• Korean Journal of Organic Agriculture
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• v.20 no.2
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• pp.185-200
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• 2012

It has been reported that world population continues to increase so that a matter of food security can be a world-wide problem for mankind. An anticipated rise in world population of 30% and the subsequent increased demand for food brings with it challenges in terms of global resource usage and food security. However, ruminant livestock production and consumption make a large contribution to the greenhouse gas (GHG) emissions which can be attributable to food production. Given the association between GHG and climate change, this is clearly of great concern to the livestock industry worldwide. Nevertheless, ruminant livestock also play an important role in global food security as they can convert the plant cell wall materials and non-protein nitrogen compounds, found widely in plants but indigestible to all monogastric animals including man, into high value proteins for human consumption. Much effort has been made to maximize animal production, feed conversion ratio, and to improve animal breeding in ruminant agriculture. In addition improving feed formulation techniques, developing chemical additives, plant extracts, and new plant varieties for grazing have been tested. Future ruminant production systems will need to capitalize on important benefits of ruminants. It is therefore suggested that ruminant agriculture has a key role to play in maintaining and enhancing provision of quality proteins and essential nutrients for human being but the challenge of reducing GHG emissions, and methane in particular, needs to be successfully addressed.

• Korean Journal of Organic Agriculture
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• v.23 no.1
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• pp.77-89
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• 2015

133 Species of medicinal plants were used for the development of natural agrichemicals with anti-microbial activity against Acidovorax avenae subsp. citrulli, a pathogen of bacterial fruit blotch in watermelon. The MeOH-extracts of these medicinal plants were examined for anti-microbial activity by bioassay. The MeOH-extract of Citrus unshiu Markovich had the strongest antibacterial activity against Acidovorax avenae subsp. citrulli. To identify anti-microbial compounds from Citrus unshiu Markovich, solvent-fractionation was used. The fraction of hexane, which showing the highest value of anti-microbial activity, was analyzed by GC-MS. Each mass spectra, corresponding to each peak of chromatogram, was compared to mass database of Wiley library. As a result, d-Limonene, ${\gamma}$-terpinene, ${\beta}$-linalool, terpineol, palmitic acid, 9,12-octadecadienoic acid, Linolenic acid, and stigmasterol were identified. Among them, d-Limonene, ${\gamma}$-terpinene, ${\beta}$-linalool, and terpineol confirmed to be shown the anti-microbial activity by bioassay. Especially, d-Limonene and ${\gamma}$-terpinene found to have strong activity. In conclusion, we thought d-limonene and ${\gamma}$-terpinene from Citrus unshiu Markovich. Latin, had anti-microbial activity against Acidovorax avenae subsp. citrulli and could be candidates for the control agents for the control of bacterial fruit blotch in watermelon.

• Korean Journal of Organic Agriculture
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• v.20 no.1
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• pp.49-58
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• 2012

Greenhouse whitefly, $Trialeurodes$ $Vaporariorum$, is the most important insect pest in environmental friendly tomato production. Natural enemies, $Nesidiocoris$ $tenuis$ and environmental friendly agricultural materials (EFAM) are major control agents in Korea. The aim of the present work is to evaluate release time of $N.$ $tenuis$ in controlling of $T.$ $vaporariorum$ and to select environmental friendly agricultural materials (EFAM) to suppress both $T.$ $vaporariorum$ and $N.$ $tenuis$ which if the density were high causing necrotic rings. Six EFAM selected to control of $Trialeurodes$ $Vaporariorum$ in three times spray with seven days intervals. Three EFAM showed high mortality 67.7-70.5% in spray after release plot but low in release after spray plot. It should be careful to spray with $N.$ $tenuis$. When $N.$ $tenuis$ were early release at 7 days after transplanting which before occurring and establishment of greenhouse whitefly, it could suppress the density. Extracts of bead tree + matrin, matrin 1, Chrysanthemum showed high mortality on $T.$ $vaporariorum$ adults and $N.$ $tenuis$. The results suggested that this three EFAM could be control agents of the damage of necrotic rings from $N.$ $tenuis$.

• Applied Biological Chemistry
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• v.14 no.1
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• pp.99-102
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• 1971

These experiment were conducted to study the effects of various nitrogen sources and inorganic salts upon the growth of Saccharomyces rouxii T-9, and the results obtained were as follows: 1) The strain showed the most rapid growth in the case of 2.5 to 5.0% addition of peptone as an organic nitrogen source, and 1.0% addition of $(NH_4)_2HPO_4$ as an inorganic nitrogen source respectively to the media containing no NaCl. 2) Its growth was the most rapid in the case of 1.5% addition of yeast extracts as an organic nitrogen sources, and 5% addition of urea as an inorganic nitrogen source respectively to the media containing 20% of NaCl. 3) Its growth was the most rapid in the case of 1% addition of $NaNO_3$ as an inorganic salt to the media containing no NaCl, while to the media containing 20% or 26% of NaCl its growth was more rapid in the case of 5% addition of $NaNO_3$ than 1% addition. 4) Its growth was more rapid in the case of 0.5% addition of $MgSO_4\;and\;CaCl_2$ respectively to the media containing 20% of NaCl than to the media containing no NaCl, and was rapid in the case of 1% addition of $MgCl_2$ to the media containing 26% of NaCl.

• Food Science and Preservation
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• v.20 no.3
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• pp.342-347
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• 2013

In recent years, polyphenol-rich herbs, fruits and processed foods, which are made of plant origin, have attracted much attention due to their potential health benefits. Pomegranate (Punica granatum L.) is an important source of bioactive compounds and has been used to treat diseases because of its medicinal properties. This research was focused on characterizing Korea's national cultivar and a similar product from California, USA. To evaluate their bioactive compounds and pharmacological activities, their anti-oxidation and cancer inhibition properties, as well as their organic acid and free sugar contents, were investigated. The national cultivar had low total sugar and high organic acid contents, contrary to the imported product. The results showed that the peel of national cultivar had high polyphenol and ellagic acid contents compared to imported product. The free radical scavenging capacity was evaluated via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and its positive correlation with the total polyphenol contents was found. The anti-cancer activity of methanol extracts revealed growth inhibition against the prostate cancer cell. These results signify that while pomegranate, national cultivar, is more sour than the imported product, its health benefits could be excellent. Also, the polyphenol compound content of the non-edible part (such as the peel and the seed) was higher than that of the juice. Thus, it is suggested that the byproduct of the juice extraction could be potentially used in other fields such as medicine or dietary agents.

• KSBB Journal
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• v.19 no.6 s.89
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• pp.427-432
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• 2004

The purpose of this study is to investigate the feasibility of a cosolvent-modified supercritical $CO_2\;(scCO_2)$ extraction technique for the production of licorice extracts with high levels of glabridin. The effects of various parameters such as the type and amount of modifiers, extraction temperature ($40{\sim}80^{\circ}C$) and pressure ($10{\sim}50.0\;MPa$) on the extraction efficiency were examined at a fixed flow rate of 1 mL/min. The organic solvent extraction with pure methanol was also conducted for a quantitative comparison with the $scCO_2$ extraction. The recovery of glabridin from licorice was found to be extremely small for pure $scCO_2$. However, the addition of modifiers such as ethanol and acetone to $scCO_2$ resulted in a significant improvement in the recovery of glabridin. The recovery of glabridin was observed to increase with pressure at a constant temperature. Furthermore, the purity of the glabridin obtained from the $scCO_2$ extraction was higher compared with the organic solvent extraction.

• Korean Journal of Organic Agriculture
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• v.23 no.3
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• pp.535-547
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• 2015

In this study, the chemical properties and phenolic compound of blueberry, bokbunja and mulberry and their pomace were determined to develop them as functional food materials. Water content of individual whole berry was ranged from 84.25-86.20%, and water content was significantly high in whole berries rather than their pomace (p<0.01). Additionally, each berry and its pomace's pH was 3.32-5.18. Among them, whole mulberry showed the highest pH which is 5.18 (p<0.01). Total polyphenol and flavonoid contents were the greatest in blueberry pomace and they were 24.81 mg/g and 2.13 mg/g, respectively (p<0.01). However, mulberry pomace generated the greatest anthocyanin content compared to others (p<0.01). In phenolic compound profiles, cyanin chloride was detected in mulberry and bokbunja. Epigallocatechin, gallocatechin and isorhamnetin were found only in blueberry. Catechin (hydrate) and epicatechin were greater in pomaces than whole berries except blueberry (p<0.01), otherwise, significantly great rutin (trihydrate) and quercetin contents were found in whole berries as compared to their pomace except blueberry (p<0.01). Gallic acid was significantly greatest in mulberry (p<0.01) and quercetin 3-D-galactoside was significantly greatest in blueberry (p<0.01). Apigenin and luteolin were traced in mulberry, and mulberry pomace showed greater apigenin and luteolin contents than whole mulberry (p<0.01). Naringenin was greater in pomaces than whole berries (p<0.01). As a result, it was found that all berry extracts used in this study were able to be applied as functional food materials and their pomace contained high phenolic compound enough to be a good source of phytochemical for nutraceutical use.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.4
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• pp.514-520
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• 2019

This study investigated the improved lipid metabolism effect of 3T3-L1 cells induced by adipocytes using the dichloromethane (DCM) fraction in the organic solvent extract of Wassong (Orostachys japonicus). To confirm the cell cytotoxicity, each of 6 fractions of organic solvent extracts (EtOH, Hexane, DCM, EtOAc, BuOH, and H₂O) was examined using MTS assay. As a result, it was confirmed that the DCM extract was stable over the whole range of concentrations, and a DCM fraction was used to confirm the improved lipid metabolism effect. Lipid excretion was measured to confirm the change of lipid metabolism. 3T3-L1 cells induced by adipocytes were treated with DCM extract and stained with oil-red O to evaluate lipid accumulation. As a result, it was confirmed that the lipid efflux was significantly improved. In order to confirm the mechanism of lipid efflux, the mRNA expressions of ABCA1 and ABCG1, which are lipid transport proteins, were confirmed by real-time PCR. Therefore, the present study confirmed that the DCM extract from Orostachys japonicus has the effect of improving the lipid metabolism on 3T3-L1 adipocytes. In addition, the results of this study will be used as the basis for the development of functional foods using Orostachys japonicus and also for conducting research on the detailed mechanisms.

• Journal of the Korean Applied Science and Technology
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• v.36 no.1
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• pp.341-347
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• 2019

In this study, the following is the result of measuring the quality characteristics of herbal wine and the active inhibition of Glutathione S-transferase in order to measure the release of physiological active substances according to the concentration of extracts. The pH level of herbal wine was 4.4, up from 3.9 before fermentation. These changes are attributed to fermentation and organic acids during alcoholic fermentation. The acidity of herbal wine was 0.55%, about six times higher than the pre-fermentation control of 0.09%. These results show that organic acids are used for flavor formation, ether, in combination with alcohol. The inhibitory activity of glutathione S-transferase were $5.1{\pm}0.31$ in herbal wine 15%, $6.5{\pm}0.6$ in herbal wine 20%, $7.6{\pm}0.6$ in herbal wine 25%, $8.4{\pm}0.2$ in herbal wine 30% and $9.7{\pm}0.7$ in herbal wine 35%. As the extract concentration was increased the inhibitory activity of glutathione S-transferase were significantly increased (<0.05).

• Korean Journal of Organic Agriculture
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• v.29 no.4
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• pp.613-623
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• 2021

This study was conducted to investigate the effect of addition levels of coffee and green tea by products extract including polyphenols through hot water extraction on rumen fermentation. The treatment groups consisted of coffee extract (CO), green tea extract (GR) and mixed extract (MIX), and the addition level was 10 µL, 20 µL and 30 µL of three levels. The experiment consisted of a total of 10 experimental groups including the control group, and a full factorial design was used. The effect of polyphenol addition in coffee and green tea by-products was analyzed through main and interaction effect of statistical analysis. The total polyphenol content of the extracts was 106.15, 79.10 and 185.25 ㎍ GAE/g DM for coffee by-product, green tea by-product and mixture, respectively. Total gas production was significantly lower in the treatment groups than in the control (114.00 mL/gDM) (p<0.05). Methane emission tended to decrease as the polyphenol addition level increased. Moreover, the MIX showed the lowest methane emission when 30 µL was added (p<0.05). Volatile fatty acids showed a significant difference compared to the treatment group as a control (98.06 mM) (p<0.05), but there was no change according to the level of polyphenols. As a result of the main effect and interaction, it is thought that the effect on methane reduction and improvement of rumen fermentation in MIX20 can be expected. In a series of studies, the addition of 20 µL of a blended extract of coffee and green tea by-products is thought to reduce methane to levels that do not inhibit rumen fermentation.