• Title/Summary/Keyword: Observed quantity

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Evaluation of the Genetic Toxicity of Synthetic Chemicals (XIII) - Single Cell Gel Electrophoresis of Benzoyl Chloride, 2-Propyn-1-ol, and 2-Phenoxyethanol in Chinese Hamster lung Fibroblast -

  • Ryu, Jae-Chun;Kim, Youn-Jung
    • Environmental Mutagens and Carcinogens
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    • v.24 no.2
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    • pp.79-84
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    • 2004
  • Three synthetic chemicals, benzoyl chloride, 2-propyn-l-ol, and 2-phenoxy ethanol were selected for genotoxicity testing, based on production quantity and available genotoxic data. In our previous report, benzoyl chloride induced chromosomal aberrations in Chinese hamster lung (CHL) fibroblast in vitro with and without metabolic activation, while 2-propyn-l-ol and 2-phenoxy ethanol induced only with metabolic activation. To compare the genotoxicity of chromosome aberration assay, the single cell gel electrophoresis (comet) assay subjected using CHL cells. As a result, statistically significant differences of tail moment values of benzoyl chloride, 2-propyn-1-ol, and 2-phenoxy ethanol were observed compared with control values on almost all concentrations with S9 or without S9 metabolic activation system. This results suggest that genotoxic results of the comet assay and the chromosome aberration assay show correlationship of genotoxicity in the CHL fibroblast. In summary, the positive result of chromosome aberration of benzoyl chloride, 2-propyn-l-ol, and 2-phenoxy ethanol was also induced DNA damages in comet assay with same cell line. Consequently, comet assay will be useful and more accurate tool to detect and to confirm the genotoxicity especially DNA damages in CHL fibroblast.

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Effect of Mn Addition on Rolling Contact Fatigue of C-Base Induction Hardened Bearing Steels (C계 유도경화 베어링강의 회전접촉 피로거동에 미치는 Mn 첨가의 영향)

  • Jung, Kyung-Jo;Yoon, Kee-Bong;Choi, Byung-Young
    • Journal of the Korean Society for Heat Treatment
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    • v.8 no.3
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    • pp.205-212
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    • 1995
  • Effect of Mn addition on rolling contact fatigue of C-base induction hardened bearing steels has been investigated to develop inexpensive surface-hardened bearing steels with improved resistance to rolling contact fatigue. Fatigue tests were conducted in elasto-hydrodynamic lubricating conditions at a shaft speed of 5,000rpm, under max. Hertzian stress of $492kg/mm^2$. It was found in the C-Mn steels that effective depth of induction hardened layer and amount of retained austenite were slightly increased in comparison with those of C-base steels. finer interlamellar spacing of pearlite in the C-Mn steels was also observed using TEM. Decomposition of retained austenite during rolling contact fatigue was smaller in quantity in the C-Mn steels than C-base steels. This might be associated with enhanced mechanical stability of retained austenite with addition of Mn. Statistical analysis of fatigue life for C-Mn steels using Weibull distribution indicated that improved resistance to rolling contact fatigue was mainly attributed to transformation induced plasticity and mechanical stability of retained austenite.

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A Study on the Piston Temperatures and Carbon Deposit Formation in LPG Fuelled Engine (LPGdusfy 엔진의 피스톤온도 및 카본디포짓 형성에 관한 연구)

  • 민병순;최재권;박찬준
    • Transactions of the Korean Society of Automotive Engineers
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    • v.6 no.2
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    • pp.100-106
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    • 1998
  • The wide open throttle performance and piston temperatures were measured by the change of fuel : gasoline and liquefied petroleum gas(LPG). Bench test method was developed and experimented to study the effect of temperature on the formation of carbon deposit. The bench test results were confirmed by measuring the piston temperature and observing the deposit production rate at an actual engine running condition. Results show that if the fuel of spark ignition engine is changed from gasoline to LPG, the output power decreases about 10% and the piston temperatures increase about 40~55$^{\circ}C$. In actual engine tests, because of this temperature increase, it was observed that the quantity of carbon deposit in the top ring groove increased in a big degree. Consuquently, it is known that the fing sticking may occur if the gasoline engine was rebuilt to LPG fuelled engine. Therefore, in order to preserve the durability of LPG fuelled engine, it is necessary to lower the piston temperature by hardware modificationor to reduce the carbon deposit by the improvement of engine oil.

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Genetic DNA Marker for A2 mating type in Phytophthora infestans

  • Kim, Kwon-Jong;Lee, Youn-Su
    • Journal of Microbiology
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    • v.40 no.4
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    • pp.254-259
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    • 2002
  • The Phytophthora infestans requires two mating types for sexual reproduction. Amplified fragment length polymorphism (AFLP) was used to specifically detect different mating types of P. infestans. The AFLP primers E+AA (5'-GACTGCGTACCAATTCAA-3') and M+CAA (5'-GATGAGTCCTGAG-TAAC AA-3') detected a fragment that is specific in the A2 mating type of P. infestans. This fragment was cloned and sequenced. Based on the sequence data, PHYB-1 and PHYB-2 primer were designed to detect the A2 mating type of P. infestans. A single 347 bp segment was observed in the A2 mating type of P. infestans, but not in the A1 mating type of P. infestans or other Phytophthora spp. Identification of mating type was performed with phenotype (sexual reproduction) and genotype (CAPs marker) methods. Two factors, the annealing temperature and template DNA quantity, were investigated to determine the optimal conditions. Using mating type-specific primers, a unique band was obtained within annealing temperatures of 57$^{\circ}C$-62$^{\circ}C$ and DNA levels of 10pg-100 ng (data not shown).

A study on the Thermal Characteristics of a Thermal Storage Tank for using Gravels (자갈식 축열조의 축열특성에 관한 연구)

  • Park, Jung-Won;Park, Bong-Kyu;Ahn, Sang-Kyu
    • Solar Energy
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    • v.12 no.1
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    • pp.81-87
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    • 1992
  • The purpose of this study was to investigate the fluid flow characteristics of heat storage in sensible heat storage system for use In cooling and heating of buildings. Heat storage material was gravels and experiments were performed in the condition of constant temperature. The experimental parameters were fluid velocity and size of gravels. The experimental results of the heat storage quantity and the heat storage efficiency by the variation of packing size and fluid velocity were as the follows : The maximum value of the heat storage capacity and heat storage efficiency and the minimum arriving time for maximum heat storage were observed when the packing ratio was 72.5% and the fluid velocity was 0.14m/s.

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Prevalence of Obesity in SchoolChildren from Various Housing Pattern in Seoul (서울지역 10세 아동의 비만이환실태 조사 -주거형태를 중심으로-)

  • 이주연
    • Journal of Nutrition and Health
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    • v.19 no.6
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    • pp.409-419
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    • 1986
  • Prevalence of obesity and dietary consumption of ten-year-old school children in Seoul were investigated. Three hundred forty boys and three hundred forty two girls from families of various housing pattern in Seoul were chosen for this study. Informations of the stduy were obtained from April 22 to June 11, 1985, by means of prepared questinnaires for general environmental factors, anthropometry for height, weigth and triceps skinfold theickness and interview to get 24 -hour food intakes of individuals. All the data collected were analyzed by using SPSS program. Average nutrients intakes of the subjects were satisfactory with respect toquality or quantity . Children from A group tended to consume more nutrients than B or C group. Physical growth of the subjects were similar to the ones for average school children in Seoul, but greater than korean national average. Children from A group were taller and heavier than B group whom were taller and heavier than C group . Prevalence of obesity were 15.7 % and 11.7 % of the subjects according to standards of relative body weight and triceps skinfold thickness. Higher prevalence were observed in boys than in girls and children from A group than B, and B group than C group. so therefore it could be summarized that children from A group consumed more nutrients, had better physical growth, and became obese more frequently than children from B or C.

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Photodynamic Action by Endogenous Non-Chlorophyll Sensitizer As a Cause of Photoinhibition

  • Suh, Hwa-Jin;Kim, Chang-Sook;Jin Jung
    • Journal of Photoscience
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    • v.7 no.3
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    • pp.87-95
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    • 2000
  • As sunlight not always optimized for every terrestrial plant in terms of light quality, quantity and duration, some plants suffer detrimental effects of sunlight exposure under certain conditions. Photoinhibition of photosynthesis is a typical phenomenon representing harmful light effects, commonly observed in many photosynthetic organisms. It is generally accepted that functional, structural loss of photosystem II complex(PSII) is the primary event of photoinhibition. Accumulating data also suggest that singlet oxygen($^1$O$_2$) is the main toxic species directly involved in it. There are two different views on the specific site and mechanism of $^1$O$_2$ production in the photosynthetic membrane. One of them favors the PSII reaction center, where the primary charge pairs recombination occurs as a prerequisite for the generation of $^1$O$_2$, and the other inclines to photosensitized $^1$O$_2$ formation by a substance located outside PSII. This article describes how we, as the advocators of the latter concept, have arrived at the conclusion that $^1$O$_2$ immediately involved in PSII photodamage is largely generated from the Rieske center of the cytochrome b$_{6}$/f complex and diffuses into PSII, attacking the reaction center subunits.s.

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Variation of the Contents of Triterpenoids and Tannins Depending on Growth and Infection in the Leaves of Rubus crataegifolius and Rubus parvifolius

  • Kim, Min-Young;Rhim, Tae-Jin;Lim, Sang-Cheol;Park, Hee-Juhn
    • Natural Product Sciences
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    • v.21 no.1
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    • pp.14-19
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    • 2015
  • Several $19{\alpha}$-hydroxyursane-type triterpenoids and hydrolysable tannins have beneficial effects on human health. Rubus crataegifolius (Rosaceae) has the cleft simple leaf whereas R. parvifolius has pinnate compound leaves. This research was aimed to find the variation in the contents of the triterpenoids and tannins between the infected versus uninfected leaves of R. coreanus and R. parvifolius and between young versus mature leaves. Triterpenoids and tannins were quantitatively analyzed by HPLC. Six triterpenoids including tormentic acid, euscaphic acid, 23-hydroxytormentic acid, coreanoside $F_1$, kaji-ichigoside $F_1$ and niga-ichigoside $F_1$ were used for standard compounds. Gallotannins and ellagitannins were quantitatively evaluated using the indicatives of methyl gallate and ellagic acid. The infected leaves of R. crataegifolius contained higher levels of triterpenoids and tannin than the uninfected leaves; however, lower quantity of total tannin was observed in the mature leaves than in the young leaves. Although the pinnate compound leaves of R. parvifolius exhibited similar tendency of those compositional variation with R. crataegifolius each other, its contents of triterpenoids do not considerably vary. Variation of the contents of triterpenoids and tannins were particularly distinct in R. crataegifolius by growth and infection.

The Supplementary Use of BoneSource® in the Surgical Correction of Craniosynostosis (두개골 조기유합증의 수술적 교정에서 BoneSource®의 보조적 사용)

  • Lim, Jee Hyun;Song, Jin Kyung;Yoo, Gyeol;Byeon, Jun Hee
    • Archives of Plastic Surgery
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    • v.32 no.4
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    • pp.474-478
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    • 2005
  • Craniosynostosis is a congenital anomaly characterized by premature closure of cranial sutures. Surgical intervention should be performed during infancy. However, surgical correction of craniosynostosis remains bone defect and secondary angle occasionally. Currently, publications investigating solutions to bone defect and secondary angle created by cranioplasty are getting much interest. We have used $BoneSource^{(R)}$ which is relatively safe as an implantable substance for providing solutions for this problem. From June 2002 to January 2004, five children with craniosynostosis underwent frontocalvarial contouring using $BoneSource^{(R)}$ and concurrent cranial vault remodeling. The patient ages ranged from 8.0 months to 4.9 years(mean, 2.5 years). The quantity of $BoneSource^{(R)}$ implanted ranged from 10 to 25g, with a mean of 13g. This paper presents the first series of children treated with $BoneSource^{(R)}$ for frontocalvarial contouring in the surgical correction of craniosynostosis. No patients experienced any complications. Our results shows excellent retention of contour without causing asymmetry or irregularity. No visible evidence of interference with craniofacial growth were observed. Through our experiences, $BoneSource^{(R)}$ is found to be very useful for frontocalvarial contouring in children undergoing correction of craniosynostosis.

Chemical Compositions of the Observed Precipitation in Forest Area on the Border of Highway(Shingal, Seochun) (고속도로변 산림지역(신갈, 서천) 강우의 화학적 조성)

  • 김영채;정동준;김홍률
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.4 no.4
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    • pp.237-247
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    • 2002
  • Air pollution by acid pollutants is problematic in the whole world. Water acidification has already been deteriorating the forest ecosystem. This study was conducted to analyze the acidity and chemical composition of the open precipitation and throughfall at forests with various geographic locations in Korea. The results of this study are as follows; The open precipitation pH was lowest in Seochun. The throughfall pH showed some buffering capacity in only Quercus mongolica stands. In Pinus rigida(Shingal and Seochun) stands, there was little difference from the open precipitation. Chemical composition of the open precipitation for each sampling site showed that $Ca^{2+}$, N $H_{4}$$^{+}$ and S $O_{4}$$^{2-}$ concentrations had higher value than other ions, and except these ions, the small quantity of ions showed different properties to each site. Changes of ion concentrations in the throughfall showed a tendency to increase. ion concentrations of the throughfall increased with washout and nutrient leaching from the trees. In conclusion, the influence was extended to the pure zone, and the frequency of acid rain is increasing. But, if the deposition of pollutants exceeds the capacity of purification, it would damage forest ecosystem. Further investigations are necessary to identify tolerant tree species to acid pollutants.nts.