• Microbiology and Biotechnology Letters
• /
• v.24 no.6
• /
• pp.699-704
• /
• 1996

About 600 microorganisms isolated from soil, marsh, compost, etc. were examined for their flocculating ability in the kaolin suspension and swine wastewater. Among them, K-199 was the best producer of flocculant and was identified to be a species belong to the genus Corynebacterium. The maximum production of the flocculant from Corynebacterium sp. K-199 was observed in culture medium containing 2% glycerol, 0.4% peptone, 0.3% K$_{2}$HPO$_{4}$, 0.1% CaCO$_{3}$, 0.05% NaCl, 0.1% yeast extract, 0.1% MgSO$_{4}$,7H$_{2}$O and initial pH 7.5 when cultured with rotary shaker controlled at 25$\circ$C and 150 rpm. Under the optimal culture condition with jar fermentor, the maximum production was reached to flocculating activity of 780 units/ml after 4 days. From the results of the activity be fully maintained by periodate oxidization, it suggests that the activity is due to the protein.

• Microbiology and Biotechnology Letters
• /
• v.23 no.5
• /
• pp.531-535
• /
• 1995

The identification of eicosapentaenoic acid (EPA) by Pseudomonas sp. CH-414 were investigated under the optimal culture conditions. The maximum dry cell weight and phospholipid production showed about 10 mg/ml and 0.6 mg/ml, respectively, at the 48 hr cultivation. The phospholipid form produced by Pseudomonas sp. CH-414 was elucidated as a phosphatidyt ethanolamine by thin layer chromatography. EPA was contained about 15% in the. extractable lipid, and the amount of EPA was about 83 $\mu $g/ml from the culture broth. Also myristic, palmitic, palmitoleic, stearic and oleic acids were identified with gas chromatography.

• Journal of Microbiology and Biotechnology
• /
• v.1 no.4
• /
• pp.288-292
• /
• 1991

The aim of the current study is to evaluate the effects of medium compositions on the production of protease inhibitor in Streptomyces sp. SMF13. The production of protease inhibitor was counter-currently linked to extra-cellular protease, which were regulated by the culture conditions. Nitrogen source was the most critical ingredient affecting the production of protease inhibitor and protease. Carbon source was an important factor to determine the culture pH which affected very clearly the formation of protease and protease inhibitor. Inorganic phosphate inhibited the protease inhibitor production which was linked to the cell growth rate, although the optimal conditions for the production of protease inhibitor were not favouring to the cell growth.

• Korean Journal of Plant Resources
• /
• v.1 no.1
• /
• pp.88-92
• /
• 1988

The study was conducted to determine the optimal basal media and the concentration of plant growth regulator for germination of seeds and growth of plantlet from Dendrobium monile. The results obtained were summarized as follows. Germination was similar in light and dark condition, but the growth of plantlet after germination was better under dark than under light condition in several media. Germination was best in Hyponex and Kyoto solution medium among the 9 media tested. The number of roots/shoot was most in the Hyponex medium containing 0,1ppm NAA and 1.Oppm BA.

• International Journal of Advanced Culture Technology
• /
• v.8 no.3
• /
• pp.211-215
• /
• 2020

Currently, most of the chlorine dioxide gas is processed at the beginning of storage or distribution. It has the disadvantage of not being able to continuously process gas since there is no system that can continuously process it during the distribution process. Therefore, in order to minimize changes in freshness and quality during the distribution process of agrifood, there is a need for a sustained-release chlorine dioxide gas treatment technology that can be continuously released. Therefore, in this study, the film to be used was examined so that the chlorine dioxide gas can be continuously released for a certain period of time, the concentration of the reactant and the viscosity at the time of the reaction were determined, and a chlorine dioxide gas gel pack was manufactured using this optimal condition. In addition, the gel pack was used to measure the amount of chlorine dioxide gas released and the sterilization effect of food poisoning bacteria.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2003.09a
• /
• pp.326-329
• /
• 2003

When an electrokinetic process is applied to a HOC-contaminated soil, hybrid types combined with soil flushing, chemical oxidation, and bioremediation are generally used. Especially when the electrokinetic process is combined with bioremediation, the hybrid technology can solve several limits of bioremediation such as low microbial mobility, low soil temperature, and shortage of nutrients in subsurface circumstance. Because microbial surface is charged negatively, the microorganism moves from cathode to anode under electrical field. In this study, mixed culture mainly-consisted by Pseudomonas sp. was applied to remediate pentadecane-contaminated kaolinite with particle size less than 300${\mu}{\textrm}{m}$. This remediation system was named ‘electrokinetic bioaugmentation’ and consisted of model aquifer, electrode reservoirs, bioreactor, power supply, and pump. The mixed culture above 0.5 of optical density in bioreactor was supplied to two reservoirs and penetrated soil when the electric current was applied. To enhance the removal efficiency, the optimal medium composition, electric current, and voltage were investigated.

• The Journal of the Korean Society for Microbiology
• /
• v.16 no.1
• /
• pp.65-70
• /
• 1981

A microculture technique for the mixed lymphocyte culture test(MLC) in the sheep is described. The optimal incubation periods for the MLC are varied from 4 to 6 days depending on the sources of lymphocytes. It is found that a 2.5:1 stimulator-responder cell ratio and the responder cell concentration of $1{\times}10^5$ cells/well produce the highest($^3H$)-thymidine incorporation. Moreover cryopreservation of bovine lymphocytes results in satisfactory and reproducible MLC reaction. It is also evident that the MLC reactions of the sheep are under the control of histocompatibility matching between the stimulator cells and the responder cells. Significance of the technique as a too! for study on cell mediated immunity in sheep system, either normal or squamous cell carcinoma-bearing, are discussed.

• International Journal of Advanced Culture Technology
• /
• v.5 no.3
• /
• pp.40-45
• /
• 2017

This data mining technique was used to extract useful information from percutaneous coronary intervention data obtained from the US public data homepage. The experiment was performed by extracting data on the area, frequency of operation, and the number of deaths. It led us to finding of meaningful correlations, patterns, and trends using various algorithms, pattern techniques, and statistical techniques. In this paper, information is obtained through efficient decision tree and cluster analysis in predicting the incidence of percutaneous coronary intervention and mortality. In the cluster analysis, EM algorithm was used to evaluate the suitability of the algorithm for each situation based on performance tests and verification of results. In the cluster analysis, the experimental data were classified using the EM algorithm, and we evaluated which models are more effective in comparing functions. Using data mining technique, it was identified which areas had effective treatment techniques and which areas were vulnerable, and we can predict the frequency and mortality of percutaneous coronary intervention for heart disease.

• Microbiology and Biotechnology Letters
• /
• v.23 no.3
• /
• pp.297-303
• /
• 1995

Antitumor antibiotic material was produced by Streptomyces sp. YBE-316 which was isolated from soil, and the optimal culture conditions for the antitumor antibiotic material production were as follows; 2.0% (w/v) sucrose, 0.8% (w/v) polypeptone, 0.4% (w/v) yeast extract, 0.2% (w/v) K$_{2}$HPO$_{4}$, pH 7.0, at 30$\circ$C, 150 rpm and for 100 hours culture. The antitumor antibiotic material had strong antitumor antibiotic activities against most testing tumor cell lines, gram positive and negative bacteria, yeasts, and, especially, Penicillium chrysogenum in fungi.

• International Journal of Advanced Culture Technology
• /
• v.4 no.3
• /
• pp.48-55
• /
• 2016

Conventional techniques for solving the noise problem have problems to generate different results, depending on the image size and weight values of the used masks, and they require many operations by using a complex formula. In this paper, we propose an image enhancement algorithm to solve the noise problem in a simple, yet easy-to-use way. For this purpose, we determined the difference between the noise of the two adjacent pixels for the horizontal and vertical, and for the two diagonal directions that each of the noise problem occurred, and then we got the average value of these pixel values. Then, we solve the noise problem by using the optimal average value in accordance with occurrence of the noise in the horizontal and vertical, and two adjacent pixels in a diagonal direction. As a result, we got the result that the noise solution in a simple, yet easy-to-use method to obtain a resultant image.