• Journal of Korean Society for Atmospheric Environment
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• v.8 no.3
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• pp.198-203
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• 1992

Theoretical predictions of the atmospheric equilibrium involving $HNO_3, NH_3 and NH_4NO_3$ were compared with atmospheric measurements of particulate nitrate$(NO_3^-)$, $HNO_3$ and $NH_3$ concentration by triple filter pack sampler and Andersen air sampler in Seoul from 8th to 11th Oct 1991. The measured $HNO_3-NH_3$ concentration product K was higher than equilibrium costant Kc calculated from thermodynamic data of $HN_4NO_{3(s)} -HNO_{3(g)} -NH_{3(g)}$. The cause of K is greater than Kc was the result of air pollution, partcicularly anthropogenic $NH_3$.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.12
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• pp.2021-2030
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• 2020

Objective: Quantitative polymerase chain reaction (qPCR) has been extensively used in the field of mesenchymal stem cell (MSC) research to elucidate their characteristics and clinical potential by normalization of target genes against reference genes (RGs), which are believed to be stably expressed irrespective of various experimental conditions. However, the expression of RGs is also variable depending on the experimental conditions, which may lead to false or contradictory conclusions upon normalization. Due to the current lack of information for a clear list of stable RGs in bovine MSCs, we conducted this study to identify suitable RGs in bovine MSCs. Methods: The cycle threshold values of ten traditionally used RGs (18S ribosomal RNA [18S], beta-2-microglobulin [B2M], H2A histone family, member Z [H2A], peptidylprolyl isomerase A [PPIA], ribosomal protein 4 [RPL4], succinate dehydrogenase complex, subunit A [SDHA], beta actin [ACTB], glyceraldehyde-3-phosphate dehydrogenase [GAPDH], TATA box binding protein [TBP], and hypoxanthine phosphoribosyltrasnfrase1 [HPRT1]) in bovine bone marrow-derived MSCs (bBMMSCs) were validated for their stabilities using three types of RG evaluation algorithms (geNorm, Normfinder, and Bestkeeper). The effect of validated RGs was then verified by normalization of lineage-specific genes (fatty acid binding protein 4 [FABP4] and osteonectin [ON]) expressions during differentiations of bBMMSCs or POU class 5 homeobox 1 (OCT4) expression between bBMMSCs and dermal skins. Results: Based on the results obtained for the three most stable RGs from geNorm (TBP, RPL4, and H2A), Normfinder (TBP, RPL4, and SDHA), and Bestkeeper (TBP, RPL4, and SDHA), it was comprehensively determined that TBP and RPL4 were the most stable RGs in bBMMSCs. However, traditional RGs were suggested to be the least stable (18S) or moderately stable (GAPDH and ACTB) in bBMMSCs. Normalization of FABP4 or ON against TBP, RPL4, and 18S presented significant differences during differentiation of bBMMSCs. However, although significantly low expression of OCT4 was detected in dermal skins compared to that in bBMMSCs when TBP and RPL4 were used in normalization, normalization against 18S exhibited no significance. Conclusion: This study proposes that TBP and RPL4 were suitable as stable RGs for qPCR study in bovine MSCs.

• Quality Improvement in Health Care
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• v.5 no.2
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• pp.224-237
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• 1998

Background : This is an investigative study to analyse the importance of works perceived by Quality Improvement(QI) Coordinators and to evaluate their current work performances using the questionnaires developed by the investigator. Methods : The data were collected from 37 subjects over two weeks period from Oct. 1 to Oct. 17, 1998 and analysed by the descriptive statistics of SPSS program. The items of questionnaire are consisted of 13 work domains including 73 activities based on Quality Management(QM) Coordinator's job description of National Association for Healthcare Quality:data collection & analysis, communication, monitoring, evaluation, accreditation, tool development, policy development, program development, self development, education & trainning, system design, planning, and consultation & support. Results : 1) Of the performances in 13 work domains, the frequencies of the work performed were accreditation(89%), planning(88%), communication(83%), data collection & analysis(82%), monitoring(76%), policy development(72%), consultation & support (71%), education & trainning(70%), self development(68%), evaluation(63%), tool development(61%), program development(44%) and system design(43%) in order. 2) For the importances (1=not important, 5=very important), the policy development(4.46) scored highest then monitoring(4.42), planning(4.41), education & trainning(4.38), communication(4.35), evaluation(4.34) tool development (4.30), data collection & analysis(4.29), program development(4.22), consultation & support(4.22), accreditation(4.15), self development(4.05) and system design(3.98) in order. 3) There was a difference between the work performance and the perceived importance. The results showed the low performances in policy development, monitoring, education & trainning and evaluation which ranked high by the perceived importance and the high performances in accreditation, data collection & analysis, self development, communication and consultation & support which ranked middle to low by the perceived importance. 4) The reasons for low performances of QI Coordinators were the lack of clear assignment for the responsibility and allowed authority for work to QI Coordinators(30.8%), insufficient member of QI Coordinators(13.9%), lack of hospital director's interest(11.5%), low motivation of QI Coordinators (10.6%) and insufficient knowledge & experience of QI Coordinators (8.8%). Conclusion : Most works were perceived important by QI Coordinators, but there was a difference in the work performance. The works performed over 70% were related with accreditation, data collection & analysis, communication, planning and monitoring, on the other hand under 50% in performances were related with system design, program development, tool development and evaluation.

• Journal of Preventive Medicine and Public Health
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• v.18 no.1
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• pp.59-71
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• 1985

During a period from February 1st to November 30th in 1983, measurements were made twice a month on dustfalls and concentrations of various cations ana anions in their filtrates in an urban (Heuksuk-dong, Seoul) and in a rural (Ansung) districts. Standard British Deposit Gauge method was applied for collection of dustfalls and ion chromatographic method using Ion Chromatograph 10 (Dionex, U.S.A.) for determination of cation and anion concentrations. The results obtained were as follows: 1. Annual mean values of dustfalls were $8.30{\pm}5.09tons/km^2/month$ in an urban and $6.20{\pm}0.82tons/km^2/month$ in a rural districts. And annual mean values of pH of filtrates of the collected samples were $5.3{\pm}0.76$ in an urban and $6.0{\pm}0.82$ in a rural districts. 2. Annual mean concentrations of common cations and anions in their filtrates were as follows: urban rural (unit: ppm) $$Na^{+}\;1.7{\pm}1.30\;1.9{\pm}1.86$$ $$NH^{+}_{4}\;2.4{\pm}1.60\;1.3{\pm}1.59$$ $$K^{+}\;0.7{\pm}0.47\;1.5{\pm}3.79$$ $$Ca^{++}\;5.3{\pm}0.95\;3.4{\pm}2.58$$ $$Mg^{++}\;0.7{\pm}0.89\;0.5{\pm}0.83$$ $$Cl^{-}\;2.0{\pm}1.31\;4.0{\pm}2.35$$ $$NO^{-}_{3}\;4.0{\pm}2.35\;2.7{\pm}1.31$$ $$So^{--}_{4}\;16.9{\pm}17.40\;6.8{\pm}8.00$$ Some of anions such as $Br^{-},\;{PO_4}^{-3}\;and\;{NO_2}^{-}$ were scarecely detected and $F^{-}$ was measured at very low concentrations ranging from 0.1 to 0.5 ppm in Feb., Oct. and Nov. in an urban and in Feb. in a rural districts. 3. By two-way analysis of variance with 4 replications for dustfalls, pH and concentrations of various ion by district and month of a year, statistically significant differences were noted in dustfalls (p<0.01), pH (p<0.01) and concentrations of various ion (p<0.01) by month of a year, and in dustfalls (p<0.05) and concentrations of various ion such as ${NH_4}^{+},\;Ca^{++},\;{NO_{3}}^{-}\;and\;{SO_4}^{--}$ (p<0.01) by district. 4. There were statistically significant differences pH in Spring (Feb., Mar and Apr.) (p<0.01) and in ${SO_4}^{--}$ in Autumn (Aug., Sept., Oct. and Nov.) (p<0.05) between the two districts.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.4
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• pp.359-365
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• 1994

A oat (Avena sativa) cultivar, 'Guiri 16', was seeded on Oct. 9, Oct. 24, Nov. 9, Nov. 24, Dec. 9 of 1991 and 1992 to determine usefulness of growing degree days (GDD) for predicting growth duration and the optimum seeding date of oats for grains in Cheju province. The later the seeding, the greater the number of days to emergence but the fewer the number of days to heading and maturity. As seeding was delayed, accumulated GDD from seeding to emergence generally tended to decrease but was less subjective to a constant downward tend over seeding date than the number of days. Accumulated GDD from emergence ot heading decreased with delaying seeding and accumulated GDD from heading to maturity decreased as seeding was delayed up to Nov. 24. As seeding was delayed from Oct. 9 to Dec. 9, to Dec. 9, leaf area index at heading decreased from 7.7 to 5.1 and dry matter yield at maturity from 1920 to 823 kg /10a in 1992-1993, and culm length 120 to 89cm on an average of 1991-1992 and 1992-1993. While the number of grains per panicle and test weight were not affected by seeding date in 1991-1992 and 1992-1993, the number of panicles per m$^2$ and grain yield were decreased when oats were seeded earlier or later than Nov. 9. 1000 grain weight was not affected by seeding date in 1991-1992 but greatest at Nov. 9 seeding in 1992-1993. The results indicate that optimum seeding date of oats in Cheju province would be early November. November.

• Bulletin of the Korean Chemical Society
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• v.27 no.12
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• pp.2051-2054
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• 2006

The molecular structures of the ground and lowest triplet states of 2,3-diazabicyclo[2.2.1]hept-2-ene (DBH), 2,3-diazabicyclo[2.2.2]oct-2-ene (DBO) and their fused ring derivatives are investigated with an ab initio method and the density functional theory. Unlike the singlet DBH and DBO, the azo skeletal structures of the triplet counterparts are turned out to be quite sensitive to the change of the electronic structure of the fused ring. The B3LYP C-N=N-C dihedral angles of the triplet DBH and DBO are estimated to be about 28.0 and $40.4{^{\circ}}$, respectively. The B3LYP singlet-triplet energy gaps for DBH and DBO are predicted to be 58.4 and 48.4 kcal/mol, respectively. The triplet state energy can be lowered drastically by the presence of the remote $\Pi-\Pi$ interaction as in the case of 1bb'.

• Korean Journal of Environmental Agriculture
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• v.23 no.4
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• pp.245-250
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• 2004

An analytical method was developed to determine monocrotophos residues in apple, citrus, and soil using high-performance liquid chromatography (HPLC) with ultraviolet absorption detection. Monocrotophos was extracted with acetone from apple, citrus and moist soil samples. The extract was concentrated, added with saline water, and subjected to n-hexane washing to remove nonpolar co-extractives. Dichloromethane partition was then followed to recover monocrotophos from the aqueous phase. Silica gel column chromatography was employed to further purify the extract prior to HPLC determination. Reverse-phase HPLC using an oct-adecylsilyl column was successfully applied to separate and quantitate the monocrotophos residue in sample extracts at the wavelength of 230 nm. Overall recoveries of monocrotophos from fortified samples averaged $95.3{\pm}2.1%$ (n=6), $970{\pm}0.7%$ (n=6), and $92.8{\pm}4.3%$ (n=12) for apple, citrus, and soil, respectively. The proposed method was quite reproducible and sensitive enough to replace the troublesome gas-liquid chromatographic analysis for monocrotophos residues.

• Development and Reproduction
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• v.11 no.2
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• pp.85-96
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• 2007

Mesenchymal stem cells (MSC) are promising candidates for cell-based therapies. One major obstacle for their clinical use is the unsafety of fetal bovine serum (FBS), which is a crucial part of all media currently used for the culture of MSC. We investigated the effect of human cord serum (HCS) on the growth response, mRNA and protein expressions of human amnion-derived stem cells (HAM). HAM were isolated from the amnion after a Caesarean section and cultured in DMEM supplemented with 10% FBS, 5% HCS or 10% HCS. During culture, their biological characteristics at earlier and later passages were analyzed using RT-PCR and immunocytochemistry. Regardless of serum sources, HAM showed the prominent expression of Oct-4, Rex-1, SCF, FGF-5, BMP-4, nestin, GATA-4, NCAM and HLA ABC genes. The expression profile was observed even at later passages. Similarly, HAM cultured in either FBS or HCS exhibited the distinct protein expression of collagen I, II, III and XII, fibronectin, $\alpha$-smooth muscle actin, vimentin, CK18, CD54, FSP, TRA-1-60, SSEA-3, -4 and HLA ABC. However, desmin expression was only observed in HAM cultured in medium supplemented with FBS and vWF expression was only found in HAM cultured in medium supplemented with HCS. Overall pattern of gene and protein expression of HAM was typical of known adult stem cells such as bone marrow-derived MSC. In conclusion, HCS could be as effective as FBS for the culture of HAM.

• Journal of Life Science
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• v.31 no.10
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• pp.873-884
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• 2021

The purpose of present study is to investigate the role of artesunate (ART) in enhancing anticancer effect of nonsteroidal anti-inflammatory drug (NSAID) on human cancer cells, and we elucidate a possible molecular mechanism of this combination effect. We showed that the combined effect of ART with NSAID such as celecoxib (CCB) or dimethyl-CCB (DMC) in various type of human cancer cells. After ART treatment, the expression of p62, nuclear factor erythroid 2-like 2 (NRF2) and cancer stemness (CS)-related proteins including CD44, CD133, aldehyde dehydrogenase 1 (ALDH1), octamer-binding transcription factor 4 (Oct4), mutated p53 (mutp53) and c-Myc was down-regulated. ART induced autophagy as reduction of the autophagy receptor p62, which was associated with up-regulation of activating transcription factor 4 (ATF4) and C/EBP homologous protein (CHOP), and simultaneous down-regulation of NRF2 and CS-related proteins was occurred in the human cancer cells. These results indicate a possibility that ART activates autophagy through ATF4-CHOP cascade leading to down-regulation of CS-related proteins and subsequently eradicated cancer stem cells. In addition, co-treatment with ART and imatinib was more effective than either drug alone on growth inhibition and apoptosis induction of cancer cells. In conclusion, induction of autophagy-dependent cell death by ART might play a critical role in mediating the synergistic effect of drug combination (ART/NSAID and ART/imatinib). Therefore, ART could be a promising candidate as a chemosensitizer to enhance the anticancer effects of NSAID and imatinib.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.2
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• pp.117-122
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• 2013

This experiment was conducted to evaluate agronomic characteristics, forage quality, and dry matter (DM) yield of hairy vetch 'Cold green', common vetch 'Maxivesa', 'Wild common', 'Rasina', 'Morava', and 'Blanchefleur', at Seongju in Kyeongbuk from 2010 to 2012. The experiment was arranged in randomized complete block design with three replications. Vetchs were seeded on the 10th of Oct. 2010 and on the 3rd of Oct. 2011. Vetch plants were harvested on the 4th of May 2011 and on the 10th of May in 2012. 'Cold green' and 'Wild common' exhibited greater cold tolerance than other cultivars. Fifty percent-flowering of 'Cold green' occurred on the 22nd of Apr., whereas that of 'Wild common' occurred on the 26th of Apr. and those of 'Rasina' and 'Blanchefleur' occurred on 28th of Apr. and that of 'Maxivesa' occurred on 17th of May. The DM yield of 'Cold green' was higher (p<0.05) than all other cultivars, whereas the DM yield of 'Wild common' was higher (p<0.05) than those of the remaining 4 cultivars. According to the results obtained from this study, it is suggested 'Wild common' vetch is be recommendable for cultivation where increased DM production is sought.