• 한국환경복원기술학회지
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• 제14권1호
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• pp.43-55
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• 2011

This study was carried out to analyze vegetation, soil characteristic, and ordination of the Young-bong region in Woraksan National Park. Communities by species in Woraksan forest were composed of Quercus mongolica community group. This community group was classified into Pinus densiflora community, Quercus variabilis community, Carpinus cordata community and Quercus mongolica community. In the study, it was found that the composition of organic matter was 9.6~19.6%, nitrogen (0.3~0.7%), available phosphorous (4.5~8.8mg/kg), exchangeable K ($0.3{\sim}0.5cmol^+/kg$), exchangeable Ca ($1.6{\sim}6.75cmol^+/kg$), exchangeable Mg ($0.8{\sim}1.55cmol^+/kg$), cation exchange capacity (C.E.C.; $7.6{\sim}32.95cmol^+/kg$), and pH was 4.5~5.1 respectively. The Carpinus cordata community were mostly found in high elevation and high percentage of total nitrogen, C.E.C., and organic matter. Controversially, Pinus densiflora community and Quercus variabilis community were found mostly in low elevation and low percentage of total nitrogen, C.E.C., and organic matter. The Quercus mongolica community were found in medium elevation and medium percentage of total nitrogen, C.E.C., and organic matter.

• 한국축산시설환경학회지
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• 제3권1호
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• pp.13-18
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• 1997

This study was conducted to investigate the physicochemical changes during the aerobic composting of swine manure mixed with bulking agent, sawdust(v/v, 1:1), in a full-scale composting plant using rectangular escalator-aginated bed composting system. Physical and chemical properties were analyzed on the samples which were collected at 5, 15, and 25 day of composting, curing and final step. The results of this study were summarized as follows; 1. Moisture and K2O content, and pH of final step were higher than those of 5th day of composting (p＜0.05). 2. Ammonium nitrogen, total organic corbon and organic matter content, and electrical conductivity(EC) were significantly decreased (p＜0.05) but nitrate nitrogen, ash and P2O5 content increased(p＜0.05) throughout the aerobic composting process. 3. Total organic carbon per total nitrogen(C/N) and total organic matter per total nitrogen(OM/N) ratio were significantly decreased throughout the aerobic composting process(p＜0.05). 4. Physical and chemical properties of swine manure were varied by aerobic fermentation using rectangular escalor-aginated bed composting system.

• 한국유기농업학회지
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• 제23권4호
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• pp.867-873
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• 2015

유기자재의 토양 중 질소 무기화 특성을 구명하기 위해 유박(CF-I, CF-II), 아미노산(AAF-I, AAF-II)을 각각 처리하여 28주간 항온시험을 실시하였다. 항온기간 동안 누적 질소 무기화량을 1차 반응 속도식(first-order kinetics)에 적용하여 잠재적 질소무기화량($N_0$)를 평가 한 결과 AAF-II에서 27.71 N mg/100g로 가장 높았으며, CF-I에서 21.69 N mg/100g로 가장 낮았다. 그리고 잠재적 순질소무기화량($N_0$ treatment - $N_0$ control)은 CF-I, CF-II, AAF-I, AAF-II 처리에서 각각 2.55, 5.83, 3.66, 8.57 N mg/100g으로 나타났으며, 28주 동안 실제 질소무기 화량의 97.3-112.9%에 해당되었다. 특히 유박, 아미노산을 처리한 토양의 유기태 질소의 무기화 반감기($t_{1/2}$)는 17-21일로 유박과 아미노산 비료에 포함된 질소는 3주 이내에 무기화되는 것을 확인하였다. 따라서 유기농업에 이용되는 유박과 아미노산에 함유된 질소의 1/2는 3주 이내에 모두 무기화되는 것으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제12권2호
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• pp.273-278
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• 2002

Two-step fed-batch fermentations were carried out to overproduce Bacillus licheniformis maltogenic amylase (BLMA) in recombinant Escherichia coli. The first step was to increase the cell mass by controlling the feeding of a glucose solution, while the second step was designed to improve the amylase expression efficiency by supplementing organic nitrogen sources. The linear gradient feeding method was successfully adopted to maintain the glucose concentration below 0.2 g/l during the fed-batch mode, as effectively minimizing acetic acid formation. When the dissolved oxygen (DO) level became limiting, an accumulation of acetic acid and drastic decrease in specific BLMA productivity were observed. Glucose and organic nitrogen sources consisting of yeast extract and casein hydrolysate were simultaneously supplied in the pH-stat mode to further increase the specific BLMA expression efficiency. An organic nitrogen source consisting of 200 g/1 yeast extract and 100 g/1 casein hydrolysate was found to be the best among the various combinations tested. The feeding of an organic nitrogen source in the second-step fed-batch period was highly beneficial in enhancing the BLMA production. The optimized two-step fed-batch culture resulted in 78 g/l maximum dry cell mass and 443 U/ml maximum BLMA activity, corresponding to 1.5-fold increase in the dry cell mass and 3.7-fold enhancement in BLMA production, compared with the simple fed-batch fermentation.

• 한국물환경학회지
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• 제31권5호
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• pp.556-562
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• 2015

In order to remove the organic substances and the nitrate-nitrogen contained in wastewater, some researchers have studied the simultaneous removal of organics and nitrogen by using different biocathode microbial fuel cells (MFCs). The operating conditions for removing the contaminants in the MFCs are the external resistances, HRTs, the concentration of the influent wastewater, and other factors. This study aimed to determine the effect of the external resistors and organic loading rates, from the changing HRT, on the removal of the organics and nitrogen and on the production of electric power using the Denitrification Biocathode - Microbial Fuel Cell (DNB-MFC). As regards the results of the study, the removal efficiencies of $SCOD_{Cr}$ did not show any difference, but the nitrate-nitrogen removal efficiencies were increased by decreasing the external resistance. The maximum denitrification rate achieved was $129.2{\pm}13.54g\;NO_3{^-}-N/m^3/d$ in the external resistance $1{\Omega}$, and the maximum power density was $3,279mW/m^3$ in $10{\Omega}$. When the DNB-MFC was operated with increasing influent organic and nitrate loading by reducing the HRTs, the $NO_3{^-}-N$ removal efficiencies were increased linearly, and the maximum nitrate removal rate was $1,586g\;NO^3{^-}-N/m^3/d$ at HRT 0.6 h.

• Journal of Microbiology and Biotechnology
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• 제13권2호
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• pp.256-262
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• 2003

To enhance the production of heavy chain immunoglobulin G (HC IgG) in the suspension cultures of transgenic tobacco cells (Nicotiana tabacum), the effects of adding various cytokinins (i.e., growth regulators) and organic nitrogen sources to culture media were investigated. Four different cytokinins including kinetin, isopentenyladenine (IPA), 6-benzylaminepurine (BA), and zeatin were tested with or without dichlorophenoxyacetic acid (2,4-D), which is a typical growth regulator supplemented in the standard Murashige and Skoog (MS) medium. The productivity of intracellular HC IgG was increased by 36 and $42\%$, compared to the control, especially when IPA (2 mg/l) or BA (0.2 mg/l) was added to the media in the presence of 2,4-D, respectively. In the study of organic nitrogen sources, addition of each casein hydrolysate and tryptone to the culture media at a final concentration of 0.01 and 1 g/l, respectively. increased the productivity or he IgG as much as 68 and $67\%$, respectively, in comparison with the control, which was is MS medium without supplementation of any organic nitrogen sources. This study shows that the optimization of media composition could offer significant improvements in the production of foreign proteins in the suspension cultures of transgenic plants.

• Ocean and Polar Research
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• 제45권3호
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• pp.103-111
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• 2023

To evaluate the impact of effluents from land-based fish farms on the coastal ocean of Wando, Korea, we analyzed inorganic nutrients, particulate organic carbon (POC), dissolved organic carbon (DOC), and colored dissolved organic matter (CDOM) in the effluent and influent of land-based fish farms during the summer (July) of 2021. The average concentrations of nutrients (Dissolved inorganic nitrogen, phosphorus, and silicate; DIN, DIP, and DSi, respectively) in the effluents of this study area were 17±3.7 μM, 1.4±0.7 μM, and 14±1.6 μM, respectively. The average concentrations of POC and DOC were 37±22 μM and 81±13 μM, respectively, with POC accounting for about 30% for total organic carbon in effluents. The Reduced Dissolved Inorganic Nitrogen/Total Dissolved Inorganic Nitrogen ratio (0.7), potential short-period index, indicates that the discharge of nutrients excreted by the fish and unconsumed feed into coastal water results in such nutrients being deposited and accumulated in the sediment. Subsequently, this continuous accumulation triggers the release of ammonium ions during organic matter decomposition, and the ammonium-enriched waters that encroach on fish farms as influent seem to be due to the diffusion of high concentrations of ammonium from bottom sediment. Furthermore, we used fluorescence indices to examine the characteristics of organic matter sources, obtaining mean values of 1.54±0.19, 1.06±0.06, and 1.56±0.06 for the humification index, biological index, and fluorescence index, respectively, in the effluent. These results indicate that the organic matters had an autochthonous origin that resulted from microbial decomposition, and such organic matters were rapidly generated and removed by biological activity, likely supplied from the sediment. Our results suggest that the effluent from land-based fish farms could be a potential source of deoxygenation occurrence in coastal areas.

• 한국유기농업학회지
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• 제9권3호
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• pp.45-71
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• 2001

The duck-rice forming system is increasingly spread up throughout Korea since 1992. It is discussed the rice field, rice quality, weed and pest management in the duck-rice weeding system compared to conventional farming system. Moreover the optimizing duck population, system management and fertilizer application rate were reported. Energy input and output by duck-rice farming system were carefully compared with those of low input sustainable paddy field and conventional farming paddy field. To find out the environmentally sound function of duck-rice system, the total nitrogen in paddy soil and paddy water, and nitrogen cycle in paddy rice cultivation system were analysed. finally the input and output were calculated, and ecological characteristic were determined in terms of nitrogen balance, labor input, animal input, renewable energy input, turnover of soil organic matter, energy loss, non-renewable indirect and direct energy input. It was concluded duck-rice weeding system could be recommended in terms of net only environmentally sound, but also farmer's income. But there are still some research needs for successful adaption of duck-rice farming to investigate to determine the optimal population of duck in rice paddy field unit, release time of duckling, duck management after release, and strategy for duck marketing and duck processing.

• 한국환경과학회지
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• 제28권9호
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• pp.719-727
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• 2019

In this study, the effect on the stability of Aerobic Granular Sludge (AGS) with different Carbon/Nitrogen (C/N) ratios was investigated. The C/N ratios were controlled to 10.0, 7.5, 5.0, and 2.5 using the sequencing batch reactor, and the results showed that the removal efficiency of organic matter and total nitrogen decreased simultaneously with the decrease of C/N ratio. The removal efficiency of organic matter and total nitrogen at C/N ratio of 2.5 was 70.7% and 52.3% respectively. In addition, the AGS/mixed liquor suspended solids (MLSS) ratio showed a tendency to decrease from 85.7% to 73.7%, while the sludge volume index showed a tendency to increase from 82 mL/g to 102 mL/g as the C/N ratio decreased. At the same time, the apparent deviation of polysaccharide (PS) content in extracellular polymeric substances was observed, and polysaccharides/protein (PS/PN) ratio decreased from 0.62 to 0.31 as the C/N ratio decreased. Optical microscope observations showed that the reduction in C/N ratio caused the growth of filamentous bacteria and significantly affected the stability of AGS.

• 한국균학회지
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• 제44권3호
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• pp.166-170
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• 2016

Ectomycorrhizal fungi are associated with plants roots and acquire significant amounts of nitrogen sources from the soil. For artificial cultivation, mass production of ectomycorrhizal fungi in liquid media is required. We studied the edible ectomycorrhizal mushrooms Hygrophorus russula, Ramaria fumigata, Sarcodon aspratus, and Tricholoma matsutake. All strains except S. aspratus NIFoS 2031 grew generally well on modified Melin-Norkran's (MMN) medium compared to on other media. All strains analyzed in this study showed significantly higher growth on organic nitrogen. Specifically, two strains of H. russula significantly responded to both tryptone and neopeptone media. Among different species and strains, there were clear differences in the capacity to grow on animal-based organic nitrogen sources.