• Title/Summary/Keyword: Optic microscope

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A Understanding of the Temporal Stem

  • Choi, Chan-Young;Han, Seong-Rok;Yee, Gi-Taek;Lee, Chae-Heuck
    • Journal of Korean Neurosurgical Society
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    • v.47 no.5
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    • pp.365-369
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    • 2010
  • Objective : There has been inconsistency about definition of the temporal stem despite of several descriptions demonstrating its microanatomy using fiber dissection and/or diffusion tensor tractography. This study was designed to clarify three dimensional configurations of the temporal stem. Methods : The fronto-temporal regions of several formalin-fixed human cerebral hemispheres were dissected under an operating microscope using the fiber dissection technique. The consecutive coronal cuts of the dissected specimens were made to define the relationships of white matter tracts comprising the temporal stem and the subcortical gray matters (thalamus, caudate nucleus, amygdala) with inferior limiting (circular) sulcus of insula. Results : The inferior limiting sulcus of insula, limen insulae, medial sylvian groove, and caudate nucleus/amygdala were more appropriate anatomical structures than the roof/dorso-lateral wall of the temporal horn and lateral geniculate body which were used to describe previously for delineating the temporal stem. The particular space located inside the line connecting the inferior limiting sulcus of insula, limen insulae, medial sylvian groove/amygdala, and tail of caudate nucleus could be documented. This space included the extreme capsule, uncinate fasciculus, inferior occipito-frontal fasciculus, anterior commissure, ansa peduncularis, and inferior thalamic peduncle including optic radiations, whereas the stria terminalis, cingulum, fimbria, and inferior longitudinal fiber of the temporal lobe were not passing through this space. Also, this continued posteriorly along the caudate nucleus and limiting sulcus of the insula. Conclusion : The temporal stem is white matter fibers passing through a particular space of the temporal lobe located inside the line connecting the inferior limiting sulcus of insula, limen insulae, medial sylvian groove/amygdala, and tail of caudate nucleus. The three dimensional configurations of the temporal stem are expected to give the very useful anatomical and surgical insights in the temporal lobe.

Studies on a Microtissue of Ostericum Root Powder (강활(羌活) 분말(粉末)의 현미조직(顯微組織)에 관한 연구(硏究))

  • Kim, Hyeong-Cheol;Han, Hyo-Sang;Lee, Young-Jong
    • The Korea Journal of Herbology
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    • v.21 no.4
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    • pp.183-187
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    • 2006
  • Objectives : Southern, northern, and wild types of Osterici Radix, and Notopterigii Rhizoma et Radix imported from China are circulated in Korea. Morphological identification was attempted by comparing powder forms of these materials. Methods : To make the specimen, the samples were grinded, and the powder was mounted by a solution of Glycerin, Acetic acid and Water at the rate of 1 : 1 : 1. The specimen was mounted by optic microscope, photographs were taken and the characteristics were sketched in lines. Result : 1. Korean Osterici Radix was yellow-brown, but Chinese Notopterigii Rhizoma et Radix was dark-brown. 2. Oil ducts were mostly broken, and most of secretory cells were narrow and long, including yellow or light yellow secretion and starch grain. 3. Parenchyma cells were rectangular, and southern type of Osterici Radix and Chinese Notopterigii Rhizoma et Radix include lots of starch grain in parenchyma cells, while northern type of Osterici Radix and wild Osterici Radix include a little. 4. Vessels were usually reticulate vessel, and the diameter of the vessels of Korean Osterici Radix was $25{\sim}88{\mu}m$, while that of the vessels of Chinese Notopterigii Rhizoma et Radix was $13{\sim}52{\mu}m$. 5. The surface of accessory cells were light brown with the shape of irregular polygon. Conclusion : Southern type of Osterici Radix, nothern type of Osterici Radix, wild Osterici Radix, and Chinese Notopterigii Rhizoma et Radix were all alike, except the amount of starch grain in parenchyma cells.

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A Study on the Microstructure of Organic Ultra Thin Films and Phase Transition of Langmuir Films in BAM (BAM을 이용한 L막의 상전이 현상과 유기초박막의 미세구조에 관한 연구)

  • Kim, Byung-Geun;Chon, Dong-Kue;Kim, Young-Keun;Lee, Kyung-Sup
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.07b
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    • pp.938-941
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    • 2003
  • It is well known that the state of existence of molecules on the surface of water changes during compression of the molecules. Electric methods, such as measurement of the surface potential or displacement current are also useful for investigating dynamic changes of molecular state on the water surface during compression and Transformation of molecular film occurs only usually in air-water interface, 2 dimensions domain's growth and crash are achieved. Organic thin film that consist of growth of domain can understand correct special quality of accumulation film supplying information about fine structure and properties of matter of device observing information and so on that is surface forward player and optic enemy using AFM one of SPM application by nano electronics. In this paper Langmuir (L) that is one of basis technology to manufacture of organic matter device using biology material PBDG that is kind of polypeptide that have biology adaptedness. The Experiment method used ${\pi}-A$ isotherm and BAM(Brewster Angle Microscopy), using the BAM, we can to the molecular orientation of monolayer on the water surface and directly see the morphology of the films on water subphase as well as that of the films.

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Femto-second Laser Ablation Process for Si Wafer Through-hole (펨토초 레이저 어블레이션을 이용한 Si 웨이퍼의 미세 관통 홀 가공)

  • Kim, Joo-Seok;Sim, Hyung-Sub;Lee, Seong-Hyuk;Shin, Young-Eui
    • Journal of the Microelectronics and Packaging Society
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    • v.14 no.3
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    • pp.29-36
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    • 2007
  • The main objectives of this study are to investigate the micro-scale energy transfer mechanism for silicon wafer and to find an efficient way for fabrication of silicon wafer through-hole by using the femtosecond pulse laser ablation. In addition, the electron-phonon interactions during laser irradiation are discussed and the carrier number density and temperatures are estimated. In particular, the present study observes the shapes of silicon wafer through-hole with $100\;{\mu}m$ diameter and it also measures the heat-affected area and the ablation depths fur different laser fluences by using the optic microscope and the three-dimensional profile measurement technique. First, from numerical investigation, it is found that the nonequilibrium state exists between electrons and phonons during laser irradiation. From experimental results, it should be noted that the heat-affected area increases with laser fluence, and the optimal conditions for through-hole formation with minimum heat affected zone are finally obtained.

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Effects of the root conditioning treatments after Nd:YAG laser irradiation on in vitro human gingival fibroblast attachment to root surfaces (Nd:YAG 레이저조사 후 치근의 처치방법들이 치근면 치은섬유아세포부착에 미치는 영향에 관한 연구)

  • Moon, Hye-Seong;Lim, Kee-Jung;Kim, Byung-Ock;Han, Kyung-Yoon
    • Journal of Periodontal and Implant Science
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    • v.26 no.3
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    • pp.701-713
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    • 1996
  • The purpose of this study was to evaluate the biocompatibility of the Nd:YAG lased root surface followed by root planing and/or tetracyline-HCI(T.C.-HCI) conditioning. $30,4mm{\times}4mm$ root segments were obtained from unerupted third molars and 21, periodontally involved root segments. The treatment groups were as follows : (1) healthy root cementum surface groups : 1) control(non-treated group), 2) lased only, 3) lased/root planed, and 4) lased/T.C.-HCI. (2) diseased root cementum surface groups : 1) control(root planed only), 2) lased/root planed, and 3) lased/root planed/T.C.-HCI. The specimens were treated with a Nd:YAG laser using a $320{\mu}m$ noncontact optic fiber handpiece with an energy setting of 1.5W($114.6J/cm^2$), 2.0W($152.9J/cm^2$), 5.0W($382J/cm^2$) for one minute. The fiber was held perpendicular to the petri dish(NUNC) 2cm apart in an attempt to expose the entire root segments equally. Human gingival fibroblasts were cultured from explants of normal interdental gingival tissue obtained during third morlar extraction. The attachment assay was performed with third-generation fibroblasts. The numbers of gingival fibroblasts attached to the root surface were counted on each specimen under the light microscope, and were statistically analyzed by the oneway ANOVA followed by Tukey's test in SPSS/PC+programs. The results were as follows : 1) In healthy root cementum surfaces, lased/root planed groups exhibited a significantly increased fibroblast attachment compared to controls, lased only, and lased/T.C.-HCI groups(p<0.05), 2) In diseased root cementum surfaces, laser treatment followed by root planing and/or T.C.HCl groups exhibited a increased tendency of fibroblast attachment compared to root planed only group. The results suggest that laser treatment followed by root planing and/or T.C.-HCl would appear necessary so as to render the root surface biocompatible.

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An effect of laser irradiation on periodontal pocket tissue (레이저 조사가 치주낭 조직에 미치는 영향)

  • Han, Kyeong-Yoon;Kim, Sang-Mok;Kim, Byung-Ock;Kim, Hyun-Sub;Lim, Kee-Jung
    • Journal of Periodontal and Implant Science
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    • v.26 no.2
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    • pp.511-521
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    • 1996
  • Periodontal pocket is one of the most frequently developed clinical feature on the teeth with periodontal disease. In order to determine the gingival curettage effect of intrapocket irradiation of a pulsed Nd : YAG laser on periodontally involved teeth, bilateral 60 teeth with $4{\sim}6mm$ in probing pocket depth 1 week after supragingival scaling were selected. On half of them the intrapocket irradiation($300{\mu}m$ fiber optic, 1.5W power, for 2min.) of a pulsed-Nd : YAG laser(EL.EN.EN06O, Italy) was applied as the lased group. On the contralateral 30 teeth the subgingival curettage was accomplished by Gracey curettes as the curattage group. The periodontal pocket tissues were surgically excised by the modified Widman flap technique immediately after the intrapocket irradiation or subgingival curettage, subsequently fixed with 10% neutral formalin, sectioned in $4{\sim}6{\mu}m$ thickness, and stained with hematoxylin-eosin. Surface characteristics and incomplete removal of the pocket epithelium were evaluated under light microscope. And the difference between the lased group and the curettage group was statistically analyzed by Chi-square test in Microstat program. The results were as follows ; 1. The plane surface was observed more frequently in the curettage group(73.3%) than in the lased group(23.3%), and the rough surface was observed more frequently in the lased grOoup(63.3%) than in the curettage group(6.7%)(p<0.05). 2. The rate of incomplete removal of the pocket epithelium was relatively high in both the lased group(76.6%) and the curettage group(86.6%), and there was no significant difference between the lased group and the curettage group(p>0.l). The results suggest that the further studies including various power control of laser should be succeeded in order to obtain more favorable results by the intrapocket irradiation of a pulsed Nd:YAG laser than the subgingival curettage with Gracey curettes.

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A STUDY ON THE OPTIMAL ILLUMINATION POWER OF DIFOTI (DIFOTI 영상 최적화를 위한 광량에 대한 연구)

  • Kim, Jong-Bin;Kim, Jong-Soo;Yoo, Seung-Hoon;Kim, Yong-Kee
    • Journal of the korean academy of Pediatric Dentistry
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    • v.37 no.1
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    • pp.13-23
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    • 2010
  • This study was performed to compare the quality of image processing between the newly developed prototype using light emitting diode(LED) and the conventional $DIFOTI^{TM}$ system(EOS Inc., USA). To estimate the optimal light emitting power for the improved images, primary enamel surfaces treated under Carbopol 907 de-mineralizing solution were taken daily during 20 days of experimental periods by both DIFOTI systems. The results of comparative analyses on the images obtained from both systems with polarized image as gold standard can be summarized as follows: 1. Trans-illumination indices of images taken from primary enamel surfaces were decreased with time in both systems. 2. The differences of intensity of luminance between sound and de-mineralized enamel surface in prototype DIFOTI system was shown to be relatively smaller than conventional $DIFOTI^{TM}$ system. 3. From the comparative analysis of images from both DIFOTI system with polarized images as gold standard, the difference between sound and de-mineralized enamel surface of intensity of luminance of $DIFOTI^{TM}$ system was more correlated to polarized images than prototype of DIFOTI system. With the optimal LED emitting power, the control of aperture of digital camera is considered as the another key factor to improve the DIFOTI images. For the best image quality and analysis, the development of the improved image processing software is required.

Effect of a Pulsed Nd:YAG laser irradiation on human gingival tissues (파동형 Nd:YAG 레이저조사가 인체 치은조직에 미치는 영향)

  • Kang, Kyung-Dong;Kim, Chun-Suk;Kim, Hyung-Soo;Kim, Hyun-Seop;Kim, Byung-Ock;Han, Kyung-Yoon
    • Journal of Periodontal and Implant Science
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    • v.26 no.4
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    • pp.989-1002
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    • 1996
  • The purpose of this study was to determine the effect of a pulsed Nd:YAG laser irradiation on human gingival tissues. The patients, who were planned to be treated by clinical crown lengthening procedure and gingivectomy, were selected. All the patients received oral hygiene instruction, scaling and root planing at preoperation. The crest of gingival tissue on upper and lower anterior teeth was irradiated by a pulsed Nd:YAG laser(El. EN. EN060, Italy) with a fiber optic of 300 m in contact mode for 20 seconds. Gingival tissues were divided into 4 groups according to the laser power of 1.0W(10Hz, 100mJ), 2.0W(20Hz, 100mJ), 3.0W(30Hz, 100mJ) and 4.0W(40Hz, 100mJ). Immediately after the laser irradiation, the specimens were excised, fixed 10% neutral formalin, sectioned $4-6{\mu}m$ thick, stained by Hematoxylin-Eosin and Periodic Acid Schiff stain and observed under light microscope. The removed tissue depth and the coagulated layer depth due to a laser irradiation by a laser irradiation were measured on the microphotographs. The difference of measurements according to the different laser power was statistical1y analyzed by Kruskal Wallis Test with SAS program. The results were as follows : 1. In histologic findings of irradiated gingival tissues; a. In the irradiated gingival specimen with 1.0W laser power, some vesicles were observed in limited superficial layer of gingival epithelium. b. In the irradiated gingival specimen with 2.0W and 3.0W laser power, the epithelium was almost removed except for the traces of viable basal cell remnants at ret peg, and coagulation necrosis related with the thermal effect of laser was noted. c. In the irradiated gingival specimen with 4.0W laser power, complete removal of epithelium, partial removal of underlying connective tissue, and the coagulation necrosis of subjacent gingival tissue were shown. 2. The removed tissue depth was deeper in the irradiated specimens with higher power. There was a statistical significance in the difference of removed tissue depth between 1.0W group ($44.54{\pm}6.99um$) and 3.0W group ($99.75{\pm}6.64{\mu}m$), and between 1.0W group($44.54{\pm}6.99{\mu}m$) and 4.0W group($111.36{\pm}4.50{\mu}m$), and between 2.0W group($98.01{\pm}4.53{\mu}m$) and 4.0W group($111.36{\pm}4.50{\mu}m$)(P<0.05). 3. The coagulated layer depth was deeper in the irradiated specimens with higher power. There was a statistical significance in the difference of coagulated layer depth between 1.0W group($31.82{\pm}8.99{\mu}m$) and 3.0W group($55.99{\pm}20.94{\mu}m$), and between 1.0W group($31.82{\pm}8.99{\mu}m$) and 4.0W group($83.68{\pm}10.34{\mu}m$)(P<0.05). From this study, the results demonstrated that the effects of a pulsed Nd:YAG laser irradiation on gingival tissues seemed to depend on the laser power and that the irradiation with high power could be harmful to adjacent healthy tissue.

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