• Journal of Bio-Environment Control
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• v.27 no.1
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• pp.86-93
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• 2018

This present study was conducted to develop the domestic cultivation kit using water celery(Oenanthe Stolonifera DC.) seed. As the result of germination rates in 3 type inbred lines, the IT 232354 had the highest initial germination rate and final germination rate, and was selected as the inbred line to be used in the cultivation kit. The development of the domestic cultivation kit was carried out using the IT 232354 seeds. It was possible to cultivate up to 3 times harvest using the same root in this cultivation kit, though the growth decreased rapidly in the $4^{th}$ cultivation. As a result of investigating the effects of the type of nutrient solution on growth of water celery, the overall growth was the lowest in the nutrient solution for Oenanthe Stolonifera DC.(N.S.D.). The shoot growth was similar to that of nutrient solution for leaves and stem vegetables (N.S.L.S.) and amino acid fermentation by-product liquid (A.F.B.L.), however in the root growth, the N.S.L.S. was more effective than A.F.B.L. When it was harvested 4 times consecutively after 1 time of planting, the last survival ratio of A.F.B.L. was 100% while their ratios were 96.4% and 49.8% each for N.S.L.S. and N.S.D. For the growth by cultivation kit type, the hole type cultivation kit increased slightly compared to the 3 hole type cultivation kit in the $1^{st}$ and $2^{nd}$ harvest, but there was no difference in the $3^{rd}$ and $4^{th}$ harvest. Total yield of one cultivation kits showed the 3 hole type cultivation kit is much higher than the 2 hole type cultivation kit. According to the results of this experiment, it is possible to harvest three times by planting one times if it was cultivated using N.S.L.S. and A.F.B.L. in the 3 hole type cultivation kit.

• Applied Biological Chemistry
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• v.39 no.6
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• pp.494-500
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• 1996

Uptake of hen metal ions by water dropwort (Oenanthe stolonifera DC.) and its cadmium-binding protein were studied to probe for good method to remove heavy metal contaminants from environments. The plant was cultured in the culture medium (pH 7.0) containing the various concentrations of $Cd^{2+}$, $Cr^{3+}$ or $Pb^{2+}$, for 3 and 7 days. The residual heavy metals deposited in roots linearly increased as the metal ions concentration increased up to 17 ppm for $Cd^{2+}$, 20 ppm for $Cr^{3+}$ and 50 ppm for $Pb^{2+}$. Above these concentrations, the plant growth was inhibited and the uptake rates of the metal ions decreased. The heavy metals absorbed by the plant were mostly deposited in roots. In particular, the residual concentration of lead in roots was about four times higher than those of cadmium and chromium. When cultured in the medium containing 20 ppm of each metal ion, 80% of cadmium, 90% of cromium and 96% of lead were deposited in roots out of the total residual metal ions in the plant. These values correspond to 6.1 mg of cadmium, 5.2 mg of chromium and 23.6 mg of lead per one gram of roots tissue on a dry weight basis. A cadmium-binding protein was partially purified by extraction, gel filtration and DEAE-Cellulose chromatography from water dropworts that was grown in the medium containing 20 ppm $Cd^{2+}$. The purified protein was a single band on SDS- and non-denaturing- polyacrylamide gel electrophoresis. Its molecular mass was estimated to be ca. 5,000 dalton by gel filteration. Analysis of amino acid composition of the protein indicated that it had a typical amino acid composition of heavy metal-binding protein in that it contained 27% of acidic amino acids and 9.9% of cysteine. However, it is likely that the protein is a new plant metal-binding protein, since its amino acid composition is somewhat different from those of phytochelatins that have been known so far.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.423-431
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• 2022

Due to increase of interest in 'carbon neutrality', attempts at agricultural use of CO₂ are increasing. In this study, we used the dry-ice made by CO₂ as by-product in thermoelectric power plant on CO₂ fertilization for production of leafy vegetable in greenhouses. The dry-ice was supplied on three leafy vegetable farms (Allium tuberosum Rottl. ex Spreng, Aster scaber, and Oenanthe stolonifera DC.) located in Hadong, Gyeongsangnamdo. Two greenhouses were used in each leaf vegetable crops, one greenhouse used as the control (non-treatment), other greenhouse used as supplied CO₂. For CO₂ fertilization, a gas sublimated from dry ice was supplied to the greenhouse using a specially designed prototype supply machine. A. tuberosum greenhouse has no difference of CO₂ concentration between the control, and CO₂ fertilization and shown high CO₂ concentration both greenhouses. However, the CO₂ concentrations in A. scaber and O. stolonifera greenhouses were increased in CO₂ fertilization treatment. The growth of A. scaber and O. stolonifera were increased in CO₂ fertilization, and the yield also increased to 36% and 25% than the control, respectively. As a result of economic analysis, the A. scaber has increase of income rate, however A. tuberosum and O. stolonifera has decreased income rate. Thus, the use of the dry-ice made by CO₂ as by-product in thermoelectric power plant has possibility to increase productivity of the leafy vegetable in greenhouse and have agricultural use value.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2001.11a
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• pp.174-175
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• 2001

미나리 묘를 대량으로 생산하는데 있어서 체세포배를 이용하는 방법이 매우 유리하다. 체세포배는 계절에 영향을 받지 않고 좁은 공간에서 한꺼번에 많은 량의 묘를 생산할 수 있다. 또한 생물반응기 등을 이용하여 대량 생산할 수 있으면 실생묘를 대체할 수 있을 것으로 생각된다. 배양기 내의 공기 환경의 영향은 삼각플라스크를 이용한 소규모 배양에서는 문제가 되지 않았지만 배양기가 커짐에 따라 공기 환경의 변화도 커져 체세포배의 발생과 발아에 영향을 미친다. 체세포배를 배양하는 기간은 배발생과 발아로 나누어, 세포에서 배가 발생하는 처음 2주 동안에는 밀폐 환경, 즉 환기가 거의 되지 않는 환경이 유리하지만 이후의 발아 동안에는 산소를 많이 요구한다. 삼각플라스크를 이용한 소규모 배양에서는 aluminum foil을 플라스크의 뚜껑으로 하여, 배지를 교체할 때를 제외하고는 배발생과 발아가 진행되는 전 배양 기간 동안 이를 유지한다. 배가 발생하는 초기 2주의 경우는 이 밀폐 환경이 발아를 촉진시킨다. 그러나 배가 발아하여 정상 식물체로 발달하는데는 유리하지 않다. 체세포배는 발아할 때 산소를 많이 필요로 하며 에틸렌이 많이 축적되면 발아율이 낮아지며, 적절한 공기 환경이 주어지면 체세포배 발아가 동조화되어 균일한 묘를 얻을 수 있고 수확시기도 예측할 수 있다. 본 실험에서는 배가 발아하는 기간 동안 플라스크 내의 공기 환경을 다르게 하기 위해 플라스크를 막는 뚜껑의 소재를 달리하여 배양한 후 발아율을 측정하였다. 또한 가장 효율적인 환기량을 구명하기 위하여 인위적으로 플라스크에 공기를 넣어 강제 환기시키는 실험을 수행하였다. 미나리 cell은 처음 8일까지는 생장을 하지 않다가 이후 급속히 생장을 시작하여 35일 정도까지는 생장을 하다가 다시 생장이 둔화되었다. 밀폐시킨 삼각플라스크에서 자라는 Cell은 상태도 좋지 않고 전반적인 증식량도 적었다. Cell은 환기정도에 민감한 것으로 판단되며 삼각플라스크에서 약 35일 정도의 생장 주기를 가지는 것으로 사료된다. 배양 3주까지는 플라스틱 뚜껑으로 밀폐시킨 bottle에서 가장 많은 체세포배를 얻었다. Air filter를 달아 2일 마다 신선한 공기를 넣어 주었을 때는 배의 발달이 많이 늦어져 배양 3주째에 다른 처리보다 배의 수가 훨씬 적었다. 체세포배가 발달하는 동안에는 산소를 많이 요구하지 않으나 성숙하는 동안에는 산소를 많이 요구하는 것으로 생각된다.

• Journal of the East Asian Society of Dietary Life
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• v.21 no.3
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• pp.338-344
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• 2011

In this study, muffins were produced using dropwort powder and to the quality characteristics such as volume, height and moisture content The results showed that the height of the muffin was 5 cm and there were no significant differences across samples (p<0.05). The volume of the muffin increased as the amount of dropwort powder increased. With respect to the moisture content, a comparison group showed a moisture content of 26%, and the 3%-added and the 6%-added groups did not show any significant difference. The 9%-added group, however, did show a significant difference (p<0.05). The results of texture measurements showed that hardness increased as the amount of added dropwort powder increased. There were also significant differences in adhesiveness and springing across samples (p<0.05). With respect to cohesiveness, gumminess and chewiness, these characteristics tended to increase as the amount of added dropwort powder increased, and there were significant differences across samples (p<0.05). Sensory test results of added showed that the 3%-added group scored the highest for color, flavor and taste. For texture, the 6%-added group showed a value of 6.2, which was the highest texture. In terms of the overall acceptability, the 3% dropwort powder group showed a score of 7.2, which was the highest acceptability. These results suggest that the 3%-added group was the most desirable and appropriate.

• Journal of Bio-Environment Control
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• v.26 no.2
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• pp.146-150
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• 2017

This research investigated the effect of photo/dark period and relative humidity during dark period on the growth and quality of water dropwort in a closed-type plant factory system. At 30 days after planting, the shoot fresh weight of water dropwort under relative humidity of 60/90%(light/dark) treatment significantly higher than that under relative humidity of 60/60% treatment. The shoot fresh weight of water dropwort increased by extending light period under relative humidity of 60/60% treatment, but 16/8h photo/dark period showed the best shoot fresh weight, followed by 20/4h and 22/2h under relative humidity of 60/90% treatment. In the relative humidity of 60/90% treatment, the tipburn occurrence was reduced under 16/8h photo/dark period condition as 1.4%, whereas it was significantly increased under 20/4h and 22/2h of relatively long light time duration as 15.5% and 30.3%, respectively. In the relative humidity of 60/60% treatment, the tipburn occurrence was 15.5% under 16/8h photo/dark period condition and those under 20/4h and 22/2h photo/dark period condition were higher than 25%. The stem hardness of water dropwort was lowest in relative humidity of 60/90% and 16/8h photo/dark period treatment. The mineral contents of leaves were decreased by extending light period, but the contents of Ca were not different significantly among the treatments except the 60/60% and 22/2h treatment.

• Korean Journal of Medicinal Crop Science
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• v.7 no.2
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• pp.107-114
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• 1999

It has long been recognized that dropwort contains specific funtional subtances for protecting human liver and preventing and curing its diseases, and thus it has been widely utilized in traditional folk remedy. In the present study. fresh of fermented extract of dropwort shoots grown on dryland and fresh extract of those grown on flooded fie1d were fed to the rats suffering from acute, subacute or chronical toxication induced by alcohol administration, and their affects were investigated. Administration of alcohol to rats and mice for 2 days at 5ml of 30% EtOH/kg/day raised total cholesterol and total glyceride which were, however, great1y supressed when alcohol was administered to the laboratory animals previously fed on fresh or fermented extract of dryland dropwort, or fresh extract of flooded field-grown dropwort for 20 days, without significant differences among the extracts. The levels of alanine aminotransferase and aspartate aminotransferase which were raised by alcohol adminstration were also lowered by feeding dropwort extract, among which that of fermented dryland-grown one was more effective than the other two. Chronic alcohol toxication was induced to rats by administering 10% alcohol for 10 months and fermented dropwort extract or tap water was fed to the rats for 5 days. The rats fed on fermented dropwort extract were lower in total cholesterol by 40% and in tota1 glyceride by 60% than the control. Alanine aminotransferase and aspartate aminotransferase in the rats fed on fermented dropwort extract were decreased by 87.2% and 91.7%, respectively, compared to the control, and the rats recovered almost to normal. Activity of alkaline phosphatase, catalase, superoxide dismutase or glutathione peroxidase changed greatly by alcohol administration in the rats suffering from chronic as well as acute toxication. The extract of fermented dry land dropwort significantly lowed the activity of those enzymes, especially, alkaline phosphatase and superoxide dismutase. The present results suggesting the possible medicinal effect of fermented dropwort extract to liver diseases.