• Membrane Journal
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• v.20 no.2
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• pp.120-126
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• 2010

In this study, the effect of surface deposition of $TiO_2$ nanoparticles at polyethersulfone (PES) microfiltraiton (MF) membrane on humic acid fouling was investigated. The effect was observed as a function of crystal structures of $TiO_2$ nanoparticles and solution chemistries including pH and divalent cation such as calcium. Our results showed clearly that $TiO_2$-deposited membrane could mitigate membrane fouling significantly. However, this effect was observed to be dependent upon crystal structures of $TiO_2$ nanoparticles and solution chemistries. In the absence of calcium, fouling mitigation was less pronounced for both anatase and hybrid $TiO_2$-deposited membrane than for rutile $TiO_2$-deposited membrane while opposite trend was observed after addition of calcium. In the presence of calcium, the adsorption of humic acid to $TiO_2$-deposited membrane can be reduced by electrostatic repulsions between humic acid and $TiO_2$ surface. Addition of calcium provided further beneficial effect on fouling mitigation particularly at higher pH for the anatase $TiO_2$ deposited membrane, implying that both increased hydrophilicity due to $TiO_2$ nanoparticles and negative surface charge of the membrane should affect fouling mitigation. However, rutile $TiO_2$ having more inertness generally than the anatase $TiO_2$ showed relatively robust effect on the fouling mitigation regardless of solution properties.

• Korean Journal of Plant Tissue Culture
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• v.25 no.6
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• pp.453-475
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• 1998

During the 100 years since the initial discovery of meiotic phenomenon many brilliant aspects have been elucidated, but further researches based on light microscopy alone as an experimental tool have been found to have some limits and shortcomings. By the use of electron microscopy and armed with the advanced knowledges on modern genetics and biochemistry it has been possible to applu molecular technology in gaining information on the detailed aspects of meiosis. As synapsis takes place, a three-layered proteinous structure called the synatonemal complex starts to form in the space between the homologous chromosomes. To be more precise, it begins to form along the paired chromosomes early in the prophase I of meiotic division. The mechanism that leads to precise point-by-point pairing between homologous chromocomes division. The mechamism that leads to precise point-by-point pairing between homologous chromosomes remains to be ascertained. Several items of information, however, suggest that chromsome alignment leading to synapsis may be mediated somehow by the nuclear membrane. Pachytene bivalents in eukaryotes are firmly attached to the inner niclear membrane at both termini. This attached begins with unpaired leptotene chromosomes that already have developed a lateral element. Once attached, the loptotene chromosomes begin to synapse. A number of different models have been proposed to account for genetic recombination via exchange between DNA strands following their breakage and subsequent reunion in new arrangement. One of the models accounting for molecular recombination leading to chromatid exchange and chiasma formation was first proposed in 1964 by Holliday, and 30 years later still a modified version of his model is favored. Nicks are made by endomuclease at corresponding sites on one strant of each DNA duplex in nonsister chromatid of a bivalent during prophase 1 of meiosis. The nicked strands loop-out and two strands reassociate into an exchanged arrangement, which is sealed by ligase. The remaining intact strand of each duplex is nicked at a site opposite the cross-over, and the exposed ends are digested by exonuclease action. Considerable progress has been made in recent years in the effort to define the molecular and organization features of the centromere region in the yeast chromosome. Centromere core region of the DNA duplex is flanked by 15 densely packed nucleosomes on ons side and by 3 packed nucleosomes on the other side, that is, 2000 bp on one side and 400 400 bp in the other side. All the telomeres of a given species share a common DNA sequence. Two ends of each chromosome are virtually identical. At the end of each chromosome there exist two kinds of DNA sequence" simple telpmeric sequences and telpmere-associated sequencies. Various studies of telomere replication, function, and behabior are now in progress, all greatly aided by molecular methods. During nuclear division in mitosis as well as in meiosis, the nucleili disappear by the time of metaphase and reappear during nuclear reorganizations in telophase. When telophase begins, small nucleoli form at the NOR of each nucleolar-organizing chromosome, enlarge, and fuse to form one or more large nucleoli. Nucleolus is a special structure attached top a specific nucleolar-organizing region located at a specific site of a particular chromosome. The nucleolus is a vertical factory for the synthesis of rRNAs and the assenbly of ribosome subunit precursors.sors.

• Pediatric Infection and Vaccine
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• v.21 no.2
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• pp.104-113
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• 2014

Purpose: We compared the clinical manifestations of patients with tsutsugamushi disease between children and adults. Methods: From January 2003 to December 2012, 768 patients diagnosed with tsutsugamushi disease were retrospectively reviewed, and the clinical characteristics, laboratory findings, and complications were compared between children and adults. Results: No patterns of annual increases in the number of patients were noted in both children and adults. The higher incidences occurred in October and November respectively. By gender, male outnumbered female in children, but the opposite trend was seen in adults. By residential area, the urban distribution of children was higher than that of adults. Rashes (P =0.001) and eschar (P =0.004) were more common in children, while myalgia was more common in adults. Children had a high prevalence of anemia (P =0.041), and low incidence rates of thrombocytopenia, abnormal liver and renal function. Children yielded better results in the duration of their hospital stay and the incidence of complications (P <0.001). A comparison of the therapeutic effects of doxycycline and macrolide antibiotics, which was performed only on the children, did not reveal any significant differences. Conclusion: Compared to adults, children had higher incidence rates of male patients and more often suffered from rashes and eschar. Children yielded better results in the laboratory findings and duration of the hospital stay and complications. Therefore, when children are suspected to have tsutsugamushi disease, especially during its peak occurrence period, detailed physical examination and serological test should be performed to ensure a prompt diagnosis, and the use of macrolide antibiotics, which have fewer side effects, is expected to yield the same therapeutic effects.

• Journal of Korean Society of Transportation
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• v.22 no.7 s.78
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• pp.131-137
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• 2004

Conventional traffic signal optimization models assume that green intervals for pedestrian crossings are given as exogenous inputs such as minimum green intervals for straight-ahead movements. As the result, in reality, the green intervals of traffic movements may not distribute adequately by the volume/saturation-flow of them. In this paper, we proposed signal optimization models formulated in BMILP to integrate pedestrian crossings into traffic movements under under-saturated traffic flow. The model simultaneously optimizes traffic and pedestrian movements to minimize weighted queues of primary queues during red interval and secondary queues during queue clearance time. A set of linear objective function and constraints set up to ensure the conditions with respect to pedestrian and traffic maneuvers. Numerical examples are given by pedestrian green intervals and the number of pedestrian crossings located at an arm. Optimization results illustrated that pedestrian green intervals using proposed models are greater than those using TRANSYT-7F, but opposite in the ratios of pedestrian green intervals to the cycle lengths. The simulation results show that proposed models are superior to TRANSYT-7F in reducing delay, where the longer the pedestrian green interval the greater the effect.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.1
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• pp.33-40
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• 2015

Soybean is very useful crop to supply vegetable protein for human. Supply of soybean is increased because it has useful ingredient. Recently, cultivation of soybean in paddy field is increasing due to the increase of rice stockpile in Korea. Hence, in this study, expression of protein was identified regarding different environment for cultivation to investigate the effect of different environment on protein expression. Two-dimensional electrophoresis was performed to investigate the expression of protein using image analysis program to measure degree of protein expression in numerical value. Hannam-kong, Beakcheon-Kong, Hwangkeum-Kong, and Danwon-Kong were used as plant material. 2-DE combined with image analysis revealed that each degree of protein expression of Hannam-Kong and Hwangkeum-Kong in upland field was higher than degree of protein expression in paddy field. However, in case of Beackcheon-Kong, the phenomenon was opposite. In Danwon-kong, the degree of protein expression was not different between up-land field and paddy field. To this end, major protein spots were not different between paddy field and upland field among all cultivars. It could be suggested that protein expression is not severely different by various environment, but different environment affects degree of protein expression.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.5 no.2
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• pp.86-94
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• 2000

In order to extract the spatio-temporal characteristics of long-term variability of the net heat flux on the sea surface in the East Asian marginal seas, empirical orthogonal function (EOF) analysis was conducted using data set calculated every 12 hours interval during 1978-1995. Among the first three modes explaining 73% of the total variance, the first mode having high peak at 1 year period indicates high variability area around the Sandong Peninsula and central and northeastern part of the East Sea. In the second mode which has spatial distribution of dipole type at the north and south, the peaks appear at 3.6 year and 2.3 year cycles. Time coefficient of the second EOF is believed to have close relation with the E1 Nino and has out-of-phase variation with NINO3 SST. Lagged correlation between NINO3 SST and time coefficient of the second EOF indicates four month time delay in the NINO3 SST. In the third mode which has opposite sign at the east and west, the periodicity of 6-9 year cycle has relatively clear appearance compared to other two EOFs. Also, high heat loss exceeding 800 W/$m^{2}$ in winter time occured at the south part of the Sandong Peninsula and Vladivostok. It reveals more frequent occurrence of about two times at the Sandong Peninsula than Vladivostok. The event is concentrated in January at Vladivostok, but it occurs primarily in December and January at the Sandong Peninsula.

• Korean Journal of Environmental Agriculture
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• v.17 no.1
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• pp.76-83
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• 1998

The most remarkable aspect in the hormesis law is that dose of harmful agents can produce effect that are diametrically opposite to the effect found with high doses of the same agent. Minute quantities of a harmful agent bring about very small change in the organism and control mechanisms appear to subjugate normal processes to place the organism in a state of albert and repair. The stimulated organism in more responsive to changes in environmental factors than it did before being alerted. Routine functions, including repair and defense, have priority for available energy and matetial. The alerted organism utilizes nutrients more efficiently, grows faster, shows improved defense, and lives longer. Accelerated germination, sprouting, growth, development, blooming and ripening, and increased crop yield and resistance to disease are found in plants. Another concept supported by the data in that low doses of ionizing radiation provide increased resistance to subsequent high doses of radiation. The hormesis varies with subject plant, variety, state of seed, environmental and cultural conditions, physiologic function measured, dose rate and total exposure. The results of hormesis are less consistently found, probably due to the great number of uncontrolled variables in the experiments. The general dosage for radiation homlesis in about 100 (10 to 1,000) times ambient or 100 (10 to 1,000) times less than a definitely harmful dose, but these must be modified to the occasion. Although little is known about most mechanisms of homzesis reaction, overcompensation of repair mechanism is offered as one mechanism.

• Atmosphere
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• v.16 no.2
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• pp.97-110
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• 2006

This study investigates the response of a typhoon model to the change of the sea surface temperature (SST) throughout the model integration. The SST change is parameterized as a formulae of which the magnitude is given as a function of not only the intensity and the size but the moving speed of tropical cyclone. The formulae is constructed by referring to many previous observational and numerical studies on the SST cooling with the passage of tropical cyclones. Since the parameterized cooling formulae is based on the mathematical expression, the resemblance between the prescribed SST cooling and the observed one during the period of the numerical experiment is not complete nor satisfactory. The agreements between the prescribed and the observed SST even over the swath of the typhoon passage differ from case to case. Numerical experiments are undertaken with and without prescribing the SST cooling. The results with the SST cooling do not show clear evidence in improving the track prediction compared to those of the without-experiments. SST cooling in the model shows its swath along the incomplete simulated track so that the magnitude and the distribution of the sea surface cooling does not resemble completely with the observed one. However, we have observed a little improvement in the intensity prediction in terms of the central pressure of the tropical cyclone in some cases. In case where the model without the SST treatment is not able to yield a correct prediction of the filling of the tropical cyclone especially in the decaying stage, the pulling effect given by the SST cooling alleviates the over-deepening of the model so that the central pressure approaches toward the observed value. However, the opposite case when the SST treatment makes the prediction worse may also be possible. In general when the sea surface temperature is reduced, the amount of the sensible and the latent heat from the ocean surface become also reduced, which results in the weakening of the storms comparing to the constant SST case. It turns out to be the case also in our experiments. The weakening is realized in the central pressure, maximum wind, horizontal temperature gradient, etc.

• Journal of Dental Rehabilitation and Applied Science
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• v.16 no.2
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• pp.113-122
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• 2000

The reinforced composte resin as the esthetic operative material continuously has been studied because the porcelain fused metal prosthesis is widely used for its excellent esthetics, rigidity and marginal integrity, but it has low fracture resistance against the tensile strength and stress, attrition of the opposite teeth. The reinforced composite resin is well adapt with the dental alloy but it is low the shear bond strength with the dental alloy vs the porcelain fused metal prosthesis, and then has been studied continuously. The purpose of the study was to examine how metal was the higher shear bond strength among the dental alloy was used to the reinforced composite resin and to find the effect that the particle size of sandblasting influenced the shear bond strength. We built up the reinforced composite resin with 4 mm in diameter, 3 mm in height on circular alloy with 5 mm in diameter, 2 mm in height. Type II gold, type IV gold, and Ag-Pd alloy was used as alloys and $50{\mu}m$, $110{\mu}m$, $250{\mu}m$ of the particle size was sandblasted at each alloy in bonding between alloy and resin. We made 90 secimens of 10 per each group and we measured the shear bond strength using the Instron($M100EC^{(R)}$, Mecmesin Co., England). The obtained results were as follows : 1. In comparison among each alloys, Ag-Pd alloy had the highest shear bond strength and the shear bond strength was decreased significantly in the sequence of the type II gold and type IV gold(P<0.001). 2. In comparison according to the size of sandblasting particle, (1) In Ag-Pd alloy, shear bond strength was decreased in the sequence of $110{\mu}m$, $250{\mu}m$, $50{\mu}m$ and there were significant difference in all the group. (P<0.05) (2) In type II gold, it was decreased in the sequence of $250{\mu}m$, $50{\mu}m$, $110{\mu}m$ and there were significant difference. (P<0.05) (3) In type IV gold, it was decreased in the sequence of $110{\mu}m$, $50{\mu}m$, $250{\mu}m$. There were significant difference between the group of $110{\mu}m$ and $50{\mu}m$, the group of $110{\mu}m$ and 250, but there were no significant difference in the group of $50{\mu}m$ and $250{\mu}m$. 3. The highest shear bond strength according to the size of sandblasting particle was $110{\mu}m$ in Ag-Pd alloy and type IV gold, $250{\mu}m$ in type II gold.

• Animal Bioscience
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• v.34 no.6
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• pp.957-966
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• 2021

Objective: Ovarian follicular development, which dependent on the proliferation and differentiation of granulosa cells (GCs), is a complex biological process in which miRNA plays an important role. Our previous study showed that miR-458b-5p is associated with ovarian follicular development in chicken. The detailed function and molecular mechanism of miR-458b-5p in GCs is unclear. Methods: The luciferase reporter assay was used to verify the targeting relationship between miR-458b-5p and catenin beta-1 (CTNNB1), which is an important transcriptional regulatory factor of the Wnt/β-catenin pathway. The cell counting kit-8 (CCK-8) assay, flow cytometry with propidium iodide (PI) and annexin V-fluorescein isothiocyanate (FITC) labeling were applied to explore the effect of miR-458b-5p on proliferation, cell cycle and apoptosis of chicken GCs. Quantitative real-time polymerase chain reaction and Western blot were used to detect the mRNA and protein expression levels. Results: We demonstrated that the expression of miR-458b-5p and CTNNB1 showed the opposite relationship in GCs and theca cells of hierarchical follicles. The luciferase reporter assay confirmed that CTNNB1 is the direct target of miR-458b-5p. Using CCK-8 assay and flow cytometry with PI and Annexin V-FITC labeling, we observed that transfection with the miR-458b-5p mimics significantly reduced proliferation and has no effects on apoptosis of chicken GCs. In addition, miR-458b-5p decreased the mRNA and protein expression of CD44 molecule and matrix metallopeptidase 7, which are the downstream effectors of CTNNB1 in Wnt/β-Catenin pathway and play functional roles in cell proliferation. Conclusion: Taken together, the data indicate that miR-458b-5p regulates ovarian GCs proliferation through Wnt/β-catenin signaling pathway by targeting CTNNB1, suggesting that miR-458b-5p and its target gene CTNNB1 may potentially play a role in chicken ovarian follicular development.