• 한국해양바이오학회지
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• 제1권4호
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• pp.268-274
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• 2006

해양 원유 오염원의 제거 및 항진균 활성을 가진 균주를 분리하기 위해 1% crude oil을 탄소원으로 이용하여 생육하는 수십 종의 균주를 분리하였다. 분리된 균주 중 biosurfactant를 생성하는 균주를 1% tributyrin에서 투명환 생성 유무를 통해 선별되었으며, 이를 Bacillus sp. DYL130으로 명명하였다. Bacillus sp. DYL130의 표면장력은 최저 28 mN/m까지 감소되었다. 이 균주로부터 biosurfactant를 정제하기 위해 배양 상등액을 친화성 크로마토그래피와 TLC를 이용하여 정제된 biosurfactant를 얻었다. TLC 결과 0.78정도의 Rf값을 확인하였다. 정제된 biosurfactant의 CMC 값은 35 mg/l인 것으로 나타났으며, 유화활성과 안정성은 soybean oil을 기질로 사용하였을 경우 최대인 것으로 나타났다. Botrytis cineria에 대한 길항작용도 우수한 것으로 보인다.

• 생명과학회지
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• 제26권9호
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• pp.1049-1055
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• 2016

본 연구에서는 고등어 부산물의 활용을 위해 가수분해된 고등어 부산물을 사용하였고, 분리된 유지를 시료로 하여 정제조건과 그 특성을 분석하였다. 탈검은 6% citric acid 수용액으로 시행하였을 때, 인의 함량이 45 mg/kg에서 5.8 mg/kg으로 감소하여 가장 효과적이었으며, 탈산은 8% sodium hydroxide수용액으로 처리하여 산가와 과산화물가가 각각 18.03 mgKOH/g에서 1.19 mg KOH/g과 30.25 meq/kg에서 3.18 meq/kg으로 감소하였다. 탈색은 activated charcoal을 5%로 사용하였을 때 산가와 과산화물가가 각각 0.17 mgKOH/g과 1.21 meq/kg으로 감소하였다. 색도의 변화는 탈산을 거쳤을 때 0.488 nm에서 0.057 nm로 감소하였는데 이후 탈색과정에서는 큰 변화를 보이지 않았다. 불포화 지방산의 조성 증가를 위해 냉침을 행하여 docosahexaenoic acid (DHA)와 eicosapentaenoic acid (EPA)의 함량의 합이 228.83 mg/g으로 측정되었으며, 오메가-3 지방산 함유 유지의 기준에 적합하게 정제하여 제품으로 활용이 가능함을 나타내었다.

• Archives of Pharmacal Research
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• 제20권4호
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• pp.372-374
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• 1997

In order to develop new anti-inflammatory agents having different action mechanisms compared with nonsteroidal and steroidal anti-inflammatory drugs, the culture broths of various actinomycetes isolated from soil were screened using an in vivo mouse ear edma assay and one strain (Streptomyces sp. MT 2705-4: KCTC 8651 P) was selected. Activity-guided purification led to the isolation of a polyether compound, dianemycin. Topically, dianemycin showed a potent anti-inflammatory activity in mouse ear edema induced by croton-oil or arachidonic acid.$ED_{50}$value of dianemycin was found to be 0.8 mg,/ear compared to 0.4 mg/ear of prednisolone in croton-oil ear edema. However, dianemycin did not show the inhibitory activity in UV-erythema and delayed hypersensitivity reaction. These results indicate that dianemycin is a potential topical anti-inflammatory agent.

• 자원리싸이클링
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• 제22권1호
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• pp.72-79
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• 2013

지구환경 문제 및 건강과 관련하여 친환경 제품 개발에 대한 관심이 날로 증대되고 있으며 사용중 산화 등으로 인하여 발생하는 유해물질이 포함된 동/식물성 유지류 등을 환경친화적 제품으로 재활용하는 것은 매우 중요한 기술이다. 본 연구에서는 동식물폐유지 모노머화 재활용 기술에 대한 특허와 논문을 분석하였다. 분석범위는 1976년 ~ 2012년까지의 미국, 유럽연합, 일본, 한국의 등록/공개된 특허와 SCI 논문으로 제한하였다. 특허와 논문은 키워드를 사용하여 수집하였고, 기술의 정의에 의해 필터링 하였다. 특허와 논문의 동향은 연도, 국가, 기업, 기술에 따라 분석하였다. 이에 전처리 정제, 중합용 모노머화, 중합체 제조 기술 모두 미국이 앞서 있는 것으로 나타났다.

• 공업화학
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• 제22권5호
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• pp.495-500
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• 2011

나프타 분해 공정에서 필수적으로 발생되는 분해연료유(PFO, pyrolyzed fuel oil)에서 나프탈렌을 재결정해내고 남는 PFO 잔유물을 이용하여 $300{\sim}800^{\circ}C$에서 질소 조건에서 탄소구조체를 합성하여 보았다. PFO를 헥산이나 메탄올로 처리 후 얻은 탄소물질 프리커서를 열처리하면 $350^{\circ}C$에서는 수 십 ${\mu}m$ 크기의 flake 상의 탄소체가 만들어졌으나, $400^{\circ}C$ 이상에서는 수 ${\mu}m$로 크기가 줄며 공 모양의 탄소구조체로 변형되었다. BET와 XRD 스펙트럼에 따르면 공모양으로 합성된 메조상 탄소체는 큐빅상으로 미세 기공인 mesopore가 아직 잘 발달되지 많은 부정형 탄소임을 알려주고있다.

• Corrosion Science and Technology
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• 제13권1호
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• pp.28-35
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• 2014

Recently, the fuel oil of diesel engines of marine ships has been increasingly changed to heavy oil of low quality as the oil price is getting higher and higher. Therefore, the spiral gear attached at the motor of the oil purifier which plays an important role to purify the heavy oil is also easy to expose at severe environmental condition due to the purification of the heavy oil in higher temperature. Thus, the material of the spiral gear requires a better mechanical strength, wear and corrosion resistance. In this study, the heat treatment(tempering) with various holding time at temperature of $500^{\circ}C$ was carried out to the alloy of Cu-7Al-2.5Si as centrifugal casting, and the properties of both hardness and corrosion resistance with and without heat treatment were investigated with observation of the microstructure and with electrochemical methods, such as measurement of corrosion potential, cathodic and anodic polarization curves, cyclic voltammogram, and a.c. impedance. in natural seawater solution. The ${\alpha}$, ${\beta}^{\prime}$ and ${\gamma}_2$ phases were observed in the material in spite of no heat treatment due to quenching effect of a spin mold. However, their phases, that is, ${\beta}^{\prime}$ and ${\gamma}_2$ phases decreased gradually with increasing the holding time at a constant temperature of $500^{\circ}C$. The hardness more or less decreased with heat treatment, however its corrosion resistance was improved with the heat treatment. Furthermore, the longer holding time, the better corrosion resistance. In addition, when the holding time was 48hrs, its corrosion current density showed the lowest value. The pattern of corroded surface was nearly similar to that of the pitting corrosion, and this morphology was greatly observed in the case of no heat treatment. It is considered that ${\gamma}_2$ phase at the grain boundary was corroded preferentially as an anode. However, the pattern of general corrosion exhibited increasingly due to decreasing the ${\gamma}_2$ phase with heat treatment. Consequently, it is suggested that the corrosion resistance of Cu-7Al-2.5Si alloy can be improved with the heat treatment as a holding time for 48 hrs at $500^{\circ}C$.

• 한국지하수토양환경학회지:지하수토양환경
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• 제27권spc호
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• pp.99-112
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• 2022

From early 2000, many researchers in the groundwater and soil environment remediation project tried to calculate the pollution level and pollution remediation cost and reflect it in the design. In addition, by identifying the movement characteristics of oil pollutants in the underground environment, many researchers tried to derive design factors necessary for pollution purification. However, although the test should be conducted in an area contaminated with oil, the toxicity and risk are too great for testing by deliberately leaking pollutants that are harmful to the human body. And as oil-contaminated areas are promoted by military units such as returned US military bases, there is a limit to access by the general public. In addition, since the indoor simulation test and the field application test have been carried out separately from each other, it was difficult to compare and review various simulation tests Therefore, in this study, PITT (Partitioning Interwell Tracer Test) and analysis methods were specifically presented through actual tests so that field workers could easily use them with the help of the military base and the Korea Rural Community Corporation Soil Environment Restoration Team. However, in order to directly reflect the distribution tracer test results in the pollution remediation design, it is necessary to reduce the analysis errors by comparing the analysis results of the existing soil pollution survey, physical exploration, and numerical modeling. In addition, it is judged to be cautious in the analysis because errors can easily occur due to various factors such as the type of oil at the polluted site, the hydraulic conductivity of the aquifer, and the skill of the researcher.

• Journal of Microbiology and Biotechnology
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• 제6권3호
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• pp.184-188
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• 1996

The Acinetobacter sp. BE-254 isolated from soil sources produced a bioemulsifier in the medium supplemented with n-hexadecane. This bioemulsifier was purified by the procedures of fractionation (ammonium sulfate and chilled acetone), extraction by hexane, and column chromatography on silica gel 60. The results from various color reactions indicated that the bioemulsifier was a glycolipid. The purified emulsifier was very stable at pHs ranging from 4 to 10 and under heat treatment at $100^{\circ}C$ for 30 min. Emulsification activity was also hardly influenced by pH. The critical micelle concentration (CMC) and surface tension at the point ($\gamma_{cmc}$) of the bioemulsifier were approximately 35 mg/l and 30 mN/m, respectively. The bioemulsifier showed a fairly good emulsification activity and stability in comparison with other commercial emulsifiers in the basic formula composed of emulsifier, oil, and water.

• Journal of Plant Biotechnology
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• 제4권3호
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• pp.95-101
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• 2002

Molecular faming has the potential to provide large amounts of recombinant protein for use in diagnostics and as therapeutics. Various strategies have been developed to enhance the expression level, stability, and native folding of recombinant proteins produced in plants. Few investigations into the subcellular distribution of recombinant proteins within plant cells have been published despite the potential to increase the expression level and impact the purification process. This review article discusses the current strategies used for targeting recombinant proteins to various subcellular locations and the advantages of targeting to seed oil bodies for molecular farming applications. Specifically, the affinity capture of antibodies using recombinant oilbodies is discussed.

• Preventive Nutrition and Food Science
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• 제3권4호
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• pp.374-378
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• 1998

The objectives of this study are to determine the optimum process conditions of Korean beeswax and discuss the parameter of adsorption . Purification conditions, such as time, temperature and the type of adsorbents were investigated using the relationship between the properties of refined products and process conditions and theiptimum bleaching condition of Korean beeswax were determined for the first time. The various bleaching conditions were examined by observing the result of Lovibond color, photometric color, methods provided by the American Oil Chemists '(A.O.C.S).The iptimum bleaching temperature was 8$0^{\circ}C$ and the bleaching agents (5% of the weight of beeswax) which consisted of activated clay and activated carbon and a vacuum system were also necessary.