximum red cell utilization(RC-U) was 82.1% in the experimental group ana 74.5% in the control group. Red cell iron turnover(RIT) of the experimental group(1.62 mg/dl/24 hr.) was slightly higher than that of the control group(0.35 mg/dl/24 hr). 5) Erythron turnover was significantly(p<0.05) greater in the experimental group(1.27 mg/dl/24 hr.) than in the control group(0.24 mg/dl/24 hr.). Marrow transit time of the experimental group(2.05 days) tended to he faster than that of the control group(2.84 days). These results suggest that the gingseng improves the recovery of posthemorrhagic anemia and stimulates the erythropoiesis in rabbits.

• 한방재활의학과학회지
• /
• 제24권3호
• /
• pp.131-137
• /
• 2014

Objectives The purpose of this study was to compare the effects of MSAT (Mostion style acuputure treatment) on piriformis muscle regarding leg radiating pain with HIVD of L-SPINE. Methods Forty patients who received inpatient treatment from Febuarary 1 to March 31, 2014 in the Daejon-Jaseng oriental medicine hospital were divided into 2 groups by blocked randomization : Group A (n=20)=routine treatment on HIVD of L-SPINE with MSAT on piriformis muscle and Group B (n=20)=routine treatment on HIVD of L-SPINE (acupuncture, Shinbaro pharmacopuncture, herb medication, chuna treatment and physiotherapy). The MSAT (Mostion style acuputure treatment) on piriformis muscle was administered each day and both groups were received routine treatment each day. Evaluations were made before treatment, after 3 days and 7 days, and before discharge using the NRS (numeric rating scale) and ODI (oswestry disability index). The statistically significance was evaluated by SPSS 18.0 for windows. Results Compared to before treatment, the NRS (numeric rating scale) and ODI (oswestry disability index) of both A and B groups after 3, 7 days and before discharge of treatment significantly decreased (p<0.05). Regarding group comparison, the NRS (numeric rating scale) and ODI (oswestry disability index) of A group was significantly lower than the B group at 3, 7 days, before dischanre (p<0.05). Regarding improvement of the NRS (numeric rating scale) and ODI (oswestry disability index), A group was significantly lower than the B group at 3 day (NRS), 7day (ODI) (p=0.000) but there was no significant difference between the two groups at 7 days (NRS) and before discharge (p>0.05). Conclusions Compared to just routine treatment, the MSAT (Mostion style acuputure treatment) on piriformis muscle significantly improved the NRS (numeric rating scale) and ODI (oswestry disability index) after 3, 7 days, and before discharge. Because early intervention determines the grade of Leg radiating pain with HIVD of L-SPINE, the MSAT (Mostion style acuputure treatment) on piriformisat the early stage will be clinically helpful to patients.

• 동의생리병리학회지
• /
• 제24권5호
• /
• pp.837-842
• /
• 2010

The purpose of this study is to develop bathing aids as a strategic products to promote the medical tourism in Sancheong Jirisan Oriental medicinal herbs special district using medicinal herbs produced in Sancheong province, and to verify the effect of the bathing aids in vitro. We investigated the cytotoxicity activity, antioxidant activity, antiaging and whitening effects of Sanchung-PNU 1 (SP1) and Sanchung-PNU 2 (SP2) made with traditional medicinal herbs. The cytotoxicity activity was measured by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Antioxidant activities were determined by DPPH (1.1-diphenyl-2-picrylhydrazyl) radical scavenging capacity assay. We measured the inhibitory effect against tyrosinase activity to prove the whitening effect, and the inhibitory effect against elastase activity to prove the anti-aging effect. Two proposed prescriptions, SP1 and SP2, showed not significant cytotoxicity but significant (p<0.001) improvement in anti-oxidation, anti-wrinkle, and whitening effects compared to the control group. The result shows that these bathing aids have excellent DPPH radical scavenging effect and significant inhibitory effect against elastase and tyrosinase activity. These findings suggest that these bathing aids have a strong antioxidant, anti-aging, and whitening effect.

• Biomolecules & Therapeutics
• /
• 제24권4호
• /
• pp.410-417
• /
• 2016

Quercetin is a flavonoid usually found in fruits and vegetables. Aside from its antioxidative effects, quercetin, like other flavonoids, has a various neuropharmacological actions. Quercetin-3-O-rhamnoside (Rham1), quercetin-3-O-rutinoside (Rutin), and quercetin-3-(2(G)-rhamnosylrutinoside (Rham2) are mono-, di-, and tri-glycosylated forms of quercetin, respectively. In a previous study, we showed that quercetin can enhance ${\alpha}7$ nicotinic acetylcholine receptor (${\alpha}7$ nAChR)-mediated ion currents. However, the role of the carbohydrates attached to quercetin in the regulation of ${\alpha}7$ nAChR channel activity has not been determined. In the present study, we investigated the effects of quercetin glycosides on the acetylcholine induced peak inward current ($I_{ACh}$) in Xenopus oocytes expressing the ${\alpha}7$ nAChR. $I_{ACh}$ was measured with a two-electrode voltage clamp technique. In oocytes injected with ${\alpha}7$ nAChR copy RNA, quercetin enhanced $I_{ACh}$, whereas quercetin glycosides inhibited $I_{ACh}$. Quercetin glycosides mediated an inhibition of $I_{ACh}$, which increased when they were pre-applied and the inhibitory effects were concentration dependent. The order of $I_{ACh}$ inhibition by quercetin glycosides was Rutin${\geq}$Rham1>Rham2. Quercetin glycosides-mediated $I_{ACh}$ enhancement was not affected by ACh concentration and appeared voltage-independent. Furthermore, quercetin-mediated $I_{ACh}$ inhibition can be attenuated when quercetin is co-applied with Rham1 and Rutin, indicating that quercetin glycosides could interfere with quercetin-mediated ${\alpha}7$ nAChR regulation and that the number of carbohydrates in the quercetin glycoside plays a key role in the interruption of quercetin action. These results show that quercetin and quercetin glycosides regulate the ${\alpha}7$ nAChR in a differential manner.

• 한국자원식물학회지
• /
• 제22권4호
• /
• pp.281-286
• /
• 2009

홍경천의 잎, 줄기, 뿌리 절편을 이용하여 기내 부정근의 생산 체계를 확립하였다. 먼저 홍경천의 잎, 줄기, 뿌리 절편을 0.1, 1.0, 3.0 및 5,0 mg/L의 IBA와 sucrose가 10, 30, 50 및 100 g/L가 첨가된 MS 배지위에 치상하여 부정근의 유도율을 조사하였다. 부정근의 유도는 잎, 줄기 절편에서 IBA의 농도가 5.0 mg/L 일때 가장 높은 유도율을 보였으며, 뿌리 절편은 IBA 3.0 mg/L 첨가된 배지에서 부정근 유도율이 가장 높았다. Sucrose의 농도는 30 g/L가 첨가되었을 때 잎, 줄기, 뿌리 절편에서 높은 유도율을 나타났다. 고체배지 조건에서 부정근의 유도율이 가장 우수한 조건을 기본으로 액체배양을 실시하였으며, 염의 농도에 따른 부정근의 증식조건을 조사하였다. 1/3MS 배지에서 홍경천의 부정근을 배양하였을 때 1/2MS, MS 액체 배지조건 보다 약 2배, 2.5배의 부정근 생장량을 보였다.

• Journal of Applied Biological Chemistry
• /
• 제61권1호
• /
• pp.75-78
• /
• 2018

식물생장호르몬인 에틸렌의 전구체인 에테폰은 기체상의 에틸렌을 대신하여 가장 빈번히 사용되는 농약 중 하나로, 식물 이차 대사산물의 생산에 사용되고 있다. 최근 콩잎으로부터 식물 이차 대사산물인 이소플라본 생산에 에틸렌 및 에테폰 처리에 관한 연구가 시도되었으나, 에테폰의 잔류안전성에 관한 연구가 보고되지 못하였다. 이에 본 연구는 콩잎 생산시 에테폰을 경엽과 관주 처리하여 나타나는 잔류 변화를 연구하였다. 경엽 처리시 콩잎 중 에테폰의 잔류반감기는 1회와 2회 처리시 26.6 h과 21.1 h으로 확인되었고, 처리 3일 후 콩잎 중 에테폰은 최대 $60mg\;kg^{-1}$이었으나, 콩잎 건조 후 에테폰은 불검출 되었다. 관주 처리시 20.1 h 후 최대 잔류농도가 관찰되었으나, 관주처리에 의한 Total phenol 함량 변화는 관찰되지 않았다.

• 대한한의학회지
• /
• 제29권2호
• /
• pp.182-192
• /
• 2008

Objective: The purpose of this study was to evaluate on the antimicrobial effect on the periodontal pathogens and anti-inflammatory effect of Eriobotryae folium. Eriobotryae folium are constituent herbs of Gagamgamroum, which has been used for a long time in oriental medicine as a herbal medicine for treating halitosis and toothache. Method: Eriobotryae folium was prepared by extracting medicinal herb with water. We investigated antimicrobial activity by the minimum inhibitory concentration (MIC) test. We also investigated inhibition of $IL-1{\beta}-induced$ collagenase (mmp-1), stromelysin-1 (mmp-3), interleukin-6 gene expression in human gingival fibroblasts using RTPCR analysis. Result: The antimicrobial effects of Eriobotryae folium was evaluated with MIC against periodontopathogens; Porphyromonas gingivalis 2561, W50, A7A1-28, 9-14K-1, Prevotella intermedia 28, and Actinobacillus actinomycetemcomitans Y4. MICs of Eriobotryae folium were 1.25 mg/ml, 2.5 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 10 mg/ml and 10 mg/ml. The anti-inflammatory effect of Eriobotryae folium was evaluated with influence of herbs on the $IL-1{\beta}-induced$ expression of mmp-1, mmp-3, and interleukin-6. $IL-1{\beta}$ increased mmp-1, mmp-3, and interleukin-6 mRNA levels. Eriobotryae folium significantly inhibited $IL-1{\beta}-induced$ mmp-1, mmp-3, and interleukin-6 gene expressions in a dose-dependent manner. Conclusion: These results suggested that Eriobotryae folium might reduce the excessive proteolytic capacity of the gingival fibroblast during inflammation and could be developed as a new drug for periodontitis.

• 식물분류학회지
• /
• 제42권4호
• /
• pp.260-266
• /
• 2012

깽깽이풀의 발생학적 연구가 수행되었으며, 이를 매자나무과내의 근연속 들과 비교연구하였다. 깽깽이풀의 발생학적 특징은 4포약, basic type의 약벽형성, 2핵성의 glandular tapetum, 약벽 표피세포의 숙존, 내피층이 섬유상조직으로 발달, 판개형의 약 열개, 화분모세포의 연속형 세포질분열, 정사면체형 화분사분체, 약 열개시 화분은 2세포성, 양주피성, 도생배주, 후층성주심, 한개의 포원세포, 마디풀형 배낭형성, 성숙배낭은 타원형, 핵형 배유형성, 배 발생은 Onagrad 형태, 종자는 종의가 있고, 종피는 외종피외층형 등의 특징을 나타내었다. 매자나무과내의 다른 속들과 비교한 결과 깽깽이풀속은 융단세포의 핵수, 감수분열시 세포질의 분열패턴, 그리고 외종피외층의 두께 등에서 매자나무속이나 Mahonia속 보다는 삼지구엽초속과 Vancouveria 속에 좀 더 닮았다. 또한, 약벽형성 패턴, 융단세포의 핵수, 주심cap의 형성, 반족세포의 행동패턴등에서 한계령풀속과도 유사한 형질이 있었다. 그럼에도 불구하고, 한계령풀속은 다른 많은 발생형질과 분자 data에서 깽깽이풀속과는 다르다. 따라서 발생학적 증거들은 깽깽이풀속이 삼지구엽초속(Epimedium)과 Vancouveria 속에 가장 가까운 유연관계라는 것을 제시하고 있다.

• 한국자원식물학회지
• /
• 제26권1호
• /
• pp.44-51
• /
• 2013

본 연구에서는 파극천 추출물의 수면연장 시간, 항 경련효과 및 시험관내에서 파극천의 각 분획별 활성산소의 소거능을 관찰하여 본 결과 pentobarbital을 투여한 대조군의 수면 유도시간 및 수면시간($3.77{\pm}0.56$분, 수면시간 $68.3{\pm}6.8$분)이 파극천 추출물(200 mg/kg)을 전 처리하고 pentobarbital을 투여함으로써 수면유도 시간($3.95{\pm}0.56$분) 및 수면시간($110.4{\pm}10.3$분)이 연장되었다. PTZ에 의해 유도되는 경련 발작이 대조군 $4.9{\pm}0.98$인데 비해 파극천(200 mg/kg)의 전처리로 경련박작이 $3.2{\pm}0.54$로 현저히 억제되었으며 이 결과는 PTZ의 경련발현시간, 경련유지시간 및 사망시간에 미치는 파극천의 영향과 유사하였다. 뇌중 GABA 함량은 PTZ의 투여로 정상군에 비하여 억제되던 것이 파극천의 전처리로 증가 되었으며, GABA-T의 활성과 지질과산화의 함량은 PTZ의 투여로 각각 증가되던 것이 파극천 추출물의 투여로 억제되었다. 시험관내에서 PTZ로 유도된 뇌조직중의 hydroxy radical의 생성량은 butanol 및 dichlomethane 분획의 첨가($20{\times}10^{-2}g/ml$)에서 약 35%에서 50%까지 감소하였다.