• Title/Summary/Keyword: Offsprings

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Effect of Subtotal Pancreatectomy of Female Mice on the Embryo and Offspring (암컷 생쥐의 췌장 부분절제가 배아와 신생자에 미치는 영향)

  • Cho, Young-Jae;Lee, Jung-Sun;Gu, Sun-Kyung;Park, Moon-Nyeo;Lee, Ha-Kye;Cho, Byung-Nam
    • Development and Reproduction
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    • v.10 no.3
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    • pp.185-192
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    • 2006
  • It is known that maternal diabetes induces a premature delivery and a congenital malformation. Although it was suggested that hyperglycemia is a main cause, little has been known. The effects of subtotal pancreactomized animal as a diabetic model on the embryo and the offspring were investigated. 1) Although survival rate of offsprings from the 90% pancreactomized female was lower than that of control, body weight of the survived offsprings was similar to that of control until 6 weeks after birth. When the liver was investigated, abnormal tissue was observed in both female and male offsprings from the pancreactomized mother. The level of major urinary protein(MUP) that is synthesized in the liver and secrets into urine was decreased in both female and male, supporting the liver abnormality. 2) In the pancreactomized female, size of the Langhan's island was increased and the liver also was observed abnormal. However, blood glucose level of the pancreactomized mother was not different from that of control. The low body weight and survival rate of offsprings from the $inhibin-{\alpha}$ overexpressed mice was very similar to those of the offsprings from diabetic mother. Thus when the mother itself was investigated after DNA injection, blood glucose level in the $inhibin-{\alpha}$ overexpressed mice was not different from that of control although size of the Langhan's island was decreased. Taken together, subtotal pancreactomy affected the offspring and embryo and influenced deeply the mother itself. However glucose level of the mother was not changed during investigation. It suggested that the relationship between the diabetic mother and embryo through offspring is very complex.

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A Study on the College Student's Awareness of Supporting the Elderly in Pusan (부산시내 일부 대학생들의 노인 부양의식에 관한 연구)

  • 김수혜;김병성;박형종
    • Korean Journal of Health Education and Promotion
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    • v.10 no.2
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    • pp.56-72
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    • 1993
  • In our society the value system has changed to individualized one, so that the awareness of supporting the elderly is changing now. This study aims to provide basic materials for the welfare policy for the elderly and to foresee the probability of maintenance and development that the most ideal system of supporting the elderly by analyzing the male and female students' attitude toward the elderly and the degree of their awareness of supporting the elderly according to several factors. A questionnaire was developed with this purpose, the study subjects of this study were 365 college students who were recruited from 6 colleges(179 male students and 186 female students) living in Pusan, and the study period was from November 9. to November 23. 1992. The results were as follows: 1. In the factors related to attitude toward the elderly, male students had more positive attitude than female students toward the desire of living with their parents in the future(P=0.000). 2. In the expectant awareness of being supported by their offsprings and the desire of living together with their offsprings male students were more highly than female students(P=0.000). 3. The subjects who want to live together with their parents in the future desired more to live with their offsprings in the period of old age(P=0.000). 4. As results of hypotheses verification about the awareness of supporting the elderly, there were significant differences according to the relationship between mother-in-law and daughter-in law(P=0.001), the receptive attitude toward the elderly(P=0.000) and the desire of living with their parents in the future(P=0.000) 5. In the correlations between all variables and the awareness of supporting the elderly, the economical and emotional awareness of supporting the elderly had positive correlations with the relationship between mother in-law and daughter-in-law and their parents' filial piety to grandparents(P<0.01), and service awareness of supporting the elderly had positive correlations with age, sex, the relationship between mother-in-law and daughter-in-law and their parents' filial piety to grandparents(P<0.01). In the correlations between each of the awareness of supporting the elderly, the correlations between each one had high positive correlations(P<0.001).

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Effect of Maternal Dietary Restriction on Serotonin and Catecholamine Levels of the Developing Rat Brain (어미쥐의 식이섭취제한이 이유전 새끼쥐 뇌의 Seretonin과 Catecholamine 수준에 미치는 영향)

  • Chang, Kyung -Ja;Choi, Hay-Mie
    • Journal of Nutrition and Health
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    • v.18 no.1
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    • pp.23-28
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    • 1985
  • A quantitative restriction of maternal diet was given to the Sprague Dawley rats during the third week of gestation and during the lactation Dams of deficient groups were fed 20g of a stock diet a day and compared with the control group fed ad libitum throughout the experimental Period Dietary restriction started from birth in deficient 1 group and from the 14th day of gestation in deficient 2 group. Brain serotonin, norepinephrinet dopamine, and tyrosine of offsprings were determined at several intervals. Brain serotonin of offsprings of deficient groups 1 and 2 were significantly lower than the control and significant differences between the deficient greeds were noticed Brain catecholamines and tyrosine of offsprings of deficient groups were significantly lower than the control and there was no difference between the deficient groups. Maternal dietary restriction during the gestation and the lactation may have retarded the synthesis of serotonin and catecholamines in the brain.

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Production of cloned Rabbits Embryos and Offsprings by Nuclear Transplantation using In Vitro Matured Oocytes in Rabbits (토끼의 체외배양 난자를 이용한 핵이식으로 복제수정란 및 복제산자의 생산)

  • 박충생;전병균;이경미;윤희준;이효종;곽대오;최상용
    • Journal of Embryo Transfer
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    • v.10 no.1
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    • pp.65-72
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    • 1995
  • The purposes of this study were to produce cloned rabbit embryos and offsprings by nuclear transplantation(NT) using in vitro matured oocytes as nuclear recipient cytoplasm and to determine the effect of frozen nuclei donor embryos on the production efficiency of cloned embryos. The 8cell embryos were collected from the mated does by flushing oviducts with Dulbecco's phosphate buffered saline containing 10% fetal calf serum(FCS) at 40 hours after hGG injection. A portion of collected embryos were preserved at 4$^{\circ}C$ for 24 hours and a portion of them were frozen by vitrification method. The embryos used for donor nuclei were synchronized in the phase of Gi /S transition. The in vitro matured oocytes were used as recipient cytoplasm following removing the nucleus and the first polar body. The synchronized blastomeres from fresh, cooled or frozen embryos were injected into the enucleated oocytes by micromanipulation and were electrofused by electrical stimulation of three pulses for 60 $\mu$sec at 1.0 W /cm in 0.28 M mannitol solution. The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10% FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$incubator. Following in vitro culture of the NT embryos to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The nuclear transplant embryos developed in vitro to 2- to 4-cell stage were transferred into the oviducts of synchronized recipient does. The results obtained were summarized as follows: 1. The fusion rates of the blastomeres from fresh, cooled and frozen embryos with the in vitro matured and enucleated oocytes were 100, 95.8 and 64, 3%, respectively. 2. Development in vitro to blastocyst was significantly(p<0.05) different between the cloned embryos with the blastomeres from fresh, cooled or frozen embryos as 39.0, 20. 9 and 15.7%, respectively. 3. The mean numbers of cell cycle per day during in vitro culture of cloned embryos blastomeres from fresh, cooled or frozen embryos was 1.31, 1.29 and 1.16, respectively. 4. A total of 77 nuclear transplant embryos were transferred into 6 recipient does, of which two offsprings were produced from a foster mother 31 days after embryo transfer.

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Effect of Food and Salinity on Larval Growth and Survival of the River Puffer, Takifugu obscurus (황복, Takifugu obscurus의 초기 발달 동안 성장 및 생존에 있어 먹이와 염분의 효과)

  • 강희웅;강덕영;조기채;이진호;박광재;김종화
    • Journal of Aquaculture
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    • v.17 no.3
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    • pp.221-227
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    • 2004
  • In the present study, we investigated the effects of food and salinity on growth and survival of river puffer, Takifugu obscurus offsprings in indoor land-based tank during the early development. In the food experiment, the river puffer larvae (TL 8.7$\pm$0.1 mm, BW 20.0$\pm$3.2 mg) were fed with tubificid, Limnodrilus gotoi, water flea, Daphnia carinata, mysid, Neomysis awatschensis, Manila clam, Ruditapes philippinarum and artificial food together Artemia nauplii for 30 days, and the growth and the survival of larvae were investigated. In the salinity experiment, the river puffer larvae and juvenile at three stages (Stage I: TL 5.8$\pm$0.4 mm; Stage II: TL 12.0$\pm$0.2 mm, Stage III: TL 44.5$\pm$0.7 mm) were supplied with Artemia nauplii, water flea and/or artificial food and were reared in 0, 10, 20 and 30 psu at respective stages. The results shows that the growth rates of larvae feeding with artificial food and tubificid together Artemia nauplii were higher than those of larvae in other groups, but the survival rates of larvae feeding with water flea and mysid together Artemia nauplii were higher than those of larvae in other groups. In salinity, the results shows that the growth and survival rates of offsprings were significantly higher in 10∼20 psu than those in 0 and 30 psu at Stage I and n. At Stage III, the growth and the survival rates were the highest in 20 psu, but the lowest in 0 psu. However, it should be noted that the rates in 0 and 30 psu were significantly enhanced at Stage III in comparison with those at Stage I and II. Therefore, it is concluded that river puffer, T. obscurus is euryhaline marine species, which can normally grow and live at salinities from 0 to 30 psu, and that a combinative supplement with Artemia nauplii and water flea, D. carinata may confer an advantage on growth and survival of the river puffer offsprings in indoor land-based tank.

Optimal Salinity and Temperature Conditions for the Growth of the Ultra-small Rotifer Synchaeta kitina (초소형 Rotifer Synchaeta kitina의 성장을 위한 최적 염분 및 수온 조건)

  • Park, Jin-Chul;Park, Heum-Gi
    • Journal of Aquaculture
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    • v.21 no.2
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    • pp.70-75
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    • 2008
  • We investigated the optimum salinity and temperature conditions for mass culture of ultra-small rotifer Synchaeta kitina. In the salinity experiment of ranging within 5 and 30 psu, the population growth of S. kitina increased continuously up to 20 psu, and then decreased over 20 psu. Their maximum density showed 390.1 inds./mL at 5 psu. A pre-reproductive phase was shortened in low salinity than high salinity. Also, the maximum offsprings and maximum lifespan and lifespan of the female were 13.4 inds. and 5.9 days, respctively at 5 psu. In the temperature experiments of ranging within 16 and $32^{\circ}C$, the population growth of S. kitina increased continuously up to $24^{\circ}C$, and then decreased over $24^{\circ}C$. The highest maximum density showed 492.8 inds./mL at $16^{\circ}C$. Their offsprings increased significantly with temperature decrease, and the maximum number of offsprings per female was 9.2 females. at $16^{\circ}C$. Their lifespan increased with temperatures decrease and the longest lifespan was to 5.5 days at $16^{\circ}C$. From these results, we conclude that the optimum culture conditions of S. kitina is 5 psu and $16^{\circ}C$.

Effect of Temperatures on the Growth of Susceptible and Malathion Resistant Green Peach Aphid Strains (Malathion 저항성 및 감수성 복숭아혹진딧물의 온도에 따른 발육특성)

  • Seung Seok Song;Naoki MOTOYAMA
    • Korean journal of applied entomology
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    • v.35 no.4
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    • pp.297-301
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    • 1996
  • This test has been carried out to evaluate the effect of temperature on the growth of the insecticide susceptible strain, URY-O nomal genotype and insecticide resistant strain, O-RT abnormal genotype, and ABURABI nomal genotype. The nymphal periods were not significantly different between URY-O and O-RY strains at $25^{\circ}C$. At $30^{\circ}C$, susceptible strain URY-O could give birth to offsprings almost nomally, while resistant strain O-RY could not produce any offspring for 20 days which results in nymphal death. The numbers of offsprings of strain URY-O and strain ABURABI were not different between $25^{\circ}C\;and\;28^{\circ}C$, but strain O-RY, when it was reared at $28^{\circ}C$, could produce offsprings only 10% of those at $25^{\circ}C$. Body weight of strain URY-O and strain ABURABI were 0.22mg/female and 0.27mg/female, respectively at $28^{\circ}C$, however that of O-RY was only 0.16mg/female, showing considerable difference between normal and abnormal genotype. Substrain O-RY(+) which has high esterase activity showed poor reproduction ability(0.8 progenies per $G_{1}$ individual than substrain O-RY(-) (3.4 progenies per $G_{1}$ individual) which has low esterase activity at $28^{\circ}C$

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Prenatal Treatment Effects of Oriental Herbal Medicine Kamijadowhan on Developmental and Reproductive Toxicity in Rats

  • Park, Young-Jin;Kim, Jung-Ran;Ryu, Jae-Chun;Shim, Bum-Sang;Park, Seung-Hoon;Kwon, Oh-Seung
    • Environmental Mutagens and Carcinogens
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    • v.21 no.2
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    • pp.77-81
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    • 2001
  • Kamijadowhan (KMD), an oriental herbal medicine used for anti-angiogenic effect, was extracted with 80% ethanol from mixture of source materials and lyophilized. KMD was orally administered to plugpositive pregnant rats from gestational days 12 to 20, dividing into three groups including vehicle-treated control, 0.5 g/kg or 3 g/kg KMD-treated groups. Dam weight during gestation and post-gestation, weight of pre- and post-weaning offsprings in male and female, and reproductive and developmental endpoints including incisor eruption, eye opening and testes descent were measured. No significant alterations in development of physical landmarks in offspring, maternal weight gain during gestation and post-gestation, and offspring weight were observed in KMD-treated group. The measurement of organ weight at post-gestational days 21 was not changed in dams. In 0.5 g/kg KMD-treated rats, kidney weights in male and female offsprings were significantly increased, and the body weight in male offspring was also increased. Liver and brain weights were not changed. Taken together, these data suggest that KMD may not significantly cross the placenta and produce no reproductive and developmental toxicity at maternally non-toxic dosages.

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Sex Reverse in Teleost Culture II. Sex Reversal of Guppy and Tilapia by $17\beta$-Estradiol and $17\alpha$-Methyltestoserone (경골어류의 성전환에 관한 연구 II. $17\beta$-Estradiol 및 $17\alpha$-Methyltestosterone을 이용한 Guppy 및 Tilapia의 성전환에 관한 연구)

  • 윤종만;박홍양
    • Korean Journal of Animal Reproduction
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    • v.13 no.1
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    • pp.40-48
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    • 1989
  • Guppy fry were treated for the first 40 days of life with 0, 20, 40, 60 & 100$\mu\textrm{g}$ of estradiol per gram of food in order to change the sex of normal males to functional females(genetic male). The present investigation deals with the effects of steroid hormones, such as $\beta$-estradiol and testosterone, on the sex differentiation in guppy and tilapia. The results obtained were summarized as follows. 1. In B (20$\mu\textrm{g}$/g diet) group 17$\beta$-estradiol-treated, 67.8% of male offsprings were produced. 2. In D (60$\mu\textrm{g}$/g diet) group 17$\beta$-treated, 67% of female offsprings were produced. 3. B, D groups of genetic male brooders had significantly different effects (P<0.01) upon sex ratios of their progeny. 4. This strongly indicates that sex direction has been achieved and that the male is the heterogametic sex. 5. The group that produced the highest percentage of male offspring(male percentage of observed number to expected number was 91%) contained only full-sibling male brooders to the sex-reversed female brooders. 6. After 7 months following treatment, the sex-reversed males had ovarian portion in the anterior region and a testicular portion in the posterior region of the same intersexual gonad, respectively. 7. At 7 months after treatment, the ovareis revealed a complete arrest of the ovarian formation, and appearances of spermatogenetic cell cysts among surviving auxocytes. 8. In most of sex-reversed fish, anterior portion of test is was devoid of sperm ducts including the seminal vesicle and vas deferens. 9. The male transferrin showed two strong bands, while the female transferrin showed a single weak band. 10. One of the two bands of male transferrin showed the same mobility with band of female transferrin.

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Effect of endocrine disrupter, Nonylphenol and DEHP(Di-(2ethylhexyl)phatalate) on the cocoon production and the hatchability of Eisenia fetida (Ennelida: Oligochaeta) (내분비교란물질인 Nonylphenol과 DEHP(Di-(2ethylhexyl)phatalate)가 줄지렁이(Eisenia fetida)의 산란 및 부화에 미치는 영향)

  • Park, Kwang-Il;Bae, Yoon-Hwan
    • Journal of the Korea Organic Resources Recycling Association
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    • v.20 no.2
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    • pp.89-95
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    • 2012
  • This study was carried out to investigate the effects of endocrine interupter, Nonylphenol and DEHP on the cocoon production, the hatchability of cocoon and the number of offsprings per cocoon of Eisenia fetida. And the cocentrations of Nonylphenol and DEHP of sewage sludges in Pocheon city (Northeastern area of South Korea) were also investigated. Nonylphenol below the concentration of 100 mg $kg^{-1}$ did not reduce the cocoon production, the hatchability of cocoon and the number of offsprings per cocoon. DEHP above the concentration of 1,500 mg $kg^{-1}$ reduced the cocoon production, and DEHP over the concentration of 2,500 mg $kg^{-1}$ could reduce the hatchability. There was no Nonylphenol in sewage sludge of Pocheon city, but maximum concentration of DEHP was 1,640mg $kg^{-1}$, which could make the population of Eisenia fetida extinct gradually if sewage sludge of Pocheon was supplied to Eisenia fetida for a long time.