• Title/Summary/Keyword: Operonfusion, oxygen-rehulated genetic loci

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A study of aerobic and anaerobic inducible genes using Mu dJ(Km lac) operon fusion in salmonella typhimurium (Salmonella typhimurium에서 Mu dJ(Km lac) operon fusion을 이용한 산소, 무산소 유도 유전자에 관한 연구)

  • 김종선;우영대;박종희;김영권;이인수;박용근
    • Korean Journal of Microbiology
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    • v.27 no.3
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    • pp.201-209
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    • 1989
  • Using Mu dJ(Km lac) operon fusion, several oxygen-regulated genetic loci were identified in Salmonella typhimurium. Nine aerobically inducible(oxi) and thirteen anaerobically inducible(ani) operon fusions were newly identified. Based on the control by oxrA regulatory locus, the ani-lacZ fusions were grouped into two classes: class I loci were regulated by oxrA regulatory locus; class II genes were not affected by this locus. Some of the ani-lacZ fusions had required growth in CAA and LB before they exhibited the inducible phinotype. Most of all ani-lacZ fusions were repressed by nitrate and fumarate. Three of the ani loci were mapped into $59{\pm}0.14$ map unit (YK114), $64{\pm}0.5$ map unit(YK120), and $93{\pm}0.29$ map unit(YK112) by testing the cotransduction frequency, respectively.

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