• 한국융합학회논문지
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• 제9권4호
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• pp.351-356
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• 2018

본 연구는 교양체육인 스포츠건강체력 수업에 참여하는 대학생의 웰니스 건강인식의 변화를 확인하기 위하여 D시 소재 대학교에 재학중인 168명을 대상으로 실시하였다. 스포츠건강체력 수업은 대학생의 집중적인 건강체력관리를 위해 1학기동안 진행되었다. 웰니스 건강인식은 한국인의 웰니스설문지(KWLS)를 이용하여 조사하였으며 수집된 자료는 연구목적에 따라 기술통계분석, 신뢰도분석, 이원변량분석을 실시하였다. 분석결과 대학생의 웰니스 건강인식은 성별과 시행(수업실시 전 후)간의 통계적으로 유의한 상호작용효과는 나타나지 않았으나(p>.05) 성별, 시행 각 요인간의 차이는 일부 하위영역에서 유의하게 나타났다(p<.05). 이러한 결과를 토대로 대학생의 웰니스 건강인식의 일부 요인이 스포츠건강체력 수업 전과 후에 변화하는 것을 확인하였다. 따라서 대학생의 웰니스 건강인식의 긍정적인 변화를 위한 해결책으로 각 대학에서 실시하는 다양한 교양체육 외에도 집중적인 건강체력관리를 목적으로 하는 수업을 대학생들에게 제공할 필요가 있는 것으로 사료된다.

• 상하수도학회지
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• 제28권4호
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• pp.473-478
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• 2014

Microalgae is known as one alternative energy source of the fossil fuel with the small size of $5{\sim}50{\mu}m$ and negative charge. Currently, the cost of microalgae recovery process take a large part, about 20 - 30% of total operating cost. Thus, the microalgae recovery method with low cost is needed. In this study, the optimum current for Scenedesmus dimorphus recovery process using electrocoagulation techniques was investigated. Under the electrical current, Al metal in anode electrode is oxidized to oxidation state of $Al^{3+}$. In the cathode electrode, the water electrolysis generated $OH^-$ which combine with $Al^{3+}$ to produce $Al(OH)_3$. This hydroxide acts as a coagulant to harvest microalgae. Before applying in 1.5 L capacity electrocoagulation reactor, Scenedesmus dimorphus was cultured in 20 L cylindrical reactor to concentration of 1 OD. The microalgae recovery efficiency of electrocoagulation reactor was evaluated under different current conditions from 0.1 ~ 0.3 A. The results show that, the fastest and highest recovery efficiency were achieved at the current or 0.3 A, which the highest energy efficiency was achieved at 0.15 A.

• 한국환경보건학회지
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• 제35권5호
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• pp.393-401
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• 2009

We isolated and identified a microorganism which was excellent for ammonia oxidation in the biological control of ammonia gas in odor producing materials from organic composting. The isolated strain was tested for growth characteristics and ammonia elimination efficiency under various conditions of temperature, pH, carbon concentration and ammonia concentration. The strain was isolated from a culture broth used in a $NO_2$ producing test with Griess-Ilosvay reagent. The results of 16S rRNA sequence from the isolated strain by using BLANST (Basic Local Alignment Search Tool) and confirming RDP (Ribosomal Database Project II) and ERRD (The European Ribosomal RNA Database) indicate that the strain is related to Delftia sp. UV-Spectrophotometer (Shimadzu, UVmini-1240) was used as a microbial growth test by measuring turbidity on OD660nm and ammonia concentration was measured by Spectrophotometer (HACH, DR-4000). The optimum growth culture conditions of the ammonia oxidizer Delftia sp. were $30^{\circ}C$, pH 7, glucose concentration 1.00% and $(NH_4)_2SO_4$ 0.5 g/l. Ammonia elimination efficiency was over 94% under the same conditions.

• 한국미생물·생명공학회지
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• 제44권4호
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• pp.497-503
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• 2016

전처리 폐글리세롤을 기질로 사용한 재조합 대장균 배양에서 트레할로스 생산성에 영향을 미치는 핵심 변수들을 도출하고 반응표면 분석법을 사용하여 트레할로스 생산을 극대화하기 위한 최적조건을 탐색하였다. $37^{\circ}C$에서 IPTG로 induction 하는 배양이 $27^{\circ}C$ 또는 induction 하지 않은 배양에 비하여 세포생장 및 트레할로스 생산성이 높았다. Box-Behnken design 실험계획법을 사용하여 배지중의 NaCl, 발리다마이신 및 IPTG의 농도를 최적화하였다. 통계분석결과 IPTG와 NaCl의 농도는 트레할로스 생산에 영향을 미쳤으나 발리다마신의 영향은 크지 않은 것으로 확인되었다. 등고선도 분석을 통해 298 mM NaCl이 첨가되고 0.1 mM의 IPTG로 induction되는 배양에서 가장 많은 양의 트레할로스가 생산되는 것으로 예측되었다. 최적화된 조건에서 생산 균주는 세포 밀도($OD_{600}$) $5.4{\pm}0.2$에서 $304{\pm}15mg/l$의 트레할로스를 생산하였다.

• 한국수산과학회지
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• 제50권5호
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• pp.547-552
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• 2017

The viral hemorrhagic septicemia virus (VHSV) has an extensive host range, and infects farmed and wild fish inhabiting both freshwater and marine ecosystems. Enzyme-linked immunosorbent assay (ELISA) is highly useful in diagnosing viral hemorrhagic septicemia. However, ELISA shows high, non-specific background reaction with fish antibodies. In this study, we optimized the antigen and antibody concentrations used for detecting specific antibodies in VHSV-infected olive flounder to reduce non-specific binding, and improve the sensitivity of ELISA. The results suggested that OD (optical Density) values were valid when ELISA was performed with $0.1{\mu}g/well$ of virus, involving blocking with blocking buffer (Roth, Roti-Block), 1:300-1:600 dilution with flounder antisera, and 1:1000 dilution with anti-flounder IgM and HRP-conjugated goat anti-mouse IgG for detecting the VHSV antibody in flounder sera. Furthermore, 11 different VHSV strains isolated in Korea from 2012 to 2016 were used to infect the fish. The results showed no correlation between viral pathogenicity and antibody production. This research is a basic study on the application of antibody detection in the diagnosis of viral hemorrhagic septicemia in the olive flounder.

• Membrane and Water Treatment
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• 제2권1호
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• pp.25-37
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• 2011

PES UF membranes containing silver were prepared to impart antibacterial properties for waste water treatment. Asymmetric membranes for antibacterial application were prepared from polyethersulfone (PES) and silver nitrate ($AgNO_3$) (PES/$AgNO_3$=15/2 by weight) solution in N-Methyl-2-pyrrolidone (NMP) via simple wet phase inversion technique. These membranes were characterized by polyvinylpyrrolidone (PVP) and polyethylene glycol (PEG) of different molecular weights (1000 ppm in water) at room temperature and on operating pressure of 5 bars. It was observed that the water flux of PES-$AgNO_3$ membrane is slightly lower than virgin PES but still increased linearly with the increment of pressure applied. The morphology of the resulting membranes was examined using Field-Emission Scanning Electron Microscope (FESEM) coupled with Energy Dispersive Spectroscopy (EDS). Elemental analysis using EDS proved that silver is successfully loaded on the membrane surfaces. Due to the success of loading silver on membrane surfaces, antibacterial activities were evaluated via agar diffusion method against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) culture. By incorporating 2 wt% of silver nitrate, PES-$AgNO_3$ showed significant inhibition ring on both E.coli and S.aureus. Filtration of E.coli solution (OD 0.31) showed satisfactory rejection data with ~100% inhibition growth after 24 hours incubation at $37^{\circ}C$. Resultant membranes also exhibit better tensile strength (compared to virgin PES) up to 71% may be due to the suggested interactions. The residual silver during fabrication was measured using ICP-MS and result showed that the residual silver content of PES-$AgNO_3$ membrane was only ~1% of the original silver added in the polymer solution. These studies have shown that PES-$AgNO_3$ UF membranes are potential in improving the filtration in water treatment.

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2555-2559
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• 2015

Purpose: To investigate effects of the TESTIN (TES) gene on proliferation and migration of highly metastatic nasopharyngeal carcinoma cell line 5-8F and the related mechanisms. Materials and Methods: The target gene of human nasopharyngeal carcinoma cell line 5-8F was amplified by PCR and cloned into the empty plasmid pEGFP-N1 to construct a eukaryotic expression vector pEGFP-N1-TES. This was then transfected into 5-8F cells. MTT assays, flow cytometry and scratch wound tests were used to detect the proliferation and migration of transfected 5-8F cells. Results: A cell model with stable and high expression of TES gene was successfully established. MTT assays showed that the OD value of 5-8F/TES cells was markedly lower than that of 5-8F/GFP cells and 5-8F cells (p<0.05). Flow cytometry showed that the apoptosis rate of 5-8F/TES cells was prominently increased compared with 5-8F/GFP cells and 5-8F cells (p<0.05). In vitro scratch wound assays showed that, the width of the wound area of 5-8F/TES cells narrowed slightly, while the width of the wound area of 5-8F/ GFP cells and 5-8F cells narrowed sharply, suggesting that the TES overexpression could inhibit the migration ability. Conclusions: TES gene expression remarkably inhibits the proliferation of human nasopharyngeal carcinoma cell line 5-8F and reduces its migration in vitro. Thus, it may be a potential tumor suppressor gene for nasopharyngeal carcinoma.

• Nutrition Research and Practice
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• 제7권5호
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• pp.400-408
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• 2013

Obesity may be the consequence of various environmental or genetic factors, which may be highly correlated with each other. We aimed to examine whether grandmaternal and maternal obesity and environmental risk factors are related to obesity in daughters. Daughters (n = 182) recruited from female students, their mothers (n = 147) and their grandmothers (n = 67) were included in this study. Multivariable logistic regression was used to analyze the association between the daughter's obesity and maternal, grandmaternal, and environmental factors. Maternal heights of 161-175cm (OD: 8.48, 95% CI: 3.61-19.93) and 156-160 cm (2.37, 1.14-4.91) showed positive associations with a higher height of daughter, compared to those of 149-155 cm. Mothers receiving a university or a higher education had a significant OR (3.82, 1.27-11.50) for a higher height of daughter compared to those having a low education (elementary school). Mother having the heaviest weight at current time (59-80 kg, 3.78, 1.73-8.28) and the heaviest weight at 20 years of age (51-65 kg, 3.17, 1.53-6.55) had significant associations with a higher height of daughters, compared to those having the lightest weight at the same times. There was no association between the height, weight, and BMI of daughters and the characteristics and education of her grandmothers. In conclusion, although genetic factors appear to influence the daughter's height more than environmental factors, the daughter's weight appears to be more strongly associated with individual factors than the genetic factors.

• 한국통신학회논문지
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• 제18권8호
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• pp.1169-1175
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• 1993

레이다의 추적 및 탐색으로 부터 비행 물체를 보호하기 위한 목적으로, 알루미늄 합금표면에 페라이트-탄화규소 복합물인 마이크로파 흡수층을 플라즈마 용사방식으로 제작하였다. 본 논문에서는 페라이트-탄화규소층(I) 제조시 사용했던 탄화규소 입자의 평균크기인 34[rm]대신에15[rm]가 사용되었으며, 플라즈마 용사변수들 중에서 분말의 공급비율은 70[Kg/h]대신에 50[Kg/h] 그리고, 용사거리는 80[mm[ 대신에 100[mm]가 사용 되었다. X-band(8~12.4(GHz)레이다용 페라이트-탄화규소 전자파 흡수체를 실험적으로 설계하고 시험제작하여 전기적 특성을 평가한 결과, -lOdB의 반사량을 허용한도로 했을 때 약 2.8%의 대역폭이 얻어졌으며, 최대 흡수두께는 0.5(mm)로 매우 양호한 박층형 전자파 흡수체가 얻어졌다.

• Bulletin of the Korean Chemical Society
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• 제28권12호
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• pp.2377-2381
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• 2007

Solvolyses of 3,4-dimethoxybenzenesulfonyl chloride (DSC) in water, D2O, CH3OD, and in aqueous binary mixtures of acetone, acetonitrile, 1,4-dioxane, ethanol, methanol, and 2,2,2-trifluoroethanol (TFE) have been investigated at 25.0 oC. Kinetic solvent isotope effects (KSIE) in water and in methanol and product selectivities in alcohol-water mixtures are also reported. The Grunwald-Winstein plot of first-order rate constants for the solvolyic reaction of DSC with YCl shows marked dispersions into separated lines for various aqueous mixtures. With use of the extended Grunwald-Winstein equation, the l and m values obtained are 1.12 and 0.58 respectively for the solvolyses of DSC. The relatively large magnitude of l is consistent with substantial nucleophilic solvent assistance. From Grunwald-Winstein plots the rate data are dissected approximately into contributions from two competing reaction channels. This interpretation is supported for alcohol-water mixtures by the trends of product selectivities, which show a maximum for ethanol-water mixtures. From the KSIE of 1.45 in methanol, it is proposed that the reaction channel favored in methanolwater mixtures and in all less polar media is general-base catalysed and/or is possibly (but less likely) an addition-elimination pathway. Also, the KISE value of 1.35 for DSC in water is expected for SN2-SN1 processes, with minimal general base catalysis, and this mechanism is proposed for solvolyses in the most polar media.