• Asian-Australasian Journal of Animal Sciences
• /
• v.33 no.6
• /
• pp.930-940
• /
• 2020

Objective: This study was conducted to evaluate changes in nutritional value and in situ dry matter (DM) degradability of oak and pine wood before and after steam-digestion process (60 min/160℃/6 atm) and feeding effect of the oak roughage on performance and behavior of Hanwoo steers. Methods: Chemical composition and tannin concentration were analyzed for oak and pine trees before and after the pretreatment. In situ DM and effective degradability of these samples were assessed using a nylon bag method. In vivo trial was performed to estimate animal performance and behavior, using steers fed total mixed ration (TMR) diets containing 0% (control), 25% (OR-25), and 50% (OR-50) of the oak roughage. Eighteen steers were allocated into nine pens (2 steers/pen, 3 pens/treatment) for 52 days according to body weight (BW) and age. Results: By the steam-digestion treatment, the neutral detergent-insoluble fiber was decreased from 86.5% to 71.5% for oak and from 92.4% to 80.5% for pine, thereby increasing non-fiber carbohydrate. In situ DM degradability of treated oak reached 38% at 72 h, whereas that of untreated oak was only 11.9%. The 0 h degradability of the treated pine increased from 5.9% to 12.1%, but the degradability was unchanged thereafter. Animal performance including BW, average daily gain, DM intake, and feed conversion ratio was not different among control and oak treatments. No differences were detected in animal behavior such as lying, standing, rumination, drinking, and eating, except walking. Walking was higher in control than oak treatments with numerically higher eating and lower lying times, probably due to bulkier characteristics of rice straw in the diet. Conclusion: This study demonstrates that the oak roughage can be substituted for 50% of total forage or 100% of rice straw in TMR diets at early fattening stage of Hanwoo steers.

• Food Science of Animal Resources
• /
• v.35 no.6
• /
• pp.765-771
• /
• 2015

The development of a sample preparation method and optimization of the analytical instrumentation conditions were performed for the determination of the vitamin B₁₂ content in emulsified baby foods sold on the Korea market. After removal of the milk protein and fats by chloroform extraction and centrifugation, the vitamin B₁₂ was water extracted from the sample. Following filtration of the solution through a nylon filter, the water-soluble extract was purified by solid-phase extraction using a Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). The solution eluted from the cartridge was dried under a stream of nitrogen gas and reconstituted with 1 mL of water. The sample solution was injected into an LC-MS/MS system after optimizing the mobile phase for vitamin B₁₂ detection. The calibration curve showed good linearity with the coefficient of correlation (r²) value of 0.9999. The limit of detection was 0.03 µg/L and the limit of quantitation was 0.1 µg/L. The method of detection limit was 0.02 µg/kg. The vitamin B₁₂ recovery from a spiking test was 99.62% for infant formula and 99.46% for cereal-based baby food. The sample preparation method developed in this study would be appropriate for the rapid determination of the vitamin B₁₂ content in infant formula and baby foods with emulsified milk characteristics. The ability to obtain stable results more quickly and efficiently would also allow governments to exercise a more extensive quality control inspection and monitoring of products expected to contain vitamin B₁₂. This method could be implemented in laboratories that require time and labor saving.

• The Korean Journal of Quaternary Research
• /
• v.17 no.2
• /
• pp.163-163
• /
• 2003

The study area, Kwangneung Experiment Forest (KEF) is located on the west-central portion of Korean peninsula and belongs to a cool-temperate broadleaved forest zone. At the old-growth deciduous forest near Soribong-peak (533.1m) in KEF, we have established a permanent plot and a flux tower, and the site was registered as a KLTER site and also a KoFlux site. In this study, we aimed to present basic ecological characteristics and synthetic data of carbon budgets and flows, and some monitoring data which are essential for providing important parameters and validation data for the forest dynamics models or biogeochemical dynamics models to predict or interpolate spatially the changes in forest ecosystem structure and function. We made a stemmap of trees in 1 ha plot and analyzed forest stand structure and physical and chemical soil characteristics, and estimated carbon budgets by forest components (tree biomass, soils, litter and so on). Dominant tree species were Quercus serrata and Carpinus laxiflora, and accompanied by Q. aliena, Carpinus cordata, and so on. As a result of a field survey of the plot, density of the trees larger than 2cm in DBH was 1,473 trees per ha, total biomass 261.2 tons/ha, and basal area 28.0 m2/ha. Parent rock type is granite gneiss. Soil type is brown forest soil (alfisols in USDA system), and the depth is from 38 to 66cm. Soil texture is loam or sandy loam, and its pH was from 4.2 to 5.0 in the surface layer, and from 4.8 to 5.2 in the subsurface layer. Seasonal changes in LAI were measured by hemispherical photography at the l.2m height, and the maximum was 3.65. And the spatial distributions of volumetric soil moisture contents and LAIs of the plot were measured. Litterfall was collected in circular littertraps (collecting area： 0.25m2) and mass loss rates and nutrient release patterns in decomposing litter were estimated using the litterbag technique employing 30cm30cm nylon bags with l.5mm mesh size. Total annual litterfall was 5,627 kg/ha/year and leaf litter accounted for 61% of the litterfall. The leaf litter quantity was highest in Quercus serrata, followed by Carpinus laxiflora and C. cordata, etc. Mass loss from decomposing leaf litter was more rapid in C. laxiflora and C. cordata than in Q. serrata litter. About 77% of C. laxiflora and 84% of C. cordata litter disappeared, while about 48% in Q. serrata litter lost over two years. The carbon pool in living tree biomass including below ground biomass was 136 tons C/ha, and 5.6 tons C/ha is stored in the litter layer, and about 92.0 tons C/ha in the soil to the 30cm in depth. Totally more than about 233.6 tons C/ha was stored in DK site. And then we have drawn a schematic diagram of carbon budgets and flows in each compartment of the KEF site.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.32 no.3
• /
• pp.338-344
• /
• 1999

Floating debris was recorded from a training ship, $\sharp$1 Kwanaksan, of Pukyong National University with about 10 knots speed at July 15th and 20th of 1997. The sampled area is the middle of southern sea of Korea, divided into 44 unit segments on survey routes. Debris fabrication materials were categorized with 6 items using the following; man-made or natural wood items, paper and cardboard, nylon netting and rope, styrofoam, plastics, floating metal and glass containers. All identified items within a 100 $\pm$ 2 m wide band were recorded but ignored if beyond this boundary. The results of distribution and transport of floating debris in the area are as follows: 1. The quantities of debris during the survey were distributed from $1.6\~369.7\;items/km^2$. The most obvious trend is the widespread distribution of all debris. The highest densities of all debris were discovered in the coastal waters of Namhae and Yokji island, and of about 50 km off from the southward of Yokji and about 74 km off from the eastward of Komun island. Especially many of small styrofoams within $\phi$20 cm were observed in these segments. 2. Styrofoams and plastics were composed of $83.5\%$ among all debris, next woods items, $9.8\%$. 3. The quantities, distribution shapes and composition of debris were varied as the observed duration and the natures of each items. 4. These phenomena are concluded that firstly they depend on the river discharges included debris due to precipitation falls, secondly inflow or dumping debris are drifting to the off-shore by Kuroshio currents present at their adjacent sea, But on the basis of the observed data it is difficult that source position, quantities and inflow items of debris are identified, and also the transport processes is pursue. further more surveys are continuously being investigated, and from this it is hoped that a much wider coverage can be achieved, perhaps on all sites of the Coast of Korea and contributed to the stationary area, finding of sources, removal method of debris and resistants of marine productivity.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.18 no.3
• /
• pp.527-533
• /
• 2017

As the clothing industry has advanced, dyeing technologies using various dyes have been developed. In recent years, interest in natural pigments has been increasing because of the negative impact of synthetic pigment on human health; therefore, development and application of microbial pigments is demanded. In this study, the dyeing effects on multifiber fabrics and biological activity were assessed using violet natural pigment from the marine bacterium, Microbulbifer sp. PPB12. The violet pigment produced by cultivation of Microbulbifer sp. PPB12 using Marine broth 2216 for 3 days was extracted using ethanol. Once dissolved in 20% ethanol, the violet pigment could be used to dye bleached cotton, diacetate, and especially polyamide. The optimal temperature, time, pH, and bath ratio under the dyeing conditions were $80^{\circ}C-90^{\circ}C$, more than 1 hour, pH 4-6, and 1:25, respectively. The mordant treatment was more suitable for color expression when $Na_2SO_4$ was used after 10 minutes of dyeing, but no significant difference was observed from untreated samples. The violet pigment also showed antibacterial activity against B. subtilis. The results of the present study indicate that the marine bacterial pigment could be an alternative for textile dyeing as a natural dye with antibacterial activity.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.38 no.12
• /
• pp.1767-1772
• /
• 2009

Brined Baechu cabbages were packed with different films of high density polyethylene (HDPE), aluminium polyethylene (Al/PE), nylon polyamide (Ny/PE), low density polyethylene (LDPE) and permeability-controlled polyethylene (Mirafresh (MF), US patent No. 5972815), and then stored at 4${^{\circ}C}$ for 4 weeks. Changes in quality characteristics of pH, acidity, total bacteria counts, lactic acid bacteria counts, E. coli counts, texture and $O_2$ concentration were determined during the storage. The pH of brined Baechu cabbage packed with Mirafresh (MF) film was 6.25 after 4 weeks from initial pH of 6.80. The acidity of all brined Baechu cabbages increased, however, the increase of the cabbage in MF was the lowest. The levels of total bacteria, lactic acid bacteria and E. coli in the cabbages packed with MF were also lower than the other films. After 4 weeks, of all brined springiness Baechu cabbages decreased, but MF showed relatively high springiness. The $O_2$ concentrations by its permeation through MF were 0.35%-1.00% at 4-25${^{\circ}C}$ after 1 week. In conclusion, MF was found to be the most effective packaging film for brined Baechu cabbage to extend shelf-life.

• Composites Research
• /
• v.29 no.6
• /
• pp.408-413
• /
• 2016

Commonly-used polymers are manufactured as versatile forms. Furthermore, continuous polymer fibers are recently manufactured using nylon or aramid fiber. One of common epoxy was also used to make polymer fibers. Bisphenol-A type was used as base epoxy whereas amine and anhydride were used as hardeners. Epoxy fibers was cured by stepping up the temperature to maintain the shape of epoxy fiber. Surface energy was measured to confirm the degree of interfacial adhesion by modified static contact angle method. After mechanical properties were measured via fiber tensile test, the evaluation of fiber fracture was proceeded. Tensile strength of epoxy fiber using amine type hardener was higher as 138 MPa than anhydride case as 70 MPa. Fractured surface exhibited different failure patterns at the cross-section.

• Asian-Australasian Journal of Animal Sciences
• /
• v.11 no.6
• /
• pp.673-679
• /
• 1998

The experiment was conducted to determine in situ rumen degradability of soybean meal (SM), fish meal (FM), sesame cake (SC) and Italian ryegrass hay (IRGH) and the effect of supplementing the above protein meals to IRGH on digestibility and balance of nutrients in three Saanen goats. For measuring the degradability, nylon bags containing each meal were incubated in the rumen of one fistulated dry cow for 3, 6, 12, 24, 36, and 48 hrs. Degradability revealed that SM protein was highly degradable in the rumen (99.1%), while FM protein was less degradable (76.8%) with SC protein being intermediate (91.2%) at 48 hrs of incubation (p < 0.01). Degradation rate of the potentially degradable fraction was estimated to be 12.12, 5.88 and 5.88%/hr for SM, FM and SC, respectively. In the metabolism trial, all goats were offered daily 900 g IRGH and one of the supplements, SM (100 g), FM (75 g) or SC (100 g). Intake of DM, OM, CP and GE was similar among diets. However, digestibility of OM for SC diet was significantly lower than that for diets supplemented with FM and SM (p < 0.10). Nitrogen (N) excreted in faeces (p < 0.05) and in urine (p < 0.10) was, respectively, higher and lower for SC diet than that for the other two diets. The same tendency was observed in energy losses in faeces (p < 0.10) and in urine (p < 0.05). There was no difference in energy loss in methane or in heat production among diets. Consequently, no significant difference was observed in N retention (2.13, 0.42 and -0.11 g/day for FM, SC and SM diet, respectively) or in energy retention (-1.49, -2.14 and -2.70 MJ/day for FM, SM and SC diet, respectively). These results showed that protein supplements affected the digestion of diets based on grass hay with 7.45% CP in DM in goats, although there was no significant influence on N or energy retention.

• The Korean Journal of Food And Nutrition
• /
• v.15 no.3
• /
• pp.197-202
• /
• 2002

This study was carried out to investigate food additives, physico-chemical, sanitary state of the marketing jerky in domestic. The kind of sensory agents and food additives on the marketing jerky sample used 16~23 kinds, and the domestic processed by import beef was large number, but that domestic pork jerky was small number The moisture content on the import beef jerky were high, and crude protein on the import beef jerky and domestic pork jerky were high level, and crude fat on the domestic pork jerky were high, but crude ash on all jerky sample were similar level. Degree of saline on the import beef jerky was high, but the domestic beef jerky was low. The range of pH was 5.41~6.11, and that import beef jerky was little high, but the domestic beef jerky was low. Water activity value in pork jerky was 0.743, also had high, and domestic beef jerky was lowest than others jerky sample. Range of VBN value in jerky sample was 9.98~12.36mg%, and the range TBA value was 0.239~0.367, and that value showed difference in all jerky sample. The total general bacteria count of marketing jerky was 10$^3$~ 10$^4$ CFU/g, and import beef jerky was less higher than domestic beef and pork jerky.

• The Korean Journal of Food And Nutrition
• /
• v.15 no.3
• /
• pp.167-202
• /
• 2002

This study was carried out to investigate food additives, physico-chemical, sanitary state of the marketing jerky in domestic. The kind of sensory agents and food additives on the marketing jerky sample used 16~23 kinds, and the domestic processed by import beef was large number, but that domestic pork jerky was small number The moisture content on the import beef jerky were high, and crude protein on the import beef jerky and domestic pork jerky were high level, and crude fat on the domestic pork jerky were high, but crude ash on all jerky sample were similar level. Degree of saline on the import beef jerky was high, but the domestic beef jerky was low. The range of pH was 5.41~6.11, and that import beef jerky was little high, but the domestic beef jerky was low. Water activity value in pork jerky was 0.743, also had high, and domestic beef jerky was lowest than others jerky sample. Range of VBN value in jerky sample was 9.98~12.36mg%, and the range TBA value was 0.239~0.367, and that value showed difference in all jerky sample. The total general bacteria count of marketing jerky was 10$^3$~ 10$^4$ CFU/g, and import beef jerky was less higher than domestic beef and pork jerky.