• Title/Summary/Keyword: OMP

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In Vitro Selection of RNA Aptamer Specific to Salmonella Typhimurium

  • Han, Seung Ryul;Lee, Seong-Wook
    • Journal of Microbiology and Biotechnology
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    • v.23 no.6
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    • pp.878-884
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    • 2013
  • Salmonella is a major foodborne pathogen that causes a variety of human diseases. Development of ligands directly and specifically binding to the Salmonella will be crucial for the rapid detection of, and thus for efficient protection from, the virulent bacteria. In this study, we identified a RNA aptamer-based ligand that can specifically recognize Salmonella Typhimurium through SELEX technology. To this end, we isolated and characterized an RNase-resistant RNA aptamer that bound to the OmpC protein of Salmonella Typhimurium with high specificity and affinity ($K_d$ ~ 20 nM). Of note, the selected aptamer was found to specifically bind to Salmonella Typhimurium, but neither to Gram-positive bacteria (Staphylococcus aureus) nor to other Gram-negative bacteria (Escherichia coli O157:H7). This was evinced by aptamer-immobilized ELISA and aptamer-linked precipitation experiments. This Salmonella species-specific aptamer could be useful as a diagnostic ligand against pathogen-caused foodborne sickness.

Orthogonal matching pursuit via candidate supports (후보 support를 이용한 직교 매칭 퍼슛 알고리듬)

  • Kwon, Seok-Beop;Park, Jung-Yong;Lim, Chae-Hee;Shim, Byong-Hyo
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2012.07a
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    • pp.473-474
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    • 2012
  • Sparse한 신호 복원 방법으로 underdetermined system에서 ll-minimization을 이용한 compressive sensing의 연구와 함께, ll-minimization비에 간단한 greed 알고리듬도 활발히 연구되고 있다. 이에 본 논문은 greed 알고리듬의 대표적인 orthogonal matching pursuit기법에서 iteration 마다 후보 support를 유지하는 알고리듬을 연구한다. 모의 실험을 통해 OMP의 iteration 단계에서 하나의 support만 선택하는 것보다 후보 support를 유지하는 것이 sparse 신호를 복원하는 경우는 OMP와 비슷한 성능을 보이지만 덜 sparse한 신호복원에서는 더 좋은 성능을 보임을 확인 할 수 있다.

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Expression of Newer Outer Membrane Proteins (OMPs) Induced by Cephalosporins and Quinolone Group of Antibiotics in Klebsiella pneumoniae

  • KY TO;DANA VAN;SHARMA SAROJ;CHHIBBER SANJAY
    • Journal of Microbiology and Biotechnology
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    • v.15 no.2
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    • pp.421-424
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    • 2005
  • Effect of antibiotics belonging to three different groups, including third generation cephalosporins, aminoglycosides, and quinolones, on the outer membrane protein (OMP) profile of Klebsiella pneumoniae was examined. It was found that a new OMP (porins) of 40 kDa molecular mass was expressed in Klebsiella pneumoniae, when grown in the presence of ceftazidime, whereas new proteins with 30 kDa and 22 kDa masses were detected in the presence of ofloxacin. The immunoblot analysis showed that the new proteins of 40 kDa and 30 kDa molecular masses were expressed on the outer envelope, when being exposed to antibiotics ceftazidime and ofloxacin, respectively. This finding is important, as the outer surface comes in contact with the immune system, and therefore may have a bearing on the outcome of the disease.

Survey on the distributions of swine pleuropneumonia antibodies by ELISA in Kyongbuk western area (효소면역흡착시험을 이용한 경북서부지역의 돼지 흉막폐렴에 대한 항체분포조사)

  • 서희진;배성수;김대원;김봉환
    • Korean Journal of Veterinary Service
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    • v.23 no.3
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    • pp.289-299
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    • 2000
  • The study was performed to investigate the distributions of swine pleuropneumonia in Kyongbuk western area by the enzyme-linked immunosorbent assay (ELISA). Sera collected from 400 slaughtered pigs in 3 slaughter houses during the period from May 1999 to october 1999 were tested to detect antibodies against A pleuropneumonie serotype 2 and 5. The optimal dilution of CBE antigen, conjugate and serum for this ELISA were determined 1 : 400, 1 : 20,000, 1 100, respectively. The optimal dilution of OW antigen, conjugate and serum for this ELISA were determined 1 : In, 1 : 20,000, 1 : 200, respectively. Cut-off value in this ELISA was determined by mean absorbance (at 492 nm) of negative control sera added with the triple value of the standard deviation. Cut-off value in ELISA by CBE and OMP antigen were 1.134 and 1.217, respectively. By the ELISA, positive reaction rates to A pleuropneumoniae serotype 2 and 5 for CBE antigen were 38.8% and 18.8%, and for OMP antigen were 42.8% and 23.5% of the 400 samples, respectively.

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Load Distribution over Multipath for MPLS Networks (MPLS 네트워크에서의 다중 경로 부하 분산 방안)

  • 김세린;이미정
    • Proceedings of the Korean Information Science Society Conference
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    • 2001.04a
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    • pp.397-399
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    • 2001
  • IETF에서는 차세대 인터넷 기술인 MPLS를 도입한 망에서의 다중경로 라우팅 및 부하 분산 방식으로서 MPLS OMP(Optimized MultiPath)를 제안하였다. 그런데, MPLS OMP는 다중 경로 집합을 계산하고 이 집합에 속하는 경로들의 활용률이 동일해 지는 것을 목표로 부하를 골고루 분산하기 때문에 긴 경로와 짧은 경로가 동일하게 활용되어 대역폭을 낭비할 수 있다는 단점이 있다. 이에 본 논문에서는 좀 더 MPLS 네트워크 자원을 효과적으로 사용하는 다중 경로 라우팅 방식을 제안하였다. 제안한 다중 경로 라우팅 방식은 최단경로의 활용률이 낮을때는 최단경로를 사용하고, 최단경로의 활용률이 높아지면 좀 더 길지만 활용률이 낮은 대체 경로 집합을 계산하여 대체 경로 집합의 경로들 중 흡수와 활용률을 반영해 무작위로 한 경로를 선택한다. 또한, 링크가 낭비되는 것을 막기 위해 링크의 활용률이 클수록 더 짧은 경로에 의해서만 사용되도록 제한한다. 그리고 계산한 대체 경로 집합의 활용률이 임계치 이상인 경우에는 대체 경로 집합의 크기를 늘린다. 시뮬레이션을 통하여 제한하는 방식과 단순한 최단 경로 방식을 비교한 결과, 제안하는 방식의 셀 손실률이 낮고, 연결 수락률이 높음을 볼 수 있었다.

Study of the Compressed and Expanded Liquid Rubidium with the Optimized Nonlocal Model Potential

  • 강홍석;박성호
    • Bulletin of the Korean Chemical Society
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    • v.20 no.11
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    • pp.1288-1294
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    • 1999
  • We show that Shaw's optimized nonlocal model potential (OMP) in combination with the perturbative hyper-netted-chain equation for pair correlation functions can be successfully applied to predict pair structures of compressed and expanded liquid rubidium. For compressed rubidium, it is possible to apply the OMP to a state for which the model radius is even close to the Wigner-Seitz radius. In addition, our results are parallel to those from Chihara and Kahl's quantal hypernetted-chain equation in that it supports the uniform compression model up to 6.1 GPa. Calculation also shows that the pair structure is relatively insensitive to the choice of the exchange-correlation function for the electron liquid. Discussions are also given for compressed and expanded cesium.

Compressive sensing-based two-dimensional scattering-center extraction for incomplete RCS data

  • Bae, Ji-Hoon;Kim, Kyung-Tae
    • ETRI Journal
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    • v.42 no.6
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    • pp.815-826
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    • 2020
  • We propose a two-dimensional (2D) scattering-center-extraction (SCE) method using sparse recovery based on the compressive-sensing theory, even with data missing from the received radar cross-section (RCS) dataset. First, using the proposed method, we generate a 2D grid via adaptive discretization that has a considerably smaller size than a fully sampled fine grid. Subsequently, the coarse estimation of 2D scattering centers is performed using both the method of iteratively reweighted least square and a general peak-finding algorithm. Finally, the fine estimation of 2D scattering centers is performed using the orthogonal matching pursuit (OMP) procedure from an adaptively sampled Fourier dictionary. The measured RCS data, as well as simulation data using the point-scatterer model, are used to evaluate the 2D SCE accuracy of the proposed method. The results indicate that the proposed method can achieve higher SCE accuracy for an incomplete RCS dataset with missing data than that achieved by the conventional OMP, basis pursuit, smoothed L0, and existing discrete spectral estimation techniques.

Characterization of Vibrio vulnificus Isolated from Domestic Coastal Area (국내 연안에서 분리한 Vibrio vulnificus의 특성)

  • Park Geun-Tae;Park Min Jung;Jung Cho-Rok;Song Choon-Bok;Lee Jehee;Yeo In-Kyu;Jeon You Jin;Heo Moon-Soo
    • Journal of Life Science
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    • v.14 no.6 s.67
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    • pp.986-990
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    • 2004
  • Five strains of Vibrio vulnificus, which cause serious septicemia in human worldwidely, was isolated from marine environments of Korea costal area from May to July of 2002. The isolated strains were identified by API 20E kit and partial 16S rDNA sequence analysis. 16S rDNA sequence of the isolates showed $99\%$ similarity with V. vulnificus ATCC 27562. The proteins of V. vulnificus isolates were examined by analyzing patterns of the cell lysates and outer membrane proteins (OMP). The OMP separated from cell lysates showed the common protein band. Therefore OMP profiles might be useful for the identification of V. vulnificus sp.

Physicochemical Properties and the Origin of Summer Bottom Cold Waters in the Korea Strait (하계 대한해협 저층냉수의 물리.화학적인 특성 및 기원)

  • Kim, Il-Nam;Lee, Tong-Sup
    • Ocean and Polar Research
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    • v.26 no.4
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    • pp.595-606
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    • 2004
  • Hydrographic survey in the Korea Strait has long history that has begun in August 1917 at the Busan - Tsushima cross section, still continues to date. However, chemical properties of bottom cold water found exclusively in the western channel of the Korea Strait during summer did not receive much scientific attention. The aim of the study is to decipher the enigmatic origin of the Korea Strait Bottom Cold Water (KSBCW) in terms of chemical properties. The physicochemical properties of the KSBCW are extracted from the CREAHS II hydrographic data. OMP method was applied to analyze origin of the KSBCW quantitatively. The KSBCW is well defined by low temperature below $10^{\circ}C$. The cold waters exhibited the local presence near the coast at about 120m depth with a thickness of 20m to 30m. The cold water was characterized by relatively cold, saline and higher chemical concentrations than adjacent waters. The KSBCW seems to have different origin kom that of the coastal upwelled waters at the Ulgi-Gampo because it is saline, denser and contains considerably less dissolved oxygen than upwelled waters. The physicochemical properties are reported to have noticeable annual variations which suggest the complex origin of the KSBCW. OMP analysis show that the KSBCW is a mixture of three water types; TMW (24%), ESIW (36%) and ESPW (40%). Relationship between the KSBCW and the east Sea circulation is traced by mapping the water masses that have similar T, S and DO of KSBCW. The result showed that the KSBCW is most possibly an extension of southward flowing coastal intermediate waters. Front these results, we expect that the monitoring KSBCW will provide us valuable information about the East Sea circulation.