• 생약학회지
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• 제40권4호
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• pp.376-381
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• 2009

The compositional analysis and the peroxynitrite ($ONOO^-$) scavenging assay were undertaken to demonstrate beneficial dietary effect of lettuce (Lactuca sativa L., Compositae). Quinic acid derivatives of the seven cultivars were qualitatively and quantitatively analyzed by HPLC to lead to the finding of 3,4-di-O-caffeoyquinic acid and 3-O-p-coumaroylquinic acid and their contents ranged over 2.72-4.47 mg/g and 8.97-23.26 mg/g, respectively. A cultivar Hacheong had the highest quantity of quinic acid derivatives. The peroxynitrite scavenging effect of the five cultivars (Jangsu, Gosina 27, Gopung, Yeolpungjeogchima, and Mipungjeogchugmyeon) were ranged over 7.45-8.26${\mu}g$/ml as $IC_{50}s$ while those of Hacheong and Mihong had less effect ($IC_{50}$ >10 ${\mu}g$/ml).

• BMB Reports
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• 제40권6호
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• pp.870-874
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• 2007

We cloned a uridine-diphosphate dependent glycosyl-transferase RUGT-10 from Oryza sativa. The recombinant enzyme was expressed by glutathione-S transferase gene fusion system in Escherichia coli. RUGT10 showed different regioselectivity depending on the structures of substrates (e.g. flavanone, flavonol, and flavone). Apparently, flavanone such as naringenin and eriodictyol gave one 7-O-glucoside while flavone and flavonol gave more than two products with preferential glucosylation position of hydroxyl group at C-3 position.

• Plant Resources
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• 제7권1호
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• pp.69-76
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• 2004

Lettuce (Lactuca sativa L.) is known to contain water-soluble substances that are biologically active. Aqueous or methanol extracts and residues from leaves of lettuce plants were assayed to determine their allelopathic effects, and the causative allelochemicals from fractions were quantified by means of HPLC analysis and bioassayed. Extracts from oven-dried leaf samples were more phytotoxic than those from freeze-dried samples. Leaf extracts of 40 g L$^{-1}$ were completely inhibitory on root growth of alfalfa (Medicago sativa L.), while root growths of barley (Hordeum vulgare L.) and soybean (Glycine max L.) were less sensitive. Early seedling growth of both alfalfa and barnyard grass (Echinochloa crus-galli) was significantly reduced by methanol leaf extracts. The major allelopathic substances analyzed by HPLC were coumarin, trans-cinnamic acid, o-coumaric acid, p-coumaric acid and chlorogenic acid. Of them p-coumaric acid was found as the greatest amount (8.9 mg 100 g$^{-1}$ ) in the EtOAc fraction; only coumarin was found in all the fractions. Hexane and EtOAc fractions of L. sativa reduced alfalfa root growth more than did BuOH and water fractions. These results suggest that lettuce had potent herbicidal activity, and that its activity differed depending on type and amount of causative compounds by fraction.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 춘계학술발표회 요지
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• pp.34-35
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• 2005

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 춘계학술발표회 요지
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• pp.214-215
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• 2005

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 추계학술발표회 요지
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• pp.130-131
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• 2005

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.999-999
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• 2005

• 한국광과학회:학술대회논문집
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• 한국광과학회 2006년도 정기총회 및 제13회 학술대회 논문 초록
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• pp.23-23
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• 2006

• The Plant Pathology Journal
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• 제37권4호
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• pp.375-388
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• 2021

Rice yellow mottle virus (RYMV) is the most harmful virus that affects irrigated and lowland rice in Africa. The RBe24 isolate of the virus is the most pathogenic strain in Benin. A total of 79 genotypes including susceptible IR64 (Oryza sativa) and the resistant TOG5681 (O. glaberrima) as checks were screened for their reactions to RBe24 isolate of RYMV and the effects of silicon on the response of host plants to the virus investigated. The experiment was a three-factor factorial consisting of genotypes, inoculation level (inoculated vs. non-inoculated), and silicon dose (0, 5, and 10 g/plant) applied as CaSiO₃ with two replications and carried out twice in the screen house. Significant differences were observed among the rice genotypes. Fifteen highly resistant and eight resistant genotypes were identified, and these were mainly O. glaberrima. Silicon application did not affect disease incidence and severity at 21 and 42 days after inoculation (DAI); it, however, significantly increased plant height of inoculated (3.6% for 5 g CaSiO₃/plant and 6.3% for 10 g CaSiO₃/plant) and non-inoculated (1.9% for 5 g CaSiO₃/plant and 4.9% for 10 g CaSiO₃/plant) plants at 42 DAI, with a reduction in the number of tillers (12.3% for both 5 and 10 g CaSiO₃/plant) and leaves (26.8% for 5 g CaSiO₃/plant and 28% for 10 g CaSiO₃/plant) under both inoculation treatments. Our results confirm O. glaberrima germplasm as an important source of resistance to RYMV, and critical in developing a comprehensive strategy for the control of RYMV in West Africa.

• 생명과학회지
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• 제28권8호
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• pp.908-916
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• 2018

모발의 검정색은 노화됨에 따라 백색으로 변화된다. 이러한 현상은 모낭내 tyrosinase 활성의 감소와 $H_2O_2$에 의한 축적에 의하여 기인된다. 따라서 본 연구의 목적은 현미주정추출물(OREE)를 이용하여 백발화의 원인인 과산화 수소에 대한 항산화 활성과 멜라닌 생성 촉진 효과를 조사한 것이다. 본 연구에서 OREE은 낮은 DPPH radical 소거능과 환원력을 보여주었다. 그러나 B16F1 세포내에서 $H_2O_2$ 소거에 대해서는 높은 항산화효과를 나타내었다. 그리고 OREE는 in vitro에서 DOPA 산화 활성은 나타나지 않았지만 $64{\mu}g/ml$에서 tyrosinase 활성을 증가시켰다. MTT assay에서 OREE는 $32{\mu}g/ml$ 이상의 농도에서 세포독성을 나타내었다. 또한 OREE는 $8{\mu}g/ml$ 이상의 농도에서 멜라닌 합성은 농도에 비례하여 증가하였고, $H_2O_2$로 멜라닌 생성을 저하시킨 세포에서도 멜라닌 합성을 증가시켰다. 멜라닌 합성에 대한 OREE의 효과를 확인하기 위하여 Western blot 분석이 수행되었다. $H_2O_2$로 멜라닌 생성을 저하시킨 세포에서 멜라닌 합성 기전 관여하는 tyrosine hydroxylase와 tyrosinase-related protein-2 (TRP-2) 발현은 OREE의 존재 하에서 증가하였다. 이상의 발견들은 OREE가 멜라닌 합성을 촉진시킬 수 있어 이와 관련된 모발화장품의 개발에 적용시킬 수 있다는 것을 시사하고 있다.