• The Korean Journal of Zoology
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• v.30 no.1
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• pp.89-98
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• 1987

The research of fused oocytes was conducted to investigate the in vitro mejotic maturation of immature oocytes (GV oocytes) fused with oocytes in germinal vesicle breakdown (GVBD oocytes) in the presence of dbcAMP which is known as one of the strong inhibitors to GVBD. The immature oocytes fused together as well as those fused with GVBD oocytes proceeded to GVBD in 3 hr culture in plain medium. But in the medium containing dbcAMP (100$\mu$g/ml), the immature oocytes fused together did not show any GVBD and thus the fusion itself could not affect the inhibitory activity of dbcAMP. However, all of the immature oocytes fused with GVBD oocytes underwent GVBD in 3 hr culture despite of the presence of dbcAMP. When the culture was extended to 20 hr, nearly all of the immature oocytes fused together were still arrested at the GV stage in the presence of dbcAMP. But most of the fused oocytes which had shown GVBD during 3 hr culture developed to metaphase II stage extruding one or two polar bodies regardless of the presence of dbcAMP. In this experiment, it was found that two sets of the metaphase chromosomes were somewhat concomitant with a pair of the polar bodies in the fused egg. Upon the results of the present studies, it is assumed that there may be a maturation promoting factor(s) in the cytoplasm of the GVBD occytes, and this factor(s) possibly nullifies the function of dbcAMP.

• Korean Journal of Animal Reproduction
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• v.24 no.4
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• pp.407-417
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• 2000

The present study was to examine effects of various electrical stimulus treatments used for electro-fusion on the preimplantation development of bovine nuclear transfer (NT) embryos with fetal fibroblast cells. Fetal fibroblast cells were isolated from one fetus at day 45 of gestation in Holstein cow, and passaged 3 to 4 times before being transferred into enucleated oocytes. Single fibroblast cells were individually placed into the perivitelline space of enucleated oocytes by using a micromanipulator. At first, the fusion and developmental rates of reconstructed oocytes were compared between different electric stimulation conditions. When fusion of the reconstructed oocyte was induced by different electric pulse periods (15, 30 and 45 $\mu$sec) at a DC pulse of 1.8 kV/cm, 15 (45.5%, 120/264) or 30 $\mu$ sec group (43.9%, 106/241) showed a higher fusion rate than 45 $\mu$sec group (23.2%, 58/250, P＜0.05). However, no difference was detected in the development rate of the fused oocytes to blastocysts between groups. Next experiment was to examine the effects of different electrical field strengths (1.5, 1.8 and 2.1 kV/cm) for 15 $\mu$sec at electrofusion on in vitro development of the NT embryos. As results, there was no difference in the fusion and developmental rates of the NT embryos between electrical strength (P＞0.05). Finally, developmental competence of bovine NT embryos with somatic cells was compared with IVF-derived embryos. Of enucleated oocytes fused with fibroblast cells, 27.4% (75/274) developed to the blastocyst stage, which is similar to that (24.5%, 58/237) of IVF-derived embryos. However, mean nuclei number of NT blastocysts was smaller than that of IVF-derived blastocysts. Thus, we have established an optimal condition (1.8 kV/cm, 15 $\mu$sec) for electric fusion of bovine NT oocytes with somatic cells. The present study indicates that bovine reconstructed embryos with somatic cells normally develop to blastocyst stage in vitro, although having smaller nuclei numbers of blastocysts as compared to IVF-derived embryos.

• Journal of the Korea Society of Computer and Information
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• v.19 no.6
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• pp.19-26
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• 2014

An ensemble classifier system is a widely-used multi-classifier system, which combines the results from each classifier and, as a result, achieves better classification result than any single classifier used. Several methods have been used to build an ensemble classifier including boosting, which is a cascade method where misclassified examples in previous stage are used to boost the performance in current stage. Boosting is, however, a serial method which does not form a complete feedback loop. In this paper, proposed is context sensitive SVM ensemble (CASE) which adopts SVM, one of the best classifiers in term of classification rate, as a basic classifier and clustering method to divide feature space into contexts. As CASE divides feature space and trains SVMs simultaneously, the result from one component can be applied to the other and CASE achieves better result than boosting. Experimental results prove the usefulness of the proposed method.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2003.05a
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• pp.787-790
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• 2003

A 4-wavelength optical transceiver system is designed and implemented by using 4 OADMs (optical add-drop multiplexers), WDMs, and optical transceivers. In this new system, the wavelengths of 1510 nm and 1530 nm are used for upload and download signals, respectively, as well as the wavelengths of 1550 nm and 1310 nm which have been utilized in a 2-wavelength optical transceiver systems. The 4-wavelength optical module shows very encouraging pass characteristics of about - 5 dB and isolation characteristics of less than -40 dB, which is configured with two OADMs, 4 couplers, and WDM couplers by fusion splicing. Noise figure (NF) of the one-stage balanced amplifier designed and fabricated for receiver module is 0.38 dB and the amplifying gain is 14.2 dB. S$_{11}$, S$_{22}$ and input, output VSWR are -28.81 dB, -32.08 dB, 1.05 : 1, 1.08 : 1, respectively.y.

• Journal of the Korean Vacuum Society
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• v.16 no.6
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• pp.489-495
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• 2007

The assembly of the main system of the KSTAR tokamak has been recently completed, and the preparation for the 1st plasma and test operations is progressed. The fueling system established for these purposes uses only one port placed at the opposite side of the pumping duct, and has a difficulty of attaining a uniform and fast supply of fuel particles to the plasma. At the base operation stage after finishing the test operation, the fueling system must be improved to provide a uniform fueling and a feed-back control in accordance with a high-density tokamak plasma maintained for a long period. As a part for understanding the points to be improved in the fueling system, a Monte Carlo simulation on the gas fueling into the tokamak plasma has been executed. After modeling the vacuum vessel and the plasma of quasi-D shapes as tori of rectangular cross-sections, the influences of the position and the number of the fueling inputs on the particle density distribution for a given pumping probability and mean free path were investigated.

• IMMUNE NETWORK
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• v.2 no.4
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• pp.233-241
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• 2002

Background: Monoclonal antibodies (mAb) of rodent origin are produced with ease by hybridoma fusion technique, and have been successfully used as therapeutic reagents for humans after humanization by genetic engineering. However, utilization of these antibodies for therapeutic purpose has been limited by the fact that they act as immunogens in human body causing undesired side effects. So far, there have been several attempts to produce human mAbs for effective in vivo diagnostic or therapeutic reagents including the use of humanized xenomouse that is generated by mating knockout mice which lost Ig heavy and light chain genes by homologous recombination and transgenic mice having both human Ig heavy and light gene loci in their genome. Methods: Genomic DNA fragments of mouse Ig heavy and light chain were obtained from a mouse brain ${\lambda}$ genomic library by PCR screening and cloned into a targeting vector with ultimate goal of generating Ig knockout mouse. Results: Through PCR screening of the genomic library, three heavy chain and three light chain Ig gene fragments were identified, and restriction map of one of the heavy chain gene fragments was determined. Then heavy chain Ig gene fragments were subcloned into a targeting vector. The resulting construct was introduced into embryonic stem cells. Antibiotic selection of transfected cells is under the progress. Conclusion: Generation of xenomouse is particularly important in medical biotechnology. However, this goal is not easily achieved due to the technical difficulties as well as huge financial expenses. Although we are in the early stage of a long-term project, our results, at least, partially contribute the successful generation of humanized xenomouse in Korea.

• Korean Journal of Veterinary Research
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• v.33 no.1
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• pp.151-169
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• 1993

The present study was carried out to investigate the best condition for nuclear-cytoplasm fusion and in vitro culture of nuclear transplanted embryos and to investigate the production of nuclear transplanted offsprings. The nuclei from 2-, 4- and 8-cell mouse embryos were transferred into enucleated 2-cell embryos, and the reconstituted embryos were submitted to direct current(DC) pulses at output voltage of 1.0, 1.5 and 2.0 kV/cm for 100, 150 and $200{\mu}$ sec to induce cell fusion. 1. The culture of intact or zona cut 2-cell embryos in the medium supplemented with cytochalasin B($5{\mu}g/m{\ell}$) and colcemide($0.1{\mu}g/m{\ell}$)for 30 and 60 minutes did not affect the development to later stage. 2. The in vitro developmental rates of group A(a nucleus from one of the blastomeres was removed) and B(electrofusion of group A) were significantly lower than that of control group(p<0.01). 3. When nuclear transplanted embryos were submitted to electrofusion, the significantly higher fusion rates of 2-cell donor nuclei were achieved at the electric field strength of DC 1.5kV/cm for 100 and $150{\mu}$ sec, DC 2.0 kV/cm for $100{\sim}200{\mu}$ sec than DC 1.0 kV/cm for 100 and $150{\mu}$ sec(p<0.01). The significantly higher fusion rates of 4-cell donor nuclei were achieved at DC 2.0 kV/cm for 100 and $150{\mu}$ sec than DC 1.0kV/cm for $100{\sim}200{\mu}$ sec(p<0.01). These fusion rates in 8-cell donor nuclei were 88.7~99.3%. 4. The developmental potency to blastocyst in 2- and 4-cell donor nuclei was significantly higher in DC 1.0 and 2.0 kV/cm for $100{\sim}200{\mu}$ sec treated group and DC 2.0 kV/cm for 150 and $200{\mu}$ sec treated group (p<0.01). The developmental potency to blastocyst in 8-cell donor nuclei was significantly higher in DC 2.0 kV/cm for $100{\mu}$ sec treated group than in DC 1.0 kV/cm for $100{\mu}$ sec treated group and DC 2.0 kV/cm for 150 and $200{\mu}$ sec treated group(p<001). 5. The developmental potency to blastocyst after nuclear transplantation was significantly higher in 2-cell donor nuclei than in 8-cell donor nuclei(p<0.01). 6. The success rate of nuclear injection into enucleated 2-cell embryos was significantly higher in 2-cell donor nuclei than in 4- or 8-cell donor nuclei(p<0.01). 7. The culture time taken for the nuclear transplanted 2-cell embryos to blastocyst stage was significantly longer in 2-cell donor nuclei than in 8-cell donor nuclei(p<0.01). 8. There was no significant difference in the developmental potency of nuclear transplanted embryos within the concentration of EGF at 0 to 15 ng per $m{\ell}$ of BMOC-3 solution. 9. The production rates of offspring after transfer of nuclear transplanted embryos to recipient mouse were significantly higher in 2-cell donor nuclei than in 8-cell donor nuclei(p<0.01).

• Journal of Broadcast Engineering
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• v.23 no.3
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• pp.421-430
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• 2018

Many voice recognition systems use methods such as MFCC, HMM to acknowledge human voice. This recognition method is designed to analyze only a targeted sound which normally appears between a human and a device one. However, the recognition capability is limited when there is a group sound formed with diversity in wider frequency range such as dog barking and indoor sounds. The frequency of overlapped sound resides in a wide range, up to 20KHz, which is higher than a voice. This paper proposes the new recognition method which provides wider frequency range by conjugating the Wideband Sound Spectrogram and the Keras Sequential Model based on DNN. The wideband sound spectrogram is adopted to analyze and verify diverse sounds from wide frequency range as it is designed to extract features and also classify as explained. The KSM is employed for the pattern recognition using extracted features from the WSS to improve sound recognition quality. The experiment verified that the proposed WSS and KSM excellently classified the targeted sound among noisy environment; overlapped sounds such as dog barking and indoor sounds. Furthermore, the paper shows a stage by stage analyzation and comparison of the factors' influences on the recognition and its characteristics according to various levels of noise.

• Journal of the Korean Society for Precision Engineering
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• v.21 no.12
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• pp.100-108
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• 2004

In reverse engineering, data acquisition methodology can generally be categorized into contacting and non-contacting types. Recently, researches on hybrid or sensor fusion of the two types have been increasing. In addition, efficient construction of a geometric model from the measurement data is required, where considerable amount of user interaction to classify and localize regions of interest is inevitable. Our research focuses on the classification of each bounded region into a pre-defined feature shape fer a hybrid measuring scheme, where the overall procedures are described as fellows. Firstly, the physical model is digitized by a non-contacting laser scanner which rapidly provides cloud-of-points data. Secondly, the overall digitized data are approximated to a z-map model. Each bounding curve of a region of interest (featured area) can be 1.aced out based on our previous research. Then each confined area is systematically classified into one of the pre-defined feature types such as floor, wall, strip or volume, followed by a more accurate measuring step via a contacting probe. Assigned to each feature is a specific digitizing path topology which may reflect its own geometric character. The research can play an important role in minimizing user interaction at the stage of digitizing path planning.

• Journal of Institute of Control, Robotics and Systems
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• v.18 no.8
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• pp.780-786
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• 2012

Surveillance has become one of promising application areas of wireless sensor networks which allow for pervasive monitoring of concerned environmental phenomena by facilitating context awareness through sensor fusion. Existing systems that depend on a postmortem context analysis of sensor data on a centralized server expose several shortcomings, including a single point of failure, wasteful energy consumption due to unnecessary data transfer as well as deficiency of scalability. As an opposite direction, this paper proposes an energy-efficient distributed context-aware surveillance in which sensor nodes in the wireless sensor network collaborate with neighbors in a distributed manner to analyze and aware surrounding context. We design and implement multi-modal sensor stations for use as sensor nodes in our wireless sensor network implementing our distributed context awareness. This paper presents an initial experimental performance result of our proposed system. Results show that multi-modal sensor performance of our sensor station, a key enabling factor for distributed context awareness, is comparable to each independent sensor setting. They also show that its initial performance of context-awareness is satisfactory for a set of introductory surveillance scenarios in the current interim stage of our ongoing research.