• Title/Summary/Keyword: OCT-4

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Lysophosphatidic acid improves development of porcine somatic cell nuclear transfer embryos

  • Ling Sun;Tao Lin;Jae Eun Lee;So Yeon Kim;Ying Bai;Dong Il Jin
    • Journal of Animal Science and Technology
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    • v.66 no.4
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    • pp.726-739
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    • 2024
  • This study was conducted to investigate whether lysophosphatidic acid (LPA) could improve the development of porcine somatic cell nuclear transfer (SCNT) embryos. Porcine SCNT-derived embryos were cultured in chemically defined polyvinyl alcohol (PVA)-based porcine zygote medium (PZM)-4 without or with LPA, and the development, cell proliferation potential, apoptosis, and expression levels of pluripotent markers were evaluated. LPA significantly increased the rates of cleavage and blastocyst formation compared to those seen in the LPA un-treatment (control) group. The expression levels of embryonic development-related genes (IGF2R, PCNA and CDH1) were higher (p < 0.05) in the LPA treatment group than in the control group. LPA significantly increased the numbers of total, inner cell mass and EdU (5-ethynyl-2'-deoxyuridine)-positive cells in porcine SCNT blastocysts compared to those seen in the control group. TUNEL assay showed that LPA significantly reduced the apoptosis rate in porcine SCNT-derived embryos; this was confirmed by decreases (p < 0.05) in the expression levels of pro-apoptotic genes, BAX and CASP3, and an increase (p < 0.05) in the expression level of the anti-apoptotic gene, BCL2L1. In addition, LPA significantly increased Oct4 expression at the gene and protein levels. Together, our data suggest that LPA improves the quality and development of porcine SCNT-derived embryos by reducing apoptosis and enhancing cell proliferation and pluripotency.

Effects of the Hippo Signaling Pathway in Human Gastric Cancer

  • Zhou, Guang-Xi;Li, Xiao-Yu;Zhang, Qi;Zhao, Kun;Zhang, Cui-Ping;Xue, Chang-Hu;Yang, Kun;Tian, Zi-Bin
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.9
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    • pp.5199-5205
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    • 2013
  • Background/Aim: The Hippo signaling pathway is a newly discovered and conserved signaling cascade, which regulates organ size control by governing cell proliferation and apoptosis. This study aimed to investigate its effects in human gastric cancer. Methods: Tumor tissues (n=60), adjacent non-tumor tissues (n=60) and normal tissues (n=60) were obtained from the same patients with primary gastric cancer (GC). In addition, 70 samples of chronic atrophic gastritis (CAG) tissues were obtained from patients with intestinal metaplasia (IM) by endoscopic biopsy. Hippo signaling molecules, including Mst1, Lats1, YAP1, TAZ, TEAD1, Oct4 and CDX2, were determined by quantitative polymerase chain reaction (qPCR). Protein expression of Mst1, Lats1, YAP1, TEAD1 and CDX2 was assessed by immunohistochemistry and Western blotting. Results: Mst1, Lats1 and Oct4 mRNA expression showed an increasing tendency from GC tissues to normal gastric tissues, while the mRNA expression of YAP1, TAZ and TEAD1 was up-regulated (all P<0.01). Mst1 and Lats1 protein expression presented a similar trend with their mRNA expression. In addition, YAP1 and TEAD1 protein expression in GC was significantly higher than in the other groups (all P<0.01). CDX2 mRNA and protein expression in the CAG group were higher than in the other groups (all P<0.01). In GC, mRNA expression of Mst1, Lats1, Oct4, YAP1, TAZ, TEAD1 and CDX2 had a close correlation with lymphatic metastasis and tumor TNM stage (all P<0.01). Furthermore, protein expression of Mst1, Lats1, YAP1, TAZ, TEAD1 and CDX2 had a close correlation between each other (P<0.05). Conclusion: The Hippo signaling pathway is involved in the development, progression and metastasis of human gastric cancer. Therefore, manipulation of Hippo signaling molecules may be a potential therapeutic strategy for gastric cancer.

An Analysis on Factors Related to Health Management & Disease Awareness of Industrial Workers (산업장 근로자들의 건강관리 및 질병에 대한 인식과 관련요인)

  • 전봉천;남철현
    • Korean Journal of Health Education and Promotion
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    • v.13 no.2
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    • pp.1-23
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    • 1996
  • This study was conducted to investigate health management and health awareness of industrial workers and use the information for the development of health education program and the guidline of health management for industrial workers. The survey was carried out on 1,200 workers who were selected from Incheon, Ulsan, Pohang, Masan, Changwon city from Oct. 2 to Oct. 31. 1995. The main results are summarized as follows. 1. With regard to health behavior after regular physical examination, 48.3% of subjests answered "consulting with medical doctors or health workers" and 7.9% answered "non action anything", 2. As for need of environmental control of work, of the total subjects, 96.1% answered "need". 3. As for participation of health education, 61.2% of the subjects were positive, which was higher in male, in higher age groups, in middle school graduates, in healthy group by self assessment than other groups respectively. 4. As for knowledge level of the disease, the average score was $4,97{\pm}1.41$ point to 7 point full mark (71 point to 100 point full mark), the score level was higher in male higher economic, in office workers, in higher education groups, in working period longer groups, in healthier groups, in higher age groups than other groups respectively. 5. As for attitude level of the disease, the average score was $4,70{\pm}1.25$ point to 7 point full mark (67.1 point to 100 point full mark), which was lower, in higher age group, in longer education level, in healthier groups in longer working period than other groups respectively, while the score was higher in male, in day-time workers, in urban residence than other groups respectively.han other groups respectively.

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A Study of Educational Satisfaction of the Students in Health Section and None Health Section based on 'D' college (보건계열 학생과 비보건계열 학생의 교육만족도 조사 - D대학을 중심으로 -)

  • Park, Myung-Ho;Lee, Hee-Kyung;Kim, Sang-Yeoun;Kwon, Sun-Il;Jun, Sung-Hee
    • Journal of Technologic Dentistry
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    • v.33 no.4
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    • pp.361-368
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    • 2011
  • Purpose: This study is to analyze the curriculum satisfaction and to provide basic information for effective school management of Dept. of Health section. Methods: This survey, modified questionnaires based on Hee-Joo Moon's(2005), is accordance with undergraduate students and shows very high credibility upper than Cronbach's Alpha. 1,500 questionnaires are distributed(01. Oct. 2010~30. Oct. 2010) but only 1,213(80.8%) are returned and, except for 14 insincerely answered, 1,199(79.9%) are used. Results: The results of the study are following. The population characteristic of this survey is that 445(18.1%) are male, 739(61.9%) are female, 595(49.6%) are in health section, and 604(50.4%) are in none health section. 628(53%) chose getting jobs as their motivations for school choice and 727(60.8%) chose getting jobs and career guidance as theirs. The satisfaction of male students was higher than that of female students based on the comparative analysis of the curriculum satisfaction. The satisfaction of the students in none health section was higher than that of those in health section based on the comparative analysis of the curriculum satisfaction. This survey indicates that once the satisfaction with the major courses and career guidance increase, the overall satisfaction grows. Conclusion: The keys to improve the curriculum satisfaction including students' in department of dental technology are to provide and develop differentiated curriculums and support programs that meet students' expectations. In addition, the customized curriculums based on the level and aptitudes of female students are needed.

Effect of Mesh Size of Net on Biomass Estimation of Acartia steueri (Copepoda: Calanoida) (네트 망목 크기가 Acartia steueri (Copepoda: Calanoida)의 생체량 추정에 미치는 영향)

  • Kang Hyung Ku;Kang Yong Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.4
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    • pp.445-450
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    • 2002
  • A series of 29 sampling with a 330 ${\mu}$m and a 64 ${\mu}$m mesh size of nets was conducted at a fixed station in Ilkwang Bay, southeast cost of Korea, from Oct, 2, 1991 to Oct. 10, 1992, to investigate the effects of mesh size of nets on biomass estimation of copepod Acartia steueri. The catch of copepodite and nauplius stages of A. steueli taken by two nets with different mesh size was different, showing that all developmental stages of A. steueri were retained on the 64 ${\mu}$m mesh net, but only $\geq$stage 4 copepodite were caught by the 330 ${\mu}$m mesh net. Abundance and biomass in each developmental stage estimated with the 64 ${\mu}$m mesh net were significantly higher than those of the 330 ${\mu}$m mesh net, except for adult female and stage 5 copepodite in female. The body length as well as the body width is likely to affect the catch of the nets. The mean biomass of A. steueli estimated with the traditional 330 ${\mu}$m net was 2.8 times lower than the value obtained with the 64 ${\mu}$m mesh net. However, the seasonal patterns of the biomass were comparable. These results suggest that accurate sampling strategr of the entire copepods assemblage including nauplii and copepodites are essential when estimating the abundance and biomass of copepods for the better understanding of the role of copepods in marine ecosystem.

Variation in Nitrate Contamination of Shallow Groundwater in a Farmland in Gyeonggi-do, Korea (경기도 지역 농경지의 천부 지하수 내 질산염 오염특성과 변화)

  • Lee, Eun-Jae;Woo, Nam-Chil;Lee, Byung-Sun;Kim, Yang-Bin
    • Economic and Environmental Geology
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    • v.41 no.4
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    • pp.393-403
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    • 2008
  • Hydrogeochemistry of groundwater was studied in order to identify the influence of cow manure, distributed to a farmland as organic fertilizer, on nitrate concentrations in shallow groundwater and its spatio-temporal variations. From monitoring wells, water levels were measured using automatic data loggers, and water samples collected and analyzed in Feb., April, June and Oct. 2007. The average electric conductivity and concentration of nitrate in the groundwater show the highest levels in April and decline in subsequent sampling times. Decreases in dissolved oxygen(DO) and nitrate concentrations from April to Oct. and corresponding increases in $HCO_3$ concentrations indicate denitrification processes by microorganisms. Spatial variation of nitrate concentration appeared to be affected by the redox conditions of groundwater controlled by geochemical reactions of Mn, Fe and DOC contents.

Preparation and Release Behavior of Methoxy poly(ethylene glycol)- poly(L-lactide-co-glycolide) Wafer Containing Albumin (알부민을 함유한 메톡시 폴리(에틸렌 글리콜)- 폴리(L-락타이드-co-글리콜라이드) 웨이퍼의 제조 및 방출거동)

  • 서광수;김문석;김경자;조선행;이해방;강길선
    • Polymer(Korea)
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    • v.28 no.4
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    • pp.328-334
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    • 2004
  • A series of methoxy poly(ethylene glycol) (MPEG)-poly(L-lactide-co-glycolide) (PLGA) diblock copolymers were synthesized by ring-opening polymerization of L-lactide and glycolide with carbitol (134 g/mole) or different molecular weights of MPEG (550, 2000, and 5000 g/mole) as an initiator in presence of Sn(Oct)$_2$. The properties of diblock copolymers were characterized by using $^1$H-NMR, GPC, and XRD. After uniform mixing of block copolymers and 1% albumin bovine-fluorescein isothiocyanate(FITC-BSA) with a freeze miller, the wafers loaded FITC-BSA were fabricated by using a mold with a dimensions of 3 mm${\times}$1mm diameter. The release profiles of FITC-BSA and the pH changes of wafer were examined using pH 7.4 PBS for 30 days at 37$^{\circ}C$. The release profiles of albumin showed fast initial burst as the molecular weights of MPEG increased. As a result of this study, the release behavior of BSA was controlled with introducing MPEG in the block copolymers.

Effects of seeding dates on dry matter yield and feed values of whole-crop barley cultivated in a paddy field

  • Lee, Sang Moo;Kim, Eun Joong
    • Journal of Animal Science and Technology
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    • v.61 no.5
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    • pp.278-284
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    • 2019
  • This study was carried out to investigate the effects of seeding dates on the yield and feed value of whole-crop barley cultivated in paddy soil. The field experiment was conceived as a randomized block design performed in triplicate with seeding dates of Oct. 19 (T1 as treatment 1), Oct. 26 (T2 as treatment 2), Nov. 2 (T3 as treatment 3), Nov. 9 (T4 as treatment 4), and Nov. 16 (T5 as treatment 5) as treatments. The barley grown in all treatments was harvested on May 21 of the following year. Plant length, fresh yield, dry matter yield, and total digestible nutrient (TDN) yield were higher (p < 0.05) in barley with early seeding dates, whereas crude protein and ether extract were higher (p < 0.05) with late seeding dates. Crude fiber and neutral detergent fiber were highest in the T3 treatment and lowest in the T1 treatment. There was no significant difference between acid detergent fiber and TDN among the different seeding dates. Total mineral contents were higher in the order T1 > T2 > T3 > T4 > T5 (p < 0.05), whereas the total amino acid content increased significantly (p < 0.05) with a delay in seeding date. Free sugar contents (fructose, glucose, and sucrose) were higher with early seeding dates (p < 0.05). Collectively, the results obtained in this study indicate that it is favorable to seed soon after harvesting rice to increase dry matter and TDN yields and mineral and free sugar contents of whole-crop barley in the midlands of Korea.

Recognition of Environmentally-friendly Agricultural Products for School Foodservice of Nutrition Teachers and Parents in 2018 at Seongnam in Gyeonggi province (성남지역 학교 영양(교)사와 학부모의 친환경농산물에 대한 인지도)

  • Kwon, Jisoo;Cho, Wookyoun
    • Korean Journal of Community Nutrition
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    • v.24 no.4
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    • pp.290-299
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    • 2019
  • Objectives: This study examined the nutrition teachers' and parents' recognition of environmentally-friendly agricultural products (EAPs) used in school foodservice. Methods: A questionnaire survey was given to 128 school foodservice nutrition teachers in Seongnam and 189 parents from Oct. 16 to Oct. 31, 2018 at Seongnam in Gyeonggi province. The survey included information on the recognition, satisfaction, and improvement of EAPs, and the results of the two groups were compared. Results: A comparison of the recognition of EAPs showed that nutrition teachers knew more about the EAPs and local government support in school foodservice than the parents. On the other hand, the parents were more aware than the nutrition teachers in that children have a higher affinity for EAPs than for general agricultural products in the school foodservice. A comparison of the level of satisfaction with the EAPs by nutrition teachers and parents revealed the nutrition teachers to be significantly more satisfied than parents in terms of the color, taste and nutrition of EAPs. Among the items that should be provided with EAPs, more than 50% of each group of nutrition teachers and parents answered that vegetables must be provided first. Some 70.9% of nutrition teachers and 84.5% of parents were aware of the certification standards of EAPs. The nutrition teachers had showed a slightly higher score than the parents in the certification system (3.51 vs. 3.25). In terms of improving the EAPs, 36.2% of nutrition teachers answered a reasonable price preferentially, whereas 56.4% of parents answered maintaining quality. In the expected effects of using EAPs, 57.9% of nutrition teachers answered an improvement of parents' satisfaction on the school foodservice. On the other hand, 38.0% of parents answered an improvement of children' satisfaction on school foodservice. Conclusions: Nutrition teachers and parents need to be educated on the certification systems that would enhance the trust in EAPs.

Acceleration of Mesenchymal-to-Epithelial Transition (MET) during Direct Reprogramming Using Natural Compounds

  • Seo, Ji-Hye;Jang, Si Won;Jeon, Young-Joo;Eun, So Young;Hong, Yean Ju;Do, Jeong Tae;Chae, Jung-il;Choi, Hyun Woo
    • Journal of Microbiology and Biotechnology
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    • v.32 no.10
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    • pp.1245-1252
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    • 2022
  • Induced pluripotent stem cells (iPSCs) can be generated from somatic cells using Oct4, Sox2, Klf4, and c-Myc (OSKM). Small molecules can enhance reprogramming. Licochalcone D (LCD), a flavonoid compound present mainly in the roots of Glycyrrhiza inflata, acts on known signaling pathways involved in transcriptional activity and signal transduction, including the PGC1-α and MAPK families. In this study, we demonstrated that LCD improved reprogramming efficiency. LCD-treated iPSCs (LCD-iPSCs) expressed pluripotency-related genes Oct4, Sox2, Nanog, and Prdm14. Moreover, LCD-iPSCs differentiated into all three germ layers in vitro and formed chimeras. The mesenchymal-to-epithelial transition (MET) is critical for somatic cell reprogramming. We found that the expression levels of mesenchymal genes (Snail2 and Twist) decreased and those of epithelial genes (DSP, Cldn3, Crb3, and Ocln) dramatically increased in OR-MEF (OG2+/+/ROSA26+/+) cells treated with LCD for 3 days, indicating that MET effectively occurred in LCD-treated OR-MEF cells. Thus, LCD enhanced the generation of iPSCs from somatic cells by promoting MET at the early stages of reprogramming.