• BMB Reports
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• 제29권6호
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• pp.535-539
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• 1996

The activation of phospholipase D (PLD) catalyzes hydrolysis of phosphatidylcholine (PC) to phosphatidic acid (PA) and choline in plants as well as animals. To determine the presence of PLD in oat cells, we prepared inside-out plasma membrane and cytosolic fractions from oat tissues. PLD activities in both cytosol and plasma membrane were detected by ion chromatography method. The activity of PLD in plasma membrane was dependent upon $Ca^{2+}$ concentration and was heat stable. To investigate whether G-protein couples to PLD, the effects of $GTP{\gamma}S$ and $GDP{\beta}S$ on the PLD activity were measured. PLD activity was dramatically increased 300~400% in the presence of 50 ${\mu}M$ $GTP{\gamma}S$ but not in the presence of 50 ${\mu}M$ $GDP{\beta}S$. These results indicate that G-protein may be involved in regulation of PLD activity. To identify whether PLD is regulated by red light receptor, phytochrome, we irradiated red, far-red, or red/far-red/red light on oat protoplasts. PLD activity has increased 5-fold and 3-fold by treatment with red light and red/far-red/red light, respectively. In contrast, irradiation with far-red light had little or no effect on PLD activity. These results suggest that phytochrome regulates PLD activity through activation of G-protein in oat cells.

• 한국자원식물학회지
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• 제29권1호
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• pp.128-135
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• 2016

귀리 종자에 감마선을 조사한 후 발아율과 생존율 및 초기 생장 특성을 조사하여 돌연변이 유도를 위한 적정 방사선량을 구명하고, DNA 손상 정도를 평가 하였다. 연구의 결과, 발아율은 500 Gy 이상의 선량에서 급격히 감소하였고, 단순회귀분석으로 평가한 LD50 선량은 392 Gy였다. 감마선 조사 30일 후 초장, 생체중, 근장, 분얼수를 조사한 결과 대조구와 100∼200 Gy 처리구간 차이가 없었으며, RD₅₀ 선량은 초장과 생체중은 411 Gy로 동일하였고, 근장 403 Gy, 분얼수 394 Gy였다. LD₅₀과 RD₅₀ 선량을 바탕으로 결정한 귀리의 적정 조사선량은 300~400 Gy였다. Comet assay로 DNA 손상 정도를 측정한 결과, 파종 7일 후에는 모든 감마선 처리구가 대조구와 비교하여 DNA 손상이 크게 나타났으며, 파종 30일 후에는 300 Gy 이상의 처리구에서 대조구보다 유의성 있는 DNA 손상이 관찰되어 생육형질과 동일한 경향이었다. 선량과 생장, 코멧분석 결과간의 상관관계를 분석한 결과, 머리의 비율을 제외한 다른 조사 항목에서 높은 상관관계를 나타내었다. M₂ 세대의 생육특성은 400 Gy 이상에서 생육이 감소하였고, 돌연변이 양상은 주로 고선량에서 단간, 불임이었다.

• 한국잡초학회지
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• 제10권3호
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• pp.227-232
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• 1990

The effects of alachlor [2-chloro-2', 6' diethyl-N-(methoxymethyl) acetanilide] treatment on nucleic acid, amino acid and protein synthesis were studied. The amide herbicide alachlor blocks the biosynthesis of the amino acids isoleucine, valine and aromatic amino acid in oat root tips. Nucleic acid was inhibited, but was not proportional to reduction in protein synthesis. $1{\times}10^{-4}M$ of alachlor treatment of oat roots inhibited 36% DNA synthesis, but DNA synthesis was not inhibited at $1{\times}10^{-5}M$. RNA synthesis was inhibited by $1{\times}10^{-5}M$ and $1{\times}10^{-4}M$ of alachlor 16 and 27%, respectively, while inhibition of protein synthesis did occur at same concentrations. Inhibition of protein synthesis also did not occur at concentration below $1{\times}10^{-4}M$ alachlor. It suggest that inhibition of protein sythesis caused significantly by alachlor($1{\times}l0^{-3}M$) result from secondary action.

• 한국초지조사료학회지
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• 제16권4호
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• pp.307-314
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• 1996

An experiment was carried out to determine effect of cultivar and preservative on the characteristics and quality of baled oat(Avena sativa L.) hay after 60 days of storage. Ihe main plots consisted of the cultivars such as 'Foothill' and 'Swan', and the subplots consisted of preservatives such as commercial propionic acid(CA), lactic bacteria inoculant(LB) and control(C0). Bale temperature of 'Foothill' oat hay maintained higher than that of 'Swan' oat hay during storage. Bale temperature of oat hay treated with CA remained lowest during the earlier storage period and no significant difference was found among preservatives during the latter storage period. Visual assessment of bale of 'Swan' oat hay was better in the evaluation of mold, odor and color than that of 'Foothill' oat hay after storage. DM content of Foothill oat hay increased markedly than that of 'Swan' oat hay through the storage period. CFU(Colony fodng unit) of total viable micmrganism of oat hay bales was not quite different between preservatives, but that of lactic bacteria of 'Swan' oat hay and 'Foothill' was different by over 16 and lo4, respectively. Total nitrogen content of 'Swan' and 'Foothill' oat hay was 1.45 and 2.22%, respectively(P<0.05) and effect of preservatives on total N content was higher in the order of LB, CA, and CO. ADIN content of 'Swan' was lower than that of 'Foothill'(P

• Journal of Plant Biotechnology
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• 제45권4호
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• pp.375-381
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• 2018

귀리(Avena Sativa L., Oat)에서 이삭모양과 탈부특성이 다른 겉귀리와 쌀귀리를 품질특성으로 구분하고자 12 품종 귀리종실의 이화학적 특성 및 식이섬유를 분석하였고, 61개의 SSR 분자마커를 이용하여 근연관계 및 조양 판별 마커조합을 설정하였으며 결과를 요약하면 다음과 같다. 1. 공시한 12개의 귀리 품종을 생육조사 한 결과 쌀귀리는 이삭의 한 소수에서 영화수가 4~5개 생기는데 비해 겉귀리는 2~3개 생겼으며, 탈곡 후 종실의 껍질 유무 또한 쌀귀리는 없었고 겉귀리는 껍질이 남아있는 형태로 기존에 알려진 것과 같은 결과를 보였다. 2. 단백질은 쌀귀리가 13.45 ~ 16.56%, 겉귀리는 12.95 ~ 15.31%의 함량 분포를 나타내었으며, 지방은 쌀귀리가 7.26 ~ 10.34%, 겉귀리는 7.53 ~ 10.82%의 함량 분포를 나타내었으며, 베타글루칸은 쌀귀리가 4.19 ~ 5.05%, 겉귀리는 3.49 ~ 5.60%의 함량 분포를 나타내어 귀리 품종 간에는 품질특성이 차이가 나타났으나 쌀귀리와 겉귀리를 구분 할 수 있을 정도는 아니었다. 3. 지방산 조성 비율은 쌀귀리가 겉귀리에 비해 올레인산(C18:1, Oleic acid, 40.2 ~ 50.9%)과 스테아르산(C18:0, Stearic acid, 1.2 ~ 2.5%)이 높은 비율을 나타냈으며, 겉귀리는 쌀귀리에 비해 리놀레산(C18:2, Linoleic acid, 30.6 ~ 41.2%)과 리놀렌산(C18:3, Linolenic acid, 0.7 ~ 1.3%)이 더 높은 비율을 보였다. 4. 귀리의 근연관계는 계수(Coefficient) 1.22에서 그룹 하여 정리한 결과 겉귀리 6품종과 쌀귀리 1품종으로 그룹 되었고 유전적 거리(Genetic Distance)는 '동한'과 '태한'이 가장 가깝게 나타났으며 다음은 '풍한', '삼한', '옥한', '조풍', '중모2005' 순으로 나타났다. 계수(Coefficient) 1.22에서 그룹 되지 않은 귀리는 겉귀리 1품종과 쌀귀리 4품종으로 '수양', '다한', '대양', '선양', '조양' 순으로 그룹에서 먼 것으로 나타나 대체적으로 겉귀리와 쌀귀리가 서로 멀게 나타났다. 5. 12개의 귀리품종에 61개의 SSR maker를 이용하여 분석한 결과 '조양'은 AM28, 29, 32, 34, 36의 5개 SSR마커에서 다른 품종과 다른 밴드 패턴을 나타내어 품종 구분이 가능하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.183-183
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• 2022

This study was aimed to investigate the content of avenanthramides(AVNs) and other phenolic compounds in the grains of a naked oat cultivar, Daeyang, which were seeded in the fall and spring, and examine the effects of alcohol extracts from the grains on natural killer(NK) cell activity in vitro. The content of AVN-A, AVN-B, and AVN-C in the spring-seeded oat grains was 2.2 folds higher than the fall-seeded oat grains on average. Among these AVNs, the content of AVN-C was 1.9-folds higher in the spring-seeded oat grains(66.1㎍/g), comparing to the content in the fall-seeded oat grains(34.8㎍/g). The content of other phenolic compounds, such as phenolic acids and flavonoids in the spring-seeded oat grains was 1.1~4.7-folds higher than the fall-seeded oat grains. In particular, sinapinic acid was the most abundant phenolic acid in the spring-seeded oat grains(50.0㎍/g) and its content was 2.4-folds higher than its content in the fall-seed oat grains. Furthermore, NK cell activity in vitro treated with the spring-seeded oat grain extracts was 158%, and it was 18%p higher than NK cell activity treated with the extracts from the fall-seeded oat grain extracts. Our finding suggest that the bioactive properties of naked oat grains would be enhanced by spring seeding.

• 한국작물학회지
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• 제56권3호
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• pp.284-291
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• 2011

Varietal and annual variations in the contents of ${\beta}$-glucan fractions per weight grain samples were examined in sixteen covered and eighteen naked barley and five oat cultivars developed in Korea. Also, the effect of pearling on ${\beta}$-glucan content was investigated. Average contents of total, soluble and insoluble ${\beta}$-glucan fractions were 5.25, 3.72, and 1.53%, respectively, in covered barley, and 5.86, 3.51, and 2.35%, respectively, in naked barley. Soluble ${\beta}$-glucan content was higher in covered barley, though total ${\beta}$-glucan content higher in naked barley. The total and insoluble ${\beta}$-glucan contents were higher in pearled grains. Total ${\beta}$-glucan content was higher in waxy barley than in non-waxy barley. Duwonchapssalbori, a two-rowed and waxy naked barley cultivar, was highest in total, soluble and insoluble ${\beta}$-glucan contents. Highly significant positive correlations were observed between total ${\beta}$-glucan and soluble ${\beta}$-glucan contents both in covered and naked barley. There were significant annual variations in total ${\beta}$-glucan content in barley. Average contents of total, soluble and insoluble ${\beta}$-glucans of oat cultivars were 4.33, 3.44, and 0.89%, respectively. Contents of all fractions of ${\beta}$-glucans were higher in barley than in oat. These results would be useful for the breeding of high ${\beta}$-glucan variety and also for the use barley and oat as valueadded food ingredients.

• 한국잡초학회지
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• 제6권2호
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• pp.168-173
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• 1986

본 연구는 생장억제제(生長抑制製)인 fluazifop-butyl의 제초작용기구(除草作用機構)를 구명하기 위하여 본제초제가 생장의 기본요소인 세포분열(細胞分裂)과 신장(伸長) 그리고 단백질합성(蛋白質合成)에 미치는 영향을 조사하였다. 본제초제를 귀리의 뿌리에 농도별로 처리한 후 0 에서 48 시간까지의 세포분열(細胞分裂)에 미치는 영향을 조사하였다. 또한 세포신장(細布伸長)에 미치는 제초제의 영향은 oat coleoptile straight growth test 방법으로 조사하였다. 단백질함량(蛋白質含量)에 미치는 제초제의 영향은 $^{14}C$-leucine을 뿌리에 흡수 시켜서 합성인제합성유제(合成柳制) 정도를 liquid scintillation counter로 측정했다. 16시간 처리 후 $1{\times}10^{-3}M$과 $1{\times}10^{-4}M$구에서 세포분열을 억제하였다. 18 시간 처리 후 모든 처리구에서 세포분열이 억제되었다. 24 시간 처리 후에는 $1{\times}10^{-3}M$은 100%, $1{\times}10^{-4}M$은 99% $1{\times}10^{-5}M$은 89% 의 세포분열을 억제시켰으나 저농도구인 $1{\times}10^{-6}M$은 같은 처 리 기간동안에 20%의 세포분열만을 억제시켰다. 농도와 처리시간이 증가함에 따라 억제효과(抑制效果)도 증가하였다. 억제효과가 가장 크게 나타난 기간은 처리후 0 에서 18 시간 이내에 나타났다. 본 제초제의 세포신장억제 효과는 $1{\times}10^{-7}M$ 이상의 고농도에서 유의성(有意性)을 나타냈으며 $1{\times}10^{-6}M$ 이상의 고농도에서는 50% 이상의 세포 신장억제를 보여 주었다. 단백질합성에 관한 조사에서는 8 시간 처리후에 60%의 단백질합성이 억제되었다. 이상의 결과를 종합하여 볼 때 fluazifopbutyl의 식물생장(植物生長) 억제기구(抑制機構)는 세포분열과 신장 그리고 단백질합성을 억제함으로써 기인된 것으로 시료된다.

• 한국초지조사료학회지
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• 제17권2호
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• pp.101-109
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• 1997

Nine oat(Avena sativa L.) cultivars have been recommended as the govemment recommended forage cultivars since 1984, however, their forage performance and quality have mostly been tested at two locations, such as Suweon in the Middle Northwestem Coast Region and Sunghwan in the Middle Southwestern Coast Region. Therefore, the objective of this experiment was to retest the forage performance and quality of six government recommended oat cultivars at five scattered locations, such as Icheon, Suweon, Sunghwan, Keongsan and Kwangju fom 1992 to 1994 with the intention of improving recommendations to dairy farmers. Among the oat cultivars tested, "West" was the highest yielding cultivar at all locations in 1992-94, but the lowest yielding cultivars varied by location and year. At both Icheon and Suweon, "Cayuse", "Magnum" and "Foothill", at Sunghwan, "Cayuse" , "Ensiler" and "Foothill", at Keongsan, "Magnum", "Cayuse" and "Swan", and at Kwangju, "Cayuse", "Magnum" and "Cayuse", were the lowest yielding cultivars in 1993, 1994 and 1995, respectively. At all locations in 1992-94, "West" and "Swan" were the most advanced in growth stage, but "Cayuse", "Foothill", "Magnum" and "Ensiler" were the latest developing cultivars among the oats. Differences among the oat cultivars for the three-year mean NDF, ADF and IVDMD were small and inconsistent, but forage quality of the oats was generally lowest in the early developing cultivars and highest in the late developing cultivars. The govemment recommended oat cultivars tested were generally well adapted across the country, however, in forage production situations in the fall where high yield is more important, early maturing oat cultivars might be preferable to late cultivars because they have higher forage yield earlier in the fall.ction situations in the fall where high yield is more important, early maturing oat cultivars might be preferable to late cultivars because they have higher forage yield earlier in the fall.

• 한국육종학회지
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• 제41권2호
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• pp.168-172
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• 2009

'풍한'은 조숙 다수 월동가능 조사료용 귀리 품종을 육성할 목적으로 1999년에 조숙인 '식용귀리'와 대립종인 'PA202-210'을 교배한 $F_1$잡종에 내한성 계통인 'Beltsville 61-150'을 부본으로 인공교배하여 집단 및 계통육종법에 의해 계통을 선발한 후 2004년부터 생산력검정시험 및 지역적응시험을 거쳐 2008년에 육성된 품종으로 주요 특성은 다음과 같다. 1. '풍한'은 잎폭이 중 정도이며, 이삭형은 산수형이고, 종자색은 황백색이다. 2. '풍한'은 적응지역에서 출수기가 평균 5월 7일이며, 출수 후 20일에 평가한 생초수량이 ha당 평균 50.1 톤, 건물수량이 평균 14.5 톤으로서 기존 품종인 '삼한'보다 출수기가 2일 빠르고 조사료 수량도 높다. 3. '풍한'의 조사료 품질은 조단백질 함량이 6.7%, TDN이 60.3%로 표준품종인 '삼한귀리'와 대등하다.