• Journal of Microbiology and Biotechnology
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• 제17권12호
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• pp.1930-1936
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• 2007

A sequential optimization strategy based on statistical experimental designs was employed to enhance the production of cyclosporin A (CyA) by Tolypocladium inflatum DSMZ 915 in a submerged culture. A 2-level Plackett-Burman design was used to screen the bioprocess parameters significantly influencing CyA production. Among the 11 variables tested, sucrose, ammonium sulfate, and soluble starch were selected, owing to their significant positive effect on CyA production. A response surface methodology (RSM) involving a 3-level Box-Behnken design was adopted to acquire the best process conditions. Thus, a polynomial model was created to correlate the relationship between the three variables and the CyA yield, and the optimal combination of the major media constituents for cyclosporin A production, evaluated using the nonlinear optimization algorithm of EXCEL-Solver, was as follows (g/l): sucrose, 20; starch, 20; and ammonium sulfate, 10. The predicted optimum CyA yield was 113 mg/l, which was 2-fold the amount obtained with the basal medium. Experimental verification of the predicted model resulted in a CyA yield of 110 mg/l, representing 97% of the theoretically calculated yield.

• 한국작물학회지
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• 제40권1호
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• pp.86-91
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• 1995

벼 생력 직파재배양식으로 최근에 개발 보급된 무논골뿌림재배의 파종 피술체계확립의 일환으로 파종량에 따른 생육 및 수량반응을 검토하고자 시험을 실시하였던바, 그 결과를 요약하면 다음과 같다. 1. 파종량이 증가할수록 $m^2$당 입모수는 직선적으로 증가하였으나, 개체당 수수는 입모수 증가에 따라 완만하게 감소하는 경향이었다. 2. 유효경 비율 및 출수기 경당엽면적은 파종량이 증가함에따라 감소하였으며 출수기는 큰차이가 없었다. 3. 도복관련 형질중 간장, 수장, 좌절량, 줄기굵기 등이 파종량 증가와 함께 감소하는 경향이었고, 10a당 7kg이상에서는 포장도복이 심하게 발생되었다. 4. $m^2$당의 수수증가에 따라 수당립수와 등숙비율이 감소하였으며, 수량은 10a당 5kg을 기점으로하여 점차 감소하는 경향이었다. 5. 수량에 대한 수량구성요소의 경로계수 분석결과에 의하면 등숙비율의 직접효리가 가장컸으며, 최대수량을 얻기위한 $m^2$당 입모수는 105개 정도, 이때의 10a당 파종량은 4.5kg 수준이었다.

• 한국작물학회지
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• 제51권spc1호
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• pp.30-34
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• 2006

남부 산간고랭지에서 고품질 쌀 생산을 위한 질소시비량을 구명하고자 $2002{\sim}2004$년에 남부 산간고랭지 인 운봉에서 삼천벼를 공시하여 질소시비량에 따른 생육, 수량구성요소, 쌀 수량 및 품질 관련형질을 조사한 결과를 요약하면 다음과 같다. 1. 단위면적당 수수와 입수는 다비할수록 많았으나, 등숙비율과 현미천립중은 다비할수록 저하되었다. 2. 백미 외관상 품위에서 완전립 비율은 질소시비량이 많을수록 낮았고, 아밀로스 함량은 질소시비량 간에 별 차이가 없었으나, 단백질 함량은 질소시비량이 증가됨에 따라 다소 높아지는 경향이었다. 3. 쌀 수량과 완전미 수량은 질소 9 kg/10a 수준까지는 증수 되었으나 그 이상의 질소시비량에서는 수량 차이가 인정되지 않았다. 4. 따라서 남부 산간고랭지에서 완전미 수량, 완전미비율, 미질, 등숙비율 등을 고려한 품질향상을 위한 적정 질소시비량은 10a당 9 kg 내외로 판단된다.

• 한국토양비료학회지
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• 제47권5호
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• pp.332-339
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• 2014

This research was performed to test the hypothesis that the optimal fertilization rate for red pepper is changed by soil moisture condition. The experiment was conducted in rainfall-intercepted fields in Suwon, South Korea from 2002 to 2003. Soil was irrigated at 30, 50, or 80 kPa of soil moisture tension at 20 cm soil depth in 2002 and 30, 50, 100, or 150 kPa in 2003. For both years, fertilization was performed with four levels: none, 0.5, 1, and 1.5 times of the recommended N, P, and K fertilization rate. The irrigation amount was the greatest at 30 kPa irrigation while the water use efficiency increased with decrease of irrigation amount. The Irrigation amount was 508 mm at 30 kPa irrigation and ranged from 355 mm to 435 mm at 50 kPa irrigation. The maximum yield was found at 30 kPa irrigation and 1.5 times of the recommend fertilization rate in 2002 and 2003. The yield index of red pepper increased linearly with the fertilization rate at 30 kPa which implied that excess irrigation induced nutrient leaching and reduced nutrient availability. The maximum yield in 50 kPa and 80 kPa was found at the recommend fertilization rate while the yield decreased by fertilization at 100 kPa and 150 kPa irrigation. It implies that reduction of fertilization is the feasible practice to mitigate drought stress in fields without stable irrigation resources.

• 한국약용작물학회지
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• 제19권6호
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• pp.441-445
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• 2011

This research was conducted to investigate the influence of variouis organic substrates on growth and yield of organically grown ginseng seedlings in a shaded plastic house. In the investigation of optimal substrate, the eight substrate were formulated by adjusting blending rate of peatmoss, perlite, coir dust(coco peat), and vermiculite. Then, the changes in physico chemical properties of root substrates as well as their influences on the growth characteristics and yield were determined at six months after sowing. The elevation of the blending rate of peatmoss from 50% to 70% with decrease in the rate of inorganic component (mixture of perlite and vermiculite) from 50 to 30% resulted in the increase in container capacities and decrease in total porosities and air-filled capacities. The concentrations of $NH_4-N$, $P_2O_5$ and K increased as the incorporation rate of castor seed meal, phosphate ore, and langbenite, respectively, were elevated during the root medium formulations. The PPV-1 and PPV-4 substrates produced high stem length, stem diameter, shoot fresh weight, leaf area and root length among eight substrate. Root fresh weight was heaviest in PPV-4 compound nursery media. The results of this experiment will be utilized in the new substrate application for ginseng organic culture in shaded vinyl house.

• 한국작물학회지
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• 제38권3호
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• pp.235-239
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• 1993

모시풀 재배에 있어서의 적정 시비량을 알고자 휴지재식 초년부터 성원이된 4년째까지 시험조사한 결과를 요약하면 다음과 같다. 1. 모시풀의 경신장에는 비료 3요소중 질소, 칼리의 증시효과가 컸고, 석회도 경신장에 크게 작용하는 것으로 생각되었다. 2. 기준시비량 $P_2O_5$-$K_2$O=9-3-9kg/10a에 비하여 질소는 2～3배, 칼리는 2배비 시용에서 수량이 증대되었고, 그 중 10a당 질소 18kg, 인산 3kg, 칼리 18kg와 석회 200kg 시용의 경우 가장 유효경 비율도 높았으며 조섬유 수량에서도 33% 증수되었다.

• Korean Chemical Engineering Research
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• 제53권6호
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• pp.831-835
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• 2015

본 연구에서는 바이오매스 유래 생리활성물질인 (+)-dihydromyricetin을 정제하기 위한 분별침전공정에서 분별침전용액의 제타전위가 분별침전 양상(순도, 수율, 침전물 형태와 크기)에 미치는 영향을 조사하였다. 제타전위 조절을 위한 실리카-알루미나의 첨가량(반응액 부피당 표면적) $100mm^{-1}$에서 가장 높은 수율을 얻을 수 있었다. 실리카-알루미나의 제타전위가 양(+)의 값으로 증가할수록 (+)-dihydromyricetin 수율과 침전물의 크기는 증가하였다. 가장 큰 제타전위 값(+4.99 mV)을 가진 실리카를 이용한 분별침전의 경우에는 가장 작은 제타전위 값(-19.00 mV)을 가진 알루미나를 이용한 분별침전의 경우보다 2배 이상 높은 수율을 얻을 수 있었다. 또한 분별침전 과정에서 제타전위 절대값이 증가할수록 (+)-dihydromyricetin 수율과 침전물의 크기는 감소하여 제타전위 절대값에 반비례함을 알 수 있었다. 반면 표면적증가물질 실리카-알루미나의 제타전위 변화에도 (+)-dihydromyricetin 순도는 거의 변화가 없었다.

• 한국수산과학회지
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• 제34권4호
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• pp.359-364
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• 2001

복수어구에 의한 단일 어종 자원의 평가모델은 단일 어종에 대한 복수어구의 어획노력량이 다르므로 어업별 어획노력량을 하나의 노력단위로 표준화하여, Fox (1970)에 의한 잉여생산량모델에 적용함으로써, 최대지속적생산량, 최대경제적생산량 및 적정어획 노력량의 값을 추정하였다. 여기서는 참조기에 대한 대형기선저인 망쌍끌이어업과 근해안강망어업에 대해 적용하였고, 이 두 어업에 대한 경제성 분석을 통하여 순이익을 추정하였다. 참조기에 대한 어업별 순이익의 변동을 비교 분석한 결과 두 어업의 경제성은 근해안강망어업이 대형기선저인망쌍끌이어업 보다 더 나은 것으로 분석되었으며, 두 어업의 순이익을 같게 하기 위해서는 대형기 선저인망쌍끌이어업의 노력량을 현재보다 증가시키고 근해안강망 어업의 노력량은 낮춰야 한다.

• ALGAE
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• 제21권3호
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• pp.317-321
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• 2006

The effects pH and salinity on the brown alga Sargassum fulvellum were investigated in the context of the removal of two major associated animals, Caprella scaura and Gammaropsis utinomi. Optimum quantum yield (Fv/Fm) of S. fulvellum was also examined in the same experimental conditions as an index of stress. Experiments on pH and salinity tolerances of the two animal species indicated that mortality was more than 80% at extremes of pH (2, 3, 4, 10, 11, 12) and salinity (0, 3.5, 7, 10, 44 psu) after a 5 min treatment. Lethal time (LT50) from pH 2 to pH 4 was less than 90 sec in C. scaura, and less than 70 sec in G. utinomi. From 0 to 10 psu, LT50 was less than 20 sec in C. scaura and less than 60 sec in G. utinomi. The quantum yield of S. fulvellum was not significantly different from controls within the pH range 4~10, and within the salinity range of 7-40 psu. When exposed to pH and salinity conditions outside these ranges, the effect of these factors on the removal of two animal species was higher, but quantum yield was highly reduced. These results indicate that the optimal conditions for removing the animal species without affecting optimum quantum yield were pH 4-10, and salinities 7-10 psu and 44 psu.

• Asian-Australasian Journal of Animal Sciences
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• 제21권11호
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• pp.1592-1598
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• 2008

An experiment was carried out to study the effect of cattle slurry on maize fodder (Zea mays) production. Maize fodder was produced at 4 cattle slurry levels $T_0$ (0 ton/ha), $T_1$ (10 ton/ha), $T_2$ (12 ton/ha) and $T_3$ (14 ton/ha) in a randomized block design. Agronomic characteristics, plant heights, circumference of stems, number of leaves, leaf area and dry matter yield of maize fodder were measured. Maize plant height and stem circumference were significantly (p<0.01) influenced by the increasing rate of cattle slurry at 15, 30, 45 and 56 days after sowing. Number of leaves of fodder plants was not significant but leaf area was significant (p<0.05) among the treatment groups. The highest biomass yield (p<0.01) of maize fodder was observed in $T_2$ (44.0 ton/ha). For crude protein content, a significant difference (p<0.01) was observed in the treatment groups and the highest value was observed in $T_2$ (11.99%). Organic matter content of maize fodder showed a significant difference but ash, ADF and NDF contents showed no significant differences among treatment groups. From this study it may be concluded that the application of 12 tons of cattle slurry/ha was optimal for production of biomass and nutrient content of maize fodder.