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Anti-inflammatory Effects and its Mechanisms of Hesperidin in an Asthmatic Mouse Model Induced by Ovalbumin

  • Chang, Jeong-Hyun
    • 대한의생명과학회지
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    • 제16권2호
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    • pp.83-90
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    • 2010
  • Hesperidin, a member of the flavanone group of flavonoids, can be isolated in large amounts from the rinds of some citrus species [e.g., Citrus aurantium L. (bitter orange), Citrus sinensis L. (sweet orange) and Citrus unshiu Marcov. (satsuma mandarin)], and has been reported to have anticarcinogenic, antihypotensive and antimicrobial properties. Despite the efficacy of these polyphenolic compounds as immune modulators, the effects of the flavonoids are poorly understood about allergic effect. In this study, we investigated whether hesperidin could influence on Th1 and Th2 balance. Allergic reactions included an increase in the number of eosinophils in bronchoalveolar lavage (BAL) fluid, an increase in inflammatory cell infiltration into the lung tissue around blood vessels and airways, airway luminal narrowing, the development of airway hyper-responsiveness (AHR). The administration of hesperidin before the last airway OVA challenge resulted in a significant inhibition of all asthmatic reactions. Accordingly, this study may provide evidence that hesperidin plays a critical role in the amelioration of the pathogenetic process of asthma in mice. These findings provide new insight into the immunopharmacological role of hesperidin in terms of its effects in a murine model of asthma, and also broaden current perspectives in our understanding of the immunopharmacological functions of hesperidin.

전자 디스플레이에서의 변별력에 근거한 정보 코딩용 색상의 도출 (A Study on Salient Colours for Information Coding at Electronic Video Displays)

  • 김상호;양승준;박관석
    • 대한안전경영과학회:학술대회논문집
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    • 대한안전경영과학회 2003년도 춘계학술대회
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    • pp.135-141
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    • 2003
  • An experiment was conducted to identify salient colours for information coding at electronic video displays. CRT and TFT-LCD were used to evaluate the effect of different types of electronic displays on the salience of colours. Total of 100 Subjects, 50 for each display were asked to select more salient 10 colours among 24 given colours. There was no statistically significant difference in the salience of colours between the two display types. The result showed that the tested colours could be clustered into 5 categories according to their brightness, saturation, and the number of R, G, B elements occupied to reproduce the colours. Three achromatic colours (black, white, grey) and eight chromatic colors (red, yellow, green, blue, cyan, orange, magenta, and indigo) were identified as the salient colours at the electronic video displays. The result also showed that the eight chromatic colours could be clustered into two distinct categories, landmark colours(red, yellow, green, and blue) and the other basic colours (cyan, magenta, orange, and indigo). It is noticeable that cyan, magenta, and indigo substituted for pink, purple, and brown that were recommended as the salient colours for the environment not using electronic video displays by the previous researches.

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감귤의 Bioflavonoids 분리, 정제 및 혈압강하효과 (Isolation, Purification and Hypotensive Effect of Bioflavonoids in Citrus sinensis)

  • 손흥수;김현숙;권태봉;주진순
    • 한국식품영양과학회지
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    • 제21권2호
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    • pp.136-142
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    • 1992
  • 냉동 건조한 귤(Citrus sinensis)의 껍질로 부터 methanol과 butanol추출물인 crude bioflavonoids를 건조중량으로 최초 시료대비 약 0.26%의 수율을 얻을수 있었다. 이 crude bioflavonoids를 gel filtration, HPLC를 이용하여 분리, 정제하였고 TLC, HPLC, UV spectrum, NMR spectral analysis를 통해 narirutin과 hesperidin으로 동정할 수 있었다. 또한 HPLC 정량분석 결과, narirutin과 hesperidin은 crude bioflavonoids의 g당 42mg과 530mg을 얻을 수 있었으며 귤 껍질의 주요 bioflavonoid는 hesperidin으로 판명되었다. 한편 실험 동물인 Sprague-Dawley종의 흰쥐를 대상으로 혈압 변화를 측정한 결과 귤 껍질의 주요 bioflavonoid성분인 hesperidin은 약물 투여 후 유의성있게 (p< 0.001) 혈압 저하효과가 있음을 관찰 할 수 있었다.

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Removal of Methylene blue from saline solutions by adsorption and electrodialysis

  • Lafi, Ridha;Mabrouk, Walid;Hafiane, Amor
    • Membrane and Water Treatment
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    • 제10권2호
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    • pp.139-148
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    • 2019
  • In this study, the removal of MB from saline solutions was evaluated by two methods by adsorption and electrodialysis; the adsorption of the mixture dye/salt on dried orange peel waste (OPW) was studied in batch method. In this study the biosorption of cationic dye by OPW was investigated as a function of initial solution pH, and initial salt (sodium chloride) concentration. The maximal dye uptake at $pH{\geq}3.6$ in the absence and in the presence of salt and the dye uptake diminished considerably in the presence of increasing concentrations of salt up to 8 g/L. The Redlich Peterson and Langmuir were the most suitable adsorption models for describing the biosorption equilibrium data of the dye both individually and in salt containing medium. As well, this work deals with the electrodialysis application to remove the dye. Synthetic solutions were used for the investigation of the main operational factors affecting the treatment performance; such as applied voltage, pH, initial dye concentration and ionic strength. The experimental results for adsorption and electrodialysis confirmed the importance of electrostatic interactions on the dye. The electrodialysis process with standard ion exchange membranes enabled efficient desalination of cationic dye solutions; there are two main factors in fouling: electrostatic interaction between cations of dyes and the fixed charged groups of the CEM, and affinity interactions.

Armillaria 속균을 이용한 천마의 생산 (Production of Gastrodia elata Tuber using Armillaria spp.)

  • 성재모;정범식;양근주;이현경
    • 한국균학회지
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    • 제23권1호통권72호
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    • pp.61-70
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    • 1995
  • Armillaria 속균은 천마를 생산하는데 아주 중요한 균이다. 한국에서는 천마재배지의 골목과 이병수목 그리고 그 곳에서 발생한 자실체로부터 72균주가 분리 되었다. 이들 분리균에 대한 정확한 biological species를 분류하기 위하여 tester를 이용하여 교배시험을 한 결과 A. mellea가 2균주로 이들 균주는 천마재배포장에서 분리 된 균주이며 이제까지 채집한 자실체에서 분리된 균주에서는 A. mellea가 없었다. A. tabescens는 7균주로 치악산에서는 신나무, 속초에서는 해송의 그루터기와 강원대 연습림의 참나무에서 형성된 자실체에서 분리되었으며 A. ostoyae는 24균주로 주로 본 병원균에 침입을 받아 죽은 10년생 전후의 잣나무의 지표면 부위에 형성된 자실체에서 분리되었다. A. gallica는 39균주로 주로 참나무 등 14 종류의 기주식물 그루터기에 형성된 자실체에서 분리되었다. Armillaria 속균들은 배양적 특성에 따라서 4가지 Group 들로 분리되었으며 Group II인 KNU-A110(A. gallica)은 다른 Group들에 비해 균사의 생장과 균사속 형성이 대단히 왕성하였고 천마의 생산에도 우수한 것으로 판별되었다. 이 균들은 식물조직에 있는 mycelial fan과 천마 사이를 균사속에 의하여 연결하고 있다. 또한 재배 방법에 있어서는 종균접착법이 가장 좋았다. 천마는 강원도 내의 13개 지역에서 발견되었다. 천마는 줄기의 색에 의하여 3가지 종류로 구별되었으며 줄기색이 brownish orange와 greyish yellow는 G. elata로 판별되었고 줄기색이 greyish green 인 것은 G. gracilis로 판별되었다. 천마는 주로 5월 중순에서 6월 중순 사이에 낙엽이 덮여진 부식토층의 남향 경사지에서 채집되었다. 자마는 성숙한 천마가 싹이나고 썩으며 생산되든지 다른 자마로부터 출아로 생산되었다. 자마는 균사속으로부터 영양분을 얻어서 자라게 되며 자마가 성숙한 천마가 되기까지는 약 3년이 소요된다.

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Comparative Investigation of Flavors in Cigarettes by Electronic Nose and GC/MS

  • Lee, Yelin;Park, Jin-Won;Lee, Hwan-Woo;Lee, Seung-Yong;Lee, Hyung-Suk
    • 한국연초학회지
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    • 제35권1호
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    • pp.20-27
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    • 2013
  • An Electronic Nose(E-Nose) and Gas Chromatography/Mass Spectroscopy (GC/MS) are meanwhile conventional technique to analyze volatile materials in many industries (e.g., food, medicine, environment) and have broad acceptance in the analysis of tobacco products. In this study, an experiment where tin oxide gas sensor array responses and GC/MS profiles are used to characterize the volatile compounds of different cigarettes at the same time is performed and the measurements of two instruments are compared for cigarette samples with a known chemical information. E-Nose and GC/MS were employed to differentiate and match flavored cigarettes with commercial tobacco flavoring agents (lavender, vanilla, peppermint, orange, star anise). For verifying reliability of two systems, the analyses were conducted in terms of amount of flavors in each cigarettes using partial least squares (PLS) and with the principal components analysis (PCA). Various chemical sensors and GC/MS data was reduced into two principal factors (PC1, PC2) for being distinguished with visualized regions. Both systems provided adequate results for odor characteristics of cigarettes in this study with each instrument having its own advantages and disadvantages.

Synthesis of Nanorod g-C3N3/Ag3PO4 Composites and Photocatalytic Activity for Removing Organic Dyes under Visible Light Condition

  • Se Hwan Park;Jeong Won Ko;Weon Bae Ko
    • Elastomers and Composites
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    • 제59권1호
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    • pp.1-7
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    • 2024
  • Nanorod graphitic carbon nitride (g-C3N4) was synthesized by reacting melamine (C3H6N6) with trithiocyanuric acid (C3H3N3S3) in distilled water for 10 h at room temperature. The resulting mixture was calcined at 550℃ for 2 h in an electric furnace under an air atmosphere. Nanorod g-C3N4/Ag3PO4 composites were prepared by adding nanorod graphitic carbon nitride (g-C3N4) powder, silver nitrate (AgNO3), ammonia (NH3·H2O, 25.0-30.0%), and sodium hydrogen phosphate (Na3HPO4) to distilled water. The samples were characterized via X-ray diffraction, scanning electron microscopy, and Fourier-transform infrared spectroscopy. The photocatalytic activities of the nanorod g-C3N4/Ag3PO4 composites were demonstrated via the degradation of organic dyes, such as methylene blue and methyl orange, under blue light-emitting diode irradiation and evaluated using UV-vis spectrophotometry.

식미 우수한 조생종 살구 '초하(初夏)' ('Choha', an Early-maturing and High-quality Apricot)

  • 강상조;정경호;김호열;전지혜;이돈균
    • 원예과학기술지
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    • 제17권1호
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    • pp.21-22
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    • 1999
  • '초하(初夏)'는 농촌진흥청 원예연구소에서 1986년에 'N.Y. 472'에 'Early Orange'를 교배하여 얻은 교잡종자로부터 유래된 조생, 내열과성 살구 품종으로, 1992년에 1차선발하고 1995년부터 3년간 '원교 사-05' 계통명으로 지역적응성을 검토하여 1997년에 최종선발 및 명명된 살구 품종이다. 이 품종은 성숙전 강우에 의한 열과 및 당도 저하가 없으며, 과피가 적황색으로 착색되어 외관이 우수하고, 식미가 좋다. 숙기는 6월 하순으로 우리 나라의 살구 재배품종 중에서는 가장 빠르며, 당도는 $12.5^oBx$로 높고, 산미가 적으며, 과중은 60g 내외로 조생종 살구 품종으로서는 큰 편이다.

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Evaluation of a Visible Implant Fluorescent Elastomer Tag in the Greenling Hexagrammos otakii

  • Park, In-Seok;Kim, Young Ju;Gil, Hyun Woo;Kim, Dong-Soo
    • Fisheries and Aquatic Sciences
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    • 제16권1호
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    • pp.35-39
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    • 2013
  • The aim of this study was to assess visible implant fluorescent elastomer (VIE) tagging in greenling Hexagrammos otakii. The experiental fish were anesthetized individually and marked with orange, yellow, red, and green elastomer at the following five body locations, respectively: the adipose eyelid, the surface of the dorsal fin base, the inside surface of the pectoral fin base, the inside surface of the pelvic fin base, and the surface of the anal fin base. Control fish were anesthetized but not marked. During the 20-month trial, fish growth and retention, underwater visibility, and readability of the tags were determined. After 20 months, body length of marked greenling ($43.2{\pm}3.5cm$, mean ${\pm}$ standard deviation [SD]) did not differ from that of the control ($41.4{\pm}3.7cm$). Additionally, the body weight of marked greenling ($527.4{\pm}39.8g$, mean ${\pm}$ SD) did not differ from that of the controls ($505.9{\pm}31.7g$). Greenling retained >90% of the tags at the surface of the dorsal fin base. The anal fin base showed a higher tag retention rate than the inside surfaces of the pectoral fin and the pelvic fin bases (P < 0.05). Red and orange tags were identified more easily underwater than green and yellow tags. Green and yellow tags emitted fluorescence in response to a narrower range of light wavelengths. Thus, the VIE mark was easy to apply to greenling (< 1 min per fish) and was readily visible when viewed under an ultraviolet lamp.

키틴 기반 흡착제 PEI-chitin을 이용한 반응성염료의 제거 (Removal of Reactive Dyes using Chitin-based Adsorbent PEI-chitin)

  • 김경민;왕쭈오;원성욱
    • Korean Chemical Engineering Research
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    • 제57권2호
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    • pp.232-238
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    • 2019
  • 산업폐수 속의 대표적인 색도유발물질인 염료를 효과적으로 제거하기 위한 생체흡착제로 폴리에틸렌이민을 키틴에 가교결합한 PEI-chitin을 개발하였다. 대표적인 반응성염료인 Reactive Orange 16 (RO16)을 모델염료로 사용하였고, RO16에 대한 PEI-chitin의 흡/탈착 능력을 평가하기 위해 pH의 영향, 등온흡착, 흡착속도론, 탈착 실험을 수행하였다. 그 결과, Langmuir 식에 의해 산출된 최대흡착량은 pH 2에서 266.3 mg/g이었고, 흡착평형에 도달하는 시간은 50 mg/L에서는 약 20분, 100 mg/L에서는 약 60분 그리고 200 mg/L에서는 약 240분으로 평가되었다. 탈착실험은 암모니아/에탄올 혼합용액, NaOH, $NaHCO_3$, $Na_2CO_3$를 용리액으로 이용하여 평가하였으며, 암모니아/에탄올 혼합용액에서 75.24%로 가장 높은 탈착율을 보였다.