• Journal of Microbiology and Biotechnology
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• 제20권11호
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• pp.1534-1538
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• 2010

Fed-batch cultures of Hansenula polymorpha were studied to develop an efficient biosystem to produce recombinant human serum albumin (HSA). To comply with this purpose, we used a high-purity oxygen-supplying strategy to increase the viable cell density in a bioreactor and enhance the production of target protein. A mutant strain, H. polymorpha GOT7, was utilized in this study as a host strain in both 5-l and 30-l scale fermentors. To supply high-purity oxygen into a bioreactor, nearly 100% high-purity oxygen from a commercial bomb or higher than 93% oxygen available in situ from a pressure swing adsorption (PSA) oxygen generator was employed. Under the optimal fermentation of H. polymorpha with highpurity oxygen, the final cell densities and produced HSA concentrations were 24.6 g/l and 5.1 g/l in the 5-l fermentor, and 24.8 g/l and 4.5 g/l in the 30-l fermentor, respectively. These were about 2-10 times higher than those obtained in air-based fed-batch fermentations. The discrepancies between the 5-l and 30-l fermentors with air supply were presumably due to the higher contribution of surface aeration over submerged aeration in the 5-l fermentor. This study, therefore, proved the positive effect of high-purity oxygen in enhancing viable cell density as well as target recombinant protein production in microbial fermentations.

• Journal of Biosystems Engineering
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• 제42권1호
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• pp.23-43
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• 2017

Purpose: As application-specific wireless sensor networks are gaining popularity, this paper discusses the development and field performance of the GHAN, a greenhouse area network system to monitor, control, and access greenhouse microenvironments. GHAN, which is an upgraded system, has many new functions. It is an intelligent wireless sensor and actuator network (WSAN) system for next-generation greenhouses, which enhances the state of the art of greenhouse automation systems and helps growers by providing them valuable information not available otherwise. Apart from providing online spatial and temporal monitoring of the greenhouse microclimate, GHAN has a modified vapor pressure deficit (VPD) fuzzy controller with an adaptive-selective mechanism that provides better control of the greenhouse crop VPD with energy optimization. Using the latest soil-matrix potential sensors, the GHAN system also ascertains when, where, and how much to irrigate and spatially manages the irrigation schedule within the greenhouse grids. Further, given the need to understand the microclimate control dynamics of a greenhouse during the crop season or a specific time, a statistical assessment tool to estimate the degree of optimality and spatial variability is proposed and implemented. Methods: Apart from the development work, the system was field-tested in a commercial greenhouse situated in the region of Punjab, India, under different outside weather conditions for a long period of time. Conclusions: Day results of the greenhouse microclimate control dynamics were recorded and analyzed, and they proved the successful operation of the system in keeping the greenhouse climate optimal and uniform most of the time, with high control performance.

• 에너지공학
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• 제21권2호
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• pp.144-151
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• 2012

지역난방시스템이란 열에너지를 생산하고 만들어진 열에너지를 넓은 지역에 공급하는 시스템이다. 지역난방시스템은 국부난방보다 더 효율적이고 친환경적이라고 할 수 있다. 생산 된 열은 단열 배관 네트워크를 통해 사용자들에게 공급된다. 지역난방시스템의 효율적인 운전을 위해서는 여러 가지 운전 조건에서 배관망 네트워크의 유량, 압력 및 온도 분포를 예측하는 것이 중요하다. 본 연구에서는 지역난방용 지중매설 배관망 네트워크에서의 열-유체 동적거동을 수학적 모델을 사용하여 예측하였다. 수학적 모델은 물질, 운동량 및 에너지 수지식을 사용하였다. 모델링의 결과를 검증하기 위하여 강남지역 지역난방시스템에서 측정한 결과와 모델링의 결과를 비교하였다.

• 한국가시화정보학회지
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• 제18권2호
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• pp.59-65
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• 2020

Vertical axis wind energy systems including 3 and 4 blades are numerically investigated in a two-dimensional (2D) computational domain. The power coefficient (C_p) is adopted to measure the efficiency of the system and the effect of the rotating velocity on the power coefficient is analyzed for the two different systems. The rotating velocity varies from 30 rad/s to 90 rad/s, which corresponds to the tip speed ratio (T.S.R) of 0.5 to 1.5. The torque exerted on the blades is mainly determined by the aerodynamic force in the x-direction and maximized when the blade is positioned at around θ = 186°. The efficiency of the 4-blade system is higher than that of the 3-blade system within the tip speed ratio range between 0.5 and 0.67, besides where the 3-blade system shows a better performance. For the 3-blade system, the maximum efficiency is reached to 0.082 at the tip speed ratio of 1.083. The maximum efficiency of the 4-blade system is 0.071 at T.S.R. = 0.92. The velocity fields in the x-direction, pressure fields, and the vorticity magnitude are analyzed in detail for the optimal cases of the 3- and 4-blades systems, respectively.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.371-372
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• 2012

We have studied lead-based gapless semiconductors, $PbPdO_2$, which is very sensitive to external parameters such as temperature, pressure, electric field, etc[1]. We have fabricated pure $PbPdO_2$, Co- and Mn-doped $PbPdO_2$ thin films using the pulsed laser deposition. Because of the volatile element of Pb, it is very difficult to grow the films. Note that in case of $MgB_2$, Mg is also volatile element. So in order to enhance the quality of $MgB_2$, some experiments are carried out in annealing with Mg-rich atmosphere [2]. This annealing process with volatile element plays an important role in making smooth surface. Thus, we applied such process to our studies of $PbPdO_2$ thin films. As a result, we found the optimal condition of ex-situ annealing temperature ${\sim}650^{\circ}C$ and time ~12 hrs. The ex-situ annealing brought the extreme change of surface morphology of thin films. After ex-situ annealing with PbO-rich atmosphere, the grain size of thin film was almost 100 times enlarged for all the thin films and also the PbO impurity phase was smeared out. And from X-ray diffraction measurements, we determined highly crystallized phases after annealing. So, we measured electrical and magnetic properties. Because of reduced grain boundary, the resistivity of ex-situ annealed samples changed smaller than no ex-situ sample. And the carrier densities of thin films were decreased with ex-situ annealing time. In this case, oxygen vacancies were removed by ex-situ annealing. Furthermore, we will discuss the transport and magnetic properties in pure $PbPdO_2$, Co- and Mn-doped $PbPdO_2$ thin films in detail.

• 한국의류학회지
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• 제9권3호
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• pp.45-51
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• 1985

The purpose of this study is to research into the thermal condition and the weight of clothes suitable for the officers engagged in light works in an air-conditioned room in summer. The Major findings are as follows: 1. Thermal conditions of the working environment are $24.5^{\circ}C$ (Dry bulb temp.), $68\%$ (Relative humidity) and 2.6m/sec (Air Velocity). 2. Total clothing weights are 416.6 g/$m^2$ (male) and 340.9 g/$m^2$ (female). Underwear weights are 96.8g/$m^2$ (male) and 85.1g/$m^2$ (female). The latter turned out to be statistically significant in Sexual difference. 3. Means of the thermal sensation are 3.0 (comfortable; male) 2.7 ('Slightly cool' -'Comfortable'; female) and the relationship between clothing weights and thermal sensation proves to be significantly correlated in the case of female. 4. $66.7\%$ of the women and $37.1\%$ of the men feel sensation of coldness at the body's specific area and $79.5\%$ of the women and $54.3\%$ of the men reveals air-conditioning disturbance. 5. As the thermal sensation is close to 'cool-cold', sensation of coldness or air conditioning disturbance are showed up more frequently which is reversely related with weight of under-wear. It is also proved that air conditioning disturbances are influenced by sexual difference in addition to sensation of coldness and thermal sensation. 6. According to the result of experiment, we can have the idea that at condition $X_1$, the drop of limbs' skin temp. is remarkable. At condition $X_2$ skin temp. for distal limbs and mean skin temp. are raised and the falling degree is similar. At condition $X_3$, mean skin temp. and distal skin temp. are remarkably raised, and the falling degree decreases and the beats of pulse rate increase and diastoric blood pressure is lowered.

• 디지털콘텐츠학회 논문지
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• 제16권3호
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• pp.355-363
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• 2015

본 논문에서는 ATmega기반의 스마트 제어보드를 이용하여, 지형의 경사로에 따라 실시간으로 전동휠체어의 시트를 제어하는 시스템을 제안한다. 스마트 제어보드에는 자이로 및 가속도 센서와 Tilt센서가 포함되어 있으며, 전동휠체어가 다양한 경사로 지형을 지나갈 때 실시간으로 2개의 센서를 이용하여, 지형을 파악할 수 있도록 구성되었다. 본 논문에서는 정확한 지형을 파악할 수 있도록 자이로 센서와 가속도 센서 기반에 상호보안 필터를 적용하여, 전동휠체어가 이동 중에 출력되는 센서 값의 노이즈를 해결하였다. 이를 기반으로 전동휠체어가 이동시 센서 출력 값의 노이즈로 인하여, 시트가 지속적으로 흔들리지 않고 안정적으로 시트 평행을 유지하는 것을 확인할 수 있다. 본 논문에서는 사용자의 편의를 위해 전동휠체어에 익숙하지 않은 사람들이 쉽게 제어할 수 있도록 스마트 폰 기반의 제어 플랫폼을 제공한다. 스마트 폰의 제어플랫폼은 전동휠체어의 현재 상태를 실시간으로 모니터링 할 수 있으며, 욕창 방지와 관련하여, 시트의 기울기를 임의적으로 제어할 수 있도록 도와준다.

• Safety and Health at Work
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• 제3권4호
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• pp.287-293
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• 2012

Objectives: Metabolic syndrome has received attention as a risk factor for cardiovascular disease, with particular importance attached to visceral fat accumulation, which is associated with lifestyle-related diseases and is strongly correlated with waist circumference. In this study, our aim is to propose waist circumference cut-off values that can be used as a marker for fatty liver based on a sample of workers receiving health checkups in Japan. Methods: This study was conducted in a total of 21,866 workers who underwent periodic health checkups between January 2007 and December 2007. The mean age of the subjects was 47.4 years for men (standard deviation [SD]: 8.0) and 44.7 years for women (SD: 6.9). Evaluation included abdominal ultrasound and measurement of waist circumference, body mass index, fasting blood glucose, triglycerides, high-density lipoprotein cholesterol, and blood pressure. Results: Based on receiver operating characteristic curve analysis, the optimal waist circumference cut-off values were shown as 85.0 cm (sensitivity 0.72, specificity 0.69) for men and 80.0 cm (sensitivity 0.75, specificity 0.78) for women. Conclusion: Abdominal ultrasound is the most efficient means of diagnosing fatty liver, but this examination seldom occurs because the test is not routinely performed at workers' health checkups. In people found to have a high risk of fatty liver, recommendations can be made for abdominal ultrasound based on the waist circumference cut-off values obtained in this study. That is, waist circumference can be used in high risk individuals as an effective marker for early detection of fatty liver.

• International Journal of Naval Architecture and Ocean Engineering
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• 제3권4호
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• pp.274-285
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• 2011

The supercavitating vehicle is an underwater vehicle that is surrounded almost completely by a supercavity to reduce hydrodynamic drag substantially. Since the cruise speed of the vehicle is much higher than that of conventional submarines, the drag force is huge and a buckling may occur. The buckling phenomenon is analyzed in this study through static and dynamic approaches. Critical buckling load and pressure as well as buckling mode shapes are calculated using static buckling analysis and a stability map is obtained from dynamic buckling analysis. When the finite element method (FEM) is used for the buckling analysis, the solver requires a linear static solver and an eigenvalue solver. In this study, these two solvers are integrated and a consolidated buckling analysis module is constructed. Furthermore, Particle Swarm Optimization (PSO) algorithm is combined in the buckling analysis module to perform a design optimization computation of a simplified supercavitating vehicle. The simplified configuration includes cylindrical shell structure with three stiffeners. The target for the design optimization process is to minimize total weight while maintaining the given structure buckling-free.

• 대한화장품학회지
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• 제24권3호
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• pp.116-122
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• 1998

It is well known that all-trans-retinol is not only very unstable in heat, light, air, and water, but also skin-irritant despite a good anti-wrinkle effect. Therefore, it is very difficult to stabilize retinol and make the safe retinol containing cosmetics by using a certain concentration of retinol with real effect. In order to dissolve these problems and apply retinol for skin care cream, firstly retinol is to be encapsulated in the vesicle called Liposphere (pseudo-liposome) which is made by homogenizing under high pressure the mixtures of lecithin, retinol, caprylic/capric triglyceride, and hydroalcoholic solution ; and then this retinol containing Liposphere is to be intercalated in lamellar liquid crystal layer which is prepared by emulsifying in an optimal ratio the mixtures composed of non-ionic emulsifier (cetearyl glucoside, sorbitan stearate & sucrose cocoate etc), cetearyl alcohol, stearic acid, cholesterol, and ceramide. In addition, the stability of the retinol containing oil in water cream by adding the polymeric emulsifier such as acrylate /C10-30 alkyl alkylate crosspolymer is to be ensured even at 55 C. Retinol containing oil in water cream prepared through above procedure could be very stable at 45 C for at least 50 days. The structure identification of lamellar liquid crystal was determined using polarized light microscope and electron microscope Conclusively, we could make the very stable retinol containing oil in water cream by triple procedure, that is, encapsulation of retinol in Liposphere, intercalation of retinol in lamellar liquid crystal layer, and assurance of the high temperature stability of cream even at 55 C.