• Convergence Security Journal
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• v.17 no.5
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• pp.3-10
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• 2017

Standard Protocol Optimized for Resource-Constrained IoT Environment Constrained Application Protocol (CoAP) supports web-based communication between a sensor node in the IoT environment and a client on the Internet. The CoAP is a Request / Response model that responds to the client's CoAP Request message by responding with a CoAP Response message from the server. CoAP recommends the use of CoAP-DTLS for message protection. However, validation of the use of DTLS in the IoT environment is underway. We analyze CoAP and DTLS security mode, evaluate performance of secure channel creation time, security channel creation step time, and RAM / ROM consumption through Cooja simulator and evaluate the possibility of real environment application.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.19 no.2
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• pp.1-7
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• 2019

Recently, multimedia streaming service has been activated, and the demand for high-quality multimedia convergence contents services is predicted to increase significantly in the future. The issues of the increasing network load due to the rise of multimedia streaming traffic must be addressed in order to provide QoS guaranteed services. To do this, an efficient network resource management and mobility support technologies are needed through seamless mobility support for heterogeneous networks. Therefore, in this paper, an MIH technology was used to recognize the network situation information in advance and reduce packet loss due to handover delays, and an ACLMIH-FHPMIPv6 is designed that can provide an intelligent interface through introducing a hierarchical mobility management technique in FPMIPv6 integrated network.

• Molecules and Cells
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• v.44 no.8
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• pp.602-612
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• 2021

DNA methylation is an important epigenetic mechanism affecting genome structure, gene regulation, and the silencing of transposable elements. Cell- and tissue-specific methylation patterns are critical for differentiation and development in eukaryotes. Dynamic spatiotemporal methylation data in these cells or tissues is, therefore, of great interest. However, the construction of bisulfite sequencing libraries can be challenging if the starting material is limited or the genome size is small, such as in Arabidopsis. Here, we describe detailed methods for the purification of Arabidopsis embryos at all stages, and the construction of comprehensive bisulfite libraries from small quantities of input. We constructed bisulfite libraries by releasing embryos from intact seeds, using a different approach for each developmental stage, and manually picking single-embryo with microcapillaries. From these libraries, reliable Arabidopsis methylome data were collected allowing, on average, 11-fold coverage of the genome using as few as five globular, heart, and torpedo embryos as raw input material without the need for DNA purification step. On the other hand, purified DNA from as few as eight bending torpedo embryos or a single mature embryo is sufficient for library construction when RNase A is treated before DNA extraction. This method can be broadly applied to cells from different tissues or cells from other model organisms. Methylome construction can be achieved using a minimal amount of input material using our method; thereby, it has the potential to increase our understanding of dynamic spatiotemporal methylation patterns in model organisms.

• Journal of Ginseng Research
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• v.45 no.1
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• pp.58-65
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• 2021

Background: Panax ginseng, as one of the most widely used herbal medicines worldwide, has been studied comprehensively in terms of the chemical components and pharmacology. The proteins from ginseng are also of great importance for both nutrition value and the mechanism of secondary metabolites. However, the proteomic studies are less reported in the absence of the genome information. With the completion of ginseng genome sequencing, the proteome profiling has become available for the functional study of ginseng protein components. Methods: We optimized the protein extraction process systematically by using SDS-PAGE and one-dimensional liquid chromatography mass spectrometry. The extracted proteins were then analyzed by two-dimensional chromatography separation and cutting-edge mass spectrometry technique. Results: A total of 2,732 and 3,608 proteins were identified from ginseng root and cauline leaf, respectively, which was the largest data set reported so far. Only around 50% protein overlapped between the cauline leaf and root tissue parts because of the function assignment for plant growing. Further gene ontology and KEGG pathway revealed the distinguish difference between ginseng root and leaf, which accounts for the photosynthesis and metabolic process. With in-deep analysis of functional proteins related to ginsenoside synthesis, we interestingly found the cytochrome P450 and UDP-glycosyltransferase expression extensively in cauline leaf but not in the root, indicating that the post glucoside synthesis of ginsenosides might be carried out when growing and then transported to the root at withering. Conclusion: The systematically proteome analysis of Panax ginseng will provide us comprehensive understanding of ginsenoside synthesis and guidance for artificial cultivation.

• Korean Journal of Agricultural Science
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• v.47 no.4
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• pp.717-726
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• 2020

The physical characteristics of the major varieties of perilla were analyzed to use as basic data for the design of a high-quality, high-efficiency perilla cleaner and stone separator. Because the size, thousand-grain weight, angle of repose, angle of friction, bulk density and terminal velocity of perilla have significant differences according to the perilla variety, the different of characteristics by variety should be considered for performance improvement of a perilla cleaner and stone separator. Therefore the cleaner and stone separator using a sieve could be improved by the application of a detachable sieve or by using equipment such as a 2 - 3 stage sieve and regulating the slope. Moreover, because differences in the terminal velocity occur due to the differences in the size and thousand-grain weight according to the perilla variety, a blower with an adjustable fan speed was considered for the design of the improved cleaner. Additionally, it was shown that the length of perilla has the greatest correlation based on a comparison of the coefficients of the other characteristics. Accordingly, the length of perilla could be used as a major factor for the fine adjustment and parts replacement of the device. These results can be used as basic data for a high-quality, high-efficiency perilla cleaner and stone separator. In the future, the development of the machine and follow-up studies based on the basic data are needed to determine the optimized operating conditions and mechanism of action.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.14 no.1
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• pp.14-21
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• 2021

Green networking has become a issue to reduce energy wastes and CO2 emission by adding energy managing mechanism to wired data networks. Energy consumption of the overall wired data networks is driven by access networks, expect for end devices. However, on a global scale, it is more difficult to manage centrally energy, measure and model the real energy use and energy savings potential of the access networks. This paper presented the multiple linear regression model to predict energy consumption of wired access networks using supervised learning of machine learning with data collected by existing investigated materials, actual measured values and results of many models. In addition, this work optimized the performance of it by various experiments and predict energy consumption of wired access networks. The performance evaluation of the regression model was achieved by well-knowned evaluation metrics.

• Journal of the Korean Institute of Gas
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• v.26 no.2
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• pp.9-13
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• 2022

Electric wheelchairs, the output from the motor is mainly applied to a speed reducer using a power transmission device such as a belt or a chain. However, although a speed reducer using a belt or chain is a simple device, it occupies a lot of space and has a space limitation, so it is not suitable for an electric wheelchair driving part. However, since the speed reducer of the planetary gear type is decelerated on the same axis, the volume can be reduced, so the space constraint is less than that of the belt or chain type reducer. Therefore, in this study, a driving part that can obtain great propulsion with a speed reducer using a planetary gear type was developed through a study on the driving part of a wheelchair that can be switched between manual and electric. Accordingly, the tooth shape of the planetary gear applied to the reducer was designed using the Kisssoft program. In addition, the drive part was designed to be applicable to the existing wheelchair wheels, and the mechanism was optimized for the manual/electric switching principle and operation principle of the drive part. Based on the research contents, the final design and manufacture of the wheelchair reducer drive unit in the form of a planetary gear having one sun gear, two planetary gears and one ring gear was carried out.

• Journal of the Korea Society for Simulation
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• v.19 no.2
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• pp.17-28
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• 2010

Since a homing torpedo system consists of various subsystems, organic interactions of which dictate the performance of the torpedo system, it is necessary to estimate the effects of individual subsystems in order to obtain an optimized design of the overall system. This paper attempts to gain some insight into the detection mechanism of a torpedo run, and analyze the relative importance of various parameters of a torpedo system. A database for the analysis was generated using a simulation model based on the combined discrete event and discrete time architecture. Multiple search schemes, including the snake-search method, were applied to the torpedo model, and some parameters of the torpedo were found to be stochastic. We then analyzed the effectiveness of torpedo’s detection capability according to the torpedo speed, the target speed, and the maximum detection range.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.3A
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• pp.323-330
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• 2008

The ship collision analysis of steel pile group as protection system of bridge in navigable waterways was performed to analyze the structural characteristics of protective structure during ship collision. The analysis encompassed finite element modeling of ship and pile, modeling of material non-linearity, hard impact analysis, displacement-based analysis and soft impact analysis for collision scenarios. Through the analysis of hard impact with a rigid wall, impact load for each collision type of ship bow was estimated. In the displacement-based analysis the estimate of energy which protection system can absorb within its maximum horizontal clearance so as to secure bridge pier from vessel contact during collision was performed. Soft impact analysis for various collision scenarios was conducted and the collision behaviors of vessel and pile-supported protection system were reviewed for the design of protection system. The understanding of the energy dissipation mechanism of pile supported structure and colliding vessel would give us the optimized design of protective structure.

• Journal of the Korean Society of Safety
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• v.39 no.1
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• pp.1-8
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• 2024

Commercial gas stoves feed primary air to the burner and burn the fuel-air mixture in a partially premixed combustion. This mechanism produces carbon monoxide during combustion. In this study, design parameters of a commercial gas stove were optimized by considering the carbon monoxide emission. Gas consumption rate, carbon monoxide emission, and water boiling temperature as a heating performance were determined. Carbon monoxide emission was measured using a Korean Industrial Standards standard collector. Water boiling temperature was measured by first soaking the pot in water for approximately 10 min and then heating the pot filled with water. A thermocouple was installed inside the pot. Carbon monoxide increased as the nozzle diameter was increased and the burner-pot height was decreased. This result was due to the insufficient mixing between the fuel and air. Heating performance was enhanced when the nozzle diameter was increased and the burner-pot height was decreased. However, the heating performance deteriorated when the nozzle diameter was 1.8 mm and the burner-pot height was reduced to 50 mm. This phenomenon was due to the formation of a flame on the side of the pot. A merit factor was defined to find the optimal design parameters to satisfy gas consumption rate, carbon monoxide emission, and heating performance. Optimal design values were established to be a nozzle diameter of 1.5 mm and a burner-pot height of 60 mm.