• Title/Summary/Keyword: OPG/OPA

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Picosecond optical parametric generation and amplification using BBO crystals (BBO 결정을 이용한 Pico초 광매개 발생과 증폭)

  • 유난이;최혜영;차명식
    • Proceedings of the Optical Society of Korea Conference
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    • 2000.08a
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    • pp.244-245
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    • 2000
  • 비선형광학 결정을 이용한 optical parametric generation(OPG)은 coherent tunable radiation을 얻는 방법으로 매우 효과적이다. Beta barium borate(BBO) 결정은 비선형성이 클 뿐만 아니라 광손상문턱이 높고 자외선에서부터 근 적외선영역까지(2 -0.2$mu extrm{m}$) 투명하여 optical parametric oscillation(OPO)이나 OPG/OPA(optical parametric generation/amplification)에 많이 활용되고 있다.[1-2] 분광학적 연구와 여러 가지 응용 측면에서 넓은 영역의 파장가변이 손쉽고 높은 에너지를 갖는 pico 초coherent source는 중요하며, 이는 결정의 비선형성을 이용하여 공진기를 구성하지 않고서도 OPG/OPA를 통하여 실현 가능하다. (중략)

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Consistency of QTLs for Soybean Seed Size across Generations (대두 종자크기에 대한 QTL의 consistency)

  • ;James E. Specht
    • Journal of Life Science
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    • v.7 no.4
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    • pp.358-360
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    • 1997
  • Soybean [Glycine max (L.) Merr.] seed size is a important yield component and is a primary consideration in the development of cultivars for specialty markets. Our objective was to examine the consistency of QTLs for seed size across generations. A 68-plant F$_{2} segregation population derived from a mating between Marcury (small seed) and PI 467.468 (large seed) was evaluated with RAPD markers. In the F$_{2} plant generation (i.e. F$_{3} seed), three markers, OPL09a, OPM)7a, and OPAC12 were significantly (P<0.01) associated with seed size QTLs. In the F$_{2} ; F$_{3} generation (i.e., F$_{4} seed), four markers, OPA092, OPG19, OPL09b, and OPP11 were significantly (P<0.01) associated with seed size QTLs. Just two markers, OPL09a, and OPL09b were significantly (P<0.05) associated with seed size QTLs in both generations. The consistency of QTLs across generations indicates that marker-assisted selection for seed size is possible in a soybean breeding program.

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Assessment of Genetic Variability in Two North Indian Buffalo Breeds Using Random Amplified Polymorphic DNA (RAPD) Markers

  • Sodhi, M.;Mukesh, M.;Anand, A.;Bhatia, S.;Mishra, B.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.9
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    • pp.1234-1239
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    • 2006
  • Murrah and NiliRavi are the important North Indian buffalo breeds occupying the prominent position of being the highest milk producers. These breeds are more or less similar at morphological as well as physiological levels. The technique of RAPD-PCR was applied in the present study to identify a battery of suitable random primers to detect genetic polymorphism, elucidation of the genetic structure and rapid assessment of the differences in the genetic composition of these two breeds. A total of 50 random primers were screened in 24 animals each of Murrah and NiliRavi buffaloes to generate RAPD patterns. Of these, 26 (52%) primers amplified the buffalo genome generating 263 reproducible bands. The number of polymorphic bands for the 26 chosen RAPD primers varied from 3 (OPG 06 and B4) to 26 (OPJ 04) with an average of 10.1 bands per primer and size range of 0.2 to 3.2 kb. DNA was also pooled and analyzed to search for population specific markers. Two breed specific RAPD alleles were observed in each of Murrah (OPA02 and OPG16) and NiliRavi (OPG09) DNA pools. RAPD profiles revealed that 11 (4.2%) bands were common to all the 48 individuals of Murrah and NiliRavi buffaloes. Pair-wise band sharing calculated among the individual animals indicated considerable homogeneity of individuals within the breeds. Within breed, band sharing values were relatively greater than those of interbreed values. The low genetic distance (Nei's) value (0.109) estimated in this study is in accordance with the origin and geographical distribution of these breeds. The RAPD analysis indicated high level of genetic similarity between these two important North Indian buffalo breeds.

Two-Dimensional Correlation Analysis of Sum-Frequency Vibrational Spectra of Langmuir Monolayers

  • Lee, Jonggwan;Sung, Woongmo;Kim, Doseok
    • Journal of the Optical Society of Korea
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    • v.18 no.5
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    • pp.558-563
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    • 2014
  • Sum-frequency generation spectra of a Langmuir monolayer on water surface at varying surface areas were studied with two-dimensional correlation analysis. Upon enlarging the area/molecule of the Langmuir monolayer, the sum-frequency spectra changed reflecting the conformation change of the alkyl chains of the molecules in the monolayer. These changes stood out more clearly by employing two-dimensional correlation analysis of the above sum-frequency spectra. Features not very pronounced in the original spectra such as closely-spaced spectral bands can also be easily distinguished in the two-dimensional correlation spectra.

Genetic Diversity Evaluation of Thamnocalamus spathiflorus (Trin.) Munro Accessions through Morphological and Randomly Amplified Polymorphic DNA (RAPD) Markers

  • Tiwari, Chandrakant;Bakshi, Meena;Gupta, Dinesh
    • Journal of Forest and Environmental Science
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    • v.35 no.2
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    • pp.90-101
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    • 2019
  • Biodiversity refers to the total number and variation among species of flora and fauna of an area. Due to tremendous biotic especially anthropogenic pressure these natural resources are being vanishing. In present study genetic diversity among accessions of Thamnocalamus spathiflorus was evaluated. A total of 51 vegetative characters and 42 primers (10-mer) were screened. Out of 42 screened primers, 28 polymorphic primers were selected for further analysis. A total of 263 bands were recorded as polymorphic whereas 48 bands were monomorphic. The resolving power (Rp) of 28 Randomly Amplified Polymorphic DNA (RAPD) primers ranged from 4.6 (OPE08) to 17.6 (OPA11). The polymorphic information content (PIC) value ranged from 0.21 (OPAH09) to 0.44 (OPG02). The result revealed high degree of genetic relatedness (56 to 80%). Cluster analysis revealed two major clusters both for morphology as well as RAPD. Unlike morphological characterization, the accession (D5) from Bahli, Rampur, Shimla (H.P.) was clustered separately from the others in RAPD cluster analysis. Accessions with closed locality grouped together through RAPD marker system however analogy was recorded for morphological traits. The study conducted reflects the utility of RAPD technique for species identification and phylogenetic studies in bamboo for conducting bamboo breeding program.

QTL Analysis of Soybean Seed Weight Using RAPD and SSR Markers

  • Chung, Jong-Il;Ko, Mi-Suk;Kang, Jin-Ho
    • Plant Resources
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    • v.3 no.3
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    • pp.184-193
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    • 2000
  • Soybean [Glycine max (L.) Merr.] seed weight is a important trait in cultivar development. Objective of this study was to identify and confirm quantitative trait loci (QTLs) for seed weight variation in the F2 and F2:3 generations. QTLs for seed weight were identified in F2 and F2:3 generations using interval mapping (MapMaker/QTL) and single-factor analysis of variance (ANOVA). In the F2 plant generation (i.e., F3 seed), three markers, OPL9a, OPM7a, and OPAC12 were significantly (P<0.01) associated with seed weight QTLs. In the F2:3 plant row generation (i.e., F4 seed), five markers, OPA9a, OPG19, OPL9b, OPP11, and Sat_085 were significantly (P<0.01) associated with seed weight QTLs. Two markers, OPL9a and OPL9b were significantly (P<0.05) associated with seed weight QTLs in both generations. Two QTLs on USDA soybean linkage group C1 and R were identified in both F2 and F2:3 generations using interval mapping. The linkage group C1 QTL explained 16% of the variation in seed weight in both generations, and the linkage group R QTL explained 39% and 41% of the variation for F2 and F2:3 generation, respectively. The linkage group C2 QTL identified in F2:3 generation explained 14.9% of variation. Linkage groups C1, C2 and R had previously been identified as harbouring seed size QTLs. The consistency of QTLs across generations and populations indicates that marker-assisted selection is possible in a soybean breeding program.

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