• Clinical and Experimental Reproductive Medicine
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• v.24 no.3
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• pp.393-398
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• 1997

Cytogenetic analysis was performed in 1321 couples and 141 women with history of abnormal reproductive outcome during 1988-1996. The use of high resolution banding technique and fluorescence in situ hybridization (FISH) in the chromosome analysis has made the precise evaluation of chromosome aberrations. The prevalence of balanced chromosomal translocation carriers were 3.74% (104/2783 patients). 70 cases (2.52%) were reciprocal translocation carriers and 34 (1.22%) had Robertsonian translocations. Chromosome aberrations were more frequent in women (73 cases) than in men (31 cases). No phenotypical abnormalities were found in all carriers, but they experienced abnormal reproductive outcomes such as recurrent spontaneous abortions, anomalous offsprings or infertility problem. Prenatal diagnosis was carried out on 36 subsequent pregnancies in balanced translocation carriers. The fetal karyotypes showed that 12 cases (33%) were normal, 22 (61%) were balanced translocations, and two (6%) were unbalanced translocations. It is concluded that the prevalence of balanced chromosomal translocations in patients with abnormal reproductive outcome is higher than that of the normal population. Most of the fetal samples showed normal karyotypes or balanced translocations. Although the incidence of chromosomal imbalance in the fetuses was relatively low in prenatal diagnosis, individuals with balanced translocations are predisposed to abnormal offspring with partial trisomy or monosomy. Therefore we recommend that genetic counselling and cytogenetic prenatal diagnosis for translocation carriers have to be offered to prevent recurrent chromosomal abnormal babies.

• Journal of the Korea Society of Computer and Information
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• v.16 no.3
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• pp.45-51
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• 2011

This paper proposes a method to accelerate the evolution speed of individuals through hybrid reproduction of monogenesis and gamogenesis. Monogenesis as a reproduction method that bacteria or monad without sexual distinction divide into two individuals has an advantage for local search and gamogenesis as a reproduction method that individuals with sexual distinction mate and breed the offsprings has an advantages for keeping the diversity of individuals. These properties can be properly used for improvement of evolution speed of individuals in genetic algorithms. In this paper, we made relatively good individuals among selected parents to do monogenesis for local search and forced relatively bad individuals among selected parents to do gamogenesis for global search by increasing the diversity of chromosomes. The mutation probability for monogenesis was set to a lower value than that of original genetic algorithm for local search and the mutation probability for gamogenesis was set to a higher value than that of original genetic algorithm for global search. Experimental results with four function optimization problems showed that the performances of three functions were very good, but the performances of fourth function with distributed global optima were not good. This was because distributed global optima prevented individuals from steady evolution.

• Journal of Embryo Transfer
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• v.12 no.3
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• pp.259-267
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• 1997

To improve the efficiency of production of cloned embryos and animals by nuclear transplantation in the rabbit, the effect of cell cycle of donor nuclei and type of recipient cytoplasm on the in vitro developmental potential and production efficiency of offspring was determined. The embryos of 16-cell stage were collected from the mated does at 48h post-hCG injection and they were synchronized to G$_1$ phase of 32-cell stage. The oocytes collected at 14h post-hCG injection were freed from cumulus cells and then enucleated. One group of the enucleated cytoplasms was activated by electrical stimulation prior to injection of donor nucleus, and the other group was not pre-activated. The separated G$_1$phase blastomeres of 32-cell stage embryos were injected into the perivitelline space of recipient cytoplasms. After culture for 20h post-hCG injection, the nuclear transplant oocytes were electrofused and activated by electrical stimulation and the fused nuclear transplant embryos were co-cultured for 120h and the nuclear transplant embryos developed to blastocyst stage were stained with Hoechst 33342 dye and their blastomeres were counted. Some of the nuclear transplant embryos developed in vitro to 2- to 4-cell stage were transferred into the oviducts of synchronized recipient does. The electrofusion rate was similar between the types of donor nuclei and recipient cytoplasms used. However, the nuclear transplant embryos using G$_1$ phase donor nuclei were developed to blastocyst at higher rate(60.3%) than those using S phase ones(24.7%). Also, when non-preactivated oocytes were used as recipient cytplasms, the develop-mental rates of nuclear transplant embryos to blastocysts were significantly(P< 0.05) higher(57.1%) than those using preactivated ones(20.8%). The cell counts of nuclear transplant embryos developed to blastosyst stage were increased signficantly(P<0.05) more in the non-preactivated recipient cytoplasm(163.7 cells), as compared whit the preactivated recipient cytoplasm(85.4 cells), A total of 49 nuclear transplant embryos were tranferrid into 5 recipient does, of which two offsprings were produced from a foster mother 31 days after embryo transfer. these results showed that the blastomeres of G1 phase and non-preactivated oocytes might be utillzed efficiently as donor nuclei and recipient cytoplasms in the nuclear transplant procedure, thought the offspring production remained still low.

• Korean Journal of Animal Reproduction
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• v.26 no.2
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• pp.95-104
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• 2002

This study was performed during the four seasons for the production of transgenic pigs containing the human erythropoietin(hEPO) transgene. Purebred Landrace gilts and sows approximately 8∼15 months of age (n=42) were used fur the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Synchronization and superovulation procedures were used that cyclic gilts were synchronized with 20mg altrenogest (ALT) per day for 9days after PG600 administration followed by superovulation with 1500IU pregnant mares serum gonadotropin (PMSG) and 500IU human chorionic gonadotrophin (hCG). Preparation of recombinant gene for microinjection is mice whey acidic protein promoter (mWAP) linked to human erythropoietin (hEPO) gene. After hormone treatment, 650 embryos were collected from 23 donors and 83.1% (540/650) embryos were in 1-cell stage which can be visualized the pronuclei for DNA microinjection. A total of 543 DNA microinjected embryos fiom donors were transferred to 19 synchronized recipients, seven of them maintained pregnancy and delivered 47 piglets. One of the 47 offsprings were determined to have transgene by PCR analysis. The overall rate of transgenic production was 2.13% (tansgenic/offspring). This study provides the success and useful information regarding production of transgenic pig for bioreactor research.

• Journal of Veterinary Clinics
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• v.12 no.1
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• pp.877-886
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• 1995

The recycling nuclear transplantation(NT) technique has the powerful potential of producing a large number of genetically identical embryos and offsprings from one embryo. Multiple generational cloning by this technique utilizes the NT embryo itself as the donor for the next generation of cloning. In this experiment, we have produced the third generational cloned embryos by recycling NT. Further we examined comparatively the electrofusion rate and in vitro developmental potential in the cloned embryos of the first second and third generations. The embryos of 16-cell stage were collected from the mated does by flushing oviducts with Dulberco's phosphate buffered saline containing 10 % fetal calf serum(FCS) at 47 hours after hCG injection. In the first generation NT, the nuclear donor embryos were synchronized in the phase of Gl/S transition of 32-cell stage. The first and second generation NT embryos developed to 16-cell were used as donor nuclei for second and third generation. The recipient cytoplasms were utilized the oocytes collected at 14 hours after hCG injection, following revoming the nucleus and the first polar body by micromanipulation. The separated blastomeres were injected into the enucleated recipient oocytes by micromanipulation and were fused by electrical stimulation. The electrofusion rate was seen to be 78.0, 88.0 and 90.3 % in the first second and third generation NT rabbit embryos, respectively. The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10 % FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. The in vitro developmental potential to blastocyst stage was significantly(P<0.05) decreased in the third(7.2 %) generation NT embryos compared to the first(53.1 %) and second(16.1 %) generation NT embryos. Following in vitro development to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The mean blastomere numbers and cell cycle numbers of NT embryos during the culture period were significantly(p<0.05) decreased in the second(93.9 cells and 6.55 cylces) and third(81.5 cells and 1.35 cylces) generation, compared to the first(189.9 cells and 7.55 cylces) generation.

• Journal of Nutrition and Health
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• v.5 no.2
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• pp.75-82
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• 1972

Fifteen cases of primiparas and their offsprings (fetal cord) were investigated with regard their serum total, free and esterified cholesterol by means of Liberman Buchard reaction. The serum ${\alpha}-and\;{\beta}-lipoprotein$ were analyzed by cellulose acetate electrophoresis, and the serum atherolipid numbers were calculated on the bases of the serum total cholesterol and ${\beta}-/{\alpha}-$ lipoprotein ratio, with the following conclusion. 1.Total, free and esterified cholesterol are $178.9{\pm}25.3$, $45.1{\pm}12.6$ and $133.7{\pm}20.6\;mg.%$ in the normal control women, $201.5{\pm}29.5,\;58.7{\pm}42.1$ and $157.1{\pm}26.2\;mg.%$ in the maternal blood, showing hypercholesterolemia in the latter as compared to the former. 2. The serum total, free and esterified cholesterol in the cord blood are $94.5{\pm}20.4$, $32.9{\pm}1.5$ and $61.2{\pm}18.9mg.%$, showing hypocholesterolemia as compared to the control women and maternal blood. 3. The serum ${\alpha}-$, $pre-{\beta}$, ${\beta}-lipoprotein$ and chylomicron are $24.2{\pm}4.2$, $17.3{\pm}3.4$, $51.8{\pm}4.8$ and $6.0{\pm}1.6%$ in the normal women, whereas $14.9{\pm}2.1$, $22.2{\pm}5.1$, $58.7{\pm}3.3 and 3.1{\pm}1.2%$ in the maternal serum, $32.4{\pm}8.1$, $28.8{\pm}2.4$, $25.8{\pm}7.0$ and $3.1{\pm}0.9%$ in the cord serum, showing $hyper-{\beta}-lipoproteinemia$ in the former and $hypo-{\beta}-lipoproteinemia$ in the latter. 4. The serum atherolipid number of the normal control women, maternal cord blood are $4.21{\pm}1.24$, $8.02{\pm}1.42$ and $1.12{\pm}0.37$, showing hyperlipemia in the former and hypolipemia in latter. 5. The relative ratio of the serum free and esterified cholestrol of both normal control women and maternal blood is about 1 : 3, while that of the fetal blood about 1 : 2. 6. The relative ratioes of the serum ${\alpha}-and$ ${\beta}-lipoprotein$ in the control women is about 1 : 2, that of materna blood about 1 : 3 and that of the fetal blood about equal magnitude. 7. The serum esterified cholesterol, ${\alpha}-lipoprotein,\;{\beta}-/{\alpha}-lipoprotein$ ratio and atherolipid number fluctuates are proportionally between the maternal and fetal blood, while the serum free, total cholesterol and ${\beta}-lipoprotein$ between the two vary inversely with statistically significant corelations. 8. It is apparent from the above results that the fetal nutritional demand for lipids resulted from hypocholesterolemia and hypo ${\beta}-lipoproteinemia$ seems to be met satisfactorily by maternal hypercholesterolemia and hyper ${\beta}-lipoproteinemia$, which seems to pose a significant maternal-infant nutritional relationship. A brief ciscussion was made on these conciusion in the light of biochemistry and endocrinology.

• Sijohaknonchong
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• v.24
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• pp.47-85
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• 2006

One of the famous Korean verses, Sijo 'In hemp clothes in winter(삼동에 베옷 입고)' is in thirty kinds of anthologies and ten kinds of Chinese writings. The major point in literary transmission of this work was the writer. The writer of this verse is Cho Sik(조식) in most anthologies. The writer of this work is recorded as Kil Jae(길재), Kim In-hoo(김인후), Kim Eung-jung(김응정), Lee Mong-gyu(이몽규) or Kim Ryung(김령) in Chinese writings. These people were famous for their studies and virtue, but they did not take office and retired from the world until they die. They were faithful to their principles serving their kings, though the country had wanted them to take a position in the government. Even though they did not in government service, they left some anecdotes and stories of lamentation after hearing their king's death. Because these stories and anecdotes can be easily connected with 'In hemp clothes in winter' which is about mourning over death of a king, they were known as the writer of this poem. Especially, their offsprings, juniors and followers often wrote these people as the writer of this work to represent their honor. Throughout the studies of several literary documents, this poem is written by Kim Eung-Jung, who lived in Kangjin(강진) in Jeonla province(전라도) and did not take office in all his life. He made this poem when he had heard the King Myoungjong(명종)'s death. Various transmission of literary documents with the records about 'In hemp clothes in winter' have a significance that can show the aspects of noble men's acceptance and transmission of Sijo.

• 한국노년학
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• v.38 no.2
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• pp.329-349
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• 2018

The objective of this study is to examine whether there are differences in participation in social activities and the effects of social activity types on the levels of happiness of the Korean older adults by age groups. The 5th wave(2014) of the Korean Longitudinal Study of Ageing (KLoSA) was used and a total of 3,273 elderly were selected from the 5th wave, who were 65 years old and older and who were not living with their offsprings. Chi-square test, ANOVA, and hierarchical multiple regression analysis were used. The rate of participation in economic activities declined with age and the levels of participation in leisure activities was higher in the young-old group in comparison to the middle- and the oldest- old groups. The levels of participation in family activities was higher in the middle- and the oldest-old groups in comparison to the young-old group. Socializing activities and religious activities measured by the frequencies of activities were not significantly different among the age groups. The results of a regression analysis of social activity types and their effects on happiness by age groups are the followings: 1) Leisure, socializing, religious, and family activities all (with the exception of economic activities) significantly affected the level of happiness of older persons in the young-old group. 2) However, only socializing and family activities in the middle-old age group, and only family activities in oldest-old group, had a statistically significant impact on the levels of happiness. These results support that the rates of participation in the five types of social activities and their effects on the levels of happiness were different by age groups. The implication of this study was to report that needs and the interests of older individuals are different by age groups based on empirical evidences.

• Korean journal of applied entomology
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• v.61 no.4
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• pp.577-590
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• 2022

Ostrinia scapulalis is one of important pests in leguminous crops, especially red bean. In order to understand the biological characteristics of the insect, we investigated the effects of temperature on development of each life stage, adult longevity and fecundity of O. scapulalis at eleven constant temperatures of 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, and 36℃. Eggs and larvae successfully developed next life stage at most temperature subjected except 7, 10 and 13℃. The developmental period of egg, larva and pupa decreased as temperature increased. Lower and higher threshold temperature (TL and TH) were calculated by the Lobry-Rosso-Flandrois (LRF) and Sharpe-Schoolfield-Ikemoto (SSI) models. The lower developmental threshold (LDT) and thermal constant (K) from egg hatching to adult emergence of O. scapulalis were estimated by linear regression as 13.5℃ and 384.5DD, respectively. TL and TH from egg hatching to adult emergence using SSI model were 19.4℃ and 39.8℃. Thermal windows, i.e., the range in temperature between the minimum and maximum rate of development, of O. scapulalis was 20.4℃. Adults produced viable eggs at the temperature range between 16℃ and 34℃, and showed a maximum number, ca. 416 offsprings, at 25℃. Adult models including aging rate, age-specific survival rate, age-specific cumulative oviposition, and temperature-dependent fecundity were constructed, using the temperature-dependent adult traits. Temperature-dependent development models and adult oviposition models will be useful components to understand the population dynamics of O. scapulalis and will be expected using a basic data for establishing the strategy of integrated pest management in leguminous crops.

• Korean Journal of Social Welfare Studies
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• v.42 no.1
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• pp.399-432
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• 2011

The purpose of this study is to understand the adjustment process of families adopting an older child, and to generate a substantial theory. To achieve this purpose, we conducted in-depth interviews with mothers adopting an older child and analyzed data with qualitative analysis approach. From the analysis, theoretical model has been made, and the model includes the adoptive families' diverse experiences, barriers to adjustment as well as resources and strategies that they mobilized and used for smooth adjustment. Their experiences in the process of adjustment consisted of five phases: unfamiliar meeting, shock, fighting alone without support, control, and stability. Barriers to adjustment process were composed of adoptees' problem behaviors, loss of time, lack of preparation, lack of experiences, repetition of the vicious circle, withstanding alone, improper resources, lack of support, and being criticized. Resources and strategies that families adopting an older child utilized were individual resources and ability such as rearing experiences, intellectual ability, willingness, belief, and perspective change; family system such as spouse and other offsprings; informal support system such as extended families, relatives, friends, neighbors, and other families adopting an older child; formal resources such as adoption workers and helping professionals. From our results, we suggested policy and practice guidelines to help adjustment experiences for families adopting an older child.