• 한국수산과학회지
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• 제52권4호
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• pp.358-365
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• 2019

This study investigated the effects of dietary cactus Opuntia ficus-indica stem and fruit extract on the growth, flesh quality, lysozyme activity, and histological changes of growing Korean rockfish Sebastes schlegeli. Three replicates of fish (152 g/fish) were fed one of the following diets: containing 0 additions (control); 0.1, 0.5, or 1.0% cactus stem powder; or 1.0% fruit extract for 11 weeks. Growth performance did not differ significantly among treatments, including survival, final weight, feed efficiency, and daily feed intake. The experimental diets did not affect the proximate and fatty acid compositions, plasma biochemistry, or dorsal muscle texture of the fish. However, the plasma lysozyme activity of the fish fed the diet containing 0.1% cactus stem was significantly higher than that of the fish fed the control diet. These fish had variously sized lipid vacuoles in the liver tissue compared with the control. Distinct mucosal folds and mucus-secreting goblet cells developed in the fish fed the diet containing 1% cactus stem compared with the other dietary groups. These results suggest that feeding growing Korean rockfish cactus stem might increase the plasma lysozyme activity and induce histological changes in the gastrointestinal tract that might be related to digestion.

• 한국식품저장유통학회지
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• 제6권3호
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• pp.345-359
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• 1999

백년초(손바닥 선인장)는 제주도를 비롯한 우리나라의 남해안에서 많이 생산되는 선인장의 일종으로 오래전부터 그 임상효능이 알려져 여러 가지 기능적 특성을 갖는 것으로 생각된다. 따라서 본 연구에서는 추출용매 및 방법에 따른 백년초 추출물에 대한 항균 및 항산화성에 대하여 조사하였다. 백년초의 항균활성은 대체적으로 환류추출법이 상온에서의 진탕추출법보다 조금 강하게 나타났고, 각 용매간에는 큰 차이가 없었다. 또한 모든 시험균주에 대하여 항균활성이 강하게 나타났고, 특히 Pseudomonas aeruginosa균주에 대해서는 진탕추출법에 의한 추출물이, Escherichia coli O-157균주에는 환류추출법에 의한 추출물이 상대적으로 다른 시험균주에 비하여 강한 항균활성을 나타내었다. 백년초 물추출물을 Salmonella enteritidis에 처리하였을 때 균체의 표층구조가 허물어지는 심한 형태학적 변화를 나타내었다. 백년초 추출물의 첨가시 0.1% BHT의 첨가시 보다는 약하였지만 상당한 정도의 수소공여능을 나타내었으며, linoleic acid에 대한 항산화력의 조사에서도 0.1% BHT 첨가시와 같은 정도의 강한 효과를 나타내었다.

• 한국식품영양과학회지
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• 제41권7호
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• pp.1009-1013
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• 2012

신선절단 사과에 E. coli O157:H7과 L. monocytogenes를 인위적으로 접종한 후 백년초 추출물 처리에 따른 저장 중 미생물 수 변화를 조사하였다. 신선절단 사과에 접종된 E. coli O157:H7과 L. monocytogenes의 초기 균수가 5~6 log CFU/g이 되게 하였고, 사용한 백년초 추출물은 25, 50, 100, 200 mg/mL이었으며 4, $21^{\circ}C$에서 8일간 저장하였다. E. coli O157:H7은 백년초 추출물 50 mg/mL 이상 처리구에서 유의적으로 E. coli O157:H7 생육저해 효과가 나타났음을 확인할 수 있었으며, 온도별로는 $21^{\circ}C$ 저장 시에 생육 억제효과가 더 큰 것으로 판단되었다. L. monocytogenes는 E. coli O157:H7에 비교하여 매우 민감하게 반응하였으며 저장 온도별 차이는 크지 않았다. 특히 50 mg/mL 이상 처리구에서 2일째부터 4, $21^{\circ}C$ 저장 시 모두 L. monocytogenes가 검출한계 이하로 관찰되어 생육억제에 매우 효과적임을 확인할 수 있었다. 그리고 관능평가에서는 100~200 mg/mL 이상의 백년초 추출물 처리가 신선절단 사과의 품질을 잘 유지하는 것으로 나타났다. 따라서 본 연구결과, 백년초 추출물 처리는 신선 절단 사과의 저장, 유통 중 오염될 수 있는 E. coli O157:H7과 L. monocytogenes의 생육억제에 매우 효과적인 천연물이며, 향후 미생물학적 안전성 확보와 신선식품의 품질유지 향상에 효과적인 천연물 항균제로서 개발 가능하리라 판단된다.

• 한국식품위생안전성학회지
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• 제17권2호
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• pp.71-78
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• 2002

항생제 및 항균 물질 등의 오, 남용으로 인하여 항생제 내성균들이 등장, 사회적으로 큰 문제가 되고 있으며, 최근에는 식물이나 probiotics 등과 같이 항생제를 대체할 수 있는 물질에 대한 연구가 활발히 진행되고 있다. 이에 본 연구에서는 국내에서 오래 전부터 민간 요법으로 사용되어 오던 백련초 (Opuntia ficus-indica var. saboten)를 이용하여 in vitro와 in vivo에서 Salmonella enteyica serova. Enteritidis (S. enteritidis), S. enterica serova. Typhimurium (S. Typhimurium) DT104 및 Escherichia coli O157:H7에 대한 항균능을 평가하였다 백련초의 수용액이 포함된 Moulter Hinton agar에 Salmonella spp. 및 S. coli O157:H7를 접종한 결과 이들 균에 대한 억제능(inhibitory activity)을 확인할 수 있었다. 백련초 제제를 경구투여한 군에서 분변으로 배출되는 S.typhimurium DT104의 수가 급격히 감소하였고, 낀 후에는 더 이상 분변으로 균이 배출되지 않았으며, intestinal IgA가 유의적으로 증가함이 확인되었다. 본 연구를 통하여 백련초가 in vitro에서 S. enteritis, S. Typhimurium DT104와 E. coli O157:H7대한 항균력을 나타낼 뿐만 아니라 마우스의 gastrointestinal tract에서 역시 Salmonella에 대한 항균력을 보임을 확인할 수 있었다. 본 연구를 통해 백련초 및 그 성분을 이용한 병원성 세균의 제거 및 면역 증진 효과 등과 같은 연구의 필요성이 확인되었고, 그 응용 가능성도 매우 높을 것으로 사료된다.

• Biomolecules & Therapeutics
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• 제15권4호
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• pp.205-211
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• 2007

The fruits and stems of Opuntia ficus-indica var. saboten have been reported to exhibit a variety of pharmacological actions, including antioxidant, analgesic, anti-inflammatory, and anti-ulcer effects. In the present study, we evaluated effects of the butanol fraction (SK OFB901) prepared from the 50% ethanol extract of the stems on various types of neuronal injuries induced by oxidative stress, excitotoxins, and amyloid ${\beta}\;(A_{\beta})$ in primary cultured rat cortical cells. Its antioxidant and radical scavenging activities were also evaluated by cell-free bioassays. We found that SK OFB901 strongly inhibited the oxidative neuronal damage induced by $H_2O_2$ or xanthine/xanthine oxidase. In addition, it exhibited marked inhibition of the excitotoxic neuronal damage induced by glutamate, N-methyl-D-aspartic acid, or kainate. Furthermore, the $A_{\beta(25-35)}$-induced neurotoxicity was also significantly attenuated by SK OFB901. It was found to inhibit lipid peroxidation initiated by $Fe^{2+}$ and L-ascorbic acid in rat brain homogenates and scavenge 1,1-diphenyl-2-picrylhydrazyl free radicals. These results indicate that the butanol fraction prepared from the stems of Opuntia ficus-indica var. saboten exerts potent antioxidant and neuroprotective effects through multiple mechanisms, implying its potential applications for the prevention or management of neurodegenerative disorders associated with oxidative stress, excitotoxicity, and $A{\beta}$.

• 한국임상수의학회지
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• 제22권1호
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• pp.43-49
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• 2005

The aim of this study is to investigate the wound healing effects of the Opuntia ficus-indica. Four full-thickness skin wounds(2.5 cm × 2.5 cm) were made in different areas on the back in each of five dogs. Two wounds were used for wound size measurement and two for skin biopsy. The ten full-thickness wounds were treated with the extract of Opuntia ficus-indica, and the ten full-thickness wounds were treated with normal saline. The wound areas were measured every other day for three weeks after wounding. Statistical analysis was performed with the paired t-test. The wound healing rates were 32.9±4.0%, 28.2±7.1%, 42.2±4.1%, 55.0±4.3%, 76.6±2.8%, 86.8±2.3%, 94.0±1.l%, 97.3±0.8%, 99.3±0.3%, 99.8±0.2%, 100.0±0.0% in the experimental group, and 18.0± 8.2%, 13.S± 11.6%, 15.2±6.1 %, 36.9±8.8%, 62.4±4.1%, 80.0±2.3%, 86.0±1.4%, 94.0±0.9%, 96.9±0.8%, 97.8±0.3%, 99.7±0.1% in the control group on the 1/sup st/, 3/sup rd/, 5/sup th/, 7/sup th/, 9/sup th/, 11/sup th/, 13/sup th/, 15/sup th/, 17/sup th/, 19/sup th/, 21/sup st/ day. On the 17/sup th/ day, the wound closed above 99.9% in 1 dog of the experimental group. On the 19/sup th/ day, the wound closed above 99.9% in 3 dogs of the experimental group. On the 21/sup st/ day, the wound closed above 99.9% in 5 dogs of the experimental group, and 1 dog of the control group. The wound healing period was shortened by 2 to 4 days in the experimental group as compared with the control group. The tensile strength was 4,508±513 g/㎠ in the experimental group and 2,772±400 g/㎠ in the control group on the 21/sup st/ day after wounding. The tensile strength was significantly higher in the experimental group than in the control group(p< 0.05). Inflammation rapidly spread and disappeared in the experimental group but not in the control group. Fibroblast and vascularization were found on the 5/sup th/ day in both group and increased only in the experimental group on the 9/sup th/ day. The firs finding of epithelialization was on the 13/sup th/ day in the experimental group and the 21/sup st/ day in the control group. A significantly higher wound healing rate(p< 0.05), tensile strength(p< 0.05) and better histopathological findings were observed in the experimental group than in the control group throughout the experimental period. These results suggest that topical application of Opuntia ficus-indica extract can promote wound healing in dogs.

• 한국생활과학회지
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• 제21권6호
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• pp.1211-1222
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• 2012

This research studied the effects of Backryeoncho (Opuntia ficus-indica var. saboten) extract as an additive in kimchi in various amounts (0%, 0.4%, 0.8%, 1.2%), and its acceptance by middle school students in the Jeonju area. The treated kimchi had a higher pH than the untreated kimchi without extract, and the titratable acidity of the kimchi decreased with the addition of the Backryeoncho extract. The degree of salinity decreased in kimchi with the added Backryeoncho extract in a dose-dependent manner. The cutting force and compression force were higher in kimchi where the extract was added during the fermentation period resulting in improved texture of the kimchi. The addition of the extract slowed growth of total bacteria (1.83~2.12 log CFU/mL) and lactic acid bacteria (1.4 log CFU/mL) as compared with the control sample. In the sensory evaluation of overall taste, kimchi with 0.8% extract added demonstrated higher evaluation in overall categories. The acceptability of Backryeoncho extract as an additive in kimchi among middle school students was higher than in the control sample, with an optimum additive of 0.4% extract, based on the lowest volume of leftovers.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1316-1321
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• 2009

Multiple beneficial properties of Opuntia ficus indica (OPF) are well established. In the present study, we have investigated the immunological role of OPF extract (OPFE) on murine splenocytes. OPFE dose- and time-dependently enhanced the proliferation of splenocytes without cytotoxicity. Our results also showed that the number of $CD4^+$ helper T cells and CD45R/$B220^+$ pan B cells increased markedly, but not $CD8^+$ cytotoxic T cells or $CD11b^+$ granulocytes/macrophages. In addition, OPFE significantly decreased the production levels of T helper (Th) 1 type cytokines, interferon (IFN)-$\gamma$, and tumor necrosis factor (TNF)-$\alpha$, although had no significantly differences in those of interleukin (IL)-4, a Th2 type cytokine in concanavalin A (Con A)-stimulated blastogenic cells. Furthermore, OPFE alone strongly increased IL-4 production and decreased TNF-$\alpha$ production even in the absence of Con A. On the basis of these results, this study suggests that OPFE enhances immunity by regulating the pro- and anti-inflammatory response, indicating that this extract exerts a marked immunomodulatory effect, confirming its usefulness as therapy for immune-related diseases.

• Food Science and Biotechnology
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• 제14권2호
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• pp.190-195
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• 2005

The conditions for the extraction of effective substances from the stem of Opuntia ficus-indica were optimized by a response surface methodology. The total extract yield was maximized under the temperature of $97.77^{\circ}C$, at a time duration of 145.82 min and a water to sample ratio 16.59 mL/g. Moreover, the optimum conditions for the extraction of effective substances were as follows: $84.95^{\circ}C$, 156.50 min and a water/sample ratio of 7.46 mL/g for the phenolics content; and $97.11^{\circ}C$, 139.03 min and a water/sample ratio of 10.91 mL/g for the pectin content. The range of optimum extraction conditions in consideration of the physicochemical properties of the extracts were shown to be as $95-100^{\circ}C$ as the extraction temperature, 120-180 min as extraction time and a water to sample ratio of 5-18 mL/g.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.299.3-300
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• 2002

Both of Semen (OF-Se) or stem (OF-Sf) of Opuntia ficus-indica Semen have been used as a healthful food or folk medicine in korea for the treatment of asthma. diabetes mellitus. aging, osteoporosis, rheumatic arthritis. constipation, cancer. gastric ulcer. constipation. toxic state, edema, etc, There are many reports that OF have the anti-gastric damage, wound healing, diabetes mellitus, monoamine oxidase B inhibitor etc. (omitted)