• Journal of Forest and Environmental Science
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• 제34권5호
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• pp.395-404
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• 2018

Since the mass mortality of Quercus mongolica has been first reported in Gyeonggi province at 2004, the disease spread rapidly over Korean peninsula annually. Ambrosia beetle (Platypus koryoensis) was known as the insect vector of oak wilt fungus, Raffaelea quercus-mongolicae, and control methods of the disease had mainly been focused on eradication of insect vector. However, for the efficient management of the disease, combined control methods for both of the pathogenic fungus and insect vector are strongly required. As one of the efforts to suppress the pathogenic fungus, antifungal activities of Streptomyces isolated from oak forest soil were assayed in this study. Optimum culture condition for the selected isolates was also studied, As a result, Streptomyces blastmyceticus cultured in PDB (Potato Dextrose Broth) at $25^{\circ}C$ for 1 week showed the strongest antifungal activity against oak wilt fungus. Mycelial growth inhibition rates (MGIRs) of Streptomyces isolates were compared on culture media supplemented with heated and unheated culture filtrates of S. blastmyceticus. MGIRs on culture media with unheated culture filtrates were generally higher than those on culture media with heated culture filtrates. Antagonistic mechanism to get involved in the inhibition of hyphal growth and spore formation of the pathogen is due to the antifungal metabolites produced by Streptomyces. This study will provide the fundamental information in developing biocontrol agents for the environment-friendly management of oak wilt disease.

• 한국잠사곤충학회지
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• 제36권2호
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• pp.130-137
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• 1994

천잠의 사육환경을 구명하기 위하여 사료자원, 기상환경 및 사육방법등에 관하여 조사한 결과를 다음과 같이 요약한다. 1. 천잠의 실내사육에 있어 사육온도는 항온보다 변온이 좋은 편이며, 치잠기 25~26$^{\circ}C$, 장잠기 22~23$^{\circ}C$ 범위가, 사육습도는 치잠기 80~90%, 장잠기 60~70% 범위가 적당한 것으로 나타났다. 2. 천잠사육에 있어 광조기는 유충의 성장에 미치는 영향이 크지 아니하였다. 3. 귀잠시기는 빠른것이 늦은 것보다 유충시기에 단축되는 경향이 있으며 질도 양호한 것으로 나타났다. 4. 사료수종은 상수리나무가 밤나무보다 더 적합한 것으로 나타났다. 야생수보다 재배수가 사육성육이 더 좋았다. 5. 천잠을 옥외사육으로 전환하는 시기는 빠를수럭 작황이 불안정해 지는 경향을 보였다.

• Journal of Forest and Environmental Science
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• 제33권4호
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• pp.342-347
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• 2017

Since the first report of the oak wilt disease at 2004 in Korea, the disease distributed over Korean peninsula and are still giving severe damages. The management of oak wilt disease in Korea has mainly focused on the control of insect vector, Platypus koryoensis. Neverthless the effective method for evaluating the pathogenicity of the pathogen, Raffaelea quercus-mongolicae (Rqm), and for screening chemical or biological agents with strong inhibitory activity against the pathogen, is absolutely necessary, an reliable method is not available so far. This study was conducted to develop the effective method for evaluating the pathogenicity of Rqm in oak trees. The culture suspensions of Rqm were artificially injected to the saplings of Quercus acutissima by using ChemJet tree injector. Three months after treatments, the treated saplings were cut and dipped into 1% fuchsin acid solution. There were significant differences in non-conductive area (%), discoloration area (%) and vertical discoloration length between the pathogen-injected and distilled water-injected control treatments. These results indicated that the pathogen is the causal agent for the dysfunction of water conductive tissue, which will finally result in wilt symptom. Re-isolation of the pathogen and PCR detection using specific primers for the pathogen also confirmed the presence of Rqm in the sapwood chips of the pathogen-injected saplings. These observations would be greatly applied to other related researches for evaluating the pathogenicity of tree wilt pathogens and biocontrol efficacy of the selected antagonistic microorganisms, in case that the wilt symptom is not easily shown by artificial inoculation of the causal agent.

• 조경수
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• 통권113호
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• pp.18-19
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• 2009

• 한국응용곤충학회지
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• 제47권2호
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• pp.127-131
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• 2008

참나무시들음병의 매개충인 광릉긴나무좀, Platypus koryoensis(Murayama)은 한국에서 참나무시들음병원균인 Raffaelea sp.를 매개하는 것으로 알려져 있다. 참나무시들음 발병정도는 광릉긴나무좀의 밀도에 의존적인 것으로 추정되고 있다. 이에 (樹幹)내 천공수와 집중도가 참나무 피해정도에 미치는 영향을 구명하기 위하여 본 연구를 수행하였다. 수락산 피해지에서 고사목 6그루와 피해목 28그루의 신갈나무의 피해정도, 단위면적당 천공수, 천공간 최근거리를 수간의 상부(지표로부터 50cm)와 하부(지표면)에서 조사하였다. 상부와 하부에서의 천공수는 양의 상관을 보였으며 천공간 최근거리 또한 같은 경향을 보였다. 천공수가 증가할수록 수목의 피해도가 심하였으나 수목의 피해도가 심할수록 천공간 최근거리는 감소하였다. 천공의 분포도 수목의 피해도가 증가할수록 집중분포에서 군일분포로 바뀌었다. 광릉긴나무좀이 초기에는 집중적으로 공격을 하나 수간내 밀도가 증가함에 따라 종내경쟁이 일어나고 경쟁의 결과 개체간 간섭현상이 유도되고 천공의 공간적 분포가 균일하게 변환하게 된다는 것을 암시하는 것이다.

• Journal of Forest and Environmental Science
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• 제34권5호
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• pp.376-381
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• 2018

For the control of oak wilt caused by Raffaelea quercus-mongolicae, an antifungal microorganism, Streptomyces blastmyceticus, was used as a potential agent. Culture suspension of S. blastmyceticus was injected into Quercus mongolicae in the research forest of Kangwon National University by $ChemJet^{(R)}$ trunk injection and Macro-infusion at root flare injection. $Alamo^{(R)}$ (a.i., propiconazole 14.5%), a fungicide currently used for the control of oak wilt in USA, was also treated by both methods to compare the efficacy. For preventive efficacy, culture suspension of the pathogen was inoculated at 1 month after injection of either agent. Tested trees were cut at 3 months after treatment, stained with 1% Fuchsin acid, and then non-conductive area (NCA) and re-isolation frequency (RIF) of oak wilt fungus were compared among treatments. While NCA was the highest as 47.3% in pathogen only treatment, it was the lowest as 16.0% in sterilized water treatment by Macro-infusion. NCAs of Alamo treatment by Macro-infusion and ChemJet injection were 25.3% and 32.1%, respectively. NCA of S.blastmyceticus treatment by ChemJet injection was 32.3%, similar with Alamo treatment's by ChemJet injection. All treatments by either injection method showed significantly lower NCA compared to the pathogen only treatment. These results indicate that S. blastmyceticus injection shows the preventive efficacy against oak wilt disease by suppressing the growth of pathogen injected. NCA of Macro-infusion injection of sterilized water was lower as 16.0%, compared to 21.3% of ChemJet injection. It means that Macro-infusion is more effective in translocation of sterilized water than ChemJet injection by even distribution. RIF from wood discs of treated trees showed high in pathogen only treatment, but relatively low in S. blastmyceticus treatment. RIF results were correlated with NCA results. From the above results, it was confirmed that S. blastmyceticus showed preventive efficacy against oak wilt disease by ChemJet trunk or Macro-infusion at root flare injection.

• 한국환경복원기술학회지
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• 제13권3호
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• pp.36-45
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• 2010

To analyze the relationship between climatic factors (monthly mean temperature and total precipitation) and tree-ring growths of Quercus mongolica Fischer (Mongolian oak) with different topographic sites in Mt. Worak, more than 10 trees were selected from each of seven stands. Two cores from each tree were measured for ring width. After crossdating, each ring-width series was double standardized by fitting first a negative exponential or straight regression line and secondly a 60-year cubic spline. Seven stands were categorized in two groups using cluster analysis for tree-ring index patterns. Cluster I (four stands) was located in higher elevation (550-812 m) with aspects of east, west and northwest, and cluster II (three stands) was located in rather lower election (330-628 m) with aspects of north and northwest. The aspects of two clusters were not significantly different. Response-function analysis showed a significant positive response to March precipitation for both clusters. It indicates that moisture supply during early spring season is important to radial growth because the cambial growths of ring-porous species, such as Mongolian oak, start before leaf growth. Cluster II showed a positive response to the precipitation of middle and late growing season, too.

• Journal of the Korean Wood Science and Technology
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• 제44권4호
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• pp.515-525
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• 2016

The Palsangjeon Pagoda is a five-story wooden building in the Beopjusa Temple area in the Songnisan national park in Chungcheongbuk-do, Korea. According to historical records, it was constructed in either AD 1605 or AD 1626. To specify the construction year more precisely, we took 41 wood samples for tree-ring analysis during repair actions in 2013; 28 from red pine (Pinus densiflora S. et Z.), 12 from oak (Quercus spp.), and 1 from fir (Abies spp.). The tree-ring boundaries became clearly visible by smoothing their cross-sectional surface and the tree-ring widths were measured under a stereo microscope. All tree-ring series were compared with each other to establish mean chronologies, which we then compared with local master chronologies established using tree rings collected from wooden elements of the Daeungjeon Hall which is the main building in the Beopjusa Temple area. As a result, it was verified that the red pines were felled in AD 1624 and in AD 1851. Moreover, it was verified that the oaks were also felled in AD 1624 based on cross-dating with the red pine chronology. We concluded that the Palsangjeon Wooden Pagoda was constructed in AD 1626, as mentioned in the historical record (Daeungdaekwangmyeongjeonbulsangki, 1630) and the letters on a purlin on the $4^{th}$ story, using oak and red pine felled in AD 1624 and repaired later on using red pine felled in AD 1851.

• Journal of Forest and Environmental Science
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• 제29권1호
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• pp.29-37
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• 2013

The study was carried out in two different forest types viz., Banj oak and Chir pine forests to assess the variation in forest species composition and soil properties along altitudinal gradients in the Garhwal Himalayas. The results of the study showed that between the forests soil moisture was higher in Banj oak forest because of closed canopy and dense forest compared to Chir pine forest. The sand particles were reported higher in Banj oak forest which might be due to the addition of organic matter favouring coarse structure of soil, helping in holding maximum water in soils. However in the Chir pine forest low amount of soil organic matter and presence of clayey soil, develops soil compactness which reduces the penetration of water resulting in high soil bulk density. The higher accumulation of litter and presence of moisture in Banj oak forest favours higher nutrient level of nitrogen, phosphorus and potassium compared to Chir pine forest. The soil organic carbon also reduced with increasing altitude at both gradients. While bulk density has reverse trend with soil organic carbon in both the forests at different peaks of same region. In Banj oak forest, the highest density and total basal cover was reported 1,100 tree $ha^{-1}$ and 58.86 $m^2\;ha^{-1}$ respectively. However, the highest values of density and total basal cover of Chir pine forest was 560 tree$ha^{-1}$ and 56.94 $m^2\;ha^{-1}$ respectively. The total density and basal cover of both the forests reduced with increasing altitude. The study concludes that Banj oak forest has better nutrient cycling ability, well developed foest floor and has a greater protective and productive features compared to the Chir pine forest which is without lower vegetation cover and having only pine litter accumulation which does not allow any other species to grow.

• 생태와환경
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• 제38권4호통권114호
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• pp.475-481
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• 2005

본 연구는 새로운 환경친화적 살조제를 탐색하기 위한 연구의 일환으로, 담수에서 조류 대발생 원인종 Microcystis aeruginosa.를 대상으로 하여 국내에 자생하는 9가지 참나무 잎과 줄기로부터 추출한 물질의 조류생장 억제능력을 조사하였다. 추출물 농도 100 mg $L^{-1}$ 첨가시, 5가지 참나무 추출물 (QAT-L, QAT-S, QAS-L, QGI-S 및 QSA-L)은 M. aeruginosa의 7일 후 세포수를 90% 이상 감소시켰다. 20 mg $L^{-1}$의 경우에는 상기의 5가지 참나무 추출물에서 M. aeruginosa의 세포수가 약 50% 정도 감소하였다. M. aeruginosa를 효과적으로 제어하기 위한 참나무 추출물의 최소 농도는 20 mg $L^{-1}$로 조사되었다. 참나무의 주요 성분으로 알려져 있는 tannic acid는 17 mg $L^{-1}$의 농도에서 M. aeruginosa에 대한 억제효과를 나타냈다. 본 연구를 통해서 참나무 추출물은 부영양 수계에서 조류 대발생 원인종 M. aeruginosa를 제어하는 환경친화적 살조제로서 이용할 수 있을 것으로 판단된다.