• 한국산림과학회지
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• 제101권3호
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• pp.443-453
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• 2012

본 연구는 아까시나무 유묘에서 뿌리혹 형성에 가장 적절한 배양토의 종류와 토양의 양료 수준을 구명하기 위해 수행하였다. 상업용 상토, 완숙 혹은 미숙 퇴비, 유기질 비료, 산림용 고형비료, 화학비료 등의 토양 첨가물을 이용하여 질소와 인의 수준을 다양하게 조절한 후 포트에 아까시나무 종묘를 식재하여 3개월 간 온실에서 실험을 실시하여 초기 뿌리혹 형성에 적절한 토양 환경을 조사하였다. 피트모스, 질석, 지올라이트를 함유한 상업용 상토는 높은 보수력으로 인해 초기 활착과 건중량 생산에는 적절하였으나 무기 양료 중 질소(0.052%), 인(91 ppm)의 부족으로 인해 뿌리혹 형성에는 부적합하였다. 퇴비는 미숙 퇴비이건 완숙퇴비이건 구별 없이 뿌리혹 형성에 가장 유리했는데, 질소(0.08-0.21%)와 인(141-1,228 ppm)의 함량이 적절하였지만, 칼륨(0.14-1.58 cmol/kg)의 함량은 부족한 편이었다. 화학(복합)비료는 토양 내 질소(0.14-0.21%), 인(406-618 ppm), 칼륨(1.39-3.13 cmol/kg)의 함량을 적절한 수준으로 유지하여 초기 건중량 생산에는 지장을 주지 않았지만 뿌리혹 형성에는 부적절하였다. 산림용 고형비료는 뿌리혹 형성에 적합한 반면, 유기질 비료는 뿌리혹 형성에 적합하지 않았다. 배양토에는 적절한 수준의 질소(0.05-0.2%)와 인(100-600 ppm)이 함유되어 있을 경우에 초기 뿌리혹 형성이 제대로 이뤄졌다. 아까시나무 유묘의 경우 토양의 질소와 인 중에서 질소보다 인이 초기의 뿌리혹 형성을 더 촉진하는 것으로 판단되었다.

• Applied Biological Chemistry
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• 제38권2호
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• pp.123-128
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• 1995

Agrobacterium rhizogenes를 매개로 한 Populus의 형질전환계를 확립하고자 형질전환율에 영향을 주는 주요 인자들을 검토하고, 형질전환 식물체를 분화시켜 도입유전자의 발현을 확인하였다. 줄기조직보다 잎조직이 kanamycin에 대한 감수성이 커 형질전환체의 선발에 유리한 것으로 판단되었다. 접종용액의 박테리아 밀도는 $4{\times}10^5{\sim}7{\times}10^9\;cfu$의 범위내에서 형질전환율에 거의 영향을 주지 않았다. 공배양 기간은 1일 이내가 바람직하였고, 박테리아 제거배지의 항생제 농도는 cefotaxime과 ampicillin 각각 $250\;{\mu}g/ml$가 적당하였다. 배지에 acetosyringone을 첨가하여 배양한 박테리아를 사용했을 때 형질전환율이 증가했는데 acetosyringone의 적정농도는 $50\;{\mu}M$이었다. 형질전환된 gall은 kanamycin 농도가 $100\;{\mu}g/ml$ 이상인 배지를 사용하거나, 생장조절제가 들어있지 않은 배지를 사용하여 선택적으로 유기 및 증식시킬 수 있었다. A. rhizogenes를 접종시킨 조직으로부터 유기된 gall은 생장조절제를 포함하는 배지뿐만 아니라, 생장조절제가 없는 배지에서도 3주 이내에 뿌리를 형성하였다. NAA 0.05 mg/ml와 BA 0.5 mg/ml를 첨가한 배지에서 배양된 gall로 부터 약 6주일 후 식물체가 분화되었다. A. rhizogenes를 접종시켜 얻은 gall,그리고 gall로부터 재분화된 식물체의 추출물에서 agropine과 mannopine이 검출되어, 도입된 opine합성 유전자가 발현되고 있는 것이 확인되었다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.235-238
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• 2000

Paecilomyces japonica의 균체 생육 및 다당체 생성에 미치는 기본배지 및 물리적인 조건에 대한 영향을 조사하였다. 기본배지로는 균체량과 다당체 생성이 가장 좋은 YMP medium로 결정하였고 배양온도는 $27^{\circ}C$, pH는 9, 접종량은 2%로 선택하였다.

• 한국미생물·생명공학회지
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• 제25권1호
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• pp.75-81
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• 1997

Streptomyces chibaensis J-59 did not grow in the culture medium containing only xylose or xylan as a carbon source, because it was defective in xylulokinase production; xylB mutant. S. chibaensis J-59 was able to produce xylanase and $\beta $-xylosidase as well as glucose isomerase. The glucose isomerase in S. chilbaensis J-59 was induced in the medium containing xylan or xylose which could be utilized as an inducer but not sa carbon and energy sources. So we tried to produce glucose isomerase whthout consumption of xylose or xylan as an inducer by using xylB mutant S. chilbaensis J-59. The optimum condition for the production of the glucose isomerase was attained in a culture medium composed of 1% xylan, 0.15% glucose, 1.5% corn steep liquor, 0.1% MaSO$_{4}$ $\CDOT $7H$_{2}$O, and 0.012% CoCL$_{2}$ $\CDOT $ 6H$_{2}$O(pH 7.0). The production of the enzyme reached to a maximum level when the bacteria were cultured for 42 h at 30$\circ $C. The enzyme production in a jar fermentor was increased twice as much as that in a flask culture.

• Journal of Plant Biotechnology
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• 제6권2호
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• pp.125-130
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• 2004

Callus and cell suspension cultures of Eurycoma longifolia Jack could be an alternative supply of 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one. The callus tissues were initiated from leaves of different trees. The friable calli were used for the preparation of the cell suspension cultures of E. longifolia. The leaf explant of tree Eu-9 produced the most callus and also induced high cell biomass in the cell suspension culture, but it produced low quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. The leaf explant from tree Eu-8 produced low quantity of callus and cell biomass, but produced the highest quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. Optimum production of cell biomass was obtained on cell culture medium with pH 5.75 prior to autoclaving, but high alkaloids content could be induced in culture medium in acidic condition with pH 4.75 and 5.25 prior to autoclaving.

• 한국광학회지
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• 제5권1호
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• pp.67-73
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• 1994

재생증폭기에서의 gain narrowing 현상, 비선형형상, 에너지 포화상태 등의 증폭특성 해석 및 최적 설계를 위한 시뮬레이션 코드를 개발하였다. 재생증폭기에서 gain narrowing 현상을 줄이기 위해서는 증폭매질의 중심파장에 일치하는 대칭인 펄스를 이득대역폭이 넓은 매질에 입사시켜야 한다. 재생증폭기의 특성을 최대한 활용할 수 있는 증폭매질로는 이득대역폭이 넓고, 비선형굴절율이 작으며, 고에너지를 얻을 수 있는 Ti-Sapphire가 최적매질로 평가되었고, 재생증폭기 설계시 중요한 파라미터에 대하여 해석하였다.

• 한국미생물·생명공학회지
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• 제22권6호
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• pp.651-658
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• 1994

For the production of digoxin by using biotransformation in suspension-cultured Digita- lis lanata cells, a two-stage culture process was optimized. Modified Murashige and Skoog medium was used for growth in the first stage and the cells were transferred to glucose solution for the production of digoxin from digitoxin via biotransformation in the second stage. When the cells were cultivated for 10 days in the growth period, 12$\beta$-hydroxylation capacity was the best. It was found that the optimum amount of digitoxin as substrate was 400 mg/l with initial cell density of 21%. Maximum productivity was achieved 5 days after transfer of cells to production medium. Sucrose and fructose provided similar digoxin yield as that in glucose, and 6% was proved to be the best glucose solution. Most of the components of modified MS medium except phosphate reduced the efficiency of digoxin formation. Besides, peptone and beef extracts inhibited 12$\beta$-hydroxylation, while promoting glucosylation. Finally, it was apparent that light enhanced the formation of digoxin significantly.

• Food Science and Biotechnology
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• 제16권1호
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• pp.159-162
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• 2007

Optimization of the fermentation medium for phenylethyl alcohol (PEA) production by Pichia anomala SKM-T was performed. The carbon source (glucose), nitrogen source (L-phenylalanine), and initial pH value were independent variables of the optimized medium. The central composite rotatable design was used for the experimental design and the analysis of the results. The optimum medium composition for the maximal production (621.27 mg/L) of PEA was found to be an initial pH of 5.03, and concentrations of L-phenylalanine at 6.53 and glucose at 6.11 g/L (w/v). This experimental finding is in close agreement with the model prediction (702.79 mg/L; desirability 0.884) with an 11.6% difference.

• KSBB Journal
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• 제13권4호
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• pp.399-403
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• 1998

This study was carried out to optimize the concentration of Ca2+ and pH of fusion medium which affected electrofusion frequency of protoplasts isolated from Nicotiana tabacum L. (cv. BY4) mesophyll cells and callus. The protoplasts were electrofused in the fusion media containing two different Ca2+ concentrations and three different pH regions. Fusion frequency was lower in the fusion medium containing only 13% mannitol as osmotic stabilizer. However, higher degree of fusion frequency (47.3%) was observed in the fusion medium containing 50mM CaCl2 at pH 10.5 than any other conditions. Cell viability was decreased by Ca2+ and high pH treatment in the fusion media, while fusion frequency was increased. It is concluded that Ca2+ is involved in electrofusion of protoplasts.

• 원예과학기술지
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• 제29권5호
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• pp.488-493
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• 2011

Callus culture initiation of strawberry (Fragaria${\times}$ananassa Duch.) was investigated at different Murashige and Skoog (MS) medium strengths, types and concentrations of plant growth regulators, and incorporating a cold pretreatment period to determine the optimal nutritional and environmental conditions. No high quality callus was induced on MS media without auxin regardless of medium strength. When 6-benzylaminopurine (BA) was combined with indole acetic acid (IAA), naphthalene acetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D), high quality callus were highly induced compared to medium supplemented with auxin alone. When $0.5mg{\cdot}L^{-1}$ BA was combined with IAA, NAA, and 2,4-D, high quality callus induction was more effective than the medium supplemented with the other BA concentrations. The best combination of auxin and cytokinin for high quality callus induction was $1.0mg{\cdot}L^{-1}$ NAA and $0.5mg{\cdot}L^{-1}$ BA. Although the differences in callus induction were not significant, high quality callus induction at half strength MS medium was more effective than at full strength medium. When $30g{\cdot}L^{-1}$ sucrose was added to the half strength MS medium, the rate of high quality callus induction increased. The optimum cold pretreatment temperature and period for high quality callus induction were $4^{\circ}C$ and 72 h, respectively. Regeneration rate of high quality callus increased in MS medium supplemented with thidiazuron.