• Journal of the Korean Chemical Society
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• v.61 no.4
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• pp.191-196
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• 2017

One-phase method to prevent the initial formation of Ag(I) thiolate layered materal from the mixture of $AgNO_3$ and thiols was previously developed to generate TP (Thiolate-Protected)-nanosilver. In this modified method, $AgNO_3$ is added to the mixtures of $NaBH_4$ and thiols in ethanol. This method was so successful that it was applied to synthesize TP-nanogold and nanoplatinum. The synthesis and characterization of these nanoparticles by ultraviolet-visible (UV-vis) absorption spectra, transmission electron microscopy (TEM) pictures, X-ray powder differaction (XRD) patterns and infrared(IR) spectra are described. The results show that colloidal nanoparticles are spherical or oval shape and the mean sizes for TP-nanogold and nanoplatinum are about 3~7 nm and below 2 nm, respectively. The conformation of polymethylene [$-(CH_2)_7-$] sequence in octanethiolate attached to nanogold was elucidated as trans.

• Journal of Science of Art and Design
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• v.1
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• pp.63-93
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• 1999

This treatise begins with finding a meaning of Paul Klee's . It is pretty simpleto choose the square of Klee. The most important formative language for twenty century is abstraction. The element of speaking for abstraction issquare. The artists are trying to contain the nature and universe in the square. The role of magic square consisting with small squares of Klee is crystallized. The other side, the test of this study is a method analysis. The method analysis is changing while concept and style have been changing according to a period. The existing method analysis is an iconology used many times in Art history. This treatise introduces France symbolists, Bernard Cocula and Claude Peyroutet's analysis of a meaning of image(Semantique do l'image) who were applied to Modern Art. based on Iconography. It also applies to analysis of artwork of Klee. Cocula's is developed from one phase to five phase step by step. The first phase deals with an appearance of artwork. Subsequently, the second phase is directly adjacent to personal feeling and impression. This is an adequate method for image study in the analysis of modern arts. This phase makes it a rule to enjoy talking with artworks above all. The third phase begins with this question 'What do you see? (que voyons-nous?).' The applies exhaustively and strictly to complicated image artworks which need an elaborate analysis. It is very hard but audiences must try to maintain neutrality in front of artwork because cord formation and interpretation should be formed objectively. The meaning analysis and interpretation of the forth phase begins with this question 'what is the image rouse'(qu'evoque cote image?).' This phase is the most important in a process of symbolic analysis. The audience investigates personal elements and common elements. The fifth is synthetic analysis and interpretation phase. The synthesis is last phase and it reaches a valuation and a conclusion. Namely, the synthesis phase makes up synthesis conclusion, summarizes image character, and completes value adjudication. Sometimes it completes no conclusions in a silence. This study found a new possible analysis example from Paul Klee's work. The study emphasizes square analysis and interpretation and uses . The analysis of artwork by Cocula's is an example of the most important work of Klee's three artworks. The first analysis of artwork is and the second one is . The third one is . In these analyses, Klee usedmagic square 'to make natural pictorial element and to explain organic living things.'

• Korean Journal of Veterinary Service
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• v.28 no.3
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• pp.285-294
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• 2005

This study was conducted to investigate the extraction method for the determination of organochlorine, organophosphorus and synthetic pyrethroid pesticide residues in beef fat by gas chromatography-mass selective detector(GC-MSD). Twenty one pesticide residues in fat were determined using a simple and rapid procedure based on solid- phase extraction(SPE) clean-up cartridges with octadecyl $(C_{18})-bonded$ porous silica, florisil, $10\%$ deactivated florisil. a tandem $C_{18}$ and florisil and a tandem $C_{18}$ and $10\%$ deactivated florisil. Solvent-solvent extraction using acetonitrile was not satisfied to eliminate fat interference for pesticide residue analysis by GC-MSD, and the recoveries of the method in fat ranged from 16.2 to $57.3\%$ except DDT$(83.2\%)$. The recoveries of SPE methods using a tandem $C_{18}$ and Florisil was $59.6\~123.8\%$ except fenitrothion $(135.2\%)$. the SPE method was verified the satisfactory performance of pre-treatment for pesticide residues analysis in fat by GC-MSD. The efficiency of florisil deactivated with $10\%$ water has been not proved significantly on recoveries of pesticide residues in fat.

• Speech Sciences
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• v.1
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• pp.295-314
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• 1997

In this study, we will implement a flexible formant analysis and synthesis system. In the analysis part, the two-channel (i.e., speech & EGG signals) approach is investigated for accurate estimation of formant information. The EGG signal is used for extracting exact pitch information that is needed for the pitch synchronous LPC analysis and closed phase LPC analysis. In the synthesis part, Klatt formant synthesizer is modified so that the user can change synthesis parameters arbitarily. Experimental results demonstrate the superiority of the two-channel analysis method over the one-channel(speech signal only) method in analysis as well as in synthesis. The implemented system is expected to be very helpful for studing the effects of synthesis parameters on the quality of synthetic speech and for the development of Korean text-to-speech(TTS) system with the formant synthesis method.

• Korean Journal of Materials Research
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• v.14 no.5
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• pp.358-362
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• 2004

$Si_3$$N_4$ ceramics have been identified as one of the promising structural ceramics. This study has been carried out to investigate of the synthetic behaviors of $Si_3$$N_4$ derived from domestic silica-stone by direct nitriding method. The silicon nitridation reaction has been studied in the temperature range of $1300～1550^{\circ}C$. Below the $1400^{\circ}C$, the nitriding rate was measured to be 16%. For the temperatures higher than the $1400^{\circ}C$, $\beta$-$Si_3$$N_4$ phase was formed mainly, and the nitriding rate showed above 98%. With the increasing of sample weight of silicon powder, the nitriding rate and $\beta$-$Si_3$$N_4$ phase increased at $1400^{\circ}C$ for 2 hours. The shape and particle size of$ Si_3$$N_4$ powder synthesized at $1400^{\circ}C$ for 2 hours showed the irregular angular-type and 10 $\mu\textrm{m}$, respectively.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.8
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• pp.97-103
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• 2014

Signal processing technique of linear frequency modulation-frequency shift keying (LFM-FSK) waveform has been introduced for rear view radars of an automobile. LFM-FSK waveform consists of two sequential stepped frequency waveforms with some frequency offset, and thus, can be used to generate inverse synthetic aperture radar (ISAR) images of rear view target of an automobile. However, ISAR images can often be blurred due to inter-pulse phase errors. To resolve this problem, one-dimensional (1-D) entropies of high resolution range profiles (HRRP) are minimized with the help of particle swarm optimization (PSO). The searching space used in PSO is adaptively adjusted by the use of information on the target's velocity obtained from LFM-FSK waveforms. Simulation results show that the proposed method can generate well-focused ISAR images.

• Korean Journal of Remote Sensing
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• v.31 no.2
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• pp.101-110
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• 2015

Synthetic Aperture Radar Interferometry (InSAR) is affected by various noise source such as atmospheric artifact, orbital error, processing noise etc.. Especially, one of the dominant noise source for long-wave SAR system, such as ALOS PALSAR (L-band SAR satellite) is the ionosphere effect because phase delays on radar pulse through the ionosphere are proportional to the radar wavelength. To avoid misinterpret of phase signal in the interferogram, it is necessary to detect and correct ionospheric errors. Recently, a MAI (Multipler Aperture SAR Interferometry) based ionospheric correction method has been proposed and considered one of the effective method to reduce phase errors by ionospheric effect. In this paper, we introduce the MAI-based method for ionospheric correction. Moreover we propose an efficient method that apply the method over non-coherent area using directional filter. Finally, we apply the proposed method to the ALOS PALSAR pairs, which include the west sea coast region in Korea. A polynomial fitting method, which is frequently adopted in InSAR processing, has been applied for the mitigation of phase distortion by the orbital error. However, the interferogram still has low frequency of Sin pattern along the azimuth direction. In contrast, after we applied the proposed method for ionospheric correction, the low frequency pattern is mitigated and the profile results has stable phase variation values within ${\pm}1rad$. Our results show that this method provides a promising way to correct orbital and ionospheric artifact and would be important technique to improve the accuracy and the availability for L-band or P-band systems.

• Journal of Institute of Control, Robotics and Systems
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• v.22 no.2
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• pp.139-146
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• 2016

Recently, popularity of 3D technology has been growing significantly and it has many application parts in the various fields of industry. In order to overcome the limitations of 2D machine vision technologies based on 2D image, we need the 3D measurement technologies. There are many 3D measurement methods as such scanning probe microscope, phase shifting interferometry, confocal scanning microscope, white-light scanning interferometry, and so on. In this paper, we have used the extended depth of focus (EDF) algorithm among 3D measurement methods. The EDF algorithm is the method which extracts the 3D information from 2D images acquired by short range depth camera. In this paper, we propose the EDF algorithm using the edge informations of images and the average values of all pixel on z-axis to improve the performance of conventional method. To verify the performance of the proposed method, we use the various synthetic images made by point spread function(PSF) algorithm. We can correctly make a comparison between the performance of proposed method and conventional one because the depth information of these synthetic images was known. Through the experimental results, the PSNR of the proposed algorithm was improved about 1 ~ 30 dB than conventional method.

• Geophysics and Geophysical Exploration
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• v.14 no.3
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• pp.234-241
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• 2011

Recently, exploration and development of oil sands are thriving due to high oil price. Because oil sands reservoir usually exists as a thin layer, multicomponent VSP, which has the advantage of the high-resolution around the borehole, is more effective than surface seismic survey in exploring oil sand reservoir. In addition, prestack phase-screen migration is effective for multicomponent seismic data because it is based on an one-way wave equation. In this study, we examined the applicability of the prestack phase-screen migration for multicomponent RVSP data to image the thin oil sand reservoir. As a preprocessing tool, we presented a method for separating P-wave and PS-wave from multicomponent RVSP data by using incidence angle and rotation matrix. To verify it, we have applied the developed wavefield separation method to synthetic data obtained from the velocity model including a horizontal layer and dipping layers. Also, we compared the migrated image by using P-wave with that by using PS-wave. As a result, the PS-wave migrated image has higher resolution and wide coverage than P-wave migrated image. Finally, we have applied the prestack phase-screen migration to the synthetic data from the velocity model simulating oil sand reservoir in Canada. The results show that the PS-wave migrated image describe the top and bottom boundaries of the thin oil sand reservoir more clearly than the P-wave migrated image.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.408-420
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• 2014

Yeast surface-displayed antibody libraries provide an efficient and quantitative screening resource for given antigens, but suffer from typically modest library sizes owing to low yeast transformation efficiency. Yeast mating is an attractive method for overcoming the limit of yeast transformation to construct a large, combinatorial antibody library, but the optimal conditions have not been reported. Here, we report a large synthetic human Fab (antigen binding fragment) yeast surface-displayed library generated by stepwise optimization of yeast mating conditions. We first constructed HC (heavy chain) and LC (light chain) libraries, where all of the six CDRs (complementarity-determining regions) of the variable domains were diversified mimicking the human germline antibody repertoires by degenerate codons, onto single frameworks of VH3-23 and $V{\kappa}1$-16 germline sequences, in two haploid cells of opposite mating types. Yeast mating conditions were optimized in the order of cell density, media pH, and cell growth phase, yielding a mating efficiency of ~58% between the two haploid cells carrying HC and LC libraries. We constructed two combinatorial Fab libraries with CDR-H3 of 9 or 11 residues in length with colony diversities of more than $10^9$ by one round of yeast mating between the two haploid HC and LC libraries, with modest diversity sizes of ${\sim}10^7$. The synthetic human Fab yeast-displayed libraries exhibited relative amino acid compositions in each position of the six CDRs that were very similar to those of the designed repertoires, suggesting that they are a promising source for human Fab antibody screening.