• Title/Summary/Keyword: Nutrient substrate

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Production of red pigments by Monascus purpureus in solid-state culture

  • Park, Hae-Yeon;Lee, Beom-Gyu;Jeong, Uk-Jin
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.315-316
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    • 2001
  • In this study various nutritional and environmental parameters such as, initial moisture content, pH. inoculum size, air rate, sample size and nutrient supplement that influence pigment production were evaluated in solid-state cultures. optimum initial moisture content and pH were determined to be 50% and 6.0, respectively. The supplement of the substrate with different carbon, nitrogen, and mineral source reveals a more inhibitive effect as the substrate concentration increase. optimum aeration rate was determined to be 2vvm in flask culture. The maximum amount of red pigment, 3500 OD/g dried fermented rice, was obtained in optimum conditions which is obtained in solid flask culture.

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The Effect of Pulse Electric Field on Accumulation of Selenium in Cells of Saccharomyces cerevisiae

  • Pankiewicz, Urszula;Jamroz, Jerzy
    • Journal of Microbiology and Biotechnology
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    • v.17 no.7
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    • pp.1139-1146
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    • 2007
  • Cultures of Saccharomyces cerevisiae were subjected to the effect of PEF (pulse electric field) and a source of selenium. The culture period after which yeast cells were subjected to PEF treatment was optimized, as was the duration of the exposure. Optimization of the nutrient medium composition in S. cerevisiae cultures resulted in an over 1.8-fold increase in selenium accumulation with relation to cultures on the initial substrate. Optimization of the pH value and of culture duration resulted in selenium accumulation increase by approximately 78%. A significant correlation was found between the accumulation of selenium in yeast cells and its concentration in the culture substrate. The highest accumulation of selenium in the biomass of yeast, approx. $240\;{\mu}g/g$ d.m., was obtained after 15-min exposure to PEF on a 20-h culture. An approx. 50% higher content of selenium in cells was recorded, as compared with the control culture without the application of PEF.

Production of Red Pigments by Monascus purpureus in Solid-state Culture

  • Lee Bum-Kyu;Piao Hai Yan;Chung Wook-Jin
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.1
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    • pp.21-25
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    • 2002
  • To maximize and sustain the productivity of Monascus pigments, various environmental and nutritional parameters, such as the initial moisture content, pH, inoculum size, sample size, and nutrient supplement, that influence pigment production were evaluated in solid-state cultures as follows: initial moisture content, $50\%;$ pH, 6.0; inoculum size $1\;\times\;10^4$ spore cells $(grams\;of\;dry\;solid\;substrate)^{-1};$ sample size, 300 g. All supplementary nutrients (carbon, nitrogen, and mineral sources) added has inhibitory effects on the cell growth and red pigment production. In open tray culture the maximum biomass yield and specific productivity of red pigments were 223 mg DCW $(grams\;of\;initial\;dry\;substrate)^{-1}$ and, $47.6\;OD_{500}\;(DCW\;grams)^{-1}h^h{-1}$ respectively.

Development of Optimal Nutrient Solution of Tomato(Lycopercicon esculentum Mill.) in a Closed Soilless Culture System (순환식 수경재배에 적합한 토마토 배양액 개발)

  • Yu, Sung-Oh;Bae, Jong-Hyang
    • Journal of Bio-Environment Control
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    • v.14 no.3
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    • pp.203-211
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    • 2005
  • The experiment was conducted to investigate the nutrition absorption pattern in the growth stages and develope the optimal nutrient solution hydroponically grown the tomato in closed substrate culture system with the nutrient solution of National Horticultural Research Station in Japan into 1/2S, 1 S, and 2S. When plant was grown in 1/2 S, the growth and yield were high and the pH and EC in the rooting zone were stable. Suitable composition of nutrient solution for tomato was $NO_3-N$ 7.1, $PO_{4}$-P 2.1, K 4.0, Ca 3.1, Mg 1.2, and $SO_{4}-S\;1.2\;me{\cdot}L^{-1}$ in the early growth stage and $NO_3-N$ 6.5, $PO_4-P$ 2.3, K 3.4, Ca 3.1, Mg 1.1, and $SO_4-S\;1.1\;me{\cdot}L^{-1}$ in the late growth stage by calculating a rate of nutrient and water uptake. To estimate the suitability for the nutrient solution of tomato in a development of optimum nutrient solution of tomato developed by Wonkwang university in korea (WU), plant was grown in perlite substrate supplied with different solution and strengths(S) by research station for greenhouse vegetable and floricultuin in the Netherlands (Proefstation voor tuinbouw onder glas te Naaldwijk; PTG) of 1/2 S, 1 S and 2 S, respectively, The growth was good at the PTG and WU of 2 S in early growth stage, and at the WU 2S in late growth stage. The highest yield of tomato obtained in the WU of 2 S, although blossom-end rot was appeared in all treatments. pH and EC in root zone of WU of 2 S were stable during the early and late growth stage. Therefore when plant was grown in WU of 2 S, N and P content in the nutrient solution need to low, according N and P content of their leaves were high in WU of 2 S.

Growth and Postharvest Quality of Hydroponic Pot Rose 'Silk Red' as Affected by ionic Strength of Nutrient Solution and Content of Natural Soil in Growing Substrate (영양액의 이온농도와 배지내 천연토양의 함유량이 양액재배 분화장미의 생육과 수확 후 품질에 미치는 영향)

  • 조지영;하홍석;정병룡
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 2001.11a
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    • pp.102-115
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    • 2001
  • 국내 분화류의 생산면적은 2000년 현재 1,036.2ha이며 생산량은 연간 282,656천분, 그리고 생산액은 연간 268,498,990천원으로 추산되고 있다(2000년 화훼재배현황, 농림부). 이와 같은 국내 분화재배면적이 증가함에 따라 다양한 화종의 분화가 재배되고 있으며 화종에 따른 관수방법, 이용상토, 양액농도, 시비방법 등에 대한 많은 연구가 이루어지고 있다(Lieth and Burger, 1989). (중략)

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Development of Nutrient Solution for Cucumber Substrate Culture in a Closed Growing System (오이 순환식 고형배지경에 적합한 배양액개발)

  • 이용범;노미영;김회상;이경복;최은영
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 1996.10a
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    • pp.67-70
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    • 1996
  • 오이의 양액재배는 초기에 암면을 중심으로 한 배지경에서 최근 펄라이트를 주 배지로 한 배지경의 면적이 급속히 증가하고 있다. 이들 고형배지를 이용한 양액재배에 사용한 배양액은 크게 2종류로 구분할 수 있다. 하나는 일렬의 야먀자끼씨의 오이 배양액으로 이 배양액은 담액수경하에서 개발된 배양액으로 순수 수경재배에 적합하다고 할 수 있다. 다른 하나는 네델란드 온실작물연구소(PBG)의 오이배지경용 배양액이라 할수 있다. (중략)

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Effects of Habitat and Nutrient Content of Leaves on the Litter Decomposition of Larix kaempferi and Quercus serrata at Kwangnung (광릉의 잎갈나무(Larix kaempferi)와 졸참나무(Quercus serrata) 낙엽의 분해에 미치는 잎의 영양함량과 입지의 영향)

  • 박봉규
    • Journal of Plant Biology
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    • v.23 no.2
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    • pp.45-48
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    • 1980
  • Effects of habitat and substrate quality on decomposition rate of litters of Larix kaempferi and Quercus serrata were estimated in Kwangnung forest. The amount of organic matter under the canopy of Quercus serrata stand was higher than that under the canopy of Larix kaempferi. The loss constant of litters in the Larix kaempferi stand was higher than that in the Quercus serrata stand.

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Effects of Mixing Ratio of Substrates and Concentration of Fertigation in Nutrient Solutions on the Early Growth of Cucumber Plug Seedlings (배지의 혼입비율과 관비 양액 농도가 오이 플러그묘의 초기생장에 미치는 영향)

  • Kim, Hong-Gi;Cho, Ja-Yong;Bae, Jong-Hyang;Yang, Seung-Yul;Heo, Buk-Gu
    • Journal of Bio-Environment Control
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    • v.16 no.2
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    • pp.101-107
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    • 2007
  • This experiment was carried out to develop the substrate mixtures for the production of high quality plug seedlings of cucumber. Peatmoss based substrates, rice hull, carbonized rice hull, decomposed sawdust, perlite and granular rockwool were mixed by five different mixing ratioes (M1, M2, M3, M4 and M5). The cultivars used were cucumber (Cucumis sativus L. cv. Janghyung heukjinju) plants. The higher the content of peatmoss added, the higher the plant growth in terms of plant height, leaf area and total dry weight, which leading to the production of high quality plug seedlings. Seedlings growth of cucumber were greater in M5 mixtures [peatmoss:rice hull:decomposed sawdust=40:40:20(v/v)], M4 mixtures [peatmoss:rice hull:decomposed sawdust:granular rockwool=30:25:20:25(v/v)] and M2 mixtures [peatmoss:rice hull:decomposed sawdust:granular rockwool=20:20:15:25:20(v/v)] The concentrations of nutrient solution (EC) had a great influence on plant height, leaf area, total fresh and dry weight of cucumber seedlings growth. As the concentration of nutrient solution increased from 0.1 to $1.5dS{\cdot}m^{-1}$, the growth and seedling quality of cucumber in plant height, leaf area and dry weight were significantly improved.

Changes of Nutrient Content of Circulating Solution in Three Different New Hydroponics for Oriental Melons(Cucumis melo L. var. makuwa Mak.) (참외용 수경재배시스템에서의 순환배양액의 무기이온 함량의 변화)

  • 조익환;전하준
    • Journal of Bio-Environment Control
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    • v.11 no.4
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    • pp.168-174
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    • 2002
  • A new circulating hydroponic system was invented for oriental melons grown in the greenhouse. For developing nutrient solution management techniques, we examined the changes of nutrient contents of circulating solution in three different types of new hydroponic systems. The yield and fruit quality of oriental melons in Hydroponics were better than those in soil culture. The substrate culture was appropriate fer hydroponics of oriental melons, and NFT was turned to be the opposite due to the physiological disorder during hot seasons. Yamazaki's melon solution with EC 2.0dS.m$^{[-10]}$ was the most appropriate for oriental melons. The new circulating hydroponic system seemed to be appropriate for oriental melons because of the stable EC, pH and the macro- and micro-element contents. NO$_3$-N, Ca and Mg contents in the circulating solution kept a good balance in all types of hydroponics. However, p content, compared to other types, decreased by the degree of 1 me.L$^{[-10]}$ in perlite medium. K content showed irregular status in perlite but showed the stable status in cocopeat. Generally, microelements, except Mo, showed stable absorption in the substrate culture. However, in NET, most of the elements showed irregular absorption except B and Mn. Microelement absorption, especially Cu, Zn and Mo, decreased during hot seasons.

Quantification of Rockwool Substrate Water Content using a Capacitive Water Sensor (정전용량 수분센서의 배지 함수량 정량화)

  • Baek, Jeong-Hyeon;Park, Ju-Sung;Lee, Ho-Jin;An, Jin-Hee;Choi, Eun-Young
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.27-36
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    • 2021
  • A capacitive water sensor was developed to measure the capacitance over a wide part of a substrate using an insulated electrode plate (30 cm × 10 cm) with copper and Teflon attached on either side of the substrate. This study aimed to convert the capacitance output obtained from the condenser-type capacitance sensor into the substrate water content. The quantification experiment was performed by measuring the changes in substrate water weight and capacitance while providing a nutrient solution and by subsequently comparing these values. The substrate water weight and capacitance were measured every 20 to 30 seconds using the sensor and load cell with a software developed specifically for this study. Using a curve-fitting program, the substrate water content was estimated from the output of the capacitance using the water weight and capacitance of the substrate as variables. When the amount of water supplied was increased, the capacitance tended to increase. Coefficient of variation (CV) in capacitance according to the water weight in substrate was greater with the 1.0 kg of water weight, compared with other weights. Thus, the fitting was performed with higher than 1.0 kg, from 1.7 to 6.0 kg of water weight. The correlation coefficient between the capacitance and water weight in substrate was 0.9696. The calibration equation estimated water content from the capacitance, and it was compared with the substrate water weight measured by the load cell.