• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 1978.10a
• /
• pp.207.2-207
• /
• 1978

Arthrobacter simplex (ATCC 6946) was cultured, induced and immobilized in acrylamide polymer. The characteristics of the immobilized whole-cell enayme were studied using hydrocortisone as the substrate. The enzyme activity was increased during the incubation of the gel particle in 0.5％ peptone media. The ennzyme reaction kinetics of the Δ'-dehydrogenase (3-oxosteroid Δ'-oxydo reductase, E. C. 1.3.99.4) foliowed the Michaelis-Menten type. Km and Vm values were different significantly after immobilization of the cell. The optimum pH and temperature were changed, too. Nitrogen sources such as casitone, peptone or tryptone were good media for the enzyme reaction. And there was no need to add cofactors of the enzyme in the pre-sence of energy sources used in the test. The effect of metal ions on the enzyme activity was insignificant. Organic solvents were used increase the substrate concentration and there was no optimum solvent concentration depending on the substrate concentration.

• Analytical Science and Technology
• /
• v.7 no.2
• /
• pp.149-153
• /
• 1994

An amperometric sensor for L-ascorbic acid(AA) in methanol media has been made by immobilizing squash-tissues on a graphite rod disk. A detection limit of the electrode was $2{\times}10^{-6}M$ L-ascorbic acid. In comparison with an isolated enzyme based ascorbate oxidase(AO) electrode, the plant-tissue electrode offered high biocatalytic stability and activity and extremely low cost. The electrode has a useful lifetime of 1 week.

• Microbiology and Biotechnology Letters
• /
• v.21 no.6
• /
• pp.615-621
• /
• 1993

In order to maximize the riboflavin production by a mutant strain Ashbya gosspyii, the optimization of medium and fed-batch fermentation were performed. As carbon sources, glucose and soybean oil were necessary for the riboflavin overproduction. Optimal concentrations of glucose and soybean oil in the flask cultures were found to be 3.0% and 0.5%, respectively, in a complex medium containing corn steep liquor(CLS) 1%. Among the various organic nitrogen sources tested, CSL was the most effective one both for the cell growth and riboflavin overproduction.

• The Journal of the Korea Contents Association
• /
• v.9 no.2
• /
• pp.173-180
• /
• 2009

In the recent past, the impression expressed by a human body expanded from that of aesthetic beauty to deformed organic medium influenced by environment. And the advancement in digital technology had made it possible to represent the deformed and distorted images of human body more realistically. Consequentially, these exaggerated physical images leave audiences with a feeling of grotesqueness. Chris Cunningham is a British director who mainly focuses on grotesque body as his subject matter using digital media. This paper will explore Chris Cunningham's work and his final goal through analyzing his carnivorous, uncanny grotesque body images, making use of the figital media, at each of his different levels.

• Water for future
• /
• v.27 no.4
• /
• pp.49-57
• /
• 1994

This study was carried out to obtain Kinetic constant of Moving Media Complete Mixing Activated Sludge (MMCMAS) for the wastewater treatment. A laboratory MMCMAS reactor was operated at the organic loading rate of 2.5g$BOD_{5}$/$m^{2}$ /d~48g$BOD_{5}$/$m^{2}$/d kinetic constant of MMCMAS reactor was estimated as follows:

• Journal of Environmental Health Sciences
• /
• v.13 no.2
• /
• pp.83-90
• /
• 1987

The objective of this study is to review the basic theories related substrate removal in wastewater flow variations using submerged biofilm reactor. An aerated biofilm reactor is that in which influent organic substrates are aerobically oxidized by the microorganisms of biofilm grown on the surface of submerged media. No sludge is returned, and oxygen is supplied by diffusers. Three types of aerated biofilm reactor are one stage-central aeration, one stageup flow aeration and two stage-side aeration. The orders of substrate removal capacity in wastewater flow variations showed two stage-side aeration, one stage-upflow aeration and one stage-central aeration. The phenonmenon of nonclosing volid in upflow aeration type was superior to these in central-side aeration type. Attached biofilm masses in case of upflow, side and central aeration reactor were 1.0mg/cm$^2$, 4.1 mg/cm$^2$ and 0.93 mg/cm$^2$, respectively. Yield coefficient for biofilm was 0.31 to 0.48. Especially, removal efficiency can be increased remarkably according to the number of biofilm reactor and the packed condition of media.

• Korean Journal of Plant Tissue Culture
• /
• v.27 no.5
• /
• pp.401-405
• /
• 2000

Influence of fructose addition to the cultivation medium on the production of taxanes and the growth of callus culture of Taxus baccata was studied. The cultures showed an ability to adjust to the substitution of some of the sucrose in the media by fructose and the fresh biomass accumulation was higher on the media containing different concentrations of fructose during the second cultivation period.

• Journal of environmental and Sanitary engineering
• /
• v.12 no.1
• /
• pp.69-75
• /
• 1997

Biological drinking water treatment is widely used in Europe for the removal of ammonia nitrogen and organics. During the last 16 years, the deterioration of the quality of surface waters used to produce drinking water has resulted in the widespread use of ozone-biological treatment in Korea. This study were conducted to determine the effect of media weight and preozonation on the biodegradability enhancement in biological fluidized bed(BFB) using Han river water. When the carbon weight was increased, $NH_{3}-N$ and DOC removal increased, but turbidity and SS removal decreased. To remove turbidity and SS, the bed depth in 40% expansion rate/total bed depth was very important. Preozonation of raw water was not effective in $NH_{3}-N$, but increased in biodegradable organic fraction about 10-30% with 0.425-0.85 mg $O_{3}/mg$ DOC.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.35 no.4
• /
• pp.67-75
• /
• 1993

This study was carried out to investigate the applicability of Solid Contact Process for the improvement of Moving Media Complete Mixing Activated Sludge(MMCMAS) effluents. Laboratory MMCMAS Reactor and MMCMAS/Solid Contact were operated at the hydraulic loading of 122~340 L/m$^2$/d. The conclusions from this study were as follows ; The addition of Solid Contact tank to the MMCMAS reactor has increased the SCOD and SBOD removal efficiencies of 4 to 67% and 2 to 41%, respectively. In addition, the increments of nitrification rates were about 13 to 46%. It was also observed that the addition of Solid Contact tank has greatly increased the organic removal efficiencies at the higher hydraulic loading rates and also decrement of sludge production rates was 0.1 gVSS/gBODrem. It was therefore concluded that the addition of Solid Contact tank could have polished the effluent of MMCMAS at the higher hydraulic loading rates.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2009.10a
• /
• pp.889-892
• /
• 2009

We have developed a practical printing technology for the gate electrode of organic thin film transistors(OTFTs) by combining screen-printing with wet-etching process using nano-silver ink as a conducting material. The screen-printed and wet-etched Ag electrode exhibited a minimum line width of ~5 um, the thickness of ~65 nm, and a resistivity of ${\sim}10^{-6}{\Omega}{\cdot}cm$, producing good geometrical and electrical characteristics for gate electrode. The OTFTs with the screen-printed and wet-etched Ag electrode produced the saturation mobility of $0.13cm^2$/Vs and current on/off ratio of $1.79{\times}10^6$, being comparable to those of OTFT with the thermally evaporated Al gate electrode.