• Asian Journal of Innovation and Policy
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• 제5권1호
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• pp.78-91
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• 2016

To counter-attack against piracy, the movie industry is continuously developing new technologies for the protection of intellectual properties, only to find them instantly useless especially in the digital age. This study shifts the focus from technology to customer behavior, and analyzes customer behaviors vis-à-vis piracy using economic models. The theoretical model of optimal holdback strategy under the threat of piracy was derived and the result shows that holdback can be used as a tool not only for hedging the loss due to piracy, but also for reducing piracy. Based on the theoretical model, we suggested proper holdback strategy for each type of movie piracy.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.594-597
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• 2001

RhodopseudolllOllas palustris P4 was studied for $H_2$ production from glucose in batch culture. Important conditions studied include phosphate concentration, initial pH, temperature, glucose concentration, and gas-phase replacement. Optimal $H_2$ production was observed at 60 - 300 mM of phosphate and 7.8 - 8.6 of initial pH. The effect of culture temperature was negligible When glucose concentration increased from 0.1 to 5% (w/v), $H_2$ production increased up to 2% and remained constant thereafter. Intermittent purging of the reaction botlle with Ar gas stimulated the Hl production by alleviating the inhibition by $H_2$. The maximum productivity was 111.1 ml $H_2$/h-1.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.640-641
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• 2001

The mycelium of 3 edible mushrooms, Shizophyllum commune, pleurotus ostreatus, Tricholoma sp. was cultured in a liquid for the production of a fibrinolytic enzyme. Among them, Shizophyllum commune produced highest amount of the enzyme. The intramycelial production of the fibrinolytic enzyme by the culture of the Shizophyllum commune mycelium was approximately 6-fold higher than the extramycelial production. The carbon and nitrogen sources for the production of the enzyme were 1.5% corn steep liguor and 1.0% soytone, respectively, and optimal culture temperature and initial pH were $25^{\circ}C$ and pH 6, respectively.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.249-252
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• 2003

Maximal production of heteropolysaccharide-7(PS-7) with a batch culture for 48 hr was $10.0\;g/{\ell}$ and its conversion rate from 2% (w/v) glucose to PS-7 was 50%. After substitution of media, production of PS-7 continued and reached its maximal production. The highest productivity occurred when the fresh medium, which contained all ingredients, for the production of PS-7 was substituted. Higher production of PS-7 was maintained at a dilution rate of 0.0125, which was established as the optimal dilution rate for the production of PS-7 by B. indica HS-2001.

• 환경위생공학
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• 제15권1호
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• pp.34-38
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• 2000

In the screening of phenol-degrading bacteria, a strain showing good growth in media containing phenol was isolated by using enrichment culture from various sample and identified as genus Klebsiella pneumoniae. The optimal temperature and pH for cell growth of Klebsiella pneumoniae was $35^{\circ}C$ and 8.0, respectively. When phenol was added to the minimal media as a sole source of carbon and energy, the concentration of maximum and optimum for cell growth was 1,200ppm and 1,000ppm, respectively. It was observed that Klebsiella pneumoniae was able to degrade 98% of phenol (1,000ppm) after 40hr in culture. The isolated could utilize various kinds of aromatic compounds and showed good growth in presence of phenol, m-cresol and 3-methyl catechol.

• 한국약용작물학회지
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• 제12권6호
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• pp.448-453
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• 2004

Transformed roots of Schisandra chinensis were obtained following co-cultivation of in vitro cultivated plantlet segments with Agrobaterium rhizogens ATCC15834. This root was examined for its growth and gomisin J contents under various culture conditions. Among the six basal culture media tested, WPM (Lloyd & McCown, 1980) medium supplemented with 5% sucrose was the best roots growth 6.2 (g D.W/flask) and gomisin J accumulation 1.56 $(X10^{-3}\;ug/g\;D.W)$. Initial inoculum size correlated with the yield of biomass while gomisin J contents was not affect. Gomisin J production was influenced by the initial sucrose concentration and the highest production yield was achieved at the concentration of 7%. The optimal shaking speeds for roots growth and gomisin J production was 120 and 140 rpm, respectively.

• 한국환경과학회지
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• 제8권4호
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• pp.443-449
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• 1999

This paper describe the working of algal culture system under batch and continuous feeding effluents in biological treatment process. The main objective of this study was the determination of fundamental opeating parameters such as dilution rates, light intensity, biomass concentration, nutrients contents, which engender an effective nutrient and organic waste removal process. The results of this research indicate that the algae system will remove effectively nutrient and organic waste. In batch cultures, 91.8% dissolved orthophosphate and 83.3% ammonia nitrogen were removed from the sewage in ten days. In continuous flow systems, a detention time of 2.5 days was found adequate to remove 91% T-P, 87% T-N and 95% $NH_3-N$. At 22-28$^{\circ}C$, 60 rpm, with an intensity of 3500 Lux, the specific growth rate, k was 0.59/day in batch experiments. The optimal growth temperature and nutrients rate (N/P) were respectively $25^{\circ}C$ and 3~5. With an abundant supply of untrients, it was possible to sustain substantial population densities in the temperature range of 22~28$^{\circ}C$.

• 한국식물병리학회지
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• 제10권3호
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• pp.215-221
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• 1994

Polygalacturonase (PG) produced by Botrytis cinerea in the culture broth containing citrus pectin as a carbon source was partially purified and characterized. PG was produced on a range of carbon sources such as starch, glycerol, cellobiose, and Na+-PAG with total activities of 34.8, 32.0, 29.2, 27.8 units, respectively. The specific activity was highest with 2316.7 units on Na+-PGA. Proteins of culture filtrate were concentrated with polyethylene glycol and acetone and applied to a hydroxyapatite column. Among three active fractions collected from the column, the reaction containing the highest PG activity was resolved by a Q-sepharose column. The active fraction from the Q-sepharose column was further purified by HPLC Mono Q column. The partially purified enzyme was analyzed by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Among a few protein bands revealed, the amount of the protein of which molecular weight estimated to be 43 kDa coincided with the PG activity. The partially purified PG had optimal temperatures between 35~55$^{\circ}C$ and pH between 4.5~5.5.

• 한국미생물·생명공학회지
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• 제28권3호
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• pp.156-160
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• 2000

국내 부존자원인 inulin을 이용하여 fructose syrup 또는 oligosacharide를 생산한는 효소를 발굴하기 위하여 전국적으로 토양 등 시료를 채취하여 inulinase 생성 미생물을 분리하였으며 inulinase 활성이 높은 96-1 균주를 동정한 결과 그람 양성균인 Arthrobacter protophormiae/ramosus인 것으로 잠정 동정되었다 Inulinase 생산을 위한 최적 배지는 무기염류 기초배지에 각 1%(w/v)의 inulin tryptone. $NH_4Cl$이 함유된 배지였으며 퇴적 발효조건은 $35^{\circ}C$초기 pH 7.5 통기량 1vvm 교반속도 200rpm이었다.

• 한국미생물·생명공학회지
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• 제25권6호
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• pp.606-611
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• 1997

Entomogenous fungi which attack living insects are powerful means for microbiological insecticide. The purpose of this study is to establish the culture conditions and media for mass production of Beauveria sp. C208 which has a broad host range as a potential microbiological pesticide. The temperature and pH range for optimal cultivation of this strain were 28$circ$C and pH 5.0-7.0. For Beauveria sp. C 208, 2% rice straw and 0.6% tryptone were found as the proper carbon and nitrogen sources, considering cell mass, enzyme activities such as chitinase, protease and lipase, and spore concentration.