• Food Science and Biotechnology
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• 제18권5호
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• pp.1224-1229
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• 2009

The main objective of this study was to investigate possible application of citron (Citrus junos Sieb. ex TANAKA) seeds, which are massively produced as by-products during citron tea process, into functional food materials. First of all, citron seeds were germinated and produced citron shoots were examined for their functional properties. When contents of flavonoids in citron seeds and their germinated shoots were compared, naringenin, neohesperitin, and hesperitin were remarkably increased in shoots after germination while naringin and didymin were decreased. Concentrations of limonin and nomilin were decreased by germination otherwise their unidentified derivatives were newly formed. A methanol extract of citron shoot had lower $IC_{50}$ values [0.13 and 0.07 mg/mL for 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and ABTS, respectively] than citron seed extract in radical scavenging activities. Addition of 500 mg/mL of citron shoot extract suppressed fat accumulation in 3T3-L1 adipocytes by 36.9%. Oral administration of olive oil along with citron shoot extract (33 mg/kg body weight) to Sprague Dawley rats effectively inhibited absorption of lipid into a body by decreasing blood triglyceride levels from 105.1 to 74.9 mg/dL 2 hr after olive oil administration. According to these results, citron shoot extract as a rich source of flavonoids can be utilized for functional food ingredients with effective antioxidant and anti-adipogenic properties.

• 한국응용과학기술학회지
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• 제12권1호
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• pp.43-46
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• 1995

The antioxidative effect of ethanol extract of ginger on mackerel pike(Colorabis saira) flesh was investigated by periodically measuring TBA value and perioxide value(POV) during storage. The ethanol extract of ginger was added to minced mackerel pike flesh and the fish oil by concentration(2%, 4%, 6%, 8%). Then the minced flesh was storaged at $-5^{\circ}C$ and the fish oil was incubated at $40^{\circ}C$. The TBA values of minced flesh were approximately increased in inverse proportion to concentration of ginger extract. Peroxide values were attended with the same effect as TBA value in the aggregate. In addition, The relationship between $TBA_{37^{\circ}C-2hrs}$ of the minced flesh and their lipid oxidation during storage at -$5^{\circ}C$ for 4 weeks was observed(r=O.98). $TBA_{37^{\circ}C-2hrs}$ can be expressed as the susceptibility to lipid oxidation of minced mackerel pike flesh during storage. In the results, the antioxidative effect of alcohol extract of ginger on mackerel pike flesh was observed.

• 예술인문사회 융합 멀티미디어 논문지
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• 제7권10호
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• pp.703-711
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• 2017

Today, men's appearance is an important part of the global fashion industry, including Korea, with such fashion concepts as the Metrosexual and Ubersexual. Domestic and overseas beauty and cosmetics related organizations are using this phenomenon for marketing and researching to improve the function of cosmetics. The purpose of this study was to investigate the effect of Prunus persica L. Batsch leaf extract on the skin condition after clinical application to male subjects with oily skin to determine the possibility of functional cosmetics utilizing this extract. The cleansing bars were prepared by the MP method and the cleansing packs were prepared using natural ingredients. After 8 weeks of application, moisture, oil, erythema, melanin, pH, elasticity and tactile sensation were measured using MPA5. The results showed that there was a statistically significant difference in T-zone moisture and T-zone oil after using the natural cleansing bar, but there was no significant difference between the two groups for other items. However, the statistically significant changes were found in the individual skin condition change items of the subjects in each group, and it was judged that the two kinds of formulations could be used at the same time to have a higher skin improvement effect. Thus, Prunus persica L. Batsch leaf extract demonstrates potential to be used as a functional cosmetics item.

• International Journal of Industrial Entomology and Biomaterials
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• 제5권2호
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• pp.183-188
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• 2002

Anti-fungal potential of 5 plant extracts viz., Eucalyptus citriodora, Allium sativum, Cassia sophera, Chromolaena odorata and Datura metel on the growth of mulberry brown leaf spot pathogen Myrothecium roridum were examined. Except fur the aqueous extract of Allium bulb, ethanolic leaf extract of all other plants more efficiently reduced the colony growth of the fungus on potato-dextrose-agar, Of which, Allium and Eucalyptus extracts were more effective. Initiation of radial growth of M. roridum on solid media was deferred maximum 6 days by ethanolic Eucalyptus extract and 4 days by aqueous Allium extract at $0.4 mg.ml^{-1}$. In the liquid media amended with Eucalyptus extract ($0.4 mg.ml^{-1}$) complete inhibition of sporulation was noticed upto 8 days, and initial inhibition of mycelial bio-mass generation was considerably diminished with time and reduction was 1.3 fold 14 days after application. While, complete inhibition of mycelial growth for 6-14 days was recorded with $\geq$0.1 mg.ml$^{-1}$ commercial eucalyptus oil. However, rejuvenation of growth appeared when fungus was re-inoculated in fresh media. Post-inoculate application of different doses Of Eucalyptus and Allium extracts significantly (p < 0.05) reduced the disease severity in pot-ted mulberry. However, persistence of the effect up to 28 days was apparent at $\geq$ 1.0 mg.ml$^{-1}$ and effectively was on par with carbendazim (1 mg.ml$^{-1}$ ). Almost equal control ability of 1.0 mg.ml$^{-1}$ Eucalyptus extracts can be achieved by ca. 10 times lowered dose of commercial eucalyptus oil. It seems, the toxic principle of E. citrodora to M. roridum is fungistatic in nature and may have essential oil based origin.

• Journal of Dental Anesthesia and Pain Medicine
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• 제21권4호
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• pp.329-336
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• 2021

Background: In the field of dentistry, topical anesthetics play an important role in reducing pain during needle pricks. The anesthetic property of betel leaves remain unexplored, even though they have been widely used for the treatment of various ailments. The purpose of this study was to compare and evaluate pain perception following topical application of lignocaine gel, clove gel, ice, and newly developed betel leaf extract gel during intraoral injection in children. Methods: Sixty children aged 6-10 years who met the inclusion criteria were divided into four groups. Topical anesthetic agents, 2% lignocaine (Lox-2% Jelly, Neon, Mumbai, India), 4.7% clove gel (Pain Out Dental Gel, Colgate Palmolive India Ltd, Solan, India), 10% betel leaf extract gel, and ice were applied to each group for one min, followed by administration of infiltration anesthesia. Pain perception was analyzed during needle insertion. The Wong Bakers FACES pain rating scale (WBFPRS) was used for subjective assessment and the Sound, Eye, Motor (SEM) scale for objective assessment. Recorded values were tabulated and subjected to appropriate statistical analysis using SPSS software with a P value set at 0.05. Results: The clove oil and betel leaf groups demonstrated the highest WBFPRS scores, followed by the ice and lignocaine groups. The clove, betel leaf extract, and ice groups showed equal and highest SEM scores, followed by the lignocaine group. The mean WBFPRS and mean SEM scores were statistically significant. Conclusion: Betel leaf extract gel is effective in reducing pain and can act as an alternative topical anesthetic agent.

• 한국식품과학회지
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• 제30권2호
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• pp.266-271
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• 1998

천연물로부터 안전성과 안정성이 우수한 항산화성 물질을 찾기 위하여 생약류 10종을 선정하여 95% ethanol으로 추출하면서 추출 수율과 팜유를 기질로한 항산화력을 비교한 결과 울금이 가장 좋은 것으로 나타났다. 이러한 울금 추출물을 팜유에 대해 첨가농도를 $0.05{\sim}0.5%$까지 높혀 가면서 현재 사용되는 천연 항산화제인 mixed tocopherol, rosemary 추출물들과 비교하여 항산화력을 측정한 결과 0.05%까지는 3종류 모두 유사한 항산화 효과를 나타내었으나 그 이상의 농도에 있어서는 울금 추출물의 항산화력이 월등히 우수하였다. 돈지에 대한 울금 추출물의 항산화 효과는 0.01% 첨가시 AI가 4.59로서 1.57인 팜유보다 월등하였으며, 대두유에 대해서는 첨가에 따른 항산화 효과가 전혀 나타나지 않았다. 각종 지방산 메틸에스테르에 울금 추출물을 각각 0.05%, 0.1% 첨가하였을 때 oleic acid methyl ester에 대해서는 AI가 각각 3.01, 4.33으로 크게 증가하였으나, linoleic acid methyl eser에 대해서는 각각 0.85, 0.96으로서 오히려 감소하는 경향이 나타났다.

• Advances in Traditional Medicine
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• 제8권2호
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• pp.187-191
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• 2008

Trema orientalis Linn. is commonly grown in many parts of Bangladesh. Its leaves have been used for analgesic and anti-diarrhoeal activity in traditional medicine. This study evaluates the potential analgesic and anti-diarrhoeal activity of methanol and aqueous extracts of leaves in experimental acetic acid induced writhing and castor oil induced diarrhoea in mice. The aqueous extract of leaves showed significant (P < 0.001) analgesic effect in acetic acid induced writhing in mice at a dose of 500 mg/kg body weight In castor oil induced antidiarrhoeal screening both extract increased latent period (P < 0.025) and decrease the number of stool (P < 0.025) at the dose of 500 mg/kg body weight comparable with that of the standard drug loperamide. The results provide a support for the use of this plant in traditional medicine and suggest its further investigation.

• Asian Pacific Journal of Cancer Prevention
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• 제16권2호
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• pp.761-767
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• 2015

Background: In this study, we used Daucus carota oil extract (DCOE) to target acute myeloid leukemia (AML) cells. All the AML cell lines tested were sensitive to the extract while peripheral mononuclear cells were not. Analysis of mechanism of cell death showed an increase in cells positive for annexinV and for active caspases, indicating that DCOE induces apoptotic cell death in AML. Inhibition of the MAPK pathway decreased sensitivity of AML cells to DCOE, indicating that cytotoxicity may be dependent on its activity. In conclusion, DCOE induces selective apoptosis in AML cells, possibly through a MAPK-dependent mechanism.

• 한국가금학회지
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• 제45권3호
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• pp.183-191
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• 2018

본 시험은 Salmonella gallinarum에 대한 Lactobacillus, medicinal plants extract, essential oil 급여 시 산란계의 생산성, 맹장 내 미생물 균총, 조직중량 및 혈구 조성에 미치는 영향을 조사하기 위하여 수행하였다. 공시동물로 65주령 산란계(Hy-line brown) 총 100수를 공시하였고, 시험처리는 항생제 무첨가구(NC)와 항생제 첨가구(PC)를 대조구로 하였으며, 유산균 0.1% 첨가구(LB), 약용식물 추출물 100 ppm 첨가구(MPE) 및 식물 추출물 100 ppm 첨가구(EO)를 처리구로 두었다. 배치 후 1주일동안 시험 사료를 급여하고, 이후 Salmonella gallinarum을 경구투여하였다. 조사항목으로는 생산성, 맹장 내 미생물 균총, 조직중량 및 혈구 조성을 조사하였다. Salmonella gallinarum를 경구투여한 처리구와 투여하지 않은 처리구의 경우, Salmonella gallinarum를 경구투여한 처리구가 산란율, 1일 산란량 및 사료섭취량이 다소 감소하는 경향을 보였고, 유산균(LB), 약용식물 추출물(MPE) 및 식물추출물(EO)을 급여하였을 때 약용식물 추출물(MPE)을 급여한 처리구가 대조구에 비해 다소 개선되었지만 처리구간의 통계적인 유의차는 없었다. 맹장 내 미생물의 경우, Salmonella gallinarum을 경구투여하였을 때, Salmonella 수가 유의하게 증가하였으나(P<0.05), coliform bacteria 및 유산균 수에는 영향을 미치지 않았으며, Salmonella gallinarum과 유산균(LB), 약용식물 추출물(MPE), 및 식물추출물(EO) 사이의 상호작용도 나타나지 않았다. 조직중량에서도 Salmonella gallinarum 경구투여와 유산균(LB), 약용식물(MPE) 및 식물추출물(MPE) 첨가에 대한 통계적인 유의차는 없었고, 혈구 조성에서도 Salmonella gallinarum 경구투여와 유산균(LB), 약용식물 추출물(MPE) 및 식물추출물(EO) 첨가에 대한 유의차는 없었지만 각각 총 백혈구 수가 증가하는 경향을 보이기도 하였다.

• 한국산학기술학회논문지
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• 제13권11호
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• pp.5637-5645
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• 2012

폐기되는 홍어껍질을 기능성 식품원으로 재이용하고자 홍어껍질 물 추출물의 추출특성과 세 종류의 식용유에 대한 산화억제 효과를 측정하였으며, 추출 콜라겐의 겔화를 위한 최적조건 및 겔화제 선정, 겔의 저장성, 강도와 관능평가를 실시하였다. $50^{\circ}C$ 추출물의 방향족화합물 함량과 페놀성 화합물 함량은 $25^{\circ}C$ 추출물에 비하여 각각 49.4%와 32.7% 높았으며, $50^{\circ}C$ 추출물의 환원력은 $25^{\circ}C$ 추출물 보다 52.74% 높았으나 ascorbic acid의 14.9%, BHT의 27.8%였다. 추출물의 전자공여능은 방향족 화합물과 페놀성 화합물의 함량이 높을수록 높게 측정되었다. 식용유에 대한 $50^{\circ}C$ 추출물의 산화억제 효과는 옥수수 배아유, 대두유 및 올리브유 순이었으며, 식용유 종류에 따라 ascorbic acid의 38.27~96.83%, BHT의 49.53~75.31%였다. 홍어껍질 추출물로부터 콜라겐 겔화의 최적 추출조건은 2.5배의 가수비에서 $100^{\circ}C$, 2시간이었으며, 조미하지 않은 경우에 비해 10% 조미한 경우 겔의 강도를 50%이상 저하시킬 수 있었고, 5개항에 걸친 관능평가 결과 상당한 개선을 보여 식품으로서의 제품화가 가능한 것으로 평가되었다.