• Title/Summary/Keyword: OmpA

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A User Detection Technique Based on Parallel Orthogonal Matching Pursuit for Large-Scale Random Access Networks (대규모 랜덤 액세스 네트워크에서 병렬 직교매칭퍼슛 기술을 이용한 사용자 검출 기법)

  • Park, Jeonghong;Jung, Bang Chul;Kim, Jinwoo;Kim, Jeong-Pil
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.19 no.6
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    • pp.1313-1320
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    • 2015
  • In this paper, we propose a user detection technique based on parallel orthogonal matching pursuit (POMP) for uplink multi-user random access networks (RANs) with a number of users and receiver antennas. In general RANs, it is difficult to estimate the number of users simultaneously transmitting packets at the receiver because users with data send the data without grant of BS. In this paper, therefore, we modify the original POMP for the RAN and evaluate its performances through extensive computer simulations. Simulation results show that the proposed POMP can effectively detect activated users more than about 2%~8% compared with the conventional OMP in RANs.

Occurrence of canine brucellosis in large kennels and characterization of Brucella canis isolates by PCR-RFLP (집단 개사육농장에서의 Canine Brucellosis 발생 및 PCR-RFLP를 이용한 분리주의 특성조사)

  • Kim, Jong-Wan;Lee, Young-Ju;Tak, Ryun-Bin
    • Korean Journal of Veterinary Research
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    • v.43 no.1
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    • pp.67-75
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    • 2003
  • A total of 260 dogs were randomly selected from two different treed kennels that brucellosis has occurred (group 1, 126 dogs), and random selected breed kennel (group 2, 134 dogs), and monitored for Brucella canis (B. canis) by 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and bacterial culture method. For the differentiation, PCR-RFLP using omp-31, wbkA and per genes used for 52 of B canis strains (strain I) isolated in this study and 3 of B. canis strains (strain II) isolated in 1994 in Korea. 2ME-RSAT revealed that 63/126 dogs (50.0%) and 12/134 dogs (9.0%) were positive in group I and group II, respectively. Bacterial culture revealed that 47/126 dogs (37.3%) and 5/134 dogs (3.7%) were positive in group I and group II, respectively. As the results of PCR-RFLP, $\underline{omp}-31$ was amplified from all Brucella spp, except B. abortus. All B. canis isolates showed unique PCR-RFLP pattern following digestion with Bmel8I. However, all Brucella spp. showed the same PCR-RFLP pattern following digestion with SalI. PCR-RFLP analysis of wbkA revealed that all Brucella spp. showed the same pattern following digestion with HindIII. PCR-RFLP analysis of per revealed that B. abortus 544 and B. melitensis 63/9 showed the same pattern, but different from B. suis and B. canis following digestion with HindIII.

Increased Immunogenicity and Protective Efficacy of a P. aeruginosa Vaccine in Mice Using an Alum and De-O-Acylated Lipooligosaccharide Adjuvant System

  • Ryu, Ji In;Wui, Seo Ri;Ko, Ara;Do, Hien Thi Thu;Lee, Yeon Jeong;Kim, Hark Jun;Rhee, Inmoo;Park, Shin Ae;Kim, Kwang Sung;Cho, Yang Je;Lee, Na Gyong
    • Journal of Microbiology and Biotechnology
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    • v.27 no.8
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    • pp.1539-1548
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    • 2017
  • Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen that commonly causes fatal infections in cystic fibrosis and burn patients as well as in patients who are hospitalized or have impaired immune systems. P. aeruginosa infections are difficult to treat owing to the high resistance of the pathogen to conventional antibiotics. Despite several efforts, no effective prophylactic vaccines against P. aeruginosa are currently available. In this study, we investigated the activity of the CIA06 adjuvant system, which is composed of alum and de-O-acylated lipooligosaccharide, on a P. aeruginosa outer membrane protein (OMP) antigen vaccine in mice. The results indicated that CIA06 significantly increased the antigen-specific IgG titers and opsonophagocytic activity of immune sera against P. aeruginosa. In addition, the antibodies induced by the CIA06-adjuvanted vaccine exhibited higher cross-reactivity with heterologous P. aeruginosa strains. Finally, mice immunized with the CIA06-adjuvanted vaccine were effectively protected from lethal P. aeruginosa challenge. Based on these data, we suggest that the CIA06 adjuvant system might be used to promote the immunogenicity and protective efficacy of the P. aeruginosa OMP vaccine.

Millimeter-Wave(W-Band) Forward-Looking Super-Resolution Radar Imaging via Reweighted ℓ1-Minimization (재가중치 ℓ1-최소화를 통한 밀리미터파(W밴드) 전방 관측 초해상도 레이다 영상 기법)

  • Lee, Hyukjung;Chun, Joohwan;Song, Sungchan
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.28 no.8
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    • pp.636-645
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    • 2017
  • A scanning radar is exploited widely such as for ground surveillance, disaster rescue, and etc. However, the range resolution is limited by transmitted bandwidth and cross-range resolution is limited by beam width. In this paper, we propose a method for super-resolution radar imaging. If the distribution of reflectivity is sparse, the distribution is called sparse signal. That is, the problem could be formulated as compressive sensing problem. In this paper, 2D super-resolution radar image is generated via reweighted ${\ell}_1-Minimization$. In the simulation results, we compared the images obtained by the proposed method with those of the conventional Orthogonal Matching Pursuit(OMP) and Synthetic Aperture Radar(SAR).

Periplasmic Expression of a Recombinant Antibody (MabB9) in Escherichia coli

  • Chang, Hae-Choon;Kwak, Ju-Won
    • Journal of Microbiology and Biotechnology
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    • v.7 no.5
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    • pp.299-304
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    • 1997
  • Expression in the periplasm of Escherichia coli of cloned heavy and light chain cDNAs for Fab fragment of a murine monoclonal antibody MabB9 (${\gamma}2b$, K), specific for human plasma apolipoprotein B-100 of LDL, was studied. For the purpose, a vector for two-cistronic expression of the heavy chain cDNA, at the 5' terminus, and light chain cDNA, at the 3' terminus, was constructed using the signal sequences, pelB (for heavy chain) and ompA (for light chain) in a pET vector system. The constructed vector was transformed into E. coli BL21(DE3). The expressed heavy chain (25 kDa) and light chain (23 kDa) of the antibody molecule were detected in total cell extracts as well as in the periplasmic extracts of E. coli.

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Sparse Index Multiple Access for Multi-Carrier Systems with Precoding

  • Choi, Jinho
    • Journal of Communications and Networks
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    • v.18 no.3
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    • pp.439-445
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    • 2016
  • In this paper, we consider subcarrier-index modulation (SIM) for precoded orthogonal frequency division multiplexing (OFDM) with a few activated subcarriers per user and its generalization to multi-carrier multiple access systems. The resulting multiple access is called sparse index multiple access (SIMA). SIMA can be considered as a combination of multi-carrier code division multiple access (MC-CDMA) and SIM. Thus, SIMA is able to exploit a path diversity gain by (random) spreading over multiple carriers as MC-CDMA. To detect multiple users' signals, a low-complexity detection method is proposed by exploiting the notion of compressive sensing (CS). The derived low-complexity detection method is based on the orthogonal matching pursuit (OMP) algorithm, which is one of greedy algorithms used to estimate sparse signals in CS. From simulation results, we can observe that SIMA can perform better than MC-CDMA when the ratio of the number of users to the number of multi-carrier is low.

A Spatial-Temporal Three-Dimensional Human Pose Reconstruction Framework

  • Nguyen, Xuan Thanh;Ngo, Thi Duyen;Le, Thanh Ha
    • Journal of Information Processing Systems
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    • v.15 no.2
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    • pp.399-409
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    • 2019
  • Three-dimensional (3D) human pose reconstruction from single-view image is a difficult and challenging topic. Existing approaches mostly process frame-by-frame independently while inter-frames are highly correlated in a sequence. In contrast, we introduce a novel spatial-temporal 3D human pose reconstruction framework that leverages both intra and inter-frame relationships in consecutive 2D pose sequences. Orthogonal matching pursuit (OMP) algorithm, pre-trained pose-angle limits and temporal models have been implemented. Several quantitative comparisons between our proposed framework and recent works have been studied on CMU motion capture dataset and Vietnamese traditional dance sequences. Our framework outperforms others by 10% lower of Euclidean reconstruction error and more robust against Gaussian noise. Additionally, it is also important to mention that our reconstructed 3D pose sequences are more natural and smoother than others.

Antimicrobial Agents That Inhibit the Outer Membrane Assembly Machines of Gram-Negative Bacteria

  • Choi, Umji;Lee, Chang-Ro
    • Journal of Microbiology and Biotechnology
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    • v.29 no.1
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    • pp.1-10
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    • 2019
  • Gram-negative pathogens, such as Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii, pose a serious threat to public health worldwide, due to high rates of antibiotic resistance and the lack of development of novel antimicrobial agents targeting Gram-negative bacteria. The outer membrane (OM) of Gram-negative bacteria is a unique architecture that acts as a potent permeability barrier against toxic molecules, such as antibiotics. The OM is composed of phospholipids, lipopolysaccharide (LPS), outer membrane ${\beta}-barrel$ proteins (OMP), and lipoproteins. These components are synthesized in the cytoplasm or in the inner membrane, and are then selectively transported to the OM by the specific transport machines, including the Lol, BAM, and Lpt pathways. In this review, we summarize recent studies on the assembly systems of OM components and analyze studies for the development of inhibitors that target these systems. These analyses show that OM assembly machines have the potential to be a novel attractive drug target of Gram-negative bacteria.

Construction of Methanol-Sensing Escherichia coli by the Introduction of a Paracoccus denitrificans MxaY-Based Chimeric Two-Component System

  • Ganesh, Irisappan;Vidhya, Selvamani;Eom, Gyeong Tae;Hong, Soon Ho
    • Journal of Microbiology and Biotechnology
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    • v.27 no.6
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    • pp.1106-1111
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    • 2017
  • Escherichia coli was engineered to sense methanol by employing a chimeric two-component system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing the Paracoccus denitrificans MxaY with the E. coli EnvZ. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed maximum transcription of ompC and the fluorescence at 0.01% of methanol, respectively. These results suggested that E. coli was successfully engineered to sense methanol by the introduction of chimeric MxaYZ. By using this strategy, various chimeric TCS-based bacterial biosensors can be constructed and used for the development of biochemical-producing recombinant microorganisms.