• 제목/요약/키워드: Optimal yield

검색결과 1,480건 처리시간 0.033초

Optimal Conditions for Hepatitis B Cove Antigen Production in Shaked Flask Fermentation

  • Tey Beng Ti;Yong Kok Hoe;Ong Hong Puay;Ling Tau Chuan;Ong Swee Tin;Tan Yan Peng;Ariff Avbakariya;Tan Wen Siang
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제9권5호
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    • pp.374-378
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    • 2004
  • The effects of various environmental factors such as pH (5, 6, 7, 8 and 9), temperature (30, 37 and $40^{\circ}C$) and rotational speed (150, 200 and 250 rpm) on the growth and the hepatitis B core antigen (HBcAg) production of Escherichia coli W3110IQ were examined in the present Study. The highest growth rate is achieved at pH 7, $37^{\circ}C$ and at a rotational Speed of 250 rpm which is 0.927 $h^{-1}$. The effect of pH on cell growth is more substantial compared to other parameters; it recorded a $123\%$ different between the highest growth rate (0.927 $h^{-1}$) at pH 7 and lowest growth at pH 5. The highest protein yield is achieved at pH 9, rotational speed of 250 rpm and $40^{\circ}C$. The yield of protein at pH 7 is $154\%$ higher compared to the lowest yield achieved at pH 5. There is about $28\%$ different of the protein yield for the E. coli cultivated at 250 rpm compared to that at 150 rpm which has the lowest HBcAg yield. The yield of protein at $40^{\circ}C$ is $38\%$ higher compared to the lowest yield achieved at $30^{\circ}C$.

High-Yield Production of Xylitol from Xylose by a Xylitol Dehydrogenase Defective Mutant of Pichia stipitis

  • Kim, Min-Soo;Chung, Yun-Seung;Seo, Jin-Ho;Jo, Do-Hyun;Park, Yun-Hee;Ryu, Yeon-Woo
    • Journal of Microbiology and Biotechnology
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    • 제11권4호
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    • pp.564-569
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    • 2001
  • This study was carried out in order to investigate the characteristics of xylitol fermentation by a xylitol dehydrogenase defective mutant PXM-4 of P stipitis CBS 5776 and to determime optimum conditions for the high yield ofxylitol production from xylose. Gluconic acid was selected as a co substrate for the xylitol fermentation, since gluconic acid neither blocked xylose transport nor repressed xylose reductase expression. An increase of gluconic acid concentration reduced the rates of xylitol production and cell growth by decreasing medium pH, and the optimal concentration of gluconic acid was determined to be 20 gll with approximately 100% xylitol conversion yield. A fed-batch cell culture resulted in a 44.8 g/l xylitol concentration with 100% yield, based on the amount of xylose consumed.

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산 가수분해와 발효에 의한 해조류로부터 에탄올 생산 (Ethanol Production From Seaweeds by Acid-Hydolysis and Fermentation)

  • 나춘기;송명기;손창인
    • 신재생에너지
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    • 제7권3호
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    • pp.6-16
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    • 2011
  • In order to study the utilization of seaweeds as an alternative renewable feedstock for bioethanol production, their properties of hydrolysis and fermentation were investigated. The seaweeds were well hydrolyzed with diluted sulfuric acid. The weight loss of seaweeds reached 75-90%, but only 12-51% of them was converted into reducing sugars after the acid-hydrolysis at $130^{\circ}C$ for 4-6h. The yield of reducing sugars increased with increasing the hydrolysis time up to 4h and then decreased thereafter. In contrast, the ethanol yield from the hydrolysates increased with hydrolysis time except for green seaweeds maximizing at 4h. Optimal fermentation time by Saccharomyces cerevisiae (ATCC 24858) varied with seaweeds; 48h for green seaweeds, 96h for brown and red seaweeds. The ethanol yield from the hydrolysate reached 138${\pm}$37mg/g-dry for green seaweeds, 258${\pm}$29mg/g-dry for brown seaweeds, and 343${\pm}$53mg/g-dry for red seaweeds, which correspond to approximately 1.5-4.0 times more than the theoretical yield from total reducing sugars in the hydrolysates. The results obtained indicate clearly that the non-reducing sugars or oligosaccharides dissolved in the hydrolysate played an important role in producing bioethanol. Considering the productivity and production cost of each seaweed, brown seaweeds such as Laminaria japonica and Undaria pinnatifida seem to be a promissing feedstock for bioethanol production.

더덕순 채취가 수량 및 품질에 미치는 영향 (Effects of Pinching times on Root Yield and Quality of Codonopsis lanceolata (S.et Z.) Trautv.)

  • 최시동
    • 한국자원식물학회지
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    • 제9권3호
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    • pp.268-275
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    • 1996
  • 봄철 더덕순 채취(採取)가 더덕의 수량(收量) 및 품질(品質)에 미치는 영향(影響)을 구명(究明)하기 위하여 1992년부터 1994년까지 3년간 시험한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 더덕순 채취별(採取別) 생육(生育)은 채취회수(採取回數)가 많을수록 생육은 부진하였으며, 종자에서도 중량(重量)이 가벼워지는 경향이었다. 2. 더덕순 채취수량(採取收量)은 3회(回) 채취구(採取區)에서 3년간 29.5MT/ha으로 가장 높았고, 가식률(可食率)은 1회 채취구(採取區)가 100%로 가장 높게 나타났다. 3. 더덕뿌리에 있어서 더덕순 채취(採取)는 무채취(無採取)에 비(比)하여 오히려 품질(品質) 및 수량(收量)의 감소(減少)를 나타내었으며, 채취가능(採取可能) 회수(回數)는 1회(回) 채취(採取)정도였고 그 이상(以上)은 수량(收量) 및 품질저하(品質低下)로 채취(채취)가 불가능하였다.

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Effect of Plant Density on Growth and Sugar Yield of Sweet Sorghum in Jeju Island

  • Oh, Hyeon-Do;Kim, Han-Lim;Kang, Young-Kil;Kim, Chan-Woo
    • 한국작물학회지
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    • 제46권1호
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    • pp.29-34
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    • 2001
  • A sweet sorghum [Sorghum bicolor (L.) Moench] cultivar was planted on 9 and 30 June 2000 at plant densities of 4, 6, 8, 10, and 12 plants $m^{-2}$ to determine the optimum plant density in Jeju region. There were no significant planting date x plant density interactions for most traits measured. Delaying planting from 9 to 30 June delayed 21 days in heading date, and significantly decreased plant height, the number of productive stems $m^{-2}$/, and lodging. Fresh stem yield tended to be higher at the 9 June planting date than at the 30 June planting date, but total sugar and ethanol yields were not significantly affected by planting date. Percentage of soluble solid was higher at the 30 June planting date compared with the 9 June planting date. Fresh stem, total sugar, and ethanol yields quadractically increased from 22.9 to 36.7 $t^{-1}$, from 1.66 to 2.54 $t^{-1}$, and from 945 to 1440 $L^{-1}$, respectively, with increasing plant density. The optimal plant densities for the maximum fresh stem, total sugar, and ethanol were estimated to be 10.7, 9.6, and 9.9 plants $m^{-2}$ respectively.

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가압형 마이크로웨이브 추출장치를 이용한 가용성 인삼성분의 추출조건 최적화 (Optimization of Extraction Conditions for Soluble Ginseng Components Using Microwave Extraction System under Pressure)

  • 권중호;이새봄;이기동
    • 한국식품영양과학회지
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    • 제28권2호
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    • pp.409-416
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    • 1999
  • Microwave extraction system equipped with closed vessels was applied to confirm its capability of extracting ginseng solubles including saponin, which was monitored to optimize extraction con ditions by response surface methodology. Total yield increased with the decrease in particles size and more extraction steps. Soluble components were completely extracted by operating the system within 6 min per one step, which should be repeated by 3 or 4 times. Optimized conditions for maxi mum extraction of response variables(total yield, crude saponin) were 40~50% of ethanol concentration, about 140oC of extraction temperature, and within 6 min of the extraction time. The extraction effi ciency of total yield was dependent on the decrease of ethanol concentration, while crude saponin content was favored on the higher degree of ethanol concentration. According to the temperature guideline, below 90oC of extraction temperature, the optimal ranges of extraction conditions were predicted as 30~50% of ethanol concentration and 2.5~6.5 min of extraction time. Estimated values of total yield and crude saponin were in good agreement with experimental values.

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Changes of Anthocyanidin, Growth Characteristics and Brown Rice Yield of Red Colored Rice at Two Region of Eastern Coast in Gyeongsangbuk-do Province

  • Kim, Sang-Kuk;Kim, Se-Jong;Shin, Jong-Hee
    • 한국자원식물학회지
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    • 제30권3호
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    • pp.318-322
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    • 2017
  • The study was carried out to determine the changes of growth characteristics, anthocyanidin, and brown rice yield grown at coast and inland areas apart from the east coastline of Gyeongsangbuk-do province. Number of spikelets per panicle was much more increased in inland area. Regional difference in number of spikelets per panicle was observed in Jeongjinju cultivar. Among these red rice cultivars, the highest brown rice yield was Jeongjinju rice cultivar having 702 kg in inland area and 692 ㎏ in coast area, respectively. Anthocyanidin content ranged 524 to $610{\mu}g/g$ dry weight basis. Cyanidin content was 11.4 to 14.0 times higher than that of delphinidin under coast and inland area. Anthocyanidin content was higher in rice cultivar grown at coast area. Highest head brown rice rate was only observed in Geonganghongmi to 95.2 at coast area and 95.4 inland area. In considering brown rice yield and pigment content, Hongjinju rice cultivar was recommended in optimal pigment rice cultivar in eastern coast of Gyeongsangbuk-do Province.

Multiscale Simulation of Yield Strength in Reduced-Activation Ferritic/Martensitic Steel

  • Wang, Chenchong;Zhang, Chi;Yang, Zhigang;Zhao, Jijun
    • Nuclear Engineering and Technology
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    • 제49권3호
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    • pp.569-575
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    • 2017
  • One of the important requirements for the application of reduced-activation ferritic/martensitic (RAFM) steel is to retain proper mechanical properties under irradiation and high-temperature conditions. To simulate the yield strength and stress-strain curve of steels during high-temperature and irradiation conditions, a multiscale simulation method consisting of both microstructure and strengthening simulations was established. The simulation results of microstructure parameters were added to a superposition strengthening model, which consisted of constitutive models of different strengthening methods. Based on the simulation results, the strength contribution for different strengthening methods at both room temperature and high-temperature conditions was analyzed. The simulation results of the yield strength in irradiation and high-temperature conditions were mainly consistent with the experimental results. The optimal application field of this multiscale model was 9Cr series (7-9 wt.%Cr) RAFM steels in a condition characterized by 0.1-5 dpa (or 0 dpa) and a temperature range of $25-500^{\circ}C$.