• Journal of Bio-Environment Control
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• v.8 no.3
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• pp.172-182
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• 1999

Leaf lettuce(Lacaug sativa L.) was cultivated in deep flow culture to investigate growth and yield in relation to different reusing method of nutrient solution after once cultivation. Five different treatments were allocated to the nutrient solutions - Control(total renewal of solution), NSS(nutrient solution supplement), U control, NSAC(nutrient solution analysis and compensation), NSAC and Humus supply(NSAC with supply of Humus). The pH of solution was kept stable below 7.0 during 4 successive culture in NSAC and NSAC and Humus supply. U was sharply declined in NSS as the number of cultivation was increased. Gmwth and yield of NSAC was similar to those of Control because nutrient elements were kept the balance to the better growth, while the lettuce grown in NSS and EC control was shown lower growth rate. In the nutrient solutions, Content of N $O_3$-N and N $H_4$-N were remarkably decreased after the cultivation in all treatments. Ca and Mg were shown to be accumulated in nutrient solution regardless of culture times and treatments. After the first culture in NSAC and Humus supply, total N and P$_2$ $O_{5}$ content in leaves were lower than any other treatments, but Ca content was higher. Those were not significant as following cultures, and no significant difference of K and Mg content were shown among the treatments.

• Journal of Bio-Environment Control
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• v.17 no.4
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• pp.252-260
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• 2008

This study was conducted to observe the characteristics of mineral nutrient uptake of single-node cutting rose 'Versilla' and to determine optimal nutrient solution control method for soilless culture of 'Versilla' in a closed hydroponic system. Nutrient solution was managed by five different control methods: macro- and micro-element control in aeroponic system (M&M), macroelement control in aeroponic system (M), nutrient solution supplement in aeroponic system (S); electrical conductivity (EC) control in aeroponic system(EC-A); EC control in deep flow technique system(EC-D). The concentration of $NO_3$-N exceeds optimal range whereas P and Mg decreased at the later stage of plant growth with the EC control method, EC-A and EC-D. The overall mineral nutrient content increased with S. On the other hand the nutrient content at the root environment was maintained optimal with M&M and M. The nutrient solution control methods had significantly effect on the cut-flower quality. In the M&M and M, flower length, fresh weight and root activity were higher than those with the other mineral nutrients control methods. The maximal efficiency of photochemistry (Fv/Fm) was higher for M&M, M and S than that with EC-A and EC-D. Based on the above results, it is highly recommended to control nutrient solution by mineral nutrient control methods (M&M and M) in a closed hydroponic system for single-node cutting rose, 'Versillia'.

• Journal of Bio-Environment Control
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• v.5 no.1
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• pp.43-49
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• 1996

This study was conducted to investigate the effects of artificial zeolite and various ion exchangers supplemented in rockwool on the ion exchangability of muskmelon. The results obtained were as follows ; Plant height was higher in the treatment of Ca type artificial zeolite as of 131.2cm than that of control as of 124cm, and same trends were shown in fresh weight of leaf and stem. Fruit weight was increased by supplement of artificial zeolite, but there were no significant differences in the sugar degree and external appearance as influenced by supplementing artificial zeolite. Supplement of artificial zeolite stabilized the pH and increased the ion exchange capacity of nutrient solution. Nutrient absorption was more favorable and led to growth promotion. This study was demonstrated that supplement of artificial zeolite in rockwool slab was improved the stabilization of root environment and increased ion uptake of muskmelon plant.

• Korean Journal of Community Nutrition
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• v.2 no.4
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• pp.624-632
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• 1997

This study was conducted to evaluated the nutrition quality of the commercial supplementary foods for infants and young children and to seek a solution to the establishment of standards of nutrient requirements for supplementary foods in Korea. Information on food ingredients, nutrient contents, claims about usefulness of food components and instructions for feeding preparation were obtained from the labels of 33 commercial supplementary foods manufactured by 4 different domestic companies. According to the standard of supplementary foods for infants and young children described in the Korean Food Code, the commercial supplementary foods were categorized into two different types, weaning food and baby food. All the commercial weaning foods were in powder form and mainly composed of cereals, whereas all the baby foods were mainly composed of fruits in the form of canned juice. The weaning foods contained more nutrients than the baby foods did, and the nutrient levels of the weaning foods expressed as nutrient density on energy basis were higher than the RDA for infants aged 5 to 11 months, suggesting that the commercial weaning foods provide adequate amounts of nutrients. If one followed the instructions for feeding preparation appearing on the label, however, recommended amounts of intake of the weaning foods would provide too much energy as well as nutrients. There were many differences in nutrient standards of weaning foods between the Korean Food Code and Codex international food standard. In conclusion, the establishment of standards for nutrient requirements for the supplementary foods requires significant scientific studies on what nutrients are the most inadequate in Korean infants and young children feeds and what levels of nutrients should be added to the foods in order to supplement their nutrition. In addition, it is very important to have a strong scientific basis to support our standard when discrepancies exist between our standard and the international standard. (Korean J Community Nutrition 2(4) : 624-632, 1997)

• Journal of Bio-Environment Control
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• v.12 no.2
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• pp.89-94
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• 2003

This study was conducted to identify the effect of recycling method of drained solution on the concentration of drained solution, and growth and yield of tomato in the recycling system. The recycling methods of drained solution were composed of control, MEC (measurement of EC) and ANS (analysis of nutrient solution). The plant height in the early growth stage was not different among the treatments, but plant fresh weight and dry weight were higher in the MEC or the ANS than in the control. The growth including fruit number, fruit weight, and yield of tomato in the ANS as compared with the control was favorable. The EC of drained solution tended to decrease in the early growth stage, but that of drained solution increased in the late growth stage. It was low in the MEC and the ANS as compared with the control. The pH of drained solution was maintained by 6.2 to 6.5 throughout the growth period in the MEC and the ANS, but the pH of the control increased up to 7.2 at the late growth stage. The N and K concentrations of drained solution tended to decrease in the early growth stage, while those of P, Ca and Mg increased. In the late growth stage, concentrations of N, P, Ca and Mg tended to decrease, but that of K in the ANS was very low. Concentrations of N, P, K, Ca and Mg were lower in the MEC and the ANS than in the control.

• Journal of Nutrition and Health
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• v.6 no.1
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• pp.1-9
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• 1973

I. Subject of the Study: Studies on the economical nutrition supplement of cereal foods for the improvement in our notional eating habits. II. Purpose and Importance of the Study: 1. Our nation is confronted with the situation that the rice, a principal food, short of some essential amino acids, lysine and threonine, leads to imbalanced meals insufficient in the nutrient of protein, to bring many difficulties in the elevation of our national physique. 2. The shortage of even the rice imperfect in the nutrient of protein makes the import of lots of foreign rice inevitable. It is considered that the protein supplement and decrease in the consumption amount, of rice, is a serious key to the solution of our food difficulty, and then a way of the proetin supplement of rice through the addition of essential amino acid is to be rarely applied in the view of the our present finance and situation. 3. In the present experiment, therefore, it aims to the suggestion of an aspect of the improvement in our national eating habits guiding in the nutrition elevation which our nation can afford economically through the development of first, a way of the protein supplement by the mixture of cereals producted plentifully in our country, and second, a way of the decrease in the consumption amount and the improvement in the nutrition of rice through the substitution of the other cereals for rice. III. Contents of Scope of the Study: 1. Objects of the study: Objects of the study are the following three items; a) The nutrition supplement of rice through the mixture of cereals. Our nation makes mainly rice as a principal food, but practically many kinds of cereal are produced in our country. They contain different levels and qualities of each nutrient and they are different from one another in the kinds of essential amino acid consisting protein. For that reason, the mutual complement efficeincy of insufficient nutrients is observed through the mixture of cereals. b) The nutrition supplement of rice through the addition of superior protein sources to rice, a principal food. The development of rice as superior foods in the sense of nutrition is conducted through the risement in protein quality by the addition of protein sources in good quality, for example, fish flour (anchovy flour), egg powder, milk powder, and so on, and through the supplement of vitamins and minerals. c) The decrease in the consumption amount of rice through the substitution of the other cereals for rice, as a principal food. The compensation for the short amount of rice is made by the reduction in the consumption of rice through the discovery of a way of substitution of the other cereals for rice, as a principal food and of the settlement of problems in nutrition and finance subsequent to this. 2. Contents of the study: a) An ideal mixture-ratio of cereals is established for rats by feeding mixed foods(rice-barely or rice-wheat) containing 5%, 15%, 25%, 35% and 45% level of either barely or wheat. b) The nutritive value is determined in the whole subsititution of other foods for rice, and then, a way of the complement of over and under nutrients is devised. c) The ideal combination is investigated for rats through feeding mixed foods of main food, rice and supplement foods of protein sources, soy bean, fish flour, egg powder and milk Powder. d) According to results from the above three experiments, the concise functional test for men and the examination of economical property are made. 3. Scope of the study: a) The observation of the effect of each diet on the growth rate for rats. The growth rate of rats was observed for 15 groups of mixed foods of a main food, rice, and wheat flour, barley powder or soy bean powder, respectively, and 12 groups of wheat flour diets supplemented with $1{\sim}3%$ milk powder, and rice or wheat flour diets supplemented with 5% of milk powder, egg powder, fish flour or soy bean powder, respectively. b) The determination of food consumption. The food consumption was determined at weekly intervals for 27 kinds of diet described in a) item. c) The determination of food efficiency rate. The food efficiency rate for each diet was determined by calculation from the gained body weight and the food consumption amount at the same intervals described in b) item. d) The determination of protein efficiency rate. The protein efficiency rate for each diet was determined by calculation form gained body weight and the protein amount of the food consumption amount at the same intervals described c) item. e) The determination of the body component. The hematocrite and hemoglobin levels in the blood, total nitrogen in the serum, blood sugar, and lipids and glycogen in the liver were determined. f) The observation of nitrogen balance. As a means of the observation of nitrogen balance, the total nitrogen in the urine was determined. g) The analysis of economical property. The economical property was analyzed as the gained body weight to the amount equivalent to one won through the conversion of the food consumption amount into money. h) The functional test for men. The concise functional test for men was made in order to establish if the best diet for experimental animals can be applied to men. IV. Results of the Study: The national food product plan, nationwide nutritive enlightment and the improvement activities in our country eating habits, especially, mixed and powder food problems are to be significantly referred, and the following results must be applied. a) In the mixed foods of cereals, the mixed food of the rice-barley containing $5{\sim}15%$ level of barley is best in terms of nutrition. b) The addition of superior protein sources, egg, Bilk, soy bean, or fish, respectively to either rice or wheat flour makes a great risement in the nutritive value. c) The animal protein is more effective in the elevation of nutritive value of cereals. d) Rice takes the most nutritive operation and has the highest preference, among rice, wheat flour and barley. e) Wheat flour is more economical than rice in evaluation of the gained body weight to the regular money, and the addition of fish or soy bean is more economical than that of any other supplement food. But the above results are true of the range of nutrition and economical property. f) The study on the nutrition composition and barley will lead to the improvement in our national eating habits as mixed food of the rice-barley containing $5{\sim}15%$ level of barley is more nutritive. g) This study on the nutrition only for the growing animal can not be considered as a perfect and entire evaluation. Consequently, the perfect data for our national nutrition can be obtained from the experiment similar to this for the much longer period examining, in details, the growth rate, change of physical strength, mental and bodily change, average life span, and resistance ability to infectious diseases.

• Horticultural Science & Technology
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• v.19 no.1
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• pp.87-91
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• 2001

The aim of this study was to select the proper temperature for MA storage and to study the effect of sodium selenate ($Na_2SeO_4$) on storability of hydroponically grown sweet basil. Sweet basil was cultured with 1 fold using the nutrient solution developed by European Vegetable R & D Center in Belgium. Three-weeks before harvest, sodium selenate ($Na_2SeO_4$) was treated 2, 4, 6 and $8mg{\cdot}L^{-1}$ in the nutrient solution. Although electrolyte leakage of sweet basil was remarkably increased because of chilling injury at $5^{\circ}C$ MA storage, storage life was extended for three more days by selenium supplement. The weight loss was lower at $10^{\circ}C$ than at $5^{\circ}C$ and vitamin C content was higher at $10^{\circ}C$ than $5^{\circ}C$ storage treatment. Se treatment increased vitamin C content and decreased ion leakage in the plant. Accumulation of Se in the leaves of basil was increased with Se concentration of the nutrient solution. Se accumulation was $66{\mu}g{\cdot}g^{-1}$ dry mass at $2mg{\cdot}L^{-1}$ treatment and $422{\mu}g{\cdot}g^{-1}$ dry mass at $8mg{\cdot}L^{-1}$ treatment. The results suggest that storage at $10^{\circ}C$ is good for reduction of chilling injury and maintain marketability of basil. Less than $2mg{\cdot}L^{-1}$ sodium selenate treatment is acceptable for human health.

• Journal of the Korean Society of Industry Convergence
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• v.21 no.1
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• pp.9-16
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• 2018

Aloe gel layer is well known as raw materials of medicines and cosmetics due to their antioxidant and anti-inflammatory properties. In aloe gel extracting process, the outer part of the leaf was removed. It contains high quality of fiber and many nutrients. However, this part is thrown away and generally used as fertilizer. The purpose of this research was to examine the important nutrient of Aloe saponaria. Moreover, the feasibility of using aloe as a dietary supplement by feeding fermentation treatment of aloe was investigated. To do this, the aloe leaf was divided into several parts including leaf skin, bottom of the leaf, tip of the leaf, middle of the leaf, and leaf flesh. Then the saponin content were analyzed from each part. The extraction method was used to clarify the saponin content. The aloe then fermented to improve it benefit. The fermented Aloe then given as dietary food to group of pig. Finally, the appropriate feed level was determined and the pork meat quality was analyzed. The extraction of saponin shows that the highest concentration of saponin located on the skin of the leaf. The feeding experiment shows that there is no significant difference in pig growth without aloe dietary food and groups with aloe as dietary food. It was conclude that fermented aloe can replace the pigs normal feeder as an alternative feeding solution.

• Korean Journal of Environmental Agriculture
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• v.24 no.1
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• pp.24-28
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• 2005

Waste of the hydroponic solution from the plastic film house cultivation was recycled to grow the red pepper(Capsicum annum L.) in upland fields as supplement for plant nutrients and irrigation sources. Application of hydroponic waste solution increased the pH and EC of the soils, coupled with the increases in the concentrations of exchangeable cations(Ca, Mg, and K), total nitrogen, $NH_4-N,\;and\;NO_3-N$. Growth and yield of red pepper were highest when the treatment of chemical fertilizer(70%) was combined with hydroponic waste solution(30%). Amounts of the daily producing hydroponic waste solution were 2,880 L $ha^{-1}\;day^{-1}$ from the experimental facilities and this could irrigate $409.86m^2$ of area to compensate for the amount of water loss by evapotranspiration(3%). The overall results demonstrated that hydroponic waste solution could be recycled as plant nutrients and irrigation water resources for enhancing soil fertility and environmental quality.

• Journal of Microbiology and Biotechnology
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• v.27 no.7
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• pp.1281-1287
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• 2017

Bone morphogenetic protein-4 (BMP-4) is considered to have therapeutic potential for various diseases, including cancers; however, the high expression of biologically active recombinant human BMP-4 (rhBMP-4) needed for its manufacture for therapeutic purposes has yet to be established. In the current study, we established a recombinant Chinese hamster ovary (rCHO) cell line overexpressing rhBMP-4 as well as a production process using 7.5-l bioreactor (5 L working volume). The expression of the mature rhBMP-4 was significantly enhanced by recombinant furin expression. The combination of a chemically defined medium and a nutrient supplement solution for high expression of rhBMP-4 was selected and used for bioreactor cultures. The 11-day fed-batch cultures of the established rhBMP-4-expressing rCHO cells in the 7.5-L bioreactor produced approximately 32 mg/l of rhBMP-4. The mature rhBMP-4 was purified to homogeneity from the culture supernatant using a two-step chromatographic procedure, resulting in a recovery rate of approximately 55% and a protein purity greater than 95%. The N-terminal amino acid sequences and N-linked glycosylation of the purified rhBMP-4 were confirmed by N-terminal sequencing and de-N-glycosylation analysis, respectively. The mature purified rhBMP-4 has been proved to be functionally active, with an effective dose concentration of $EC_{50}$ of 2.93 ng/ml.