• Restorative Dentistry and Endodontics
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• 제31권6호
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• pp.437-444
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• 2006

본 연구에서는 법랑모세포 분화와 법랑질 형성에 연관이 있는 OD314 일명 Apin protein의 기능을 밝힐 목적으로, in-situ hybridization에 의한 OD314 mRNA 발현과 법랑모세포 세포주에서 OD314와 enamel matrix protein의 발현, 그리고 OD314 유전자를 과발현/억제시킬 수 있는 construct를 제작한 후 법랑질 형성 중에 OD314의 기능을 알아보고자 RT-PCR를 시행하여 다음과 같은 결과를 얻었다. 1. OD314 mRNA는 발생중인 상아모세포보다 법랑모세포에서 강하게 발현되었다. 2. Tuftelin은 석회화 결정이 형성되는 14일까지 발현이 지속되고, 그 이후부터 점차 감소하였다. Amelogenin과 enamelin은 7일부터 그 발현이 점점 감소하였다. 3. U6-OD314 siRNA construct를 이용하여 transfection한 법랑모세포 세포주는 OD314와 tuftelin, MMP20 mRNA 발현이 감소하였으며, CMV-OD3l4를 transfection하여 OD314의 과발현을 유도한 경우에는 OD3l4와 MMP20 mRNA의 발현이 뚜렷이 증대되었다. 이 결과는 OD314가 법랑모세포의 분화와 법랑질의 형성 그리고 석회화 과정에 중요한 역할을 하는 새로운 인자임을 시사한다.

• Imaging Science in Dentistry
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• 제53권2호
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• pp.117-126
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• 2023

Purpose: Odontomas represent a common clinical entity among odontogenic tumors, but are not well-addressed in the Vietnamese population. The present study aimed to determine the clinical and preclinical characteristics of odontomas and associated factors in the Vietnamese population. Materials and Methods: This retrospective study retrieved data from histopathological diagnoses from 2 central hospitals of Odonto-Stomatology in Ho Chi Minh City, Vietnam during 2004-2017. The odontomas were classified as complex (CxOD) or compound (CpOD) subtypes. The epidemiological, clinical, and radiological characteristics of the odontomas, stratified by subtype and sex, were obtained and analyzed. Results: Ninety cases, consisting of 46 CxODs and 44 CpODs, were included. The average age of patients was 32.4 (±20.2) years. The patients with CxOD were older than those with CpOD (P<0.05). Clinically, 67% of patients showed an intraoral bone expansion. Approximately 60% of patients with CxOD exhibited a painful symptom, about 3-fold more than those with CpOD (P<0.05), whereas almost all patients with CpOD exhibited perturbations of dentition, unlike those with CxOD (P<0.05). Radiologically, CxOD was characterized by a larger dimension than CpOD in both sexes (P<0.05), and CpOD induced complications in adjacent teeth more often than CxOD (P<0.05). The development of odontoma with advancing age differed significantly in odontoma subtypes related to their pathological origins, and between the sexes, resulting from different physiological states. Conclusion: The findings of this study highlight the value of clinical and radiological features of odontomas and their associated factors for the early diagnosis and adequate treatment of younger patients.

• 대한기계학회논문집B
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• 제25권7호
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• pp.981-988
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• 2001

Characteristics of evaporation heat transfer in 6.2 and 5.1mm OD micro-fin tubes were investigated in the present study. The data were taken at evaporation temperatures of -5$^{\circ}C$ and 5$^{\circ}C$ and heat fluxes 5kW/$m^2$ to 10kW/$m^2$. Mass flux was consequently maintained at 210, 300 and 410kg/$m^2$s for the 6.2mm OD tube and 465, 500 and 600kg/$m^2$s for the 5.1mm OD tube. The effects of heat flux, mass flux, and outer diameter on the heat transfer coefficient are explored in the present study. The data showed that the evaporation heat transfer coefficient for the 6.2mm OD tube was averagly higher by 16% than that for a 7.0mm OD tube, while the 5.1mm OD tube had approximately 30% higher value than the 6.2mm OD tube.

• 한국응용과학기술학회지
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• 제38권3호
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• pp.824-831
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• 2021

안전한 화장품용 방부제에 대한 연구를 위하여 1, 2-octanediol (OD)에 galactose 한 분자가 결합된 1, 2-octanediol galactoside (OD-gal)의 합성을 시도하였다. 이를 위하여, 재조합 대장균의 β-galactosidase (β-gal)를 이용하여 transgalactosylation 반응을 수행하였으며, OD-gal 합성을 확인하기 위하여 mass spectrometry 분석과 NMR (¹H- and ¹³C-) spectroscopy 분석을 실시하였다. 합성반응물에서 m/z=331.1732의 sodium adduct ion 형태로 OD-gal 분자의 합성을 확인하였고, 정제된 OD-gal의 NMR 분석을 통하여 OD-gal의 ¹H NMR 스펙트럼에서 OD에 갈락토실화가 되었음을 보여주는 다양한 피크를 확인하였다. ¹H NMR 스펙트럼의 다운필드인 δ_H 4.39 ppm과 δ_H 3.98~3.55 ppm에서 나타나는 다양한 피크들은 이들이 OD에 갈락토실화가 되었다는 것을 잘 암시하고 있으며, 또한 ¹H NMR 스펙트럼의 업필드에서 나타나는 δ_H 1.52~1.26 ppm과 0.89 ppm의 피크는 OD의 CH₂ 와 CH₃ 작용기로 부터 나타나는 피크로써 OD가 본 물질에 존재함을 알 수 있었다. ¹³C NMR 스펙트럼에서는 OD-gal의 알파-아노머와 베타-아노머의 구조에서 기인하는 총 24개의 탄소피크가 나타났고, 각 아노머 마다 14개의 탄소가 존재하는데 이중 δ_c 31.4, 29.0, 22.3 그리고 13.7 ppm에 보이는 OD 4개의 탄소는 지방족 사슬의 끝부분에 해당하며 화학적 구조의 유사성으로 인하여 탄소 피크가 겹쳐서 나타난 것으로 보인다. 따라서 총 28개의 탄소 피크 중 24개가 나타났다. 마지막으로, 합성된 OD-gal의 β-gal을 이용한 가수분해 반응을 통하여 OD-gal에 gal이 결합되어 있다는 것을 확인하였다. 이러한 결과를 바탕으로 세포독성이 감소된 첨가물 개발을 기대하고 있으며, 추가적인 후속연구를 진행할 예정이다.

• Restorative Dentistry and Endodontics
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• 제29권4호
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• pp.399-408
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• 2004

Odontoblasts are responsible for the formation and maintenance of dentin. They are known to synthesize unique gene products including dentin sialophosphoprotein (DSPP). Another unique genes of the cells remain unclear. OD314 was isolated from the odontoblasts/pulp cells of rats and partially characterized as an odontoblast-enriched gene (Dey et al., 2001). This study aimed to elucidate the biological function of OD314, relating to odontoblast differentiation and dentinogenesis. After determining the open reading frame (ORP) of OD314 by transient transfection analysis using green fluorescent protein (GPP) expression vector, mRNA in-situ hybridization, immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR) and western analysis were performed. The results were as follows: 1. In in-situ hybridization, OD314 mRNAs were expressed in odontoblasts of developing coronal and root pulp. 2. OD314 was a novel protein encoding 154 amino acids, and the protein was mainly expressed in cytoplasm by transient transfection analysis. 3. Mineralized nodules were associated with multilayer cell nodules in the culture of human dental pulp cells and first detected from day 21 using alizarin-red S staining. 4. In RT-PCR analysis, OD314, osteocalcin (OC) and DSPP strongly expressed throughout 28 days of culture. Whereas, osteonectin (ON) mRNA expression stayed low up to day 14, and then gradually decreased from day 21. 5. Western blots showed an approximately 17 kDa band. OD314 protein was expressed from the start of culture and then increased greatly from day 21. In conclusion, OD314 is considered as an odontoblast-enriched gene and may play important roles in odontoblast differentiation and dentin mineralization.

• 한국ITS학회 논문지
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• 제17권6호
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• pp.40-53
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• 2018

교통카드자료에서 철도이용승객이동은 단말기ID 순서로 나타난다. 최초 단말기ID는 진입역사 Tag-In노선, 최종 단말기ID는 진출역사 Tag-Out노선, 중간 단말기ID는 환승역사 Tag노선정보를 각각 포함한다. 과거 3개 공사기관(서울교통공사, 인천교통공사, 한국철도공사)만 참여하던 수도권도시철도는 최초 및 최종 단말기ID로 표현된 OD만 존재했다. 최근 (주)신분당선, (주)우이-신설경전철 등 민자기관진입으로 진입환승노선 단말기ID와 진출환승노선 단말기ID가 포함된 Chain OD가 보편화되었다. Chain OD를 통한 정확한 경로구축과정은 수도권철도운송기관의 수입금배분의 기초자료로서 매우 중요한 위치를 차지하고 있다. 따라서 지하철네트워크에서 경로탐색은 Chain OD에 대한 최적경로탐색의 문제로 전환되어 새로운 해법이 요구된다. 본 연구는 철도네트워크에서 단말기 Chain OD의 최초 단말기ID와 최종 단말기ID 간의 최적경로탐색기법을 제안하는 것이다. 이때 Chain OD에 민자노선환승 TagIn/Out를 반영하여 최적경로를 탐색하는 방안을 강구한다. 이를 위해 링크표지로 구축된 3가지 경로탐색기법( 1) 노드 - 링크, 2) 링크 -링크, 3) 링크 -노드 )을 순차적으로 적용하는 방안을 제안한다. 가산성경로비용을 토대로 제안된 기법이 최적조건을 만족함을 증명한다.

• 한국융합학회논문지
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• 제10권12호
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• pp.315-325
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• 2019

본 연구의 목적은 누구나 쉽게 지하철 OD 이동패턴 정보를 분석할 수 있는 데이터마트를 설계하고 구축하는 것이다. 서울 열린데이터광장에서 받은 2017년도 데이터를 원천 데이터로 이용하였다. 다차원모델을 설계하였고, 가우시안 혼합 군집분석과 Tableau를 이용한 다차원 분석을 실시하였다. 흥미로운 사실은 교외지역과 서울 간 이동은 전체 이동량의 23%에 해당하며, 수원역의 이용객은 서울보다 교외로의 이동이 매우 크며, 반면 판교역은 이동량의 대부분이 서울로의 이동이다. 가우시안 혼합 군집결과 8개의 OD구간 군집을 발견하였고, 구간 거리와 승객 수에 의해 각 군집의 특징을 네이밍하였다.

• Korean Circulation Journal
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• 제54권5호
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• pp.270-287
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• 2024

Background and Objectives: Blood pressure (BP) measurement using an auscultatory sphygmomanometer is recommended for diagnosing hypertension in children. As mercury sphygmomanometers (MSs) are banned owing to environmental concerns, it is crucial to determine the accuracy of mercury-free sphygmomanometers to replace them. We analyzed the accuracy of these devices to guide the National Survey selection. Methods: BP was measured thrice each with MS, auscultatory device (AD), and oscillometric device (OD) in 104 participants aged 10-18 using the National Survey data. The difference in BP was defined as the difference between MS and other devices. The BP differences, correlations, and influencing factors were analyzed. The frequencies of hypertension were also compared. Results: Systolic BP (SBP) and diastolic BP (DBP) differences between MS and AD were 0.88±3.36 mmHg and 0.63±3.95 mmHg, and those between MS and OD were 0.43±5.83 mmHg and 4.57±6.89 mmHg, respectively. The absolute error of <10 mmHg for DBP between MS and OD was 76%. The concordance correlation coefficient between MS and AD was 0.94 for SBP and 0.90 for DBP, and 0.81 and 0.67, respectively for MS and OD. Arm circumference negatively correlated with BP differences except for SBP between the MS and OD. The frequency of hypertension was not different between MS and AD but was underestimated by OD. Conclusions: AD correlated well with MS, while OD did not, especially for DBP. The superiority of AD over OD suggests AD as a possible alternative for MS in the National Survey.

• Journal of Astronomy and Space Sciences
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• 제35권4호
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• pp.295-308
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• 2018

In this study, orbit determination (OD) simulation for the Korea Pathfinder Lunar Orbiter (KPLO) was accomplished for investigation of the observational arc-length effect using a sequential estimation algorithm. A lunar polar orbit located at 100 km altitude and $90^{\circ}$ inclination was mainly considered for the KPLO mission operation phase. For measurement simulation and OD for KPLO, the Analytical Graphics Inc. Systems Tool Kit 11 and Orbit Determination Tool Kit 6 software were utilized. Three deep-space ground stations, including two deep space network (DSN) antennas and the Korea Deep Space Antenna, were configured for the OD simulation. To investigate the arc-length effect on OD, 60-hr, 48-hr, 24-hr, and 12-hr tracking data were prepared. Position uncertainty by error covariance and orbit overlap precision were used for OD performance evaluation. Additionally, orbit prediction (OP) accuracy was also assessed by the position difference between the estimated and true orbits. Finally, we concluded that the 48-hr-based OD strategy is suitable for effective flight dynamics operation of KPLO. This work suggests a useful guideline for the OD strategy of KPLO mission planning and operation during the nominal lunar orbits phase.

• 한국어병학회지
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• 제37권1호
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• pp.9-15
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• 2024

An enzyme-linked immunosorbent assay (ELISA) with two Novirhabdovirus antigens (viral hemorrhagic septicemia virus, VHSV and infectious hematopoietic necrosis virus, IHNV) was used to detect specific antibodies against VHSV from olive flounder (Paralichthys olivaceus) sera. In ELISA plates with VHSV culture supernatants (VHSV-Ag plate), optical density (OD) values for sera from olive flounder with VHS history (VHS sera) ranged from 0.64±0.36, and those of sera from fish without VHS history (non-VHS sera) ranged from 0.26±0.26. In IHNV-Ag plate, the OD values (0.43±0.28) for VHS sera were quite low compared to those in VHSV-Ag plates, while the OD values for non-VHS sera were almost similar. When the OD values for each serum were calculated by subtracting the OD values in the IHNV-Ag plate from those in the VHSV-Ag plate, the corrected OD values were significantly different between VHS sera and non-VHS sera. The results were completely in line with fish histories of VHS epizootics. It was considered that the corrected OD values may represent the true values recognized by VHSV-specific antibodies.