• Applied Microscopy
• /
• 제31권4호
• /
• pp.333-345
• /
• 2001

두족류 3종(Octopus minor, Octopus ocellatus, Todarodes pacificus)의 성숙정자를 전자현미경을 통해 관찰한 결과 다음과 같았다. 팔완류인 서해박지와 주꾸미의 성숙정자 전체 길이는 각각 $390{\mu}m$와 $125\sim130{\mu}m$정도였고, 십완류인 살오징어의 길이는 $35{\mu}m$정도로 매우 짧았다. 서해낙지는 나선형의 첨체와 약간 굽은 바나나 모양의 머리를 소지하고 있었으며, 주꾸미는 꼬인 첨체와 막대 모양의 긴 머리를 가지고 있었다. 이들 침체 내강에는 규칙적 구조(periodic structure)인 많은 가로무늬가 관찰되었고, 머리의 내강에는 치밀전 (dense plug)을 형성하였다. 그러나 살오징어의 첨체는 전자밀도가 낮은 둥근 모자 모양이 였으며, 머리는 길쪽한 장타원형을 나타내었다. 특히 첨체 하단부에는 2개의 작은 강(cavity)이 관찰되었고, 이들 내강에는 전자밀도가 높은 물질들(juxtanuclear acrosomal materials)로 채워져 있었다. 3종의 성숙정자 중편은 서해낙지와 주꾸미에서 미토콘드리아가 mitochondrial sleeve를 형성하였지만, 두 종간 미토콘드리아의 수는 각각 $11\sim12$개와 $8\sim9$개로 관찰되어 수의 차이를 보였다. 반면, 살오징어는 미토콘드리아가 축사와 분리되어 mitochondrial spur를 형성하였고, 이들 spur내에는 $10\sim13$개의 미토콘드리아와 전자밀도가 높은 물질들이 밀집되어 있었다. 서해낙지, 주꾸미, 그리고 살오징어의 축사는 공히 9+2구조에 9개의 금은섬유가 둘러싸고 있었고, 살오징어의 축삭내에서만 작은 입자형의 글리코겐이 관찰되었다. 금은섬유들은 서해낙지와 살오징어에서는 꼬리의 주편까지만 관찰되었으나, 주꾸미에서는 단편에서도 관찰되었다.

• The Korean Journal of Legal Medicine
• /
• 제42권4호
• /
• pp.168-171
• /
• 2018

In Korea, small octopus (Octopus minor) and webfoot octopus (Octopus ocellatus) are food items and fatal laryngeal choking due to ingestion of live octopus is not uncommon. We recently encountered two autopsy cases of accidental choking on small octopus and webfoot octopus. Case 1 involved a 58-year-old fisherman who ingested two live webfoot octopuses in his fishing boat and collapsed. He was immediately taken to the hospital but died. During autopsy, one of the webfoot octopuses was found between his pharynx and esophagus; it was obstructing the epiglottis and upper esophagus. His blood alcohol concentration was 0.140%. Case 2 involved a 55-year-old man who ingested an intact body part of a small octopus and was found dead in his house. He had a history of cerebral infarction and angina pectoris. During autopsy, an intact body part of the small octopus was found to be lodged in the laryngeal inlet.

• 한국패류학회지
• /
• 제32권2호
• /
• pp.133-139
• /
• 2016

The incubation time required for hatching of O. ocellatus were investigated through the processes of egg and embryonic developments by the dissecting microscopic and visual observations. And differences in ecological characteristics of the plankton mode of life or the benthic mode of life according to total numbers of the suckers on each short arm of the hatched juvenile larvae of O. ocellatus were studied by comparisons with other octopodidae species. Compared with the recent a few results reported by other researchers associated with the incubation time required for hatching by female adult mother of O. minor (73-90 days after spawning at $20.9-21.5^{\circ}C$ ranges), in this study, the incubation time required for hatching by female adult mother of O. ocellatus was 56-57 days after spawning at $11.0-20.4^{\circ}C$. Therefore, the incubation time required for hatching by female adult mother varied with Octopodidae species. In this studies, each ovarian egg laid by a female was connected to an egg string attaching to the surface of the wall or bottom of vacunt shell of Rapana venosa. Egg and embryonic developments of this species were studied in the indoor aquaria, in the specific gravity ranging 1.024-1.025. the hatched juvenile of O. ocellatus is 10.3 mm in the mean total length and 4.5 mm in mantle length, and each of its short arms has 18-20 suckers. The just hatched juvenile larvae of O. ocellatus enter the benthic mode of life (benthic larval stage) after hatching. In particular, regarding differences in ecological characteristics of the mode of life according to total numbers of the suckers, O. vulgaris may not need to have many suckers because they enter the planktonic mode of life after hatching, however O. ocellatus may need to have many suckers, because they should adapt to the benthic mode of life. And also the just hatched juvenile larvae of O. minor (bearing many suckers more than O. ocellatus) enter the benthic mode of life (benthic larval stage) after hatching. Therefore, the total number of the suckers on each short arm of the hatched juvenile larvae can be used for determining whether an octopus species has planktonic larval stages or benthic larval stage (benthic mode of life). In particular, The intracohort cannibalism phenomena appeared at the hatched juvenile larval stage because the larval stage of O. ocellatus and O. minor enter into the benthic larval stage in the early stage, unlike entering into the plaktonic larval stage in other Octopus species such as O. vulgaris: at this time, the early hatched larvae fed the late hatched larvae (they are the same species and almost same ages). Therefore, the intracohort cannibalism pheneomena occur in the just hatched juvenile stage of only O. ocellatus and O. minor.

• 한국전자현미경학회:학술대회논문집
• /
• 한국현미경학회 2001년도 제32차 추계학술대회
• /
• pp.51-53
• /
• 2001

• 한국패류학회지
• /
• 제20권1호
• /
• pp.55-63
• /
• 2004

Gonadosomatic index, condition index and reproductive cycle with the gonadal development of the female Octopus ocellatus were investigated by histological observations and morphometric data, from January to December, 2000. And changes in biochemical components of the ovary and the trunk tissues including the digestive organ associated with gonadal development were studied by biochemical analysis from January to October, 2001. The specimens were collected at the coastal waters of Buan, Jeollabuk-do, Korea, from January 2000 to October 2001. O. ocellatus is a dioecious organism. The gonad of O. ocellatus locates medially in posterior region of the body. Morphology of the ovary shows round and oval in shape, the average diameter and external colour of ripe ovary was 32 mm and semitransparent light brown in colour. As the ovary was getting mature, transparent elongated eggs covered with chorion were present in the ovarian cavity. Monthly changes in the gonadosomatic index (GSI) showed a similar pattern with those of the condition index. The GSI and condition index began to increase in March and reached the maximum in April. And then, their values decreased from May and reached the minimum in September. Reproductive cycle of O. ocellatus can be categorized into five successive stages: early developing stage (September to December), late developing stage (November to March), ripe stage (March to May), partially spawned stage (April to June), and degenerative/resting stage (June to October). Follicle cells attached to an oocyte were involved in vitellogenesis in the cytoplasm of the vitellogeneic oocyte and formation of chorion (secondary egg membrane) of the ovarian eggs. Spawning occurred between April and June. The spawning period was once a year and the peak took place between May and June. This species belongs to semelparity. According to changes in biochemical contents of the ovary and the digestive organ, monthly variations of moisture, total protein, total lipid and glycogen contents (%) in the ovary showed a negative correlationship with those of the trunk tissues including the digestive organ. Accordingly, it is assumed that the ovary only may be received nutrient supply (total lipid content) for gonadal development from the trunk tissues including the digestive organ (r = -0.55, p < 0.05).

• 한국패류학회지
• /
• 제31권2호
• /
• pp.113-121
• /
• 2015

Gametogenesis, mating behaviour and spawning of Octopus ocellatus were investigated by histological study. This species is dioecious, and showed a protandry phenomenon. Ooogenesis (in females) and spermatogenesis (in males) can be classified into 3 stages, respectively. O. ocellatus copulates in one of two ways: a male may leap upon a female, mounting her mantle, or a male may sit near the female and extend the hectocotylized third right arm toward her. Spawning occurred between April and June in females, and between March and May in males of O. ocellatus. The spawning period was once a year and the peak took place between May and June. A number of flatened follicle cells, which were attached to an oocyte, were involved in vitellogenesis in the cytoplasm of the vitellogenic oocyte (maturing oocyte), and formation of chorion membrane (secondary egg membrane) of the ovarian eggs. Fecundity per female closely related to GSI was 294-660 eggs (average, 429 eggs). The diameters of the ovarian eggs surrounded by chorion membrane were approximately in the range of 10.10-2.50 mm. Each ovarian egg laid by a female was connected to an egg string. Each egg string was 1-5.5 cm (average 3.6 cm). The total number of eggs laid by a female of this species ranged 218-314, the egg sizes were independent to the size of female adult. this species has a life mode showing some special reproductive characteristics of an annual semelparity as shown in Octopodidae species because we have never seen a female spawning a second time.

• 수산해양기술연구
• /
• 제26권3호
• /
• pp.237-243
• /
• 1990

본 연구는 수조실험을 통하여 주꾸미, Octopus Ocellatus GRAY의 단지색에 대한 선택성을 조사하고, 전통적인 주꾸미 단지의 재질을 고둥껍질에서 PVC로 대체할 경우 그 가능성을 규명하기 위하여 PVC 시험 단지를 제작하여 1989년부터 1990년까지 해상실험을 실시하여 어획율을 조사하였다. 실험에서 얻은 결과를 요약하면 다음과 같다. 1. 수중실험에서 적색, 녹색, 황색, 백색, 회색중에서 주꾸미가 선호하는 색은 회색, 적색, 녹색, 황색, 백색순으로 나타났다. 2. 해상실험에서 주꾸미의 선호색은 회색, 홍색, 녹색순으로 나타났고, 이것은 수조실험의 결과와 일치한다. 3. 어획성능면에서 고둥껍질과 회색 PVC 단지의 차이는 거의 없었다. 4. 직경별 어획율은 40mm PVC 단지가 1.00, 50mm가 0.19로 나타났다.

• 한국어업기술학회:학술대회논문집
• /
• 한국어업기술학회 2002년도 춘계 수산관련학회 공둥학술발표회
• /
• pp.623-624
• /
• 2002

• 한국패류학회:학술대회논문집
• /
• 한국패류학회 2002년도 춘계학술대회
• /
• pp.9-10
• /
• 2002

• 한국패류학회지
• /
• 제19권1호
• /
• pp.71-79
• /
• 2003

두족류는 흡반에 의한 흡착력이 강하여 방류시 분리 포획에 어려움이 있는 어린 주꾸미의 마취 소요시간에 따른 효과면에서 200 ppm 이하의 농도에서 염산리도카인이 MS-222보다 1.6-4.5배정도 마취가 빨랐고 300-500 ppm의 농도에서는 5.10-6.0배 빨랐다. 노출시간에 따른 마취율과 회복률에 염산리도카인이 MS-222보다 저농도에서 짧은 시간 내 마취되고 고농도에서 빠른 시간 내에 회복되었다. 1차 마취 후 경과시간별 염산리도카인의 재마취에서 농도가 낮고 경과시간이 길수록 마취개시 및 종료시간이 늦어지고 농도가 높고 경과시간이 짧을수록 마취개시 및 종료시간이 빨라졌다. 재마취 회복 또한 저농도에서 경과시간이 길수록 빠르고 고농도에서 경과시간이 짧을 수록 회복이 늦어졌다. 주꾸미의 경우 MS-222보다는 염산리도카인이 마취에 좋았으며 작업을 고려한 사용농도 및 한계시간은 100 ppm 농도에 15분 이내가 적당하였다.