• Journal of Mushroom
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• v.16 no.4
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• pp.311-317
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• 2018

The aim of this study was to identify chemical composition and antioxidant activity of phenolic extract of fruiting bodies of an artificially cultivated Hankyong Sanghwang mushroom, Phellinus linteus KACC 93057P (PLHS). The total phenolic content of 60% ethanolic extract of fruiting bodies of two-year-old PLHS grown on Oak wood logs was $19.05{\pm}0.32mg$ gallic acid equivalent (GAE)/g, which was 4-10 times high compared to the other species of mushrooms. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbisthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities and the ferric reducing antioxidant power (FRAP) value of PLHS were 2-10 and 5 times higher, respectively, than those of the other species of mushrooms. High performance liquid chromatography (HPLC) analysis of ethyl acetate fraction of 70% methanolic extract of the fruiting bodies of PLHS revealed the presence of styrylpyrone-class compounds, davallialactone, hispidin, hypholomine B, and caffeic acid, a compound of the phenylpropanoid pathway.

• International Journal of Industrial Entomology and Biomaterials
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• v.48 no.3
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• pp.147-155
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• 2024

This study aimed to validate the nutritional superiority and safety of fermented coffee byproducts (CB) and fermented oyster mushroom cultivation by-products (OMCB) as alternative food sources for Protaetia brevitarsis larvae. Thus, we conducted a comparative analysis of the nutrient composition of the food sources and developmental characteristics of the larvae. P. brevitarsis larvae have traditionally utilized various by-products as food sources and are able to efficiently utilize these. The analysis of the nutrient composition of the food sources indicated that, in comparison to control group fermented oak sawdust (FOS), the content of crude protein was 2.2 folds higher in larvae fed with OMCB and 3.2 folds higher in larvae fed with fermented 50% CB + 50% OMCB (CB + OMCB). Moreover, the cellulose content, utilized as a nutritional source for the larvae, was 1.3 folds higher in the CB + OMCB group than in the control group. Significantly, the weight of larvae fed with OMCB and CB + OMCB increased 1.7-4.2 folds compared to those fed with FOS. Additionally, the survival rate of larvae before the formation of pupal cells was over 90% in all groups. Therefore, it was concluded that CB and OMCB contain various nutrients without harmful composition and have a larval growth-promoting effect. Consequently, they are considered appropriate dietary materials for P. brevitarsis larvae. This study enhances our understanding of by-product usages by P. brevitarsis larvae and confirms their potential as sustainable food resources.

• The Korean Journal of Mycology
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• v.22 no.3
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• pp.222-228
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• 1994

In Lentinus edodes(oak mushroom) cultivation, commonly are logs and sawdusts of oak and some other broadleaved tree species used. Recently oak trees have been substantially diminished due to extensive logging. Thus, to develop comparable synthetic formula using other tree species for the cultivation of Lentinus edodes, we investigated the effect of various nutrients and pretreatment on L. edodes mycelial growth in coniferous sawdusts(i.e., Pine and Larch). We found that 1.5 hr pretreatment of sawdust with hot water and adding 10% rice bran, 3% charcoal, 0.02% $NH_4CI$ and 0.5-1% lignosulfornic acid were effective for the growth of L. edodes in pine sawdust media. In larch sawdust pretreatment with acetone for one hr and adding 20% rice bran, 3% charcoal and 0.02% $NH_4CI$ increased L. edodes mycelial growth. We also analyzed the components of oak and coniferous sawdusts and found oak has higher content of xylose and lower content of lignin, arabinose and mannose than conifers. Rice bran, compared with BITEL(HOKKEN Co.) known for better commercial substitute for rice bran, has lower content of xylose and galactose, but the similar C/N ratio.

• Journal of the Korean Wood Science and Technology
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• v.32 no.1
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• pp.35-44
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• 2004

The water soluble fractions(WSF) from Japanese larch wood were isolated, purified by anion exchange resin and Sephadex gel filtration and identified its chemical structure by means of periodate oxidation and methylation reactions. Its major components are arabinose and galactose (1 : 3.4). Based on the results of periodate oxidation, methylation and gas chromatographic analysis of purified WSF, main chain is composed of β-1,3-glycosidic linkage among D-galactopyranoses, and two different side chains; β-1,6-glycosidic linkage among 2-3 units of D-galactopyranoses and β-1,6-glycosidic linkage between 1-2 units of D-galactopyranose and L-arabinopyranose. Addition of WSF to culture media of oak mushroom (Lentinula edodes) accelerated the mycelial growth. In the case of PDA cultures, 2 percent addition of WSF in Sanlim No. 6 strain and 4 percent of WSF in Mok-H strain mostly enhanced the mycelial growth of the mushroom. In the case of sawdust cultures, 4 percent addition of WSF in two strains showed the best mycelial growth. High percentages addition of WSF inhibited mycelial growth of the mushroom. Mushroom production was increased with addition of WSF. By the addition of WSF, ergosterol contents in the media were quite high at the colonized stage and rapidly increased at the fruiting stage. Therefore the ergosterol content could be utilized as an indicator to evaluate the culture maturity for the mushroom fruiting.

• Journal of Mushroom
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• v.21 no.3
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• pp.154-159
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• 2023

A new spore-less cultivar Lentinula edodes 'Daedam' was bred from monokaryotic strains of 'LE15401-24' and 'LE192118-10'. The optimum temperature for mycelial growth of 'Daedam' on potato dextrose agar was 22~25℃. Total cultivation period of the new cultivar, from inoculation to its first harvest, was 134 days, similar to that of the control cultivar 'Hwadam'. Total yield of 'Daedam' was 222g per 3kg substrate, and was lower than that of control cultivar(266.0g). The fruiting body of 'Daedam' had a thick and small pileus and a longer stem compare to control cultivar. As a result of a analyzing the productivity of 'Daedam' on the different substrate types, the biological efficiency was 26.7% in the 1.2kg cylindrical substrate(CS), which was higher than that of the 3kg rod-type substrate(RS). 'Daedam' had a similar yield compared to 'Hanacham' in first fruiting body production, but the cultivation period was 40 days shorter. Therefore, 'Daedam' can only harvest fruiting bodies once, it is thought that it can be used as spore-less oak mushroom cultivar for short-term cultivation instead of 'Hanacham' in mushroom farms.

• Journal of the Korean Wood Science and Technology
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• v.16 no.3
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• pp.38-47
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• 1988

표고버섯(Lentinus edodes) 균에 의한 졸참나무(Quercus serrata)골목의 미시형태적 변화를 주사형전자현미경 (SEM)을 통해 관찰하였다. 표고 재배 5년후 골목의 중량 감소율은 50%에 달했다. 구성세포중 목섬유는 표고균사에 의해 쉽게 분해되었으나 도관과 유세포는 저항성을 나타냈다. SEM 관찰 결과 표고균사는 세포내강(內腔)에서부터 세포벽을 서서히 분해 박벽화(薄壁化)시켰으며, 또한 Cell corner가 중간층보다 먼저 공격을 받아 분해 되었으나 중간층은 쉽게 분해되지 않았다. 흥미롭게도 연부후(軟腐朽)의 전형적 형태인 2차막에서의 공동(空洞)역시 백색부후균인 표고균사에 의해 형성 되었다. 이상의 관찰결과 표고균은 백색부후와 연부후균이 갖는 미시형태적 특징을 동시에 보여 주었다. 아울러 중간층 보다 Cell corner가 먼저 선택적으로 분해되며, 도관세포벽의 분해저항성을 세포벽 구성 리그닌의 화학적 특성과 연관시켜 논의했다.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2003.02a
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• pp.309-314
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• 2003

표고버섯은 특이한 향과 육질, 약효성분 때문에 오래 전부터 식용으로 애용된 임산물이다. 표고버섯은 원래 한국, 중국, 일본에서만 자생하던 극동지역 특산물로서 미국, 유럽 등지에서는 발견되지 않았으나, 현재는 전 세계에서 광범위하게 인공재배 되고 있다. 표고버섯에 포함되어있는 수용성 다당류인 렌티난(lentinan)은 종양세포인 Sarcoma-180의 생장을 저해하는 항암효과가 있는 것으로 알려져 있다(최 등 1995). 또한 여러 종류의 유리 아미노산이 들어 있고 열량이 낮은 반면, 비타민과 식이섬유 등이 풍부한 영양식품이다(황 1983). 그러나 버섯은 함수율이 높고 조직이 연약하여 저장하기 어렵고, 부패되기 쉽기 때문에 수확 후 단시일 내에 생버섯으로 판매하거나, 통조림 또는 건조가공하고 있지만, 후자가 일반적이다(하 등 2001). (중략)

• Journal of the Korean Society of Food Science and Nutrition
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• v.19 no.1
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• pp.73-79
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• 1990

In order to study on the riboflavin content of common foods for Korean and the rates of destruction of riboflavin during cooking 26 kinds of the foods were selected and 3 kinds of menu were cooked by standardized method. For each food item and menu riboflavin content was measured by AOAC method. The experimental values of 13 kinds of food such as rice oak mushroom carrot squarsh tangle dried large anchovy apple(Fuji) dried laver ramyun pork soybean curd fried soybean curd and danmugi were almost consistent with food compo-sition values. Whereas those of 12 kinds of foods such as cabbage onion potato kimchi beef sausage dried medium anchovy hair tail soybean paste and egg were considerably different from food composition table values, up to now Alaskan pollack maize loaf bread hamburger bread etc have not been analyzed in food composition table, The rates of retention of riboflavin in menu 1, 2, and 3 cooked by standardized method were 24% 69% 46% respectively. The overally retention rate was in inverse proportion to the time of sunlight exposure during cooking.

• Mycobiology
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• v.41 no.1
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• pp.42-46
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• 2013

The effects of the composition of a mixture containing food waste compost (FWC), rice bran (RB), and oak sawdust (SD) on the antler-type fruiting body (FB) yield of Ganoderma lucidum were studied. Experiments were performed using 0 (control), 5, 10, 15, 20, 25, 30, 35, and 40% (w/w) FWC added to a basal growth medium consisting of 20% (w/w) RB and 80% (w/w) SD. The content of 15% FWC gave the highest FB yield ($27.0{\pm}1.3$ g/bottle), which was 44% higher than the yield ($18.6{\pm}2.8$ g/bottle) of the control treatment. However, FWC contents of 20~40% showed reduced yield (2.4~23.0 g/bottle), partly because FWC had a high Na concentration (0.6%). These results demonstrate the potential for use of FWC as a component of a growth medium for production of G. lucidum FBs.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1999.11a
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• pp.114-117
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• 1999

표고 시설재배에서의 고품질 표고(동고, 화고)의 생산환경 분석을 위하여 차광재배와 시설재배사의 환경을 분석하여 각 시설내의 환경조건을 분석하였다. 차광재배사와 시설재배사의 기상환경 분석 결과, 시설재배의 습도는 차광재배에 비하여 20-40%높게 나타났으며, 70-80% 이상인 기간이 차광재배에 비하여 약2배 가량 높게 나타났다. 생육기간중 시설재배에서의 최고온도가 저온성 표고의 생육 온도보다 2-4$^{\circ}C$ 높아 생육에 악 영향 준 것으로 판단된다. 골목 하부로 갈수록 시설재배에서 4-5$^{\circ}C$ 높게 나타났다. 시설재배의 $CO_2$의 농도는 차광재배에 비하여 100-200ppm 정도 높게 나타났다. 이상의 결과로부터 시설내의 환기촉진과 과습방지가 시설내의 고품질 표고 생산을 위한 환경개선 방법이라고 판단된다.