• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.632-637
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• 2000

This research was undertaken to restore the biological activity of cyclodextrin glucanotransferase (CGTase) of Bacillus macerans origin expressed as inclusion bodies in recombinant Escherichia coli. The optimum concentration of urea used as a denaturant was 8 M. The supplementation of 0.5 M urea into a dialysis buffer increased the refolding efficiency by preventing any protein aggregation. The influence of the protein concentration, temperature, and pH were also investigated. The protein concentration was found to be the most important factor in the refolding efficiency. The optimum temperature was 15-$25^{\circ}C$ and the optimum pH was 6.0. The maximum specific activity of the CGTase refolded under the optimum conditions was 92.2 U/mg, corresponding to 72% of the native CGTase. A comparison of the secondary structure between the native and the refolded CGTase showed that the relative ratio of the $\alpha$-helix content in the native to the refolded CGTase was 1:0.82.

• Journal of Industrial Technology
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• v.27 no.B
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• pp.161-167
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• 2007

The objetives of this study are to set up optimum extraction temperature, time and organic solvent for propolis extraction, to investigate chemical properties, and to develop health foods from propolis preparation. In this study, ethanol and ultrasonic extracts method performed to optimum extraction temperature was at 60, $20^{\circ}C$, optimum extraction time was at 12, 4 hours and optimum extraction amount of solvent was at 20, 15 times of propolis weight. When various ethanol solutions were used, whereas flavonoid content was highest in 70, 80% aqueous ethanol, respectively. So the ultrasonic extracts method used gave better results than the ethanol extracts method in this work. Extraction of propolis with etanol and ultrasonic extracts method was performed by using the water and various concentrations of aqueous ethanol as solvent. Sensitivity of propolis samples to Staphylococcus aureus was investigated and the results were shown. Samples of water extract did not inhibit microbial growth, where as 50% aqueous ethanol extract the largest inhibitory zone for Staphylococcus aureus, then decreased inhibition with increasing ethanol concentrations.

• Nuclear Engineering and Technology
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• v.54 no.9
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• pp.3235-3241
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• 2022

The Korea Atomic Energy Research Institute (KAERI) is planning the construction of the KIJANG Research Reactor (KJRR) for stable supply of ⁹⁹Mo. The Fission ⁹⁹Mo Production Process (FMPP) of KJRR produces solid waste such as spent uranium cake and alumina cake, and liquid waste in the form of intermediate level liquid waste (ILLW) and low level liquid waste (LLLW). This study thus established the operating range and optimum operating conditions for the cementation of ILLW from FMPP. It also evaluated whether cement waste form samples produced under optimum operational conditions satisfy the waste acceptance criteria (WAC) of a disposal facility in Korea (Korea radioactive waste agency, KORAD). Considering economic feasibility and safety, optimum operational conditions were achieved at a w/c ratio of 0.55, and the corresponding salt content was 5.71 wt%. The cement waste form samples prepared under optimum operational conditions were found to satisfy KORAD's WAC when tested for structural stability and leachability. The results indicate that the proposed cementation conditions for the disposal of ILLW from FMMP can be effectively applied to KJRR's disposal facility.

• Magazine of the Korean Society of Agricultural Engineers
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• v.16 no.4
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• pp.3600-3610
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• 1974

Experimental work of batch-type dryer was conducted to develop its optimurm operating conditions by analyzing the major factors which affect the drying performance. A laboratory batch-type dryer was constructed and tested for various levels of heated-air rates, and depths of grain deposit. Tong-il rice variety having the initial moisture content of approximaely 23 per cent in wet basis was used for the experiment. The criteria selected for establishing the optimum operating condition were the drying performance rate, the thermal efficiency, and the operational cost of the dryer. The results of the study are summarized as follows: 1. The performance rate of dryer for a specific operating condition was defined as total amount of material dried per hour when the moisture content of grains in the upperlayer reaches to 16 per cent in wet basis. The optimum operating conditions as viewed in the rate of drying performance could be justified by functional realtionship between the depth of grain deposit and air flow rate. In other words, there was a definite depth of grain deposit for a given air-rate which make the dryer performance maximum. The optimum grain depth for the batch-type dryer with 3.3㎡ loading area and with the attached axial fan was about 35cm. 2. The thermal efficiency for the dryer was evaluated by the ratio of the latent heat required to evaporate the grain moisture to the heat input required to raise the ambient air-temperature to 40 degree centigrade. The optimum operating condition as viewed in term of thermal efficiency analyzed was that grater depth and lower air flow-rate may be desirable. This condition is contracted with the optimum condition as viewed by the dryer performance rate. 3. The annual operating cost of batch-type dryer was analyzed for different annual hour of use and for different operation condition. The optimum condition as viewed in terms of operating cost was almost identical to one as viewed in terms of dryer performance rate. Therefore, the most economical use of batch-type dryer for the same annual operating hours can be obtained when the dryer operated in the condition of maximum dryer performance rate. Increasing the annual operating hour may be desirable to cut down the dryer operation cost, since the annual hour of dryer use is much sensitive to the operating cost than any peractical conditions of dryer operation. 4. The most desirable operational condition as justified by combining all the criteria, dryer performance rate, thermal efficiency and annual operating cost, could be concluded to operate the dryer in the condition of maximum performance rate. The condition in general is identical to the lowest operation cost for a given annual operating hour.

• KCID journal
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• v.5 no.1
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• pp.63-74
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• 1998

This study was performed to evaluate the hydraulic conductivity of sand-bentonite mixtures according to 8 kinds of the bentonite content using the reassembled air-entry permeameter. Results obtained are as follows: 1. The maximum dry density and optimum m

• Magazine of the Korean Society of Agricultural Engineers
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• v.20 no.1
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• pp.4599-4613
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• 1978

This study was intended to investigate the effects of bituminous material content of soil-cement mixtures on their durability. For the purpose, unconfined compressive strength test, Freeze-thaw test, and wet-dry test were performed with three types of soil. Each type of soil was mixed with three levels of cement content and each soil-cement mixture was mixed with four levels of bituminous material content. For the unconfined compressive strength test, Freeze-thaw test and wet-dry test, 324, 108, and 108-specimens were prepared respectively. Unconfined compressive strength was measured at age of 7-days, 14-days and 28-days using 108-specimens in each age. The soil-cement loss rate due to freeze-thaw and wet-dry were calculated after 12 cycles of test using 108-specimens in each test. The results are summarized as follows : 1. Optimum moisture content was increased with increase of cement content, but maximum dry density was changed irregulary with increase of the cement content. 2. The unconfined compressive strength was increased with increase of cement content, bituminous material content and curing age. Cement is more effective factor than bituminous material on unconfined compressive strength of soil-cement Mixture. 3. It is estimated as the most economical cement content that the recommended cement content of A.S.T.M. because increasing rate of unconfined compressive strength at age of 28-days was low when cement content is above the recommanded cement content of A.S.T.M. among all types of soil. 4. Although a portion of cement content is substituted for bituminous material, the necessary unconfined compressive strength can be obtained. 5. The soil-cement loss was more influenced by wet-dry than Freeze-thaw 6. The bituminous material is more effective on the decrease of soil-cement loss than increase of unconfined compressive strength 7. The void ratio of soil-cement mixture was changet irregularly with increase of cement content, but that was decreased in proportion to the increase of bituminous material content. 8. The regression equation between the unconfined compressive strength and soil-cement loss rate were obtained as table 7.

• Current Research on Agriculture and Life Sciences
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• v.31 no.2
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• pp.88-93
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• 2013

The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 $kg/ha^-$ ($N-P_2O_5-K_2O$, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15.

• Journal of Crop Science and Biotechnology
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• v.10 no.3
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• pp.133-140
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• 2007

Protein characteristics of Korean wheat were evaluated to determine the effects of protein content and quality on processing and textural properties of white salted noodles compared to US wheat flours with various wheat classes and commercial flours for making noodles. Protein quality parameters, which were independent of protein content and included SDS sedimentation volume with constant protein weight, mixograph mixing time and proportion of 50% 1-propanol insoluble protein, of Korean wheat flours with 2.2+12 subunits in high molecular weight glutenin subunit compositions were comparable to those of commercial flours for making noodles. Parameters related to noodle making, including optimum water absorption, thickness and color of noodle dough sheet, correlated with protein content and related parameters, including SDS sedimentation volume with constant flour weight, mixograph water absorption and gluten yield. No significant relationship was found in protein parameters independent of protein content. Hardness of cooked noodles from Korean wheats was lower than that of US wheat flours compared to similar protein content of commercial noodle flours. Adhesiveness, springiness and cohesiveness of cooked noodles from Korean wheats were similar to US wheat flours. Hardness of cooked noodles correlated with protein content and related parameters.

• Elastomers and Composites
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• v.50 no.2
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• pp.87-91
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• 2015

Cure characteristics of non-oil-extended solution SBR (S-SBR_NO) and oil-extended solution SBR (S-SBR_ EO) compounds reinforced with silica/carbon black were investigated. Minimum and maximum torques of S-SBR_ EO compounds were much smaller than those of S-SBR_NO ones. Delta torques of S-SBR_NO compounds were greater than those of S-SBR_EO ones, and that of S-SBR_NO compound increased with increase in the 1,2-unit content. Optimum cure time ($t_{90}$) of S-SBR compound decreased with increase in the 1,4-unit content, whereas it increased with increase in the 1,2-unit content. Cure rate index of S-SBR compound increased with increase in the 1,4-unit content, whereas it decreased with increase in the 1,2-unit content. Comparing cure characteristics of S-SBR_NO and S-SBR_EO compounds with the same 1,2-unit content, $t_{90}$s of S-SBR_EO compounds were longer than those of S-SBR_NO ones and cure rate indices of S-SBR_EO compounds were smaller than those of S-SBR_NO ones.

• Textile Coloration and Finishing
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• v.31 no.3
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• pp.177-186
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• 2019

In this study, we investigated for synthetic hydrotalcite in chloroprene rubber foam. Experiments were carried out to find the optimum content ratio by controlling the contents of MgO and Hydrotalcite. Swelling test in toluene immersion was made to measure the crosslinking density of CR foams, and the cure properties were investigated with flat die rheometer and Mooney viscosity. The difference of hardness, tensile strength and elongation at break were observed after immersing in 7% NaCl or 21% NaCl solutions for a day and four days. In addition, the volume change and water content remaining in CR foam were measured after immersing NaCl solution. As content of MgO increased, the value of the cure torque tended to increase, but it was almost constant above 2phr of MgO. However, the Mooney viscosity decreased with increasing MgO content. The crosslinking density, determined by the swelling ratio, showed that the CR compound without MgO showed a higher degree of swelling. When the content of hydrotalcite/MgO was 3:2, it was the lowest volume change of CR form. Also, As the content of hydrotalcite decreased, the difference of mechanical properties before and after immersion NaCl solution increased.