• Environmental Analysis Health and Toxicology
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• 제17권3호
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• pp.219-224
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• 2002

This study was carried out to examine the effects of bis (tribytyltin)oxide (TBT), endocrine disrupting compounds (EDCs). on the changes of survival, growth and oxygen consumption rate in the oliver flounder, Paralichthys olivaceus. Oliver Flounders were exposed to sublethal concentration of TBT (0, 1.67, 3.20, 6.30 and 12.50 $\mu\textrm{g}$/L) during 6 weeks. Survival rate was decreased in a concentration and exposure period-dependent way and suddenly the reduction of more than 20% occurred at TBT concentration greater than 3.20 $\mu\textrm{g}$/L. after exposure 6 weeks. Growth rate and feed efficiency significantly decreased at concentration greater than 3.20 $\mu\textrm{g}$/L. Oxygen consumption rate was also decreased in a concentration-dependent way and significantly decreased to 17,48 and 67％ than that of the control at the TBT concentration of 3.20, 6.32 and 12.50 $\mu\textrm{g}$/L, respectively. This study revealed that high TBT concentration ($\geq$3.20 $\mu\textrm{g}$/L) reduced growth and oxygen consumption rates of the juvenile oliver flounder suggesting potential influence on the natural mortality of Paralichthys olivaceus in the coastal areas.

• 한국어병학회지
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• 제19권3호
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• pp.235-241
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• 2006

The effects of various temperature shifts on the kinetics of the humoral antibody response in oliver flounder, Paralichthys olivaceus, immunised with formalin-killed Edwardsiella tarda, were determined by measuring the antibody production in vivo and in vitro. When fish acclimated to a high temperature and immunised at that temperature were transferred to a lower temperature (22℃ to 12℃) at a various times after immunisation, the fish showed a weaker immune response than that achieved when the fish were kept at a high environmental temperature. However, in the converse experiment (12℃ to 22℃), the magnitude of the humoral immune response was recovered independent of the time of the transfer after immunisation at low temperature, even though the peak levels of each transferred group did not reach the level found in the positive control group that was maintained and immunised at a high environmental temperature. Hence, these studies provide some evidence that the potential for antibody production in B cells of oliver flounder immunized at high temperature is not impaired by subsequent exposure to low temperature.

• 한국어병학회지
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• 제15권3호
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• pp.99-104
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• 2002

넙치의 생존, 성장 및 대사에 미치는 납의 만성적 독성을 파악하기 위하여 6주 동안 납 0, 80, 150, 230, 420 ${\mu}g/L$농도에 노출시켜 실험을 실시하였다. 넙치의 생존율은 납 농도 230 ${\mu}g/L$이상에서 유의한 감소를 나타냈다. 넙치의 성장률과 사료효율은 납 농도가 증가할수록 감소를 하여 150 ${\mu}g/L$이상의 농도에서는 대조구에 비하여 유의한 감소를 나타냈다. 넙치의 대사율은 납 농도 80, 150, 230, 420 ${\mu}g/L$에서 대조구보다 각각 21.4, 23.8, 36.8, 44.9 %가 저하했으며, 80 ${\mu}g$/L이상의 농도에서는 유의한 감소를 나타냈다.

• 대한수의학회지
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• 제47권3호
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• pp.299-302
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• 2007

Neoheterobothrium hirame (N. hirame) is a large blood-feed gill-worm infecting the highly prized olive flounder. The present study was reported the occurrence and elimination of Neoheterobothrium infection from young cultured oliver flounder in the western coast of Korea. Flounder (weight 18-25 g, length 10-15 cm) were obtained from a cultured farm at Taean of Korea at May 2006. A lot of flounder died with serious anemia. We isolated N. hirame from the buccal cavity wall of dead flounder. Adult parasites were 5-15 mm in total length, with 4 pairs of clamps on the pedunculated haptor and isthmus region embedded in host tissue, while immature and sub-adults were identified the attachment to the gill filaments, rakers and arches with the clamps. All fish with infection were treated with 8% NaCl-supplemented seawater for 1 and 10 min at $22^{\circ}C$, respectively. The significant reduction in mortality (6.7%) was observed among fish treated with 8% salt water for 10 minutes, when compared with that of non-treatment group (100%).

• 한국양식학회지
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• 제12권2호
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• pp.79-89
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• 1999

The recombinant bovine somatotropin (rBST) was administered to the oliver flounder, Paralichthys olivaceus, to know the effects and optimal administration frequency and dosage of the hormone. The experiment was conducted with three different treatment groups (A, B and C) designated based on the duration and administration frequency of rBST and one control (D) from April 14, 1996 to March 16, 1997. The fish of hormone treated groups grew 7.86 to 10.07% (47.45 to 60.75 g in weight) better than the control at the end of the experiment (P<0.05). No significant differences in their growth were detected among treatment groups. The distinct growth improvement was recognized four weeks after completion of the first four hormone administration. When considering water temperatures measured from the experimental tanks, the effect of rBST on the flounder was greater during the period showing relatively lower temperature. The survival rates were higher in treatment groups than in the control, revealing 98.3% (A), 98.4% (B), 97.7% (C) and 93.1% (D) during the first stage of culture; 92.7% (A), 91.3% (B), 86.7% (C) and 80.0% (D) during the second stage of culture.

• 한국양식학회지
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• 제12권1호
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• pp.71-77
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• 1999

The potential utility of injection media (sucrose, PEG, and liposome) was demonstrated for direct gene transfer into olive flounder (Paralichthys olivaceus) muscles. Based on the use of sucrose (final cone. 20%), PEG 8,000 (final cone. 10%) or liposome (twice us of DNA injected), the present injection strategy significantly improved the level of transgene expression as well as persistent duration of expression. The increased amounts of expression in DNA injection with sucrose, PEG, and liposome were as high as from 2.1 to 4.9-folds of conventional TE-based DNA injection. The best result was obtained from injections of liposome-encapsulated DNA in which the expression was detectable at least 32 days after injection when compared to only 8-16 days from TE-based injections.

• 대한수의학회지
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• 제47권3호
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• pp.303-308
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• 2007

The high mortality (65.5%) of young olive flounder (Paralichthys olivaceus) showing skin ulcers occurred in fish obtained from a commercial cultured farm at Donghae on the eastern coast of Korea at March 2006. The dorsal skin showed ulcer lesions and large amount of mucus. We isolated Ichthyobodo sp. from the gill, and Vibrio anguillarum from the liver and kidney of dead and diseased fish. All of the fish infected with the V anguillarum and Ichthyobodo sp. were treated with 100 ppm formalin for 1 h, and treated with oxolinic acid for 4 h at $22^{\circ}C$, respectively. The formalin treated group was observed the low mortality (2.9%) when compared with high mortality of non-treated (82.9%), and of antibiotic treated group (85.9%) against mixed infections by Vibrio anguillarum and Ichthyobodo sp. organisms. We confirmed complete elimination against mixed infections of Vibrio anguillarum and Ichthyobodo sp. with 100 ppm formalin treatment.

• 임산에너지
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• 제23권2호
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• pp.34-43
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• 2004

목탄, 목초액 및 목초탄(목탄80/목초액20)의 사료첨가제로서의 이용 가능성을 도출하고자 국내 주요 양식어종인 넙치의 생장 및 체 조성에 미치는 영향을 조사하였다. 무첨가 대조구와 비교하여 목탄, 목초액 및 목초탄 모두 생장 개선효과를 보였다. 사료효율에 있어서는 대조구와 목탄, 목초탄 첨가구는 유의적인 차이가 없었으나, 목초액은 유의적으로 높은 수치를 나타내었다. 또한 혈액성상은 대조구보다 첨가구에서 모두 높았으며, 특히 목초탄 첨가구가 대조구보다 유의적으로 높아 건강한 어류를 생산하였다. 폐사율은 대조구가 가장 높았으며, 목초액 첨가구와 목초탄 첨가구가 가장 낮았다. 한편 회복 소요시간은 모든 첨가구가 대조구보다 빨랐으며, 목초탄 첨가구가 가장 빠른 회복율을 나타내었다. 따라서 목초탄 첨가구는 생장뿐만 아니라 넙치의 건강도와 활력에서 우수한 결과를 나타내어 넙치의 생장과 면역력의 개선을 위해서는 목초액과 목탄이 혼합된 목초탄 첨가가 적절할 것으로 사료된다.

• 환경생물
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• 제21권1호
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• pp.36-41
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• 2003

연안지역의 환경오염 및 양식용수의 이용과정에 파생될 수 있는 구리의 오염에 따른 넙치의 생존, 대사율, 사료효율 및 성장율에 미치는 구리의 영향을 검토하였다. 4개의 아치사구리 농도(50, 80, 180, 320 $\mu\textrm{g}$ L$^{-1}$)에서 6주동안 실험하였다. 실험기간 동안 넙치는 대조구에서는 실험종료시 까지 사망개체가 전혀 나타나지 않아 100%의 생존율을 나타내었다. 구리 노출농도 180 $\mu\textrm{g}$ L$^{-1}$에서는 노출 4주부터 감소하기 시작하였으며, 노출기간이 길어짐에 따라 지속적으로 감소하여 노출 6주에는 84.0%까지 감소하였다. 또한 구리 노출농도 320 $\mu\textrm{g}$L$^{-1}$에서 노출 2주부터 감소하여 노출 6주에 82.0%로 높은 두 개의 농도구에서 유의한 감소가 나타났다. 산소 소비량은 노출농도 180, 320 $\mu\textrm{g}$ L$^{-1}$에서 대조구보다 각각 27.0, 42.0%가 저하하여 유의한 감소를 나타냈다. 사료효율은 대조구가 43.6%로서 가장 높은 효율을 나타냈고, 노출농도가 320 $\mu\textrm{g}$ L$^{-1}$에서는 30.0%로서 가장 낮은 성장 상태를 보였으며, 노출농도 80 $\mu\textrm{g}$ L$^{-1}$이상에서는 대조구에 비해 유의한 감소를 나타냈다. 성장율은 대조구에서 평균 16.8%로 나타나 가장 높은 성장을 보였으며, 노출농도 320 $\mu\textrm{g}$ L$^{-1}$에서는 5.4%로서 가장 낮은 성장을 보였다. 성장률의 전체적인 양상은 노출농도가 증가할수록 성장이 떨어지는 결과를 보였으며, 사료효율과 마찬가지로 노출농도 80 $\mu\textrm{g}$ L$^{-1}$ 이상에서 유의한 감소를 나타냈다.

• 한국수산과학회지
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• 제56권5호
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• pp.606-614
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• 2023

This study was conducted to optimize the size of olive flounder Paralichthys olivaceus (OF) as a material of sikhae and to investigate the quality characteristics. The results on the protease activity of OF meat, protein and ash contents of the bone, and yields and hardness of fish bone during fermentation time suggest that the suitable fish weight for sikhae was less than 250 g. The proximate compositions of the OF sikhae fermented under optimum condition (fermentation for 9 days at 15℃), were 73.0% moisture, 12.0% crude protein, 1.3% crude fat and 2.4% ash. The salinity, titration acidity and amino acid nitrogen contents per 100 g sikhae were 1.7 g, 2.46 g, and 311.3 mg, respectively. The lactic acid bacteria concentration in the sikhae were 8.84 log CFU/g, which were higher than those (5.78-6.62 log CFU/g) of 5 kind of commercial flounder sikhae. The functional properties, such as ACE inhibitory activity (69.0%), antioxidative activity (69.3%), α-glucosidase inhibitory activity (22.7%), xanthine oxidase inhibitory activity (88.2%), and nitrite scavenging activity (96.4%) of the sikhae were superior to those of 5 kind of commercial flounder sikhae.