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Comparison of Nitrogenous Extractives, Amino Acids in Wild and Cultured Bastard (천연 및 양식산 넙치의 함질소엑스분과 아미노산조성)

  • Oh, Kwang-Soo;Lee, Hyeung-Joo;Sung, Dae-Whan;Lee, Eung-Ho
    • Korean Journal of Food Science and Technology
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    • v.20 no.6
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    • pp.873-877
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    • 1988
  • Nitrogenous extractives and amino acids were analyzed with the dorsal muscle from wild and cultured bastard(Paralichthys olivaceus), and flounder (Pleuronichthys cornutus). Cultured bastard was higher in moisture content, and lower in crude lipid and protein content than those of wild one. Contents of free amino acids from wild and cultured bastard, flounder were 305.03mg/100g, 253.42mg/100g, and 340.10mg/100g, respectively. The profile of free amino acids in these samples was very similar, although there were a relatively difference in glycine, threonine, proline contents. Content of IMP in wild bastard (408.31mg/100g) and cultured one (356.26mg/100g) were higher than that of flounder (178.61mg/100g). Wild bastard contained higher trimeth-ylamine oxide and total creatinine than cultured one. Total amino acid contents of dorsal muscle from wild and cultured bastard, and flounder were 21.94g/100g, 20.12g/100g, and 17.63g/100g, respectively. The major amino acids of these samples were glutamic acid, aspartic acid, leucine and isoleucine.

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Systems for Production of Calves from Hanwoo IVM/IVF/IVC Blastocyst. IV. Direct Transfer of Vitrified and One-Step Diluted Hanwoo Blastocysts

  • 김은영;박세필;김덕임;이문걸;이종우;이금실;박세영;박은미;윤지연
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.73-73
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    • 2001
  • This study was to examine whether the vitrified, one-step diluted and direct transferred Hanwoo IVM/IVF/IVC blastocysts can be successfully survived in vivo and they were succeeded into the live birth. For vitrification, blastocysts were serially exposed in glycerol (G) or/and ethylene glycol (EG) mixtures [10% (v/v) G for 5 min, 10% G plus 20% EG (v/v) for 5 min, and 25% G plus 25% EG (v/v) for 30 sect] which is diluted in 10% FBS added D-PBS. Thawing of straw was carried out in air for 10 sec and then in water bath of $25^{\circ}C$ for 20 sec. One-step dilution within the straw was done in water bath of $25^{\circ}C$ for 1 min. Vitrified and one-step diluted embryos were directly transferred into 36 (natural or hormone induced synchronized) recipient cows in 6 areas of Kyungsang Buk-Do. Pregnancies were confirmed at first when recipient cows did not return to the subsequent estrus cycle, and later by manual palpation per rectum on day 45, 90 and then living calves were derived into parturition. Overall pregnancy was 33.3%(12/36), However, higher pregnancy was obtained when the recipients exhibited estrus one day earlier than the age of transferred embryos (53.3 vs 25.0-27.3%), irrespective of synchronization methods. Also, parous recipients became pregnant higher than nulliparous heifers, And, there were not different in pregnancy rates by the aspect of corpus luteum (CL) quality of recipients (good, 29.4; fair, 37.5; poor, 33.3%). One hundred eight of frozen-thawed Hanwoo blastocysts were directly transferred into 36 recipient cows. In 12 of pregnant cows, 3 cows were aborted and 9 cows were calved [single, 66.7% (6/9): twin, 33.3% (3/9)]. Total embryo implantation rate was 11.1% (12/108). However, 9 Hanwoo calves were lived. Therefore, these results demonstrate that direct transfer technique of vitrified and one-step diluted bovine blastocysts can be applied easily and effectively with the higher pregnancy rate on field trial without the equipment and embryological skills.

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THE DOMINATION COVER PEBBLING NUMBER OF SOME GRAPHS

  • Kim, Ju Young;Kim, Sung Sook
    • Journal of the Chungcheong Mathematical Society
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    • v.19 no.4
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    • pp.403-408
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    • 2006
  • A pebbling move on a connected graph G is taking two pebbles off of one vertex and placing one of them on an adjacent vertex. The domination cover pebbling number ${\psi}(G)$ is the minimum number of pebbles required so that any initial configuration of pebbles can be transformed by a sequence of pebbling moves so that the set of vertices that contain pebbles forms a domination set of G. We determine the domination cover pebbling number for fans, fuses, and pseudo-star.

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The Virtual Waiting Time of the M/G/1 Queue with Customers of n Types of Impatience

  • Bae Jongho
    • Proceedings of the Korean Statistical Society Conference
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    • 2004.11a
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    • pp.289-294
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    • 2004
  • We consider M/G/1 queue in which the customers are classified into n+1 classes by their impatience time. First, we analyze the model of two types of customers; one is the customer with constant impatience duration k and the other is patient customer. The expected busy period of the server and the limiting distribution of the virtual waiting time process are obtained. Then, the model is generalized to the one in which there are classes of customers according to their impatience duration.

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THE EQUITABLE TOTAL CHROMATIC NUMBER OF THE GRAPH $HM(W_n)$

  • Wang, Haiying;Wei, Jianxin
    • Journal of applied mathematics & informatics
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    • v.24 no.1_2
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    • pp.313-323
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    • 2007
  • The equitable total chromatic number ${\chi}_{et}(G)$ of a graph G is the smallest integer ${\kappa}$ for which G has a total ${\kappa}$-coloring such that the number of vertices and edges in any two color classes differ by at most one. In this paper, we determine the equitable total chromatic number of one class of the graphs.

Bioconcentration of IBP, Methidathion and Piperophos in Brachydanio rerio(zebrafish) (Brachydanio rerio(zebrafish)를 이용한 IBP, methidathion 및 piperophos의 생물농축성)

  • 하영득;민경진;이승곤
    • Journal of Environmental Health Sciences
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    • v.27 no.2
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    • pp.108-118
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    • 2001
  • This study was performed to investigate the bioconcentration of IBP, methidathion and piperophos. The BCFs(bioconcentration factor), depuration rate constants for three pesticides in zebrafish(Brachydanio rerio) were measured by OECD guideline 305. The concentration of test pesicides were one-hundredth and one-thousandth concentration of 96-hrs L $C_{50}$ in accordance with OECD guideline 305. The results obtained are summarized as follows: The average BCF values of IBP were 5.31(n=4) and 7.30(n=4) at one-hundredth and one-thousandth concentration of 96-hrs L $C_{50}$ . The average BCF values of methidathion were 8.72(n=4) and 11.25(n=4), the average BCF values of piperophos were 34.30(n=4) and 42.60(n=4). Depuration rate constants of IBP were 0.09( $h^{-1}$ ) and 0.08( $h^{-1}$ ), half-life of IBP were 7.70 and 8.66 at each tested concentration. The concentrations of IBP in zebrafish at low and high concentrations rapidly decreased after 12(0.243$\mu\textrm{g}$/g) and 12 hours(0.040$\mu\textrm{g}$/g). Depuration rate constants of methidathion were 0.40( $h^{-1}$ ), half-life of methidathion were 1.73 at one-hunderdth and of 96-hrs L $C_{50}$ , repectively. The concentrations of methidathion in zebrafish at high concentrations rapidly decreased after 6 hours(0.18 $\mu\textrm{g}$/g). Depuration rate constant of low concentration was no measured because methidathion in zebrafish was depurated in 6 hours. Depuration rate constants of piperophos sere 0.15( $h^{-1}$ ) and 0.44( $h^{-1}$ ), half-life of piperophos were 4.62 and 1.58 at each tested concentration. The concentrations of piperophos in zebrafish at los and high concentrations rapidly decreased after 12(0.26$\mu\textrm{g}$/g) and 6 hours(0.015 $\mu\textrm{g}$/g). It was suggested that high BCF of piperophos was due to high Kow(octanol-water partition coefficient). The possibility of bioconcenration was not likely to be high because of its $K_{DEP}$(depuration rate constant) in the evniroment. It was suggested that low BCF of methidathion showed lowest Kow as well as the most rapid $K_{DEP}$. Therefore, the possibility of bioconcentration was not occured in the enviroment. It was suggested that the BCF dtermined for IBP was lower than that of other pesticides due to high Sw(water solubility), show $K_{DEP}$. Therefore, IBP revealed little bioconcentration effect on in aquatic ecosystem.ystem.

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Hepatoprotective Effects of Chinese Traditional Prescription, Dalwoneum

  • Jun, Jung-Yang;Baek, Hum-Young;Kim, Mi-Hee;Jeong, Ok-Sam;Kim, Youn-Chul
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.272.3-273
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    • 2003
  • Dalwoneum is the one of Chinese traditional prescription used for the treatment of liver disease. This prescription consists of Arecae Semen (12 g), Magnoliae Cortex (9 g), Amomi tsao-ko Fructus (3 g), Anemarrhenae Rhizoma (12 g), Paeoniae Radix (9 g), Scutellariae Radix (12 g), and Glycyrrhizae Radix (3 g). Water extract of Dalwoneum showed the significant hepatoprotective effect on tacrine-induced cytotoxicity in Hep G2 cells. (omitted)

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Identification of the Chicken Meat Allergens (닭고기 중 알레르기 유발성분의 동정)

  • 조은득;김동섭;정기화
    • Biomolecules & Therapeutics
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    • v.9 no.1
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    • pp.7-14
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    • 2001
  • The chicken meat has been reported as one of the food causing allergic reactions predominantly to Korean. At present, several in vitro tests for immunoglobulinG (IgG)-mediated as well as IgE-mediated food allergy are available. 13 clinically chicken meat-allergic patients were investigated together with 4control subjects for identification of chicken meat-specific reactivity by ELISA. Also, protein profile and IgE, IgGtotal and IgG4-reacting allergens were detected by means of sodium dodecyl sulfate-polyacrylamide gel electro-phoresis (SDS-PAGE)and immunoblotting. Chicken meat extracts were prepared as raw, heated, heat and simulated gastric fluid (SGF) treated samples to characterize the stability of allergen to physicochemical treatment. SDS-PAGE revealed 9~200 kDa bands. And in immunoblotting 7 sera were identified most major bands between 10 and 78 kDa. In case of IgE, six proteins (17, 26, 35, 40, 78 kDa) were predominant in heat-treated extract, and the one (35 kDa) was present in SGF-treated preparations. In case of IgG$_{total}$ and IgG4, most of them showed a patters simmilar to IgE. There were significant differences (P<0.05) in IgE, IgG$_{total}$ , IgG4 Abs to chicken meat between the allergic and control subjects in ELISA. In addition, the concentration of IgG4Abs in the challenge-positive subjects was significantly higher than that of control subjects. It is considered that the specific IgE response to chicken meat was rarely prevalent to Koreans. However, the specific IgG4 response play an important role in the development of allergic symptoms.

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