• Title/Summary/Keyword: Organic media

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Seasonal Weight in Seeding Mixture for the Restoration and Revegetation of the Disturbed Slopes (훼손 비탈면의 생태복원녹화를 위한 종자배합량의 계절별 가중치에 관한 연구)

  • Hur, Young-Jin;Ahn, Tae-Young
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.9 no.1
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    • pp.41-54
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    • 2006
  • In case of leaving artificial slopes resulting from large-scale constructions, there may be secondary damage caused by soil loss due to erosion and collapse. Furthermore, slope-restoring constructions have a few problems such as monotonous landscape and difficult succession of secondary vegetation due to reckless use of exotic grass, despite attaining the initial purpose of revegetation. To settle this problem, selected plants deemed to be proper for revegetation were used on one of thin vegetation base methods, CODRA SYSTEM, and made seeding mixture experimental plots considering germination rates differing in each season. Native herbs, native shrubs and exotic herbs contents were increased by 30% and 50% respectively, centered on seeding quantity(30g/$m^2$) used as design standard in the seed spray method, in order to figure out proper quantity for revegetation in each season.

Preparation and Characterizations of C60/Polystyrene Composite Particle Containing Pristine C60 Clusters

  • Kim, Jung-Woon;Kim, Kun-Ji;Park, Soo-Yeon;Jeong, Kwang-Un;Lee, Myong-Hoon
    • Bulletin of the Korean Chemical Society
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    • v.33 no.9
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    • pp.2966-2970
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    • 2012
  • Fullerene/polystyrene ($C_{60}$/PS) nano particle was prepared by using emulsion polymerization. Styrene and fullerene were emulsified in aqueous media in the presence of poly(N-vinyl pyridine) as an emulsion stabilizer, and polymerization was initiated by water soluble radical initiator, potassium persulfate. The obtained nano particles have an average diameter in the range of 400-500 nm. The fullerene contents in the nano particle can be controlled up to 15 wt % by varying the feed ratio, which was confirmed by themogravimetric analysis (TGA) and elemental analysis (EA). The structure and morphologies of the $C_{60}$/PS nano particles were examined by various analytical techniques such as dynamic light scattering (DLS), scanning electron microscope (SEM), transmission electron microscope (TEM), electron diffraction (ED) pattern, X-ray powder diffraction (XRD), and UV spectroscopy. Unlike conventional $C_{60}$/PS particles initiated by organic free radical initiators, in which the fullerene is copolymerized forming a covalent bond with styrene monomer, the prepared $C_{60}$/PS nano particles contain pristine fullerene as secondary particles homogeneously distributed in the polystyrene matrix.

The adsorption efficiency of ceramic filter media prepared with the steel-making slag for the removal of VOCs (휘발성 유기화합물 제거를 위한 제강슬래그로 제조된 세라믹 여재의 흡착효율)

  • Sin, Jun-Ho;Kim, Tae-Heui;Park, Kyung-Bong
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.20 no.3
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    • pp.153-157
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    • 2010
  • We studied the adsorption efficiency of steelmaking slag in removing volatile organic compounds (VOCs) for increasing the recycling rate of steel-making slag. Ceramic filter was prepared by mixing the steel-making slag and the diatomite which is used as adsorbents due to the advantage of the high specific surface area and regular mesopores. The adsorption efficiency for VOCs removal was about 80%, 96% and 85% in acetaldehyde, formaldehyde and ammonia, respectively. The adsorption efficiency over 80% for all The gases showed the practical possibility as the adsorption filter.

Streptomyces griseoplanus SL20209에 의한 Aminopeptidase M 저해제의 생산 조건

  • Ko, Hack-Ryong;Chun, Hyo-Kon;Sung, Nack-Kie;Kho, Yung-Hee
    • Microbiology and Biotechnology Letters
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    • v.24 no.3
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    • pp.336-343
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    • 1996
  • Maximum amount of the aminopeptidase M inhibitors produced by Streptomyces griseoplanus SL20209 in 500 ml-Erlenmeyer flask was accumulated after cultivation for 3 days at 28$\circ$C, thereafter the amount of inhibitors decreased slowly with a pH change to alkaline. Arabinose, xylose, mannose and soluble starch were good carbon sources for the production of the inhibitors. On the other hand, glucose was only good for the cell growth but potently inhibited the production of inhibitors. Natural organic nitrogen sources such as soybean meal, fish meal, gluten meal and peanut powder were good for the production of inhibitors, however, soytone, peptone and inorganic nitrogens such as NH$_{4}$C1 and NH$_{4}$NO$_{3}$ were poor. Inclusion of yeast extract (0.5%, w/v) or K$_{2}$HPO$_{4}$ (0.05%) into the production medium increased the production of inhibitors by accelerating cell growth. The production of inhibitors was slightly increased on the medium containing CaCO$_{3}$ (0.3%) and zeolite (0.5%), respectively. Optimal temperature and initial pH range for the production ot inhibitors were determined to be 28$\circ$C and 6.0-7.0, respectively. Employing two improved production media consisting of 3% arabinose or soluble starch, 2.5% soybean meal, 0.5% yeast extract, 0.05% K$_{2}$HP0$_{4}$, 0.1% CaCO$_{3}$ and 0.3% zeolite (pH 6.8), 1.8-fold increase in the amount of inhibitors was achieved, comparing with the basal medium used.

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Crystallization of a-Amylase and Protease of Aspergillus oryzae from Columm Chromatography (I) (칼럼크로마토그라피에 의한 아스퍼질러스 계통의 .alpha.-아미라제 및 프로테아제의 결정화 1)

  • 서항원
    • Korean Journal of Microbiology
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    • v.9 no.4
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    • pp.163-168
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    • 1971
  • Neutral protease which was obtained from a genus of Aspergilli as the crystal form were investigated for their purification and properties. The results of biochemical and enzymatic studies for their purification and properties in this enzyme were as follows. 1) On the wheat media containing 70%-water and $CaCo_{3}$, Aspergilus oryzae S.H.W. 131 is satisfactorily grown under the basic optimum conditions temperature $27^{\circ}C$- $30^{\circ}C$at relative humidity 100% for three days. 2) The enzyme solution extracted with water is successively purified through the passing on column of Asmti-177N for decolorization of it. And ion exchanger such as DEAAE Sphadex A-50 or Shepadex G-100 and fraction collector is necessary for the sepearte treatments of this enzyme. After washing it with organic solvents as aceton-EtOH, etc., it should be dried on the vacuum dryer at $40^{\circ}C$) The protease activity is determined by the amounts of amino acids, tyrosine. 4) The optimum pH of neutral protease is 6.0-8.0. 5) In effectively decomposing with this neutral protease, the optimum temperature is $35^{\circ}C$. 6) It is interesting that the amounts of metal ion affects the activity of neutral protease. For examples, if it were treated with manganic ion, its activity would be more effective than any other that.

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Gas Chromatographic Profiling for the Screening of Candida tropicalis Mutant Producing Tridecanedioic Acid (Gas Chromatographic Profiling법을 이용한 Tridecanedioic Acid를 생산해내는 Candida tropicalis Mutant의 탐색연구)

  • Kim, Jung-Han;Lee, Sang-Jun;Park, Hyoung-Kook;Kim, Kyoung-Rae
    • Microbiology and Biotechnology Letters
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    • v.19 no.2
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    • pp.135-139
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    • 1991
  • Tridecanedioic acid (DC-13), starting material of the valuable musk ethylene brassylate, was obtained from n-tridecane by the Candida tropicalis mutant. The mutants were first obtained from primary screening step using the selective medium and then solid phase extraction sampling method was used for the selective isolation of organic acids from the cultured media of mutants. The resulting acids were directly converted to volatile tert-butyldimethyl silyl delivatives, which were then analyzed by gas chromatography. The efficient GC profiling method was used for the rapid identification of the mutant producing DC-13 in large quantity, and for the optimization of the culture conditions of mutant. The optimal culture conditions were found as follows: pH 8.0, 30$^{\circ}C$, 250rpm, 48hour of culture and $(NH_4)_2HPO_4$ as nitrogen source.

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A Study on the Treatment of Phenol Wastewater in an Anaerobic Fluidized-Bed Reactor (혐기성 유동층 반응기에서 페놀 폐수 처리에 관한 연구)

  • 박동일;안재동;신승훈;장인용
    • Journal of Environmental Health Sciences
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    • v.22 no.2
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    • pp.96-103
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    • 1996
  • The objectives of this study were to examine the biodegradation of phenol using the anaerobic fluidized bed reactor(AFBR). Mixed microorganisms were selected from the anaerobic digestion tank, and could be adapted to high concentration of phenol by increasing the phenol concentration 600-3600 mg/l step by step. The results were summarized as follows: 1. The average removal efficiency of phenol was 90%, decreased by increasing concentration of phenol, and then a shock range was 1200~2400 ppm. 2. The production rate of biogas in overall limits was proportional to the concentration of influent phenol. 3. At steady state, compositions of gases were $CH_4$ 55~60%, $C0_2$ 34~43%, respectively. These were similar to that of the theoretical estimates. 4. The production rates of biogas and methane per the molarity of phenol removed were linearly increased, 56.45 l gas/mol-phenol and 29.20 l $CH_4/mol$-phenol. Using this biogas, the recoverable energy was 269.1 kcal/mol phenol. It was 120.2 kcal/g-COD, transforming into the chemical oxygen demand. 5. The bulk of microorganisms existed in suspended section of fluidized bed with type of biofilm and its concentration was 340 mg/g-media. In conclusion, the anaerobic treatment of pure phenol was possible and its removal efficiency, introducing the AFBR, was successful. Also toxic organic compound such as phenol was biodegradable and was recoverable as resource of energy.

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Nutrients removal on Oxic/Anoxic time ratio in 2-stage-intermittent-aeration reactor (2단 간헐 포기조의 포기/비포기 시간비에 따른 영양염류 제거특성)

  • Kim, Hong Tae;Sin, Seok U;O, Sang Hwa;Gwon, Seong Hyeon
    • Journal of Environmental Science International
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    • v.13 no.7
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    • pp.675-680
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    • 2004
  • This study was conducted to remove organics and nutrients using 2 stage intermittent aeration reactor. First reactor, using suspended microbial growth in intermittent aeration instead of anaerobic reactor in the typical BNR process, used minimum carbon source to release P, and it was possible to reduce ammonia loading going to second reactor. In the second reactor, using moving media intermittent aeration, it was effective to reduce nitrate in non-aeration time by attached microorganisms having long retention time. In aeration time, nitrification and P uptake were taken place simultaneously. From the experiment, two major results were as follows. First, the removal of organics was more than 90%, and optimum aeration/non-aeration time ratio for organic removal was corresponded with aeration/non-aeration time ratio for nitrogen removal. Second, in the first reactor, optimum aeration/non-aeration time ratio was 15/75 (min.) because it was necessary to maintain 75 min. of non-aeration time to suppress of impediment of return nitrate and to lead release of phosphate. In the second reactor, optimum aeration/non-aeration time ratio was 45/90 (min.).

Photodegradation of VOCs by Using TiO$_2$-Coated POF (광촉매가 코팅된 플라스틱 광섬유을 이용한 VOC 광분해반응)

  • Ha, Jin-Wook;Joo, Hyun-Ku
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.4 no.3
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    • pp.199-203
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    • 2003
  • In this study plastic optical fibers (POFs) were considered as light-transmitting media and substrates for the potential use in photocatalytic environmental purification system. After the characteristics of POFs in terms of light transmittance and absorption were determined at the beginning, the further investigation was performed through the photocatalytic degradation of trichloroethylene (TCE), iso-propanol and etc. with TiO$_2$-coated optical fiber reactor systems (POFR). It is concluded that the use of POFs is preferred to quartz optical fibers (QOFs) since the advantages such as ease of handling, lower cost, relatively reasonable light attenuation at the wavelength of near 400nm can be obtained. Various geometrical reactor shapes have been constructed and applied for the last one and half years. For the use of POF in water phase treatment, however, more detailed scientific and engineering aspects should be envisaged. This case requires a suitable mixture to obtain more stable and innocuous immobilization of photocatalyst on POF. To overcome this disadvantage, metal-organic chemical vapor deposition (MOCVD) was conducted in a fluidized bed to deposit thin films of titania on glass and alumina beads. Those can be used as photocatalysis for the removal of pollutants especially in liquid phases.

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Analysis of Efficiency of Artificial Wetland for Waste Water Treatment Past Six Year Operation (6년 동안 운영한 인공습지의 처리효율 분석)

  • Hur, Jai-Kyou;Nam, Jong-Hyun;Kim, Yong-Jeon;Kim, In-Seon;Choi, Kyoung-Suk;Choi, Seung-Ik;Ahn, Tae-Seok
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.10 no.3
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    • pp.1-7
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    • 2007
  • For waste water treatment, artificial wetland was constructed in 1998. The size of artificial wetland is 20m${\times}$200m, with sand and gravel as media and Phragmites japonica was implanted. The removal rate of BOD, TN, and TP were 86%, 33% and 25% from June 2004 to November 2005 respectively, while those were 88%, 38% and 55% in 1999. Organic materials and nitrogen compounds are still effectively removed, after 6 years of construction, but the removal efficiency of phosphorus compounds is reduced. So for sustaining of artificial wetland as waste water treatment system, the removal efficiency of phosphorus compounds must be elevated.