• 한국전산구조공학회논문집
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• 제33권6호
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• pp.419-426
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• 2020

본 연구에서는 철골편심가새골조 시스템을 대상으로 다목적최적화기법을 통해 설계를 수행하고 그 결과를 분석하였다. 최적화 설계를 위해 유전 알고리즘의 일종인 NSGA-II를 활용하였다. 여기서, 목적함수는 이율배반적 관계를 갖는 구조물량과 층간변위로 하여 최소화되고, 제약조건에는 구조기준에서 요구하는 내력비, 링크의 회전각 등을 포함하였다. 제약조건은 최적화 알고리즘 내에서 각 항목을 위반할수록 목적함수 값을 크게 증가시키는 벌금함수의 형태를 가지고 있다. 설계기준에서 EBF 시스템의 설계규정은 링크 부재만 항복이 허용되며 나머지 부재는 링크 항복 시 발생되는 부재력을 탄성상태에서 견디도록 의도한 역량설계법에 기초한다. 그러나 최적화를 통해 도출된 결과 중 일부는 구조기준의 설계조항은 만족하지만 특정층 링크에 소성변형이 집중되어 연약층을 형성함으로써 기준에서 의도하는 역량설계의 원칙을 위배하는 결과가 나타났다. 이를 해결하기 위해 모든 링크의 전단 초과강도계수 중 최대값이 최소값의 1.25배를 넘지 않도록 하는 제약식을 추가하였다. 새로운 제약식을 추가한 경우 모든 최적해는 설계기준과 역량설계의 원칙을 준수하는 것으로 나타났다. 모든 설계안에서 보 경간에 대한 링크의 길이비는 전단링크의 범주에 해당하는 10% ~ 14%였다. 전체적으로 설계안들은 링크의 초과강도 계수비가 가장 지배적인 제약으로 작용하였으며, 구조기준의 요구사항 중 층간변위와 내력비 등의 항목에서 허용치에 비해 매우 보수적으로 설계되었다.

• Journal of the Optical Society of Korea
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• 제19권1호
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• pp.55-62
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• 2015

In this study we demonstrate ultrahigh-resolution spectral domain optical coherence tomography (UHR SD-OCT) with a linear-wavenumber (k) spectrometer, to accelerate signal processing and to display two-dimensional (2-D) images in real time. First, we performed a numerical simulation to find the optimal parameters for the linear-k spectrometer to achieve ultrahigh axial resolution, such as the number of grooves in a grating, the material for a dispersive prism, and the rotational angle between the grating and the dispersive prism. We found that a grating with 1200 grooves and an F2 equilateral prism at a rotational angle of $26.07^{\circ}$, in combination with a lens of focal length 85.1 mm, are suitable for UHR SD-OCT with the imaging depth range (limited by spectrometer resolution) set at 2.0 mm. As guided by the simulation results, we constructed the linear-k spectrometer needed to implement a UHR SD-OCT. The actual imaging depth range was measured to be approximately 2.1 mm, and axial resolution of $3.8{\mu}m$ in air was achieved, corresponding to $2.8{\mu}m$ in tissue (n = 1.35). The sensitivity was -91 dB with -10 dB roll-off at 1.5 mm depth. We demonstrated a 128.2 fps acquisition rate for OCT images with 800 lines/frame, by taking advantage of NVIDIA's compute unified device architecture (CUDA) technology, which allowed for real-time signal processing compatible with the speed of the spectrometer's data acquisition.

• 대한전자공학회논문지TC
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• 제37권3호
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• pp.9-17
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• 2000

본 논문에서는 Quadrature Detector를 이용하여 4FSK 신호 변복조 시스템에 대한 전반적인 분석 및 시뮬레이터를 구현하였다. 구현 기준은 무선 호출시스템 표준인 FLEX 규격을 따랐으며 이에 따라 Pre-modulation 필터 및 데이터 프레임을 구성하였다. 심볼동기 알고리즘은 128bit 구간동안의 프리앰블 패턴을 이용하여 심볼동기를 획득할 수 있는 효율적인 개루프 방식을 제안하였으며, 다양한 UW 검출 방식 중 최적 UW 검출방식인 비주기자기상관 우수코드에 의한 32bit의 최적 UW 패턴을 제안하였다. 아울러 Quadrature Detector의 BER 특성을 AWGN 환경에서 뿐만 아니라 페이딩환경에서 BCH Coding과 Interleaving을 적용해 부호이득을 분석하였다

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1085-1091
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• 2009

A phytase with high activity at low temperatures has great potential for feed applications, especially in aquaculture. Therefore, this study used a degenerate PCR and TAIL PCR to clone a phytase gene from Erwinia carotovora var. carotovota, the cause of soft rot of vegetables in the ground or during cold storage. The full-length 2.5-kb fragment included an open reading frame of 1,302 bp and encoded a putative phytase of 45.3 kDa with a 50% amino acid identity to the Klebsiella pneumoniae phytase. The phytase contained the active site RHGXRXP and HD sequence motifs that are typical of histidine acid phosphatases. The enzyme was expressed in Escherichia coli, purified, and displayed the following characteristics: a high catalytic activity at low temperatures (retaining over 24% activity at $5^{\circ}C$) and remarkably thermal lability (losing >96% activity after incubation at $60^{\circ}C$ for 2 min). The optimal phytase activity occurred at pH 5.5 and ${\sim}49^{\circ}C$, and the enzyme activity rapidly decreased above $40^{\circ}C$. When compared with mesophilic counterparts, the phytase not only exhibited a high activity at a low temperature, but also had a low $K_m$ and high $k_{cat}$. These temperature characteristics and kinetic parameters are consistent with low-temperature-active enzymes. To our knowledge, this would appear to be the first report of a low-temperature-active phytase and its heterogeneous expression.

• Journal of Microbiology and Biotechnology
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• 제22권3호
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• pp.390-399
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• 2012

Endoglucanase gene egIV was cloned from Trichoderma viride AS 3.3711, an important cellulose-producing fungus, by using an RT-PCR protocol. The egIV cDNA is 1,297 bp in length and contains a 1,035 bp open reading frame encoding a 344 amino acid protein with an estimated molecular mass of 35.5 kDa and isoelectronic point (pI) of 5.29. The expression of gene egIV in T. viride AS 3.3711 could be induced by sucrose, corn straw, carboxymethylcellulose (CMC), or microcrystalline cellulose, but especially by CMC. The transcripts of egIV were regulated under these substrates, but the expression level of the egIV gene could be inhibited by glucose and fructose. Three recombinant vectors, pYES2-xegIV, $pYES2M{\alpha}$-egIV, and $pYES2M{\alpha}$-xegIV, were constructed to express the egIV gene in Saccharomyces cerevisiae H158. The CMCase activity of yeast transformants $IpYES2M{\alpha}$-xegIV was higher than that of transformant IpYES2-xegIV or $IpYES2M{\alpha}$-egIV, with the highest activity of 0.13 U/ml at induction for 48 h, illustrating that the modified egIV gene could enhance CMCase activity and that $MF{\alpha}$ signal peptide from S. cerevisiae could regulate exogenous gene expression more effectively in S. cerevisiae. The recombinant EGIV enzyme was stable at pH 3.5 to 7.5 and temperature of $35^{\circ}C$ to $65^{\circ}C$. The optimal reaction condition for EGIV enzyme activity was at the temperature of $55^{\circ}C$, pH of 5.0, 0.75 mM $Ba^{2+}$, and using CMC as substrate. Under these conditions, the highest activity of EGIV enzyme in transformant $IpYES2M{\alpha}$-xegIV was 0.18 U/ml. These properties would provide technical parameters for utilizing cellulose in industrial bioethanol production.