The efficiency of the synthetic magnesium silicate used in basic polyols and edible oil purification is evaluated by its purification ability and filtration rate and is affected by the particle size and surface area of magnesium silicate. In this study, it was investigated the change on the particle size of magnesium silicate was influenced by the reaction temperature, injection rate, injection order (Si, Mg) and Mg/Si reaction mole ratio. The synthesized magnesium silicate was compared and analyzed for the synthesis, grinding, and refining processes. In the synthesis process, the reaction temperature and feed rate did not affect the average particle size change of magnesium silicate, while the reaction molar ratio of Mg / Si and the order of injection acted as main factors for the change of average particle size. The average particle size of magnesium silicate increased by 8.7 ㎛ from 54.4 ㎛ to 63.1 ㎛ at Mg injection when Mg molar ratio increased from 0.125 to 0.500, and increased by about 4.8 ㎛ from 47.3 ㎛ to 52.1 ㎛ at Si injection. The average particle size according to the order of injection was 59.1 ㎛ for Mg injection and 48.4 ㎛ for Si injection and the difference was shown 10.7 ㎛, therefore the filtration rate was about 2 times faster under the condition of Mg injection. That is, as the particle size increases, the filtration time is shortened and washing filtration rate can be increased to improve the productivity of magnesium silicate. The cake form of separated magnesium silicate after filtration becomes a solid through drying process and is used as powdery adsorbent through the grinding process. As the physical strength of the dried magnesium silicate increased, the average particle size of the powder increased and it was confirmed that this strength was affected by the reaction molar ratio. As the reaction molar ratio of Mg / Si increased, the physical strength of magnesium silicate decreased and the average particle size after grinding decreased by about 40% compared to the average particle size after synthesis. This reduction of strength resulted in an improvement of the refining ability due to the decrease of the average particle size and the increase of the amount of fine particle after the pulverization, but it resulted in the decrease of the purification filtration rate. While the molar ratio of Mg/Si was increased from 0.125 to 0.5 at Mg injection, the refining ability increased about 1.3 times, but the purification filtration rate decreased about 1.5 times. Therefore, in order to improve the productivity of magnesium silicate, the reaction molar ratio of Mg / Si should be increased, but in order to increase the purification filtration rate of the polyol, the reaction molar ratio should be decreased. In the synthesis parameters of magnesium silicate, the order of injection and the reaction molar ratio of Mg / Si are important factors affecting the changes in average particle size after synthesis and the changes of particle size after grinding due to the changes of compressive strength, therefore the synthetic parameter is an important thing that determines productivity and refining capacity.
This study was designed to investigate the possibility of the use of dried mackerel, which is enriched with docosahexaenoic (22:6n-3, DHA) and eicosapentaenoic (20:5n-3, EPA) acids, as a means of increasing the intake of these n-3 polyunsaturated fatty acids, and to investigate the effect of the consumption of mackerel on tissue triglyceride, cholesterol contents and fatty acid compositions. Twenty male mice were fed on 5% palm oil (control group) and 5% dried mackerel (mackerel group) diets for four weeks. Total triglyceride, cholesterol and LDL-cholesterol levels tended to decrease in serum, heart and kidney of the mackerel group compared to the control group, while HDL-cholesterol levels were increased with the intake of mackerel. In fatty acid compositions of serum and heart, the mackerel group showed increased percentages of n-3 fatty acids, especially DHA and EPA, and decreased percentages of arachidonic (20:4n-6, AA) and docosapentaenoic (22:5n-6, DPAn-6) acids compared to the control group (p<0.05). There was a similar tendency in kidney and adipose tissue but AA levels were not significantly different between the control and mackerel groups. These results indicate that intake of dried mackerel as the n-3 vehicle for increasing tissue n-3 fatty acids may be associated with improvement in lipid metabolism.
Morphological development from egg to juvenile stages of the trident goby, Tridentigertrigonocephalu5 were observed in the laboratory at Pusan, Korea. The ripe eggs were spherical in shape, measuring 0.49-0.62 mm in diameter. The eggs became ellipsoid shape after the insemination and measured about 1.40-1.58 mm on the long axis. Hatching began about 158 hours after insemination at water temperature of $20.5-24^{\circ}C$. The newly hatched larvae were 2.88-3.14 mm in total length(TL), with 27-28(10+ 17-18) myomeres. Many melanophore and guanophores are distributed on eye cups, gas bladder, optic vesicle, intestine and the caudal region. Three days after hatching the yolk and oil-globule was completely absorbed and the larvae attained a total length 3.26-3.62 mm. The larvae swam actively in the aquarium and fed on the rotifer. Ten days after hatching, the larvae averaged 5.20 mm in TL and the caudal notochord flexed at $45^{\circ}$. Twenty days after hatching, the larvae averaged 7.47 mm in TL and rudimental anal, second dorsal, caudal and pectoral fins were formed. The larvae attained 12.05-12.65 mm in TL thirty five days after hatching and are found to transit the bottom-life, and first dorsal and ventral fins are completely formed. The larvae reached the juvenile stage at 45-50 days after hatching and attained 15.85-16.95 mm in TL, and all scales appeared on the body.
Spawning behavior of the Takifugu pardarlis (Temminck et Schlegel) was observed on the Jook-do coast in Tongyong from March 1997 to June 1999. The spawning ground was locted in the intertidal zone between Tongyong and Koje-do. Its bottom was mainly gravels and stones, and its depth was 0.5~1.0 m. Spawning season was from the end of the March to the middle of May. During the spawning season, the mature fishes formed school a of 10~30 individuals, then moved to the spawning ground together. When a mature female spawned eggs, the attendant males fertilized them at the same time. The fertilized eggs obtained from the parent fishes caught at the spawning ground were adhesive, opaque and spherical, measuring 1.14~1.24 mm (mean 1.19 mm, n = 50) in diameter with numerous tiny oil globules. Hatching period was about 205 hours after fertilization at water temperature of $18.0{\pm}0.5^{\circ}C$. The newly hatched larvae were 2.92~3.10 mm (mean 3.01 mm, n = 20) in total length (TL), had a large yolk, and 11~13+14~15 = 25~28 myomeres. At 5 days, the larvae had attained 3.79~3.85 mm (mean 3.82 mm, n = 20) in TL and had transformed into the postlarval stage. At 15 days, the postlarvae had attained 7.78~7.90 mm (mean 7.84 mm, n = 20) in TL. At 21 days, had larvae attained 10.15~10.27 mm (mean 10.21 mm, n = 20) in TL and had reached the juvenile stage. All fins were formed with a complete set of fin rays having the following counts: dorsal fin rays 11~12; anal fin rays 9; pectoral fin rays 14~15; caudal fin rays 11~12.
This study was conducted to observe the early life history of sea bass, Lateolabrax japonicus. The fertilized eggs were spherical in shape and turned out to be separative and floated. Their membrane and yolk having 1~5 oil globule were transparent. Fertilized eggs were measured to be 1.33~1.46 mm in diameter. Hatching of eggs were started at 74 hrs 15 mins, 54 hrs 55 mins, 50 hrs 45 mins, after fertilization in water temperature $16.0^{\circ}C$, $18.0^{\circ}C$, $20.0^{\circ}C$ respectively. The newly hatching larvae were 3.79~3.97 mm in total length with 35~37 myomeres, and mouth and anus were closed. Melanophores were distributed on the up side of head, upper jaw and margins of the body. The 5 days after hatching larvae measured 4.78~5.24 mm in total length, yolk were completely absorbed, and transformed to postlarval stage. In this time, mouth of larvae was opened, and also melanophores were presented on the lower jaw. Head of larvae grew remarkably. The 21~22 days after hatching, total length of the larvae was 7.15~8.12 mm, the caudal fin rays began to differentiation. In 31 days after hatching, the larva were 8.46~9.16 mm in total length, and tip of the caudal notochord flexed $45^{\circ}$. The larvae reached to the juvenile stage with all the fins were developed at 61 days after hatching and attained 16.28~17.31 mm in total length.
The egg development and morphological change of larvae and juveniles of the starry flounder, Platichthys stellatus were observed in laboratory. Fertilized eggs of the species, 1.09~1.19 mm (mean $1.13{\pm}0.03mm$, n=50) in diameter, were floating, colorless, transparent in shape and lacked in oil globules. The eggs hatched out 121 hours after fertilization at water temperature $8.2{\sim}11.2^{\circ}C$. The size of the hatched larvae were 2.58~2.89 mm (mean $2.67{\pm}0.09mm$) in total length, their mouth and anus were not open yet and myotome number was 14+27=41. Melanophore and xanthophore appeared on the notochord and digestive organ and the margin of membrane fin, on the yolk sac and eyes were lacking in pigment cells. 5 days after hatching the larvae attained 4.30~4.97 mm (mean$4.74{\pm}0.21mm$) in TL, and their mouth and anus were open. 10 days after hatching the larvae transformed to postlarval stage and they were 4.67~5.75 mm in TL (mean $5.30{\pm}0.31mm$), and absorbing the yolk completely. Feeding activity increased as the mouth became larger. At 23 days, the larvae attained 6.69~8.82 mm in TL (mean $7.85{\pm}0.75mm$), and the right eye was started moving to the left side of the head. At 52 days, the juveniles attained 10.99~17.06 mm in TL (mean $13.50{\pm}1.67mm$). The right eye was moved completely onto the left side. All of the fins had completed set of the fin rays (D. 64~67: A. 45~51: P. 11: V. 6: C. 19).
A goby, Synechogobius hasta (Temminck et Schlegel) was studied to investigate the food consumed and the biological change of the food organisms, and the fish were sampled from the closed tributary and the lower Part of the Naktong River, near Pusan, during the period from November of 1967 to December of 1968. The fish were sampled from four stations (Fig. 1), the total number of fish being 1,295 and they were grouped and analysed monthly. The content of the alimentary canal was analysed in three categories according to modified Nilsson's method (Dahl 1962) with a slight alteration: 1) The number of each item of stomach contents was counted and the percentage of each item in proportion to the total number of food organisms is indicated by the letter 'N' representing numerical percentage in Table 2. 2) The percentage of fish which contained any items of food organisms in proportion to the total number of fish caught in a given season is indicated by the letter 'O' representing frequency of occurrence. 3) Dominant groups of food items were selected and the percentage of the number of each dominant item in proportion to the number of the food organisms belonging to the dominant groups is indicated by the letter 'D' representing dominance. All food organisms were classified in 50 food item categories and then they were grouped in 13 main groups (Fig. 2-1), and they were further divided into 1) obligatory bottom animals, 2) organic drifts and 3) actively swimming forms; according to the conditions of the animal communities within the habitat. Since the majority of its food was composed of the obligatory bottom animals ($94.6\%$), the fish appeard to be a typical bottom feeder. And the dominant food organisms of the fish is generally determined by the local composition of the benthic fauna within the fish habitat. And their seasonal rhythm occurs among the food organisms in the stomach by the biological interaction. Locality variation in the population of the same food organism occurs due to the difference of food organisms in the habitat of the fish at Seonam and Garak, and at Seongsan and Hadan the condition of the niche for the fish in the both regions seems to be the same since the composition and the seasonal variation of the organisms were the same. The results may be summarized as follows: 1) The goby mainly feed on the animals of bottom fauna, and the food organisms are deter-mined by the food compositions within the habitat. 2) Seasonal variation of the stomach content shows the seasonal rhythm due to the biological variation of the population and their interaction. 3) The goby shows no preference on specific food, and the food is composed of a variety of animals. 4) Major food items of the goby are Polychaeta, Palaemon modestus, Isopoda, Gammaridea, Insecta (nymphs and larvae), Ilyoplax deschampsi, and Paratye compressa. 5) Logitudinal succession oil the population of the food organisms is apparently recognized within the community of Seongsan, Garak and Seonam. 6) The goby begins to descend toward the estuary and sea around April when the water temperature reaches $20^{\circ}C$, and they begin to return to river waters in September.
These days, agricultural products cultivated in facilities occupy the highest percentage of agricultural output price. Specifically cucumbers have been one of the crops that farmers prefer to growing, because their prices were high. However, cucumber crop is sensitive to the soil and environments and it requires the exact crop management. In order to establish cultivation techniques for cucumbers, the current situation of cucumber cultivation was surveyed from ten cucumber farmlands; five farmlands of cucumber cultivation in forcing and five of semi-forcing practicing systems, respectably. The soil conditions were alluvial or valley in soil topology, moderately or poorly drainage in soil drainage classes, coarse loamy in soil texture family. Soil was managed with deep plowing combined with application of basal fertilizers such as compost, rice straw, oil cake, wood chip and chemical fertilizer. The whole soil was prepared in uniformly with rotary. Three major nutrients ($N-P_2O_5-K_2O$)of basal application were 815-464-529 kg $ha^{-1}$ in forcing and 197-135-151 kg $ha^{-1}$ in semi-forcing cultivation. Top dressing of fertilizer was supplied in fertigation system of macro and micro elements in 2~3 day interval with water irrigation. The average yields of cucumbers were $168t\;ha^{-1}$ with 381,000 thousand won $ha^{-1}$ in average gross profit (AGP) in forcing cultivation and $115t\;ha^{-1}$ with 177,000 thousand won $ha^{-1}$ in AGP in semi-forcing cultivation. Cucumber production during the winter season was considered to increase the gross profit because cucumber price tends to stay in high level during this time. The accumulation of soil chemicals like EC, available $P_2O_5$ and exchangeable cations could be controlled by rice straw application. The rice straw application increased soil temperature during the winter season, in exchange of soil air, and in extension of plant roots. In addition, the rice straw application somewhat affected decrease of salts accumulation.
Jo, Ji-Song;Nguyen, Do Quynh Anh;Yun, Jun-Ki;Kim, Yu-Na;Kim, You-Geun;Kim, Sung-Bae;Seo, Yang-Gon;Lee, Byung-Hak;Kang, Moon-Kook;Kim, Chang-Joon
Microbiology and Biotechnology Letters
/
v.37
no.3
/
pp.231-237
/
2009
This paper aimed to develop a solvent extraction and purification process to recover high-purified ${\beta}$-carotene from recombinant Escherichia coli. Cells harvested from the culture broth were treated through numerous steps: dehydration, solvent extraction, crystal formation and separation. To optimize the extracting condition, experiments were carried out to investigate the effect of cell disruption, temperature, organic solvents, solvent-biomass ratio on the yield of ${\beta}$-carotene extracted from cells. The result indicated that no significant differences of extraction yield were observed from cells with or without step of cell disruption. Among different extracting solvents, the highest extraction yield of ${\beta}$-carotene, 30.3 mg-${\beta}$-carotene/g-dry cells, was obtained with isobutyl acetate at solvent-biomass ratio 25 mL/g-dry cells at $50^{\circ}C$. Notably, in case of acetone, the extraction yield was quite low when using acetone itself, but increased almost up to the highest value when combining this solvent and olive oil. The purity of ${\beta}$-carotene crystals obtained from crystallization and separation was 89%. The purity degree was further improved up to 98.5% by treating crude crystals with additional ethanol washing.
Jo, WooJin;Jeong, SooHwa;Park, SungJin;Choi, YoungTai;Lee, DongHyun
Korean Chemical Engineering Research
/
v.53
no.6
/
pp.783-791
/
2015
A fluidized-bed reactor with an inside diameter of 0.1 m and a height of 1.2 m was used to study the effect of steam and catalyst additions to air-blown biomass gasification on the production of producer gas. The equipment consisted of a fluidized bed reactor, a fuel supply system, a cyclone, a condenser, two receivers, steam generator and gas analyzer. Silica sand with a mean particle diameter of $380{\mu}m$ was used as a bed material and calcined dolomite ($356{\mu}m$), which is effective in tar reduction and producer gas purification, was used as the catalyst. Both of Korea wood pellet (KWP) and a pellet form of EFB (empty fruit bunch) which is the byproduct of Southeast Asia palm oil extraction were examined as biomass feeds. In all the experiments, the feeding rates were 50 g/min for EFB and 38 g/min for KWP, respectively at the reaction temperature of $800^{\circ}C$ and an ER (equivalence ratio) of 0.25. The mixing ratio (0~100 wt%) of catalyst was applied to the bed material. Air or an air-steam mixture was used as the injection gas. The SBR (steam to biomass ratio) was 0.3. The composition, tar content, and lower heating value of the generated producer gas were measured. The addition of calcined dolomite decreased tar content in the producer gas with maximum reduction of 67.3 wt%. The addition of calcined dolomite in the air gasification reduced lower heating value of the producer gas. However The addition of calcined dolomite in the air-steam gasification slightly increased its lower heating value.
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